Conversion of Roux-en-Y gastric bypass to single anastomosis duodenal ileal bypass with sleeve gastrectomy with gastrogastric jejunal bridge.

Surgical conversion of Roux-en-Y gastric bypass (RYGB) to one anastomosis duodenal switch with sleeve gastrectomy (SADI-S), can be effective, when there is obesity recidivism, but surgically challenging. This case report video aims to detail the technical modifications that simplifies this conversion, in one stage. This video article demonstrates the conversion of RYGB to SADI-S using a jejunal bridge to facilitating the gastro-gastric reconnection. Surgical conversion was done laparoscopically, firstly removing the fundus, gastric body and the proximal part of the antrum. The gastrojejunal (GJ) anastomosis from the previous RYGB was preserved and the jejunal alimentary limb that follows was transected, 8cm distal to the GJ anastomosis, and anastomosed, at this level, with the antrum. The remaining alimentary limb was removed, until the jejuno-jejuno anastomosis, from the previous RYGB. The interposition of a segment of jejunal alimentary limb between the gastric bypass pouch and the antrum, has shown to be safe and feasible in RYGB conversion to SADI-S, without complications. Not reconnecting the remnant jejunal alimentary limb to the intestinal transit, but removing it, makes the procedure shorter and safer.

Paracoccidioidomicose: perfil clínico e epidemiológico de pacientes internados em Passo Fundo - RS / Paracoccidioidomycosis: clinical and epidemiological profile of patients hospitalized in Passo Fundo ­ RS

A paracoccidioidomicose não é uma doença de notificação obrigatória apesar de sua relevância na América Latina, por isso as estimativas de prevalência, incidência e morbidade dessa micose são baseadas em relatórios de levantamentos epidemiológicos, séries de casos, registros de hospitalização e dados de mortalidade. O objetivo desse trabalho foi descrever aspectos relacionados com o paciente, evolução da doença, confirmação diagnóstica e tratamento de casos confirmados de paracoccidioidomicose atendidos em um hospital de ensino do sul do Brasil. Foram coletadas informações de prontuários de 27 pacientes com diagnóstico de paracoccidioidomicose confirmado no período de 2010 até 2019. O perfil prevalente foi de um paciente do sexo masculino, com idade média de 53 anos, envolvido com atividades laborais diversas, de procedência urbana, imunocompetente e sem comorbidades, tabagista, mas não etilista. Para a maioria dos casos o acometimento inicial foi pulmonar, com importante envolvimento do sistema linfático no percurso da doença. A observação microscópica das estruturas fúngicas patognomônicas em amostras de biópsia, aspirado linfonodal e escarro foi o método mais utilizado para confirmar a suspeita clínica. O itraconazol foi a primeira opção de tratamento, seguido da anfotericina B. [au]

Paracoccidioidomycosis is not a notifiable disease despite its relevance in Latin America, so estimates of prevalence, incidence and morbidity of this mycosis are based on reports of epidemiological surveys, case series, hospitalization records and mortality data. The objective of this study was to describe aspects related to the patient, disease evolution, diagnostic confirmation and treatment of confirmed cases of paracoccidioidomycosis treated at a teaching hospital in southern Brazil. Information was collected from the medical records of 27 patients diagnosed with paracoccidioidomycosis confirmed in the period from 2010 to 2019. The prevalent profile was a male patient, with a mean age of 53 years, involved in various work activities, of urban origin, immunocompetent and without comorbidities, smoker, but non-alcoholic. For most cases, the initial involvement was pulmonary, with significant involvement of the lymphatic system in the course of the disease. Microscopic observation of pathognomonic fungal structures in biopsy samples, lymph node aspirate and sputum was the most used method to confirm the clinical suspicion. Itraconazole was the first treatment option, followed by amphotericin B. [au]

Conhecimento dos cirurgiões dentistas sobre o uso de anestésicos locais em pacientes: diabéticos, hipertensos, cardiopatas, gestantes e com hipertireoidismo / Knowledge of surgeons dentists about the use of local anesthetics in patients: diabetic, hypertension, heart disease, pregnant women and with hyperthyroidism

