Electromyographic analysis of masseter and temporal muscles, bite force, masticatory efficiency in medicated individuals with schizophrenia and mood disorders compared with healthy controls.

This study aimed to comparatively analyse the electromyographic activity of the masseter and temporal muscles at rest and during mandible postural clinical conditions (right and left laterality, protrusion and maximum voluntary contraction), right and left maximum molar bite forces and the masticatory efficiency of individuals with schizophrenia or mood disorders - all medicated (medicated groups) compared with control group (healthy volunteers) via electromyography. Individuals were distributed into three groups: Group I (Schizophrenia - 20 individuals), Group II (mood disorders - 20 individuals) and Group III (Control - 40 individuals). Basically, the results were only statistically significant for the clinical mandible conditions and bite force. The most unsatisfactory results were observed in the medicated groups in relation to the control group. The group with mood disorders obtained the most unsatisfactory results compared with the group with schizophrenia. It was suggested by these observations that the association of mood disorders and schizophrenia with medication has negatively affected the stomatognathic system in relation to controls when the electromyography and bite force were used for the analysis.

Implant-supported prosthesis following Brånemark protocol on electromyography of masticatory muscles.

This research evaluated the effects of Brånemark protocol on electromyography of the masseter and temporal muscles. The patients were divided into two groups: Group I: patients who wore an implant-supported prosthesis in the mandibular arch following Brånemark protocol, and maxillary removable complete dentures; Group II: dentate individuals (control). Electromyography was carried out at rest, right (RL) and left (LL) laterality, protrusion and maximum voluntary contraction (MVC). Data were compared by t-test. At rest, a higher electromyographic (EMG) activity was observed in Group I, and the values were significant in the right masseter and left temporal muscles. In RL, there were statistically significant differences for right masseter (P < 0·01), left masseter and temporal muscles and for the left temporal muscle in LL (P < 0·05). In protrusion, Group I presented a higher EMG activity, and there was a statistically significant difference for the right masseter muscle (RM) (P < 0·05). In MVC, the EMG values were higher in Group II (control), but significant just for the right temporal muscle (P < 0·05). In conclusion, individuals with mandibular fixed dentures supported according to the Brånemark protocol and maxillary removable complete dentures showed a higher activity of masticatory muscles during the mandibular postural clinical conditions examined; however, in the MVC, Group I presented lower values when compared to dentate group. Despite presenting different EMG values in all of the clinical conditions, both groups showed similar EMG patterns of muscle activation which demonstrates that the proposed treatment can be considered as a good option for oral rehabilitation.

Ageing and surface EMG activity patterns of masticatory muscles.

The purpose of this study was to evaluate the influence of age on the electromyographic activity of masticatory muscles. All volunteers were Brazilian, fully dentate (except for Group I - mixed dentition), Caucasian, aged 7-80, and divided into five groups: I (7-12 years), II (13-20 years), III (21-40 years), IV (41-60 years) and V (61-80 years). Except for Group V, which comprised nine women and eight men, all groups were equally divided with respect to gender (20 M/20 F). Surface electromyographic records of masticatory muscles were obtained at rest and during maximal voluntary contraction, right and left laterality, maximal jaw protrusion and maximal clenching in the intercuspal position. Statistically significant differences (P < 0.05) were found in all clinical conditions among the different age groups. Considerably different patterns of muscle activation were found across ages, with greater electromyographic activity in children and youth, and decreasing from adults to aged people.

Correlation between bite force and electromyographic activity in dentate and partially edentulous individuals.

