RESUMEN
In male patients with coronary atherosclerosis without acute coronary syndrome, the levels of inflammatory-destructive biomarkers of atherosclerotic plaque instability depended on the severity and dissemination of coronary atherosclerosis. The highest levels of C-reactive protein and matrix metalloproteinase 3 were found in men with atherosclerotic involvement of all three main coronary arteries, primarily their middle and distal segments, and in men with predominance of low-grade stenoses (<50%) of coronary arteries in areas of atherosclerotic plaques.
Asunto(s)
Biomarcadores/sangre , Enfermedad de la Arteria Coronaria/sangre , Estenosis Coronaria/sangre , Síndrome Coronario Agudo/sangre , Adulto , Anciano , Proteína C-Reactiva/metabolismo , Vasos Coronarios/metabolismo , Vasos Coronarios/patología , Humanos , Masculino , Metaloproteinasa 3 de la Matriz/sangre , Persona de Mediana Edad , Placa Aterosclerótica/sangre , Placa Aterosclerótica/patologíaRESUMEN
We studied men with coronary atherosclerosis without acute coronary syndrome and determined typical valuable parameters of inflammatory (tumor necrotic factor, antagonist of receptor to interleukin [IL] 1, IL 6, IL 8, monocytes chemotactic protein 1, endothelial monocytes activating protein II), and destructive (matrix metalloproteinase [MMP] 3, MMP 7, MMP 9, tissue inhibitor of metalloproteinase) processes at consecutive stages of formation of coronary atherosclerotic plaque: "normal intimal tissue --> lipid stain --> early stable plaque --> unstable vulnerable plaque <--> stable plaque with fibrosis", and in 3 types of unstable plaques (lipid type, inflammatory erosive type, necrotic type).
Asunto(s)
Enfermedad de la Arteria Coronaria/enzimología , Enfermedad de la Arteria Coronaria/metabolismo , Citocinas/metabolismo , Metaloproteasas/metabolismo , Adulto , Anciano , Biomarcadores/metabolismo , Enfermedad de la Arteria Coronaria/patología , Vasos Coronarios/enzimología , Vasos Coronarios/metabolismo , Vasos Coronarios/patología , Humanos , Inflamación/metabolismo , Masculino , Persona de Mediana Edad , Necrosis , Túnica Íntima/enzimología , Túnica Íntima/metabolismo , Túnica Íntima/patología , Túnica Media/enzimología , Túnica Media/metabolismo , Túnica Media/patologíaRESUMEN
Oxidative and antioxidant parameters (content of LPO products and oxidized proteins, initial level of paraoxonase, content of alpha-tocopherol, retinol, and beta-carotene) were studied at different stages of atherosclerotic foci development in coronary arteries: intact intima, lipid spot, stable young plaque, unstable plaque, stable plaque with fibrosis/ calcinosis, and in various types of unstable plaques. The most typical sign of unstable plaques is high level of LPO products and low retinol content.
Asunto(s)
Antioxidantes/metabolismo , Aterosclerosis/metabolismo , Oxidantes/metabolismo , Adulto , Anciano , Arildialquilfosfatasa/metabolismo , Aterosclerosis/patología , Vasos Coronarios/metabolismo , Vasos Coronarios/patología , Humanos , Masculino , Malondialdehído/metabolismo , Persona de Mediana Edad , Oxidación-Reducción , Espectrometría de Fluorescencia , Vitamina A/metabolismo , alfa-Tocoferol/metabolismo , beta Caroteno/metabolismoRESUMEN
We studied parameters of inflammatory (tumor necrosis factor a, antagonist of interleukin-1 receptor, interleukin-6 , interleukin-8, C-reactive protein) and destructive (matrix metalloproteinases type 3 and 9, tissue inhibitor of metalloproteinase type 1) processes in dynamics of sequential stages of development of atherosclerotic foci in coronary arteries: unchanged intimal tissue --> lipid stain/streak --> stable young plaque --> unstable vulnerable plaque with inclination to ulceration of rupture --> stable plaque with fibrosis/calcinosis, and in various types of unstable plaques in men with coronary atherosclerosis. Characteristic for unstable plaques parameters of inflammatory activity were elevated levels of interleukins (IL) 6 and 8, C-reactive protein (CRP), of destructive activity -- elevated level of metalloproteinases-9. In inflammatory erosive type of unstable plaques (lowered level of antagonist of interleukin-1 receptor and elevated level of CRP) and in lipid type (elevated levels of IL-6, IL-8 and CRP) inflammatory activity was dominating compared with necrotic type in which dominated destructive activity (elevated level of tumor necrosis factor a and lowered level of tissue inhibitor of metalloproteinase type 1).
Asunto(s)
Arteriosclerosis/metabolismo , Proteína C-Reactiva/metabolismo , Estenosis Coronaria/metabolismo , Vasos Coronarios/metabolismo , Inflamación/metabolismo , Interleucinas/metabolismo , Metaloproteasas/metabolismo , Adulto , Anciano , Arteriosclerosis/complicaciones , Arteriosclerosis/patología , Biomarcadores/metabolismo , Estenosis Coronaria/etiología , Estenosis Coronaria/patología , Vasos Coronarios/patología , Ensayo de Inmunoadsorción Enzimática , Humanos , Inflamación/patología , Masculino , Persona de Mediana Edad , Índice de Severidad de la EnfermedadRESUMEN
We studied parameters of structural and oxidative modification of low density lipoproteins (LDL) in 80 men aged 35 - 65 years with coronary atherosclerosis verified at coronary angiography in comparison with 80 men of the same age without ischemic heart disease. Biochemical studies included determination of parameters of lipid blood composition by enzymatic methods, subfractional LDL profile by electrophoresis in 2 - 16% gradient polyacrylamide gel, degree of oxidative modification of lipid (lipid peroxidation products) and apoprotein (carbonyl groups) components of LDL by fluorometric and spectrophotometric methods. In men with coronary atherosclerosis we revealed elevated level of small dense LDL and substantially oxidized lipid and apoprotein components of these particles. Independent association of structural and oxidative modification was shown.