Anestésicos locais, contendo ou não vasoconstritores, são utilizados para realização da maioria dos procedimentos odontológicos. Porém, seu uso inadequado, principalmente em casos de alteração sistêmica, pode acarretar sérios riscos para a saúde do paciente. O objetivo deste estudo foi avaliar o nível de conhecimento dos cirurgiões dentistas (CDs) com relação à utilização de anestésicos locais, frente à pacientes especiais com diabetes, hipertensão, cardiopatias, gestantes e pacientes com hipertireoidismo. O estudo teve um delineamento quantitativo do tipo transversal com dados coletados através de questionário adaptado pela equipe como único instrumento para coleta de dados. Os dados foram submetidos à análise estatística descritiva e inferencial. De 200 participantes, 17% eram acadêmicos formandos em odontologia, e, dentre os formados, a maioria (34 %) tinha de 1 a 5 anos ou de 6 a 10 anos (16%) de formação. Do total de participantes graduados, 43% não possuíam especialização e 57% eram especialistas. Os resultados demostraram baixa taxa de acertos global (62%) entre os participantes. Quanto as categorias de pacientes especiais, observamos bom nível de conhecimento sobre o uso de anestésicos em pacientes diabéticos (79%), hipertensos (73%) e nível razoável para gestantes (65%) e pacientes com hipertireoidismo (62%). Entretanto, 60% erraram as questões relacionadas a cardiopatias. Ademais, foi insuficiente o percentual daqueles que afirmaram aferir a P.A no início dos atendimentos (45%), assim como, daqueles que consideram-se preparados para atender pacientes especiais (36%). Concluiu-se que o nível de conhecimento dos CDs relacionado ao atendimento de pacientes especiais é insuficiente havendo diferença significativa entre especialistas e clínicos(AU)

Local anesthetics, with or without vasoconstrictor are used to perform the vast majority of procedures in dentistry. However, improperly use it can lead to serious risks to patient's health, especially if it has some systemic disorders. The aim of this study was evaluate the level of dentistry's knowledge about use of local anesthetics in special-needs patients with diabetes, hypertension, heart disease, pregnant and patients with hyperthyroidism. The study was a quantitative cross-sectional design and the data were collected through a questionnaire adapted by the team as the only tool for data collection. Data were subjected to descriptive and inferential statistical analysis. Of the total 200 participants, 17% were students from the last year of Odontology (17%), most had 1-5 years of graduation (34%) or 6-10 years of graduation (16%). Of the total graduated participants, 43% were non-specialist and 57% were specialists. The results show a low overall hit rate (62%) among all participants. Among the special-needs patients' categories we observed a good level of knowledge about the use of anesthetics in patients with diabetes (79%) and hypertension (73%) and reasonable level for pregnant (65%) and patients with hyperthyroidism (62%). However, 60% erred issues related to heart disease. It was also lower the percentage who said measure BP (blood pressure) before start all treatment (45%) and who claimed to feel prepared to attend special-needs patients (36%). It was concluded that dentistry knowledge level regarding the care of special-needs patients is insufficient and has significant difference between non-specialist and specialists(AU)

Avaliação da microinfiltração marginal utilizando um sistema adesivo autocondicionante / Marginal microleakage assessment using a self-etching adhesive system

Este trabalho avaliou, in vitro, a capacidade seladora proporcionada pelo sistema adesivo autocondicionante Single Bond Universal (3M/ESPE) enfocando sua performance em relação à superfície de esmalte. Foram utilizados 20 pré-molares humanos extraídos por razões ortodônticas, reservando 5 dentes para projeto piloto, e os demais divididos em 2 grupos: Grupo ECOM: com condicionamento ácido do esmalte + Single Bond Universal e Grupo ESEM: aplicação exclusiva do sistema adesivo autocondicionante. Foram confeccionados preparos cavitários Classe II padronizados mesial e distal, seguido de limpeza da cavidade, protocolo adesivo seguindo as recomendações do fabricante e restauração em resina composta nanopartículada Z350XT (3M/ESPE) cor A3E pela técnica incremental e fotopolimerização por 20 segundos cada incremento. Em seguida, os espécimes foram impermeabilizados e imersos em solução aquosa de azul de metileno 1% por 24 horas e então lavados. Para coleta dos dados, os espécimes foram seccionados longitudinalmente totalizando 30 amostras, as quais foram analisadas em um contador de colônias manual (Phoenix CP 608) por 2 avaliadores, classificando os graus de infiltração em escores. Os dados foram submetidos à análise estatística pelo teste não paramétrico de Mann Whitney, mostrando que não houve diferença estatisticamente considerável entre os dois grupos (p= 0,89). O estudo concluiu que o condicionamento ácido do esmalte previamente à aplicação do sistema adesivo autocondicionante não reduziu a presença de microinfiltração marginal, não apresentando diferenças estatísticas significantes entre os grupos (AU).