Dental absence interferes in the physiological functioning of the masticatory system, promoting occlusal and functional alterations. The purpose of this study was to verify maximal bite force and maximal bite force correlated with electromyographic activity in 14 partially edentulous and 14 dentate individuals. Bite force in right and left molar and incisor regions were registered using a dynamometer with capacity of up to 1000N, adapted for oral conditions and at the same time electromyography was performed using Myosystem-Br1 with electrodes positioned on right and left masseter and temporalis muscles, and one reference electrode on the frontal bone. The highest value out of three recordings was considered the individual's maximal bite force. Statistical analysis of the bite force data was performed by means of t-test and Pearson's bivariate correlation test was used for the analysis between bite and electromyographic activity using SPSS 12.0 software. Dentate individuals showed greater maximal bite force in the three regions. Correlations between electromyographic activity and bite force in the dentate group obtained positive coefficients for every muscle in the right molar region, for the left temporalis in the left molar region, and for every muscle in the incisive region. For the partially edentulous group, only the left temporalis muscle presented a positive correlation in the right molar region, there was positive correlation for the right masseter and right and left temporalis in the left molar region, and, in the incisive region, every muscle presented negative correlation. These data evidence the strong influence of dental loss over the maximal bite force and small correlation between bite force and electromyographic activity.

Anatomical variations of the mandibular nerve and its branches correlated to clinical situations.

AIM: The mandibular division of the trigeminal nerve is the largest of the three major divisions of the trigeminal nerve (fifth cranial nerve). In this way, many health professionals belonged to different fields are commonly evaluating patients suffering with orofacial pain and stomatognatic dysfunction associated to this structure. But, in the most cases, it is difficult to establish a correct diagnosis due to the anatomical complexity of the head and neck surfaces, especially when the focus is the trigeminal nerve. Thus, the objective of this research was to present the anatomical variations of the mandibular nerve and its branches correlated to more common clinical situations. METHODS: For this purpose, 20 human heads were anatomically dissected, so to study their structures, an external, medial and endocranial view. RESULTS: No significant variations related to ophthalmic and maxillary nerves were observed. Anatomical variations were observed in 20% of the total human heads dissected, all related to mandibular nerve and its branches: masseter, temporal, auriculotemporal and lingual. Variations in three to seven, on the number of the following nerves ramus, masseter and temporal were described. CONCLUSIONS: According to the present data using the described methodology, it was possible to conclude that anatomical variations are present in many subjects and they can explain many clinical situations that involve the stomatognathic structures.

Masticatory process in individuals with maxillary and mandibular osteoporosis: electromyographic analysis.

UNLABELLED: The masseter and temporal muscles of patients with maxillary and mandibular osteoporosis were submitted to electromyographic analysis and compared with a control group. In conclusion, individuals with osteoporosis did not show significantly lower masticatory cycle performance and efficiency compared to the control group during the proposal mastications. INTRODUCTION: This study aimed to examine electromyographically the masseter and temporal muscles of patients with maxillary and mandibular osteoporosis and compare these patients with control patients. METHODS: Sixty individuals of both genders with an average age of 53.0 +/- 5 years took part in the study, distributed in two groups with 30 individuals each: (1) individuals with osteoporosis; (2) control patients during the habitual and non-habitual mastication. The electromyographic apparel used was a Myosystem-BR1-DataHomins Technology Ltda., with five channels of acquisition and electrodes active differentials. Statistical analysis of the results was performed using SPSS version 15.0 (Chicago, IL, USA). RESULTS: The result of the Student's t test indicated no significant differences (p > 0.05) between the normalized values of the ensemble average obtained in masticatory cycles in both groups. CONCLUSION: Based on the results of this study, it was concluded that individuals with osteoporosis did not show significantly lower masticatory cycle performance and efficiency compared to control subjects during the habitual and non-habitual mastications. This result is very important because it demonstrates the functionality of the complex physiological process of mastication in individuals with osteoporosis at the bones that compose the face.

Evidence of muscle role over the cranio-facial skull development in Angle's Class III dental malocclusion under the clinical rest position.

OBJECTIVE: The purpose of this study was to investigate correlations between masseter muscle activity and cephalometric measures in Angle's class III dental malocclusion under the clinical rest position. METHODS: Eight volunteers, both sex, age 7-12, were submitted to an electromyographic analysis of the masseter muscle, subdivided in the upper, medium and lower regions, and to a cephalometric analysis of 15 angle and linear measurements. After normalization, the data was subjected to a statistical, non-parametric Spearman correlation test, by means of the SPSS 12.0 program. RESULTS: Statistically significant correlations were observed between the clinical rest condition before and after the execution of exercises (a serie of mandibular movements), and the cephalometric measurements angle SN GoGn, angle SNA, angle SNB, angle SND, upper incisive. NA angle, upper incisive-NA distance, lower incisive. NB angle, lower incisive-NB distance and angle IMPA, and the different regions of the masseter muscle. CONCLUSIONS: The significant statistical correlation found between the cephalometric analysis and the function of the masseter muscle indicate a strong influence of the musculature under a clinical rest condition over the cranial-facial development in the Angle's class III dental malocclusion. There is a selective activity of the different regions of the masseter muscle under the clinical rest condition in the Angle's class III dental malocclusion. There is a demand for research to complement these findings in other classes of dental malocclusion.

