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The quantity and quality of dissolved organic matter (DOM) exported from source areas are closely related to hydrological linkage between source areas and streams, that is hydrological connectivity. However, understanding of how hydrological connectivity regulates the export of catchment DOM components remains inadequate. In this study, high-frequency monitoring of groundwater and runoff from subtropical humid catchment was conducted for 20 months, and hydrological connectivity was quantitatively characterized by considering both surface and subsurface hydrological processes. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was utilized to investigate the DOM molecular composition. Results showed that over half of the areas in the catchment could not persistently establish hydrological connectivity with the stream during the rainfall. The average proportion of lignin was the highest in DOM components, followed by tannin and proteins. Additionally, both modified aromaticity index and double bond equivalence reached maximums at peak discharge, reflecting terrestrial materials could increase DOM aromaticity and unsaturated degree. Partial least square-structural equation modeling revealed significantly direct effects of rainfall, antecedent conditions, and hydrological connectivity on dissolved organic carbon (DOC) export. Furthermore, nonlinear relationships were observed between hydrological connectivity and DOC, tannin, and condensed aromatics. Specifically, the instantaneous DOC flux increased dramatically when the hydrological connectivity strength exceeded 0.14; tannin and condensed aromatics exhibited a rapid increase with rising connectivity strength, but remained stable at connectivity strength above 0.25. However, hydrological connectivity showed no significant correlation with unstable components (such as lipids, protein, amino sugars, and carbohydrates). These results provide new insights into hydrological controls on the quantity and quality of DOM export and contribute to developing appropriate catchment management strategies for carbon storage.
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Agua Subterránea , Hidrología , Agua Subterránea/química , Ríos/química , Monitoreo del Ambiente , Taninos/análisis , Compuestos Orgánicos/análisis , LluviaRESUMEN
Subsurface runoff represents the main pathway of nitrate transport in hilly catchments. The magnitude of nitrate export from a source area is closely related to subsurface hydrological connectivity, which refers to the linkage of separate regions of a catchment via subsurface runoff. However, understanding of how subsurface hydrological connectivity regulates catchment nitrate export remains insufficient. This study conducted high-frequency monitoring of shallow groundwater in a hilly catchment over 17 months. Subsurface hydrological connectivity of the catchment over 38 rainfall events was analyzed by combining topography-based upscaling of shallow groundwater and graph theory. Moreover, cross-correlation analysis was used to evaluate the time-series similarity between subsurface hydrological connectivity and nitrate flux during rainfall events. The results showed that the maximum subsurface hydrological connectivity during 32 out of 38 rainfall events was below 0.5. Although subsurface flow paths (i.e., the pathways of lateral subsurface runoff) exhibited clear dynamic extension and contraction during rainfall events, most areas in the catchment did not establish subsurface hydrological connectivity with the stream. The primary pattern of nitrate export was flushing (44.7%), followed by dilution (34.2%), and chemostatic behavior (21.1%). A threshold relationship between subsurface hydrological connectivity and nitrate flux was identified, with nitrate flux rapidly increasing after the subsurface connectivity strength exceeded 0.121. Moreover, the median value of cross-correlation coefficients reached 0.67, which indicated subsurface hydrological connectivity exerts a strong control on nitrate flux. However, this control effect is not constant and it increases with rainfall amount and intensity as a power function. The results of this study provide comprehensive insights into the subsurface hydrological control of catchment nitrate export.