This work evaluated in vitro the sealing capacity provided by the self-etching adhesive system Single Bond Universal (3M/ESPE) focusing on its performance in relation to the enamel surface. Twenty human premolars extracted for orthodontic reasons were used, five teeth were set aside for the pilot project, and the others were divided into two groups: ECOM Group: with acid enamel conditioning + Single Bond Universal and ESEM Group: exclusive application of self-etching adhesive system. Distal and mesial standardized Class II cavity preparations were made and subsequently cavity cleaning, adhesive protocol following manufacturer recommendations and restoration of nanoparticle composite resin Z350XT (3M/ESPE) color A3E by the incremental technique and photopolymerization for 20 seconds for each increment. After that the specimens were sealed and immersed in an aqueous solution of methylene blue 1% for 24 hours and then washed. In order to collect data, the specimens were longitudinally sectioned totaling 30 samples that were analyzed in a manual colony counter (Phoenix CP 608) by 2 reviewers, classifying the infiltration degrees in scores. Data was submitted to statistical analyses by the non-parametric Mann Whitney, showing that there was no considerable statistic difference between the two groups (p= 0.89). The study concluded that enamel acid etching prior to self-etching adhesive system application did not reduce the presence of marginal microleakage and did not present significant statistical differences between groups (AU).

Immunological correlates of favorable long-term clinical outcome in multiple sclerosis patients after autologous hematopoietic stem cell transplantation.

High dose immunosuppression followed by autologous hematopoietic stem cell transplantation (AHSCT) induces prolonged clinical remission in multiple sclerosis (MS) patients. However, how patient immune profiles are associated with clinical outcomes has not yet been completely elucidated. In this study, 37 MS patients were assessed for neurological outcomes, thymic function and long-term immune reconstitution after AHSCT. Patients were followed for a mean (SD) of 68.5 (13.9) months post-transplantation and were retrospectively clustered into progression- and non-progression groups, based on Expanded Disease Status Scale (EDSS) outcomes at last visit. After AHSCT, both patient groups presented increased regulatory T-cell subset counts, early expansion of central- and effector-memory CD8(+)T-cells and late thymic reactivation. However, the non-progression group presented early expansion of PD-1(+)CD8(+)T-cells and of PD-1-expressing CD19(+) B-cells. Here, we suggest that along with increased numbers of regulatory T-cell subsets, PD-1 inhibitory signaling is one possible immunoregulatory mechanism by which AHSCT restores immune tolerance in MS patients.

Neutrophil Extracellular Traps Induce Organ Damage during Experimental and Clinical Sepsis.

Organ dysfunction is a major concern in sepsis pathophysiology and contributes to its high mortality rate. Neutrophil extracellular traps (NETs) have been implicated in endothelial damage and take part in the pathogenesis of organ dysfunction in several conditions. NETs also have an important role in counteracting invading microorganisms during infection. The aim of this study was to evaluate systemic NETs formation, their participation in host bacterial clearance and their contribution to organ dysfunction in sepsis. C57Bl/6 mice were subjected to endotoxic shock or a polymicrobial sepsis model induced by cecal ligation and puncture (CLP). The involvement of cf-DNA/NETs in the physiopathology of sepsis was evaluated through NETs degradation by rhDNase. This treatment was also associated with a broad-spectrum antibiotic treatment (ertapenem) in mice after CLP. CLP or endotoxin administration induced a significant increase in the serum concentrations of NETs. The increase in CLP-induced NETs was sustained over a period of 3 to 24 h after surgery in mice and was not inhibited by the antibiotic treatment. Systemic rhDNase treatment reduced serum NETs and increased the bacterial load in non-antibiotic-treated septic mice. rhDNase plus antibiotics attenuated sepsis-induced organ damage and improved the survival rate. The correlation between the presence of NETs in peripheral blood and organ dysfunction was evaluated in 31 septic patients. Higher cf-DNA concentrations were detected in septic patients in comparison with healthy controls, and levels were correlated with sepsis severity and organ dysfunction. In conclusion, cf-DNA/NETs are formed during sepsis and are associated with sepsis severity. In the experimental setting, the degradation of NETs by rhDNase attenuates organ damage only when combined with antibiotics, confirming that NETs take part in sepsis pathogenesis. Altogether, our results suggest that NETs are important for host bacterial control and are relevant actors in the pathogenesis of sepsis.