Effect of congenital blindness on EMG activity of the facial muscles.

The aim of this work was to analyze electromyographically the facial muscles: orbicularis oris (upper and lower fascicles), orbicularis oculi (right and left lateral portions) and frontal, in blind and clinically normal (control) individuals, in various clinical conditions. Electromyographic averages of all data collected were normalized by maximal voluntary contraction of the studied muscles and statistical analysis was performed by Student's t test, using "Statistical Package for the Social Sciences" software--SPSS 12.0 (Chicago, IL). It was found that electromyographic alterations occur in the facial musculature that influences facial expressions of individuals. Results for the orbicularis oris muscle demonstrated that myoelectric activity among blind and control subjects was greater for the control group at muscular rest, blowing, and in labial projection. Electromyographic analysis of the orbicularis oculi among blind and control subjects in three clinical conditions studied demonstrated that activity was greater for the control group at muscular rest, blinking, and forced blinking. For the frontal muscles were demonstrated that electromyographic activity was greater for blind individuals. These data suggest the influence of congenital blindness on muscular development, including alterations in electromyographic activity of skin musculature in individuals with visual impairment.

Electromyographic evaluation of the masseter and temporal muscles activity in volunteers submitted to acupuncture.

AIM: To assess the effect of acupuncture on the temporal and masseter muscles activity employing surface electromyography. METHODS: Thirty volunteers were evaluated according to three groups of acupuncture application: selected local points, selected points at distance and association of local and distant points. Bipolar surface electrodes were positioned bilaterally on the anterior portion of temporal muscle, as well as in the median region of masseter muscle. An electromyograph connected to a computer and a specific software registered the muscular activity before and after acupuncture, in the following experimental conditions: 1- Rest Position (RP); 2- Maximum Intercuspation Clenching (MIC); 3- Bilateral Molar Clenching with Cotton Rolls (BMCCR). The mean values obtained for the activities of the studied muscles were submitted to Analysis of Variance and Tukey complementary test. RESULTS: The electromyographic activity of the studied muscles was lower after the application of the methods of acupuncture in the Rest, and higher after the application of the acupuncture in the Maximum Intercuspation Clenching; the electromyographic activity of the temporal muscle was higher than the masseter muscle in the Rest and lower in the Bilateral molar clenching with cotton rolls; the electromyographic activity showed to be modified after the three used methods of acupuncture in the Maximum Intercuspation Clenching. In this clinical condition, the method of long-distance acupuncture was higher than the application of local needles and the associated method. CONCLUSION: Acupuncture provided alterations in the activity of the studied muscles, favoring conditions of rest and muscular tightness.

Electromyographical analysis of the masseter muscle in dentulous and partially toothless patients with temporomandibular joint disorders.

This study had as objective to analyze with computerized electromyography the masseter muscles bilaterally in twenty individuals with temporomandibular joint dysfunction (DTM), being: the group I consisting of ten individuals with complete dentition and group II constituted by ten individuals with posterior dental absences; comparing nine clinical activities: rest before and after exercises, maximum habitual intercuspation (MIH), right and left laterality, forced centric occlusion, protrusion, bilateral molar bite and chewing. It was utilized the Electromyography K6I-Myotronics, with eight canals and surface silver electrodes. By means of the results analysis, we verified significant differences between the groups (p < 0.01), being group I, presented greater electromyographic activity (32.98 microvolts), than the group II (22.31 microvolts), suggesting that this individuals presented low muscular activity. The interaction between the groups and the clinical activities was significant (p < 0.01), as well as between the groups, clinical activities and muscles (p < 0.05). To clarify which amongst the relative averages the clinical activities were different, calculated the critical value of Tukey, being that rest before and after exercises, MIH, right and left laterality, presented averages with similar distributions, with values below of the values of Tukey, as well as forced centric occlusion, protrusion, bilateral molar bite and chewing presented higher values than Tukey. Based on this research's data, we concluded that the electromyographical analysis of the masseter muscles in Individuals with TMD, dentulous and with dental absence showed that individuals with TMD, dentulous or not, presented elevated muscular activity in rest position and individuals with TMD, dentulous, presented higher electromyographical activity than the individuals with TMD and lacking posterior teeth.