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Agua Subterránea , Nitratos , Nitratos/análisis , Movimientos del Agua , Ríos , HidrologíaRESUMEN
Anthropogenic activities alter the underlying surface conditions and arrangements of landscape features in a drainage basin, interfering with the pollutant (e.g., dissolved nitrogen, phosphorus) transport network configuration and altering the hydrological response. Assessing the impact of anthropogenic activities on hydrological connectivity for natural-artiï¬cial catchment is critical to understand the hydrological-driven ecosystem processes, services and biodiversity. However, quantifying this impact at catchment scale remains challenging. In this study, a new framework was proposed to quantify the impact of anthropogenic activities on hydrological connectivity combined with graph theory and network analysis. This framework was exemplified in a natural-artiï¬cial catchment of the Yangtze River basin of China. Based on remote sensing and field-investigated data, three transport networks were constructed, including natural transport network (N1), ditch-road transport network (N2), and terrace-dominated transport network (N3), which reflected the different human intervention. The results showed that human intervention improved the connectivity of the nodes and enhanced the complexity of the catchment transport network structure. Anthropogenic activities significantly decreased the hydrological structural connectivity of the catchment. In particular, compared with the N1 network, the critical nodes for hydrological connectivity which were judged by connectivity indexes were reduced by 92.94% and 95.29% in the N2 and N3 network, respectively. Furthermore, the ditch-road construction had a greater impact than terraces in decreasing hydrological structural connectivity at catchment scale. This framework has proven effective in quantifying the hydrological connectivity analysis under different human intervention at the catchment scale and facilitates the improvement of catchment management strategies.
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As a recognized rare and highly fatal disease, hereditary angioedema (HAE) is difficult to diagnose and characterized by recurrent edema involving the head, limbs, genitals and larynx, etc. Diagnosis of HAE is not difficult. However, low incidence and lack of clinical characteristics lead to difficulty of doctors on timely diagnosis and correct intervention for HAE patients. Therefore, it is crucial to improve the awareness of this disease and prevent its recurrence. for HAE patients. In view of absent cognition of doctors and the general public on HAE, patients often suffer from sudden death or become disabled due to laryngeal edema which cannot be treated in time. Thus, based on the Internet mobile terminal platform, the team set up an all-day rapid emergency response system which is provided for HAE patients by setting up "one-click help". The aim is to offer optimization on overall management of HAE and designed the intelligent follow-up management to provide timely assistance and specialized suggestion for patients with acute attacks.
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Angioedemas Hereditarios , Humanos , Angioedemas Hereditarios/terapia , Angioedemas Hereditarios/tratamiento farmacológicoRESUMEN
Allergic diseases affect about 40% of the world's population. Environmental factors are important in the occurrence and development of allergic diseases. Dust mites are one of the most important allergens in the indoor environment. The World Health Organization proposes the "four-in-one, combination of prevention and treatment" treatment principle for allergic diseases, in which environmental control to avoid or reduce allergens is the first choice for treatment. Modern people spend much more time at home (including sleeping) than outdoors, and the control of the home environment is particularly critical. This practice introduces the hypoallergenic home visit program, which including home environment assessment, environmental and behavioral intervention guidance, and common household hypoallergenic supplies and service guidance for the patient's home environment. The real-time semi-quantitative testing of dust mite allergens, qualitative assessments of other indoor allergens, record of patients' household items and lifestyle, and precise, individualized patient prevention and control education will be conducted. The hypoallergenic home visit program improves the doctors' diagnosis and treatment data dimension, and becomes a patient management tool for doctors outside the hospital. It also helps patients continue to scientifically avoid allergens and irritants in the environment, effectively build a hypoallergenic home environment, reduce exposure to allergens in the home environment, and achieve the goal of combining the prevention and treatment of allergic diseases.
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Hospitales , Estilo de Vida , Humanos , SueñoRESUMEN
The dissolved carbon concentration, which is responsible for aquatic ecosystem productivity and water quality, is tightly coupled with hydrological processes. Excess dissolved carbon may exacerbate eutrophication and hypoxia in aquatic ecosystems and lead to deterioration of water quality. Storm events dominate the dynamics of dissolved carbon concentrations, and this nonlinear behavior exhibits significant time scale dependence. Here, we identified inter- and intra-event variability in the dissolved carbon concentration-discharge (C-Q) relationship in an agriculture-intensive catchment. The driving factors of C-Q hysteresis patterns for dissolved inorganic carbon (DIC) and organic carbon (DOC) were quantified by redundancy analysis combined with hierarchical partitioning. At the inter-event scale, DIC exhibited mainly clockwise hysteresis, indicating an exhaustible, proximal source (e.g., groundwater). However, DOC hysteresis was generally counter-clockwise, indicating distal and plentiful sources (e.g., soil water) in the agricultural catchment. Hierarchical partitioning showed that total rainfall, peak discharge and flood intensity explained 28.38% of the total variation in C-Q hysteresis for DIC and 39.87% for DOC at the inter-event scale. At the intra-event scale, time series analysis of dissolved carbon concentration and discharge indicated the interconversion of supply limitation to transport limitation, which depends on the activation of the specific DIC or DOC source zones. These findings provide significant insights into understanding the dynamics of dissolved carbon during storm periods and are important for targeted watershed management practices aimed at reducing carbon loading to surface waters.