Prevalência de artralgia em indivíduos obesos no pré e pós-operatório tardio de cirurgia bariátrica / Prevalence of arthralgia in obese individuals in the pre and postoperative of bariatric surgery

Objetivos: analisar a prevalência de artralgia no pré e no pós-operatório tardio de cirurgia bariátrica, comparar o índice de massa corporal (IMC) dosindivíduos obesos nos períodos pré e pós-operatório, verificar a associação entre teste de caminhada de seisminutos e artralgia e avaliar a correlação entre teste de caminhada de seis minutos e IMC. Métodos: estudo de coorte retrospectivo, compostopor 43 indivíduos obesos que realizaram avaliação no período pré e pós-operatório tardio de cirurgiabariátrica. Os dados foram expressos como média e desvio padrão, e descritos através de gráfico e tabela de frequência com proporções. As análises estatísticas foram realizadas pelos testes t de Student. A correlaçãoentre IMC e distância percorrida foi analisada por regressão linear e coeficiente de Pearson. Resultados: artralgia foi referida por 83,7% dospacientes no pré-operatório e por 37,2% pacientes no pós-operatório de seis meses. A diferença entre o IMC pré e pós-operatório mostrou-se significativa(p<0,001). Quando comparada a distância percorrida e o IMC tanto no pré quanto no pós-operatório, obtevesediferença significativa. No período pós-operatório houve um aumento da distância percorrida no teste de caminhada de seis minutos, estando esta associada à redução do IMC, mas não houve associação entre distância percorrida no teste de caminhada de seisminutos e presença de artralgia.Conclusões: os pacientes obesos submetidos à cirurgia bariátrica apresentaram uma alta prevalênciade artralgia no período pré-operatório, havendo diminuição da mesma no período pós-operatório,bem como redução do índice de massa corporal. A ausência de associação entre distância percorrida no teste de caminhada de seis minutos e presença deartralgia no pós-operatório sugere que a artralgia não foi o principal fator limitante para a caminhada nos indivíduos obesos após cirurgia bariátrica.

Aims: To analyze the prevalence of joint pain, to compare the body mass index (BMI), to verify the associationbetween BMI and six-minute walk test, and the association between joint pain in obese patients in pre and post surgerysix months after bariatric surgery.Methods: A retrospective cohort study was performed. The subjects were 43 obese individuals that performed thepre and six-month post surgery evaluation. Student's t test was used for statistical analyses. The relation between BMI and the distance walked was analyzed by linear regression.The data were expressed as average standard deviations, and described through graphics and table of frequency with proportions. Results: Joint pain was noticed in 83.7% in the presurgerygroup and in 37.2% in the post-surgery group after six months. The difference in BMI between pre and post surgery was significant (p<0.001). A significant difference was noticed when walked distance and BMI were comparedboth in pre and post surgery. In the post-surgery there was a rise in the distance walked during six minute walk test, which was associated to the reduction of BMI, but an association between walked distance and presence of joint pain was not found. Conclusions: Obese patients subjected to bariatric surgery had a high prevalence of joint pain in the presurgery period that decreased in the post-surgery, like body mass index. The lack of association between the walkeddistance during six minute walk test and presence of joint pain suggests that this was not the main limiting factor forwalking in these subjects.