Changes in masticatory muscles activity associated with Eagle's syndrome.

AIM: This study had the goal of analyzing masseter and temporalis muscles of individuals with Eagle's Syndrome, establishing comparisons with healthy control individuals by means of electromyography, due to the importance of the masticatory musculature upon the various functions of the stomatognathic system and the effects of Eagle's syndrome on this system's functioning. METHOD: Ten women with Eagle's syndrome and ten healthy controls volunteered to participate in the study. A Myosystem I system, Prosecon Ltda (Uberlândia/MG, Brazil) with 12 channels was used Active differential electrodes were placed on the belly of both masseters and on the anterior temporalis. Firstly, rest position was registered and then the patients were instructed to clench their teeth with maximum strength for four seconds to register the Clenching at Usual Maximum Intercuspation. Next, the clinical conditions of chewing, mandible laterality, and water swallowing were performed. RESULTS: The normalized data of muscle activity during the different clinical conditions and at rest position were compared by Student's t test, considering two groups: controls and syndrome bearers. The masticatory muscles of every syndrome bearers showed hyperactivity during the analyzed clinical conditions; except for chewing, in which the right masseter did not show greater electromyographic activity. CONCLUSION: It is concluded that individuals with the syndrome showed muscular hyperactivity when compared to healthy individuals, due to the interference of the elongated styloid process, which leads to dysfunctions of the stomatognathic system in the syndrome bearers, with signs and symptoms related to craniofacial pain, dysphagia, otalgy, temporomandibular dysfunctions, and headache.

The effect of the Schwartz-Jampel syndrome on masticatory and facial musculatures--an electromyographic analysis.

This study had the goal to perform an electromyography evaluation of the orbicularis oris, orbicularis oculi, masseter, and temporal muscles of two siblings with Schwartz-Jampel syndrome (SJS), in different clinical activities, comparing them to healthy controls (C). The Schwartz-Jampel syndrome is a rare genetic disorder (71 cases reported in worldwide literature), in which myotonia may be observed in the facial muscles, determining a standard facie that shows an appearance of someone who is sad and weeping For the electromyography (EMG), a Myotronics--K61 electromyographer, was used, with superficial and disposable silver chloride electrodes. By means of the analysis of the obtained results, we observed significant statistical differences for the masseter muscle and for orbicularis oculi muscles (p < 0.01) among the studied groups, in which the individuals with SJS presented greater muscular activity than the normal ones, used as healthy controls. The statistical difference between the two groups was not significant for the temporal muscle, as well as for the orbicularis oris muscle, although the EMG averages were much greater in patients who were bringers of the syndrome. This high muscular activity may be related to the facial osseous alterations, evidenced in both patients with SJS, such as the mandibular migrognathia, with an atrophy of the mandibular cortex and a consequent approximation between the inferior dental root apices and the mandible base, as well as the presence of hypoplastic condyles in terms of size and height.

Electromyographic analysis of the upper and lower fascicles of the orbicular oris muscle, in edentulous patients, before and after complete denture implantation.