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Carbono , Ríos , Carbono/análisis , Ecosistema , Ciclo del Carbono , Monitoreo del AmbienteRESUMEN
Neonicotinoids pollution poses a serious threat to aquatic ecosystems. However, there is currently little knowledge about how neonicotinoids are transferred from the agricultural environment to the aquatic environment. Here, we conducted in situ high-frequency monitoring of neonicotinoids in soil-water systems along the hydrological flow path during rainfall to explore the horizontal and vertical transport mechanisms of neonicotinoids. The collected samples included 240 surface runoff, 128 subsurface runoff, 60 eroded sediment, 120 soil and 144 soil solution, which were used to analyse neonicotinoids concentrations. Surface runoff, subsurface runoff and eroded sediment were the three main paths for the horizontal migration of neonicotinoids. In the CK (citrus orchards without grass cover) and grass-covered citrus orchards, there are 15.89% and 2.29% of the applied neonicotinoids were transported with surface runoff, respectively. While in the CK and grass-covered citrus orchards, there are only 1.23% and 0.19% of the applied neonicotinoids were transported with eroded sediment and subsurface runoff. Although the amount of neonicotinoids lost along with eroded sediment was small, the concentration of neonicotinoids in eroded sediment was two orders of magnitude higher than the concentration of neonicotinoids in sediments of the surface water. Meanwhile, neonicotinoids migrated vertically in soil due to water infiltration. In the CK and grass-covered citrus orchards, there are 57.64% and 24.36% of the applied neonicotinoids were retained in soil and soil solution, respectively, and their concentration decreased as soil depth increased. Another noteworthy phenomenon is that more neonicotinoids migrated to deeper soil layers under grass cover compared with no grass cover because grass roots promoted the formation of cracks and vertical preferential flow. Our results are expected to improve the accuracy of neonicotinoids pollution prediction by considering migration paths, including surface and subsurface runoff and eroded sediment.
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Suelo , Agua , Ecosistema , Monitoreo del Ambiente , Neonicotinoides , Lluvia , Movimientos del AguaRESUMEN
Objective: To investigate the Helicobacter pylori (H. pylori) eradication rate and improvement of dyspepsia in patients who were newly diagnosed with H. pylori infection and dyspepsia and treated by bismuth-containing quadruple therapy followed by Jing-Hua-Wei-Kang(JHWK). Methods: Patients who were newly diagnosed with dyspepsia and H. pylori infection and treated in 16 medical centers in China between December 1, 2017 and September 30, 2019 were randomly divided into two groups. The experimental group received bismuth-containing quadruple therapy (esomeprazole+amoxicillin+furazolidone+colloidal bismuth pectin capsule, 14 days), followed by JHWK (30 days), and the course of treatment was 44 days in total. In the control group, the administration regimen was bismuth-containing quadruple therapy (esomeprazole+amoxicillin+furazolidone+colloidal bismuth pectin capsule, 14 days). The main outcome measure was H. pylori eradication rate, while the secondary outcome measures were dyspepsia symptom changes and adverse events during the treatment and the 1st month after treatment. Results: A total of 1 054 patients were included in the study. There were 522 cases enrolled in the experimental group, including 224(42.91%) men and 298(57.09%) women, and the age was 53(26, 73) years old; 532 cases enrolled in the control group, including 221(41.54%) men and 311(58.46%) women, and the age was 46(22, 71) years old. Based on PP analysis, it was found that the H. pylori eradication rate in the experimental group was significantly higher than those in the control group (93.85% vs 87.88%, P=0.001). In the group of all enrolled patients, the symptom dyspepsia after H. pylori eradication was significantly improved compared with that before treatment [4(4, 7) vs 15(10, 22), P<0.001], so was the superior and middle abdominal pain [1(1, 4) vs 4(1, 8), P<0.001], the postprandial fullness [1(1, 4) vs 4(4, 9), P<0.001], the early satiety [1(1, 1) vs 4(1, 4), P<0.001], and the heartburn [1(1, 1) vs 1(1, 4), P<0.001]. The symptom dyspepsia after treatment was significantly improved compared with that before treatment in the experimental, the control groups, the successful and the unsuccessful H. pylori eradication groups. The superior and middle abdominal pain after treatment was signifcantly improved than that before treatment [1(1, 2) vs 1(1, 4), P<0.001], so were the postprandial fullness [1(1, 3) vs 1(1, 4), P=0.002] and the dyspepsia[4(4, 7) VS 7(4, 10), P<0.001]. There was no statistically significant difference in the incidence of adverse events between the experimental group and the control group (1.34% vs 0.38%, P=0.09). Conclusions: Compared with bismuth-containing quadruple therapy, bismuth-containing quadruple therapy followed by JHWK significantly improves the H. pylori eradication rate without increasing the incidence of adverse events. H. pylori eradication therapy can improve symptoms of patients with H. pylori infection and dyspepsia.