Diagnóstico e estudo sorológico da infecção pelo parvovírus canino em cães de Passo Fundo, Rio Grande do Sul, Brasil / Diagnosis and serological study of canine parvovirus infection in dogs from Passo Fundo, Rio Grande do Sul, Brazil

A reação em cadeia da polimerase (PCR) e o ensaio de hemaglutinação (HA) foram comparados para a identificação de parvovírus canino (CPV) em fezes de cães com gastrenterite. A prevalência de anticorpos anti-CPV em uma população de cães sem histórico de vacinação foi avaliada pelo ensaio de inibição da hemaglutinação (HI). A variabilidade fenotípica entre uma amostra vacinal e isolados de campo foi investigada utilizando soro hiperimune. Trinta amostras fecais foram obtidas de cães com diarréia e submetidas à PCR e ao HA, e 185 amostras de soro de cães foram submetidas à HI para detectar anticorpos anti-CPV. Considerando-se somente como positivas as amostras que apresentaram HA na diluição igual ou superior a 1:64, detectou-se CPV em 9 (30 por cento) das amostras fecais. Nove amostras fecais apresentaram HA nas diluições entre 1:2 e 1:32 e foram consideradas negativas nesse teste. Todas as amostras que apresentaram HA na diluição igual ou superior a 1:64 foram positivas pela PCR e, das nove amostras que apresentaram HA nas diluições entre 1:2 e 1:32, seis também foram positivas para CPV na amplificação pela PCR. A pesquisa sorológica de amostras de soros caninos indicou que 178 (96,2 por cento) cães tiveram contato prévio com o vírus. Os soros hiperimunes produzidos em cobaias contra a cepa vacinal e dois isolados de campo indicaram possíveis diferenças fenotípicas entre isolados.(AU)

The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine parvovirus (CPV) in feces from young dogs with gastroenteritis. The hemaglutination inhibition (HI) assay was used to detect the prevalence of anti-CPV antibodies in a non-vaccinated dog population. In addition, hiperimmune serum was used to investigate the phenotypic variability of a vaccine strain and two field isolates of CPV. Thirty fecal samples obtained from dogs with diarrhea were submitted to PCR and HA, and 185 serum samples were submitted to HI to detect anti-CPV antibodies. Nine (30 percent) of the samples demonstrated HA on fecal dilutions equal to or above 1:64 and were considered positive by this test; nine (30 percent) fecal samples had HA activity on dilution from 1:2 to 1:32 and were considered negative, and the remaining samples were negative. All samples with HA activity at dilutions above 1:64 were also positive to PCR and, out of the nine samples with HA activity at dilutions between 1:2 and 1:32, six were also positive by PCR. Serological analysis of the dog serum samples indicated that 178 (96,2 percent) of the dogs had previous contact with the virus. Hiperimmune serum indicated possible phenotypic differences among isolates, in that different HI titers were obtained following cross-HI assay.(AU)

Diagnóstico e estudo sorológico da infecção pelo parvovírus canino em cães de Passo Fundo, Rio Grande do Sul, Brasil / Diagnosis and serological study of canine parvovirus infection in dogs from Passo Fundo, Rio Grande do Sul, Brazil

A reação em cadeia da polimerase (PCR) e o ensaio de hemaglutinação (HA) foram comparados para a identificação de parvovírus canino (CPV) em fezes de cães com gastrenterite. A prevalência de anticorpos anti-CPV em uma população de cães sem histórico de vacinação foi avaliada pelo ensaio de inibição da hemaglutinação (HI). A variabilidade fenotípica entre uma amostra vacinal e isolados de campo foi investigada utilizando soro hiperimune. Trinta amostras fecais foram obtidas de cães com diarréia e submetidas à PCR e ao HA, e 185 amostras de soro de cães foram submetidas à HI para detectar anticorpos anti-CPV. Considerando-se somente como positivas as amostras que apresentaram HA na diluição igual ou superior a 1:64, detectou-se CPV em 9 (30 por cento) das amostras fecais. Nove amostras fecais apresentaram HA nas diluições entre 1:2 e 1:32 e foram consideradas negativas nesse teste. Todas as amostras que apresentaram HA na diluição igual ou superior a 1:64 foram positivas pela PCR e, das nove amostras que apresentaram HA nas diluições entre 1:2 e 1:32, seis também foram positivas para CPV na amplificação pela PCR. A pesquisa sorológica de amostras de soros caninos indicou que 178 (96,2 por cento) cães tiveram contato prévio com o vírus. Os soros hiperimunes produzidos em cobaias contra a cepa vacinal e dois isolados de campo indicaram possíveis diferenças fenotípicas entre isolados.