The orbicular oris muscle, including its upper and lower fascicles, the alveolar processes and dental arches, constitute a complex morpho-functional system, located in the lower third of the face, which is very important for the clinical determination of the occlusal vertical dimension. Any alteration of this system, as in the case of edentulous patients, might produce esthetic problems and alterations in the muscular tonicity. This study was aimed to evaluate the electromyographic behaviour of that musculature in patients while pronouncing the syllables PAH, BAH, MAH, SAH, FAH, VAH, MEE, and the word MISSISSIPPI. Edentulous patients were submitted to electromyographic tests under two clinical conditions: before and after the implantation of complete dentures. A K6-I EMG Light Channel Surface Electromyograph was utilized (Myo-tronics Co. Seattle, WA, USA). The Analysis of Variance, with 3 sources of variation (Syllables, Muscles and Clinical Conditions) and 12 repetitions, indicated statistically significant differences in the pronunciation of the different syllables. For the Muscles factor, the analysis revealed higher electromyographic readings in the lower fascicle of the orbicular oris muscle, as compared with those of the upper fascicle. The comparison among the Clinical Conditions indicated higher electromyographic values for the edentulous condition (i.e., before complete denture implantation), as compared to those recorded after denture implantation.

Electromyographic study of the masseter muscle in individuals with Class II malocclusion.

The objective of this work was to study the action of the masseter muscle through electromyography and to analyze the difference of action potential generated by several pre-established jaw movements in individuals with Class II malocclusion. It was studied nine young individuals from both sexes, with ages ranging from 17 to 35, with no history of orthodontic treatment and/or having some kind of alteration in the temporomandibular joint (T.M.J.). In order to collect the electromyographic signs we have used a TECA TE-4 Electromygraph and surface electrodes type Beckman. The data were submitted to nonparametric statistical analysis by Friedman test. The response variables left occlusive contact, free protraction, protraction with occlusive contact, incisor mastication, right molar mastication and forced central occlusion movements showed significant outcomes. Thus we have concluded that as the occlusive balance suffers alterations, the malocclusions become predominant and the masseter muscle undergoes functional and structural modifications.

Tipo e freqüência de alteraçöes dentárias e periodontais em cäes na regiäo de Jaboticabal, SP / Type and frequency of dental diseases and disorders in dogs in the region of Jaboticabal, SP

Realizou-se levantamento das alteraçöes periodontais, dentárias e da oclusäo dental espontâneas em 215 cäes necropsiados ou que morreram por razöes diversas, no Hospital Veterinário da Faculdade de Ciências Agrárias e Veterinárias da UNESP, Jaboticabal. As alteraçöes periodontais, identificadas por exame clínico, foram tabuladas considerando raça, dieta, faixa etária e sexo. Os índices gengival e de placa foram aplicados a todos os animais e análise radiográfica foi realizada quando necessária. O levantamento epidemiológico revelou elevada freqüência de alteraçöes relacionadas à presença de placa bacteriana e de alteraçöes gengivais. Observou-se também agravamento das alteraçöes com o avançar da idade, evidenciado radiograficamente. Os animais alimentados somente com raçäo apresentaram menor freqüência de alteraçöes relacionadas aos índices gengival e de placa, em relaçäo àqueles alimentados com dieta caseira. Näo se observou diferença entre sexos quanto às alteraçöes dentárias

Scanning electron microscopy of the rat tongue mucosa with special attention to the bacteria on epithelial cell membranes.

The authors examined the filiform and fungiform papillae surfaces of rat tongue by scanning electron microscopy showing the numerous groupings of bacteria on the epithelial cell membranes. The fungiform papillae were round in shape and present few bacteria. The epithelial cell of filiform papillae revealed numerous streptococci. The grouping of the bacteria are attached on the epithelial cell membrane, demonstrating three-dimensional SEM images.

Tipo e freqüência de alterações dentárias e periodontais em cães na região de Jaboticabal, SP

The type and frequency of dental diseases and disorders in 215 dogs were studied. Periodontal disorders were identified through clinical investigation, microbiological and histopathological examination. Radiographs were taken in case of severe dental and periodontal disorders. Disorders were classified according to breed, diet, age and sex. Gingival and plaque indexes were estimated in all animals. The study showed a high frequency of disorders like dental plaque and gingival lesions. Disorders were more severe in older animals. Animals fed only with commercial rations showed a lower frequency of disorders related to gingival and plaque than animals fed with human waste food. No differences between male and female frequency of disorders were observed. The radiographic evaluation was an important and useful method for the diagnosis of periodontal disorders.