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Dispepsia , Infecciones por Helicobacter , Helicobacter pylori , Amoxicilina/uso terapéutico , Antibacterianos/uso terapéutico , Bismuto/uso terapéutico , China , Quimioterapia Combinada , Dispepsia/tratamiento farmacológico , Femenino , Infecciones por Helicobacter/tratamiento farmacológico , Humanos , Masculino , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
Reservoir bays, which are affected by the reservoir and watershed characteristics, are the initial and most sensitive areas in the evolution process of reservoir water quality. However, the relationship between the watershed characteristics and nitrogen and phosphorus concentrations in reservoir bays is poorly understood. We selected 66 bays from the Danjiangkou Reservoir and sampled twice per year (storage and discharge periods) from 2015 to 2018 to monitor the total nitrogen (TN) and total phosphorus (TP) concentration in the waterbodies of the reservoir bays. Four types of watershed characteristic indices (topographic variables, soil variables, land-use composition, and landscape patterns) around these bays were obtained. We quantified the relationship between the TN and TP concentrations and watershed characteristics in the waterbodies of the reservoir bays using partial least squares regression (PLSR). The results showed that the mean concentrations of TN and TP in the storage period (TN:1.69 mg·L-1, TP:0.088 mg·L-1) were higher than those in the discharge period (TN:1.22 mg·L-1, TP:0.063 mg·L-1). The optimal PLSR models explained 67.9% and 82.5% of the TN concentration variability, and 65.4% and 67.2% of the TP concentration variability during the storage and discharge period, respectively. Based on the variable importance in the projection (VIP) values, soil erodibility had significant effects on the TN and TP concentrations. The key factors affecting the TN concentration were the slope gradient, basin relief, topographic wetness index, forest and agricultural land use, whereas the factors controlling the TP concentration were the landscape shape index, edge density, Shannon's diversity index and grass land use, although the TP concentration was also controlled by the patch density and contagion during the storage period, and by mean patch size and largest patch index during the discharge period. This study provides critical insights into sustainable landscape planning and effective reservoir water quality management.
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Whether channel erosion or topsoil erosion constitutes the dominant erosion process throughout in the hilly region of the Chinese Loess Plateau (CLP), which suffers perhaps the most severe soil erosion in the world, had been controversial for a long time. The present article attempts to use the mid-infrared (MIR) spectroscopy fingerprinting method to trace sediment sources within nine small catchments in the hilly region of the CLP. Two major categories of sediment sources are identified: channel sediment and topsoil. Sediments trapped by check dams are used as the final sediment transferred by soil erosion. Discriminant analysis shows that MIR spectroscopy can differentiate between the two kinds of source sediments very well. The contributions of channel sediment and topsoil to the total final sediment are quantified using partial least squares regression (PLSR) analyses of MIR spectra to compare the trapped sediment samples with experimental models. The results of the root mean square error of calibration, root mean square error of validation and coefficient of determination for 18 models all show that the MIR-PLSR models boast very high prediction abilities in the nine catchments. A comparison between the geochemical fingerprinting method and the MIR spectroscopy method in one catchment reveals that although the two methods agree well on the channel sediment contributions, the two methods produce a significant difference (R2â¯=â¯0.4). Overall, the MIR-PLSR results show that channel sediments contribute 19% to 66% of the total sediment with an average of 33⯱â¯16% in the nine small catchments. Our results indicate that although channel bank sediment is important, topsoil erosion is the predominant process in small dam-controlled catchments on the CLP. Furthermore, the MIR spectroscopy fingerprinting method can provide a useful, non-destructive, rapid and inexpensive tool for tracing sediment sources from different kinds of loess.