The polymerase chain reaction (PCR) and hemaglutination (HA) assay were used to detect canine parvovirus (CPV) in feces from young dogs with gastroenteritis. The hemaglutination inhibition (HI) assay was used to detect the prevalence of anti-CPV antibodies in a non-vaccinated dog population. In addition, hiperimmune serum was used to investigate the phenotypic variability of a vaccine strain and two field isolates of CPV. Thirty fecal samples obtained from dogs with diarrhea were submitted to PCR and HA, and 185 serum samples were submitted to HI to detect anti-CPV antibodies. Nine (30 percent) of the samples demonstrated HA on fecal dilutions equal to or above 1:64 and were considered positive by this test; nine (30 percent) fecal samples had HA activity on dilution from 1:2 to 1:32 and were considered negative, and the remaining samples were negative. All samples with HA activity at dilutions above 1:64 were also positive to PCR and, out of the nine samples with HA activity at dilutions between 1:2 and 1:32, six were also positive by PCR. Serological analysis of the dog serum samples indicated that 178 (96,2 percent) of the dogs had previous contact with the virus. Hiperimmune serum indicated possible phenotypic differences among isolates, in that different HI titers were obtained following cross-HI assay.

Prevalence of enzootic bovine leukosis, bovine viral diarrhea, infectious bovine rhinotracheitis and bovine neosporosis in 26 dairy cattle farms from the northeast region of rio grande do sul , brazil / Prevalência de leucose enzoótica bovina, diarréia viral bovina rinotraqueíte infecciosa bovina e neosporose bovina em 26 propriedades leiteiras da região nordeste do rio grande do sul, brasil

The objective was to investigate the frequency of anti-Brucella abortus agglutinins in goats and sheep of the backlands of the State of Pernambuco, Brazil. 700 samples of sanguine serums were processed, of which 340 were of the goat (115 males and 225 females) and 360 (136 males and 224 females) sheep. The technique of the Tamponed Acidified Antigen (AAT) dyed with Bengalese Rose (BR) was used. Of the 340 samples of goat evaluated two (0.6%) were reactive to AAT. Significant associations were not observed for the variable age group (p = 0.430); race (p = 0.936) and sex (p = 0.562). Of the 360 sheep samples, nine (2.5%) were reactive. There also was no significant association between the analyzed variables and the seropositiveness for brucellosis: age group (p = 0.522); race (p = 0.576) and sex (p = 0.461). Significant association was observed (p = 0.042) among the studied species and seropositiveness for brucellosis in the investigated animals. The seropositiveness for Brucella abortus in goats and sheep was traced for the first time in the Sertão (dry interior region, backlands) of Pernambuco, fact that can hinder the success of the National Program of Control and Erradication of Brucellosis, due to the fact that it is common to raise small ruminants with bovines in this area, besides representing risks to Public Health.  KEY WORDS: Brucellosis, ovines, caprines, small rumin

Objetivou-se investigar a freqüência de aglutininas anti-Brucella abortus em caprinos e ovinos do Sertão do Estado de Pernambuco, Brasil. Foram processadas 700 amostras de soros sangüíneos, das quais 340 eram da espécie caprina (115 machos e 225 fêmeas) e 360 (136 machos e 224 fêmeas) ovina. Empregou-se a técnica do antígeno acidificado tamponado (AAT) corado com rosa bengala (RB). Das 340 amostras de caprinos avaliadas, duas (0,6%) foram reagentes ao AAT. Não se observaram associações significativas para as variáveis faixa etária (p= 0,430), raça (p= 0,936) e sexo (p= 0,562). Das 360 amostras de ovinos, nove (2,5%) foram reagentes. Também não houve associação significativa entre as variáveis analisadas e a soropositividade para brucelose: faixa etária (p= 0,522); raça (p= 0,576) e sexo (p= 0,461). Verificou-se associação significativa (p= 0,042) entre as espécies estudadas e soropositividade para brucelose nos animais investigados. A soropositividade para Brucella abortus em caprinos e ovinos foi descrita pela primeira vez no Sertão de Pernambuco, fato que pode dificultar o sucesso do Programa Nacional de Controle e Erradicação da Brucelose, tendo em vista que nessa região é comum a criação consorciada de pequenos ruminantes com bovinos, além de representar riscos à Saúde Pública.PALAVRAS-CHAVES: Brucelose, ovinos, caprinos, pequenos ruminantes, sorodiagnóstico.

