RESUMEN
This clinical and laboratory study evaluated the effect of two methods of disinfection in different modes at the volume changes of alginate dental impressions and plaster models poured from them, as compared to the same parameters of plastic master models (PMM), using three-dimensional non-contact laser scanner and software. Immersion chemical disinfection for 15 min, microwave disinfection at 354 W for 10 minutes and combined disinfection with the power of 319 W for 4 minutes did not significantly affect the volumetric dimensional accuracy of the alginate impressions (P > 0.05).
Asunto(s)
Alginatos/química , Materiales de Impresión Dental/química , Prótesis Dental/métodos , Desinfectantes/química , Desinfección/métodos , Técnica de Impresión Dental , Humanos , Procesamiento de Imagen Asistido por Computador/estadística & datos numéricos , Imagenología Tridimensional , Ensayo de Materiales , Microondas , Propiedades de SuperficieRESUMEN
The lysate and recombinant antigens of various production included informula of ELISA-test-systems were analyzed. The ELISA-test-systems are used for detection of IgG to Herpes simplex virus type I and II. For testing the panel of serums PTH 201 (BBI Inc.) were used. The samples of this panel contain antibodies to Herpes simplex virus type I and II in mixed titers. The 69 serums of donors were used too (17 samples had IgG to Herpes simplex virus type I, 23 samples to Herpes simplex virus type II and 29 samples had no antibodies to Herpes simplex virus). The diagnostic capacity of mixture of recombinant antigens gG1 Herpes simplex virus type I and gG2 Herpes simplex virus type II (The research-and-production complex "DiaprofMed") was comparable with mixture of lysate antigen Herpes simplex virus type I and II (Membrane) EIE Antigen ("Virion Ltd."). In the test-systems for differentiation of IgG to Herpes simplex virus type I the recombinant antigen gG1 Herpes simplex virus type I proved to be comparable with commercial analogue Herpes simplex virus-1 gG1M ("Viral Therapeutics Inc."'). At the same time, capacity to detect IgG to Herpes simplex virus type II in recombinant protein gG2 Herpes simplex virus type II is significantly higher than in its analogue Herpes simplex virus-2 gG2c ("Viral Therapeutics Inc.").
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/métodos , Herpes Simple/diagnóstico , Herpesvirus Humano 1/aislamiento & purificación , Herpesvirus Humano 2/aislamiento & purificación , Antígenos Virales/sangre , Antígenos Virales/inmunología , Antígenos Virales/aislamiento & purificación , Herpes Simple/inmunología , Herpes Simple/virología , Herpesvirus Humano 1/patogenicidad , Herpesvirus Humano 2/inmunología , Herpesvirus Humano 2/patogenicidad , Humanos , Inmunoglobulina G/sangre , Proteínas Recombinantes/sangreRESUMEN
In the review it has been presented the conceptional analysis of modern state of stem cells use problem (SC) in gene therapy ex vivo. Principles of vectorial systems construction have been expounded, designed for SC transfection. It has been displayed results of experimental investigations and clinical data on transplantation of genetically modifiable SC at different monogenous and multifactorial diseases. Special attention is focused on the problem of biosecurity ensuring.
Asunto(s)
Ingeniería Genética , Terapia Genética , Vectores Genéticos , Trasplante de Células Madre , Células Madre , Animales , Humanos , Células Madre/metabolismo , Células Madre/fisiologíaRESUMEN
Imunophenotic characteristic of the mesenchymal stem and progenitor cells, their tissue origin and functional peculiarities as well as immunological aspects of its transplantation are presented in the article. Also the address migration and the site-specific differentiation of mesenchymal stem cells its age-related and pathological process induced changes are discussed. Prospects of clinical use of mesenchymal stem cells for the treatment of degenerative arthritis, spinal cord injuries, myocardial infarction and other disorders are shown.