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Objective: To compare the outcomes of gastrectomy with either wedge resection of the pancreas or pancreaticosplenectomy for adenocarcinoma of the esophagogastric junction (AEG) invading pancreas. Methods: From May 2005 to December 2015, a total of 64 patients with AEG invading pancreas underwent gastrectomy with either wedge resection of pancreas (n=25) or pancreaticosplenectomy (n=39) at Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University. There were 53 males and 11 females, with a mean age of 60.2 years (range: 39 to 77 years). According to the AJCC esophageal cancer staging system, 8(th) edition, there were 27 patients in phase T4N0M0, 18 in phase T4bN1M0, 9 in phase T4bN2M0 and 10 in phase T4bN3M0. Follow-up was carried out every 6 months. The t-test for the measurement data and the χ(2) test, Fisher exact test or Wilcoxon ran-sum test for the enumeration data were used between the two groups. Survival curves were generated using the Kaplan-Meier method, and compared using the Log-rank test. Multivariate analysis was undertaken using the Cox proportional hazard model (forward stepwise regression). Results: In 39 patients who underwent pancreaticosplenectomy, incision infection occurred in 5 patients, anastomotic leak, peritoneal infection, lung infarction each occurred in 1 patient. There was 1 respiratory failure and 1 peritoneal infection in 25 patients undergoing wedge resection of the pancreas. There were no significant difference in the incidence of postoperative complications between the 2 groups (8/39 vs. 2/25, P=0.292), and no postoperative death in the study. Fifty-seven patients were followed up, with a follow-up rate of 89.1%. The 5-year overall survival rate was 32.3% in patients who underwent simultaneous gastrectomy and pancreaticosplenectomy, compared to 0 in those who underwent gastrectomy and wedge resection of the pancreas (χ(2)=4.484, P=0.034). The 5-year overall survival rate for patients who undergoing adjuvant chemotherapy was 32.3%, compared to 17.2% in whom underwent surgery alone (χ(2)=4.186, P=0.041). Conclusions: Survival benefit from R0 resection by simultaneous gastrectomy and pancreaticosplenectomy for AEG invading the pancreas can be achieved. Adjuvant chemotherapy is necessary for these patients.
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Adenocarcinoma/cirugía , Unión Esofagogástrica/cirugía , Gastrectomía , Neoplasias Pancreáticas/cirugía , Neoplasias Gástricas/cirugía , Adenocarcinoma/tratamiento farmacológico , Adenocarcinoma/mortalidad , Adulto , Anciano , Antineoplásicos/uso terapéutico , Quimioterapia Adyuvante , Femenino , Humanos , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Estadificación de Neoplasias , Páncreas/cirugía , Pancreatectomía , Neoplasias Pancreáticas/patología , Bazo/cirugía , Esplenectomía , Estómago/cirugía , Neoplasias Gástricas/tratamiento farmacológico , Neoplasias Gástricas/mortalidad , Análisis de SupervivenciaRESUMEN
This paper aims to investigate the hydrological response of a large-scale (8973â¯km2) mountainous watershed to different rainstorm spatial patterns and reforestation. Based on 32â¯years of observations, measurements of 184 rainstorm events and 125 sediment-producing events with complete hydrographs were analyzed. The K-means clustering method was used to classify the spatial patterns of rainstorm events in accordance with their event-based spatial rainfall characteristics. The 184 rainstorm events were classified into four spatial patterns, among which the spatial features differ significantly: (I) Spatial Pattern I (SPI) includes rainstorms with a low amount of cumulative areal rainfall (27.4â¯mm), the highest spatial variability (0.986), and the highest frequency; (II) Spatial Pattern II (SPII) includes rainstorms of high spatial variability (0.759) and the largest amount of local maximum daily rainfall (106.8â¯mm); (III) Spatial Pattern III (SPIII) includes rainstorms with a medium amount of cumulative areal rainfall (58.7â¯mm) and low spatial variability (0.362); and (IV) Spatial Pattern IV (SPIV) includes rainstorms with the largest amount of cumulative areal rainfall (117.2â¯mm) and the lowest spatial variability (0.313). Vegetation cover in the upper Du watershed was significantly improved after the implementation of the Grain-for-Green project. The average area-specific sediment yields (SSY) for the four SPs were 15.4, 65.5, 55.8, and 286.2â¯tâ¯km-2 before reforestation and decreased to 6.0, 59.3, 43.7 and 89.9â¯tâ¯km-2, respectively, after reforestation. ANOVA (analysis of variance) indicated that reforestation resulted in a significant reduction in runoff coefficient under SPIII and SPIV and a significant reduction in SSY under SPI and SPIV. A hysteresis analysis suggested that the proportion of events with a clockwise loop increased from 64.9% before reforestation to 82.1% after reforestation and that complex loops became less common during 2000-2010 under SPIV, thereby implying a reduced sediment supply.