PREVALENCE OF ENZOOTIC BOVINE LEUKOSIS, BOVINE VIRAL DIARRHEA, INFECTIOUS BOVINE RHINOTRACHEITIS AND BOVINE NEOSPOROSIS IN 26 DAIRY CATTLE FARMS FROM THE NORTHEAST REGION OF RIO GRANDE DO SUL , BRAZIL / PREVALÊNCIA DE LEUCOSE ENZOÓTICA BOVINA, DIARRÉIA VIRAL BOVINA RINOTRAQUEÍTE INFECCIOSA BOVINA E NEOSPOROSE BOVINA EM 26 PROPRIEDADES LEITEIRAS DA REGIÃO NORDESTE DO RIO GRANDE DO SUL, BRASIL

The objective was to investigate the frequency of anti-Brucella abortus agglutinins in goats and sheep of the backlands of the State of Pernambuco, Brazil. 700 samples of sanguine serums were processed, of which 340 were of the goat (115 males and 225 females) and 360 (136 males and 224 females) sheep. The technique of the Tamponed Acidified Antigen (AAT) dyed with Bengalese Rose (BR) was used. Of the 340 samples of goat evaluated two (0.6%) were reactive to AAT. Significant associations were not observed for the variable age group (p = 0.430); race (p = 0.936) and sex (p = 0.562). Of the 360 sheep samples, nine (2.5%) were reactive. There also was no significant association between the analyzed variables and the seropositiveness for brucellosis: age group (p = 0.522); race (p = 0.576) and sex (p = 0.461). Significant association was observed (p = 0.042) among the studied species and seropositiveness for brucellosis in the investigated animals. The seropositiveness for Brucella abortus in goats and sheep was traced for the first time in the Sertão (dry interior region, backlands) of Pernambuco, fact that can hinder the success of the National Program of Control and Erradication of Brucellosis, due to the fact that it is common to raise small ruminants with bovines in this area, besides representing risks to Public Health.  KEY WORDS: Brucellosis, ovines, caprines, small rumin

Objetivou-se investigar a freqüência de aglutininas anti-Brucella abortus em caprinos e ovinos do Sertão do Estado de Pernambuco, Brasil. Foram processadas 700 amostras de soros sangüíneos, das quais 340 eram da espécie caprina (115 machos e 225 fêmeas) e 360 (136 machos e 224 fêmeas) ovina. Empregou-se a técnica do antígeno acidificado tamponado (AAT) corado com rosa bengala (RB). Das 340 amostras de caprinos avaliadas, duas (0,6%) foram reagentes ao AAT. Não se observaram associações significativas para as variáveis faixa etária (p= 0,430), raça (p= 0,936) e sexo (p= 0,562). Das 360 amostras de ovinos, nove (2,5%) foram reagentes. Também não houve associação significativa entre as variáveis analisadas e a soropositividade para brucelose: faixa etária (p= 0,522); raça (p= 0,576) e sexo (p= 0,461). Verificou-se associação significativa (p= 0,042) entre as espécies estudadas e soropositividade para brucelose nos animais investigados. A soropositividade para Brucella abortus em caprinos e ovinos foi descrita pela primeira vez no Sertão de Pernambuco, fato que pode dificultar o sucesso do Programa Nacional de Controle e Erradicação da Brucelose, tendo em vista que nessa região é comum a criação consorciada de pequenos ruminantes com bovinos, além de representar riscos à Saúde Pública.PALAVRAS-CHAVES: Brucelose, ovinos, caprinos, pequenos ruminantes, sorodiagnóstico.