Realizou-se levantamento das alterações periodontais, dentárias e da oclusão dental espontâneas em 215 cães necropsiados ou que morreram por razões diversas, no Hospital Veterinário da Faculdade de Ciências Agrárias e Veterinárias da UNESP, Jaboticabal. As alterações periodontais, identificadas por exame clínico, foram tabuladas considerando raça, dieta, faixa etária e sexo. Os índices gengival e de placa foram aplicados a todos os animais e análise radiográfica foi realizada quando necessária. O levantamento epidemiológico revelou elevada freqüência de alterações relacionadas à presença de placa bacteriana e de alterações gengivais. Observou-se também agravamento das alterações com o avançar da idade, evidenciado radiograficamente. Os animais alimentados somente com ração apresentaram menor freqüência de alterações relacionadas aos índices gengival e de placa, em relação àqueles alimentados com dieta caseira. Não se observou diferença entre sexos quanto às alterações dentárias.

Diameter of axons in the lingual nerve of the mouse. A preliminary investigation.

The lingual nerves from ten mice were examined so that normal axonal populations could be determined. After perfusion fixation, they were removed and processed, and sections were taken from nerves for transmission electron microscopy. The fiber-diameter spectrum of unmyelinated fibers for the mouse lingual nerve is characterized by a unimodal curve with the more pronounced peak in the medium-diameter fiber range (0.28 micron). The spectrum from the myelinated fibers also shows a population of axons of the lingual nerve (0.22-3.2 microns) that reflects different functional specializations. These data establish some baseline values for morphological evaluation of the effects of experimental lingual nerve damage.

Light and scanning electron microscopic study of the digestive tract in the catfish Pseudoplatystoma Corruscans Agassiz, 1829 (Pisces, Pimelodidae)

O trato digestivo do surubin Pseudoplatystoma corruscans foi estudado morfologicamente utilizando a microscopia de luz e eletrônica de varredura (MEV), além de técnicas histoquímicas. O epitélio estratificado do palato e faringe está constituído de uma camada basal com células indiferenciadas de núcleos elípticos de posiçäo central; uma camada média constituída de células intermediárias indiferenciadas, células gigantes acidófilas e células mucosas; e uma camada superficial de células achatadas. O esôfago apresenta estrutura semelhante, com células gigantes acidóficas menos numerosas e células poilimorfonucleares entre as células da camada basal, ao MEV, o palato evidenciou vários sulcos, sendo que a porçäo superficial está constituída de células hexagonais ou poligonais, em cuja área apical está presente as microcristas. A parte superficial da faringe apresenta células com microcristas dispostas concentricamente. O esôfago mostrou pregas longitudinais complexas, um epitélio estratificado cujas células apresentam microcristas irregulares. Os carbohidratos presentes nas células do tracto digestivo foram identificados como sendo mucosubstâncias neutras e ácidas

Morphological observations of the sensory nerve endings of the Calomys callosus' lingual mucosa, employing the silver impregnation method

Estudaram-se as características morfológicas das terminaçöes nervosas sensitivas da mucosa lingual de Calomys callosus, empregando-se a técnica de impregnaçäo pela prata. Para a obtençäo das peças, cada animal foi anestesiado com injeçäo intraperitonial de tionembutal sódico. As peças foram fixadas em soluçäo de formalina a 10 por cento por um período de 20 dias à temperatura ambiente e cortes espessos de 40-60 micrometros foram obtidos em criostato Linde. As preparaçöes foram coradas pela técnica de impregnaçäo pela prata. De acordo com os nossos resultados podemos concluir que: 1) todas as regiöes (anterior, média e posterior) da mucosa dorsal da língua de Calomys callosus possuem terminaçöes nervosas sensitivas; 2) as terminaçöes nervosas podem ser simples ou complexas localizando-se no tecido conjuntivo da lâmina própria ou no interior das papilas conjuntivas das papilas filiformes, fungiformes e valadas, 3) as terminaçöes nervosas livres no interior das papilas fungiformes dispöem-se longitudinalmente ou formando uma rede complexa de fibras nervosas terminais