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OBJECTIVE: Glioma is the most common form of brain tumor, accounting for over 50% of all primary tumors. Despite progress in the treatment of glioma, the prognosis is still poor. In this study, we examined protein-tyrosine phosphatase H1 (PTPH1) in human gliomas. MATERIALS AND METHODS: Cell growth potential was measured by CCK-8 assay and colony formation. Cell cycle distribution was measured by flow cytometry. Transwell assay was used to detect the motility of tumor cells. Real-time PCR and Western blot were used to measure the mRNA and protein expression of indicated genes. Xenograft model was established to measure the role of PTPH1 in vivo. RESULTS: The expression of PTPH1 was significantly higher in the tumor tissues as compared with that in the adjacent normal tissues. Knockdown of PTPH1 significantly slowed cell proliferation and reduced colony formation abilities in glioma cell lines U87 and U251. Additionally, knockdown of PTPH1 caused cell cycle arrest in the S-phase. Furthermore, depletion of PTPH1 in glioma U87 cells significantly limited tumor growth in a xenograft model. Interestingly, knockdown of PTPH1 also decreased cell migration abilities in both U87 and U251 cells. Accordingly, matrix metalloproteinase 9 (MMP9) was also decreased upon knockdown of PTPH1 in both cell lines. Moreover, we found that phosphorylated MEK (p-MEK) and phosphorylated MAPK (p-MAPK) were both decreased, whereas the total levels of MEK and MAPK remained unchanged after depletion of PTPH1 in both cell lines. CONCLUSIONS: Our data suggest that PTPH1 may be a novel biomarker that indicates the aggressiveness of gliomas. Targeting PTPH1 might be a promising strategy for the treatment of gliomas.
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Neoplasias Encefálicas/genética , Línea Celular Tumoral , Proliferación Celular/genética , Glioma/genética , Proteína Tirosina Fosfatasa no Receptora Tipo 3 , Biomarcadores , Humanos , Metástasis de la Neoplasia , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Disruption of neuronal iron homeostasis and oxidative stress are closely related to the pathogenesis of Parkinson's disease (PD). Ginkgetin, a natural biflavonoid isolated from leaves of Ginkgo biloba L, has many known effects, including anti-inflammatory, anti-influenza virus, and anti-fungal activities, but its underlying mechanism of the neuroprotective effects in PD remains unclear. The present study utilized PD models induced by 1-methyl-4-phenylpyridinium (MPP(+)) and 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) to explore the neuroprotective ability of ginkgetin in vivo and in vitro. Our results showed that ginkgetin could provide significant protection from MPP(+)-induced cell damage in vitro by decreasing the levels of intracellular reactive oxygen species and maintaining mitochondrial membrane potential. Meanwhile, ginkgetin dramatically inhibited cell apoptosis induced by MPP+ through the caspase-3 and Bcl2/Bax pathway. Moreover, ginkgetin significantly improved sensorimotor coordination in a mouse PD model induced by MPTP by dramatically inhibiting the decrease of tyrosine hydroxylase expression in the substantia nigra and superoxide dismutase activity in the striatum. Interestingly, ginkgetin could strongly chelate ferrous ion and thereby inhibit the increase of the intracellular labile iron pool through downregulating L-ferritin and upregulating transferrin receptor 1. These results indicate that the neuroprotective mechanism of ginkgetin against neurological injury induced by MPTP occurs via regulating iron homeostasis. Therefore, ginkgetin may provide neuroprotective therapy for PD and iron metabolism disorder related diseases.
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Biflavonoides/química , Hierro/química , Fármacos Neuroprotectores/química , Enfermedad de Parkinson/tratamiento farmacológico , 1-Metil-4-fenil-1,2,3,6-Tetrahidropiridina/efectos adversos , 1-Metil-4-fenilpiridinio/efectos adversos , Animales , Antígenos CD/metabolismo , Apoferritinas/metabolismo , Apoptosis , Caspasa 3/metabolismo , Línea Celular Tumoral , Supervivencia Celular , Modelos Animales de Enfermedad , Regulación Enzimológica de la Expresión Génica , Ginkgo biloba , Homeostasis , Humanos , Quelantes del Hierro/efectos adversos , Masculino , Potencial de la Membrana Mitocondrial , Ratones , Ratones Endogámicos C57BL , Neuronas/metabolismo , Estrés Oxidativo , Especies Reactivas de Oxígeno/metabolismo , Receptores de Transferrina/metabolismo , Superóxido Dismutasa/metabolismo , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
Nucleotide-binding oligomerization domain-containing protein-1 (NOD1) is a cytoplasmic pattern recognition receptor (PRR) and a key member of the NOD-like receptor (NLR) family. It has been reported that NLRs recognize a variety of microbial infections to induce the host innate immune response via modulation of NF-κB signaling. However, no reports on chicken NOD1 have been reported to date. In the current study, the full-length cDNA sequence of NOD1 was cloned. The complete open reading frame of NOD1 contains 2856 bp and encodes a 951 amino acid protein. Structurally, it is comprised of one caspase recruitment domain at the N-terminus, seven leucine-rich repeat regions at the C-terminus, and one NACHT domain between the N and C-termini. Phylogenetic analyses showed that chicken NOD1 clusters with duck and turkey. Furthermore, tissue-specific expression analyses of chicken NOD1 were performed using quantitative reverse transcription-PCR. NOD1 is widely distributed in various tissues, with the highest expression observed in testes. Finally, induced expression of chNOD1 and its associated adaptor molecule receptor-interacting protein 2, as well as the effector molecule NF-κB, was observed following S. enterica serovar Enteritidis infection. These findings highlight the important role of chicken NOD1 in response to pathogenic invasion. The present study is the first report of the cloning, expression, and functional analysis of chicken NOD1 and provides the foundation for future research on the structure and function of chicken NOD1.
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Proteínas Aviares/genética , Pollos/genética , Perfilación de la Expresión Génica , Proteína Adaptadora de Señalización NOD1/genética , Salmonelosis Animal/genética , Animales , Pollos/microbiología , Clonación Molecular , ADN Complementario/química , ADN Complementario/genética , Femenino , Interacciones Huésped-Patógeno , Masculino , Datos de Secuencia Molecular , FN-kappa B/genética , Proteína Adaptadora de Señalización NOD1/clasificación , Filogenia , ARN Mensajero/genética , ARN Mensajero/metabolismo , Proteína Serina-Treonina Quinasa 2 de Interacción con Receptor/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Salmonelosis Animal/microbiología , Salmonella enteritidis/fisiología , Análisis de Secuencia de ADNRESUMEN
INTRODUCTION: Whether the incidence rate of deep venous thrombosis (DVT) between laparoscopic and open colorectal cancer surgery the same or not were under the debated without conclusion. The aim of this study was to compare the incidence of DVT after laparoscopic or open colorectal cancer surgery by meta-analysis. MATERIALS AND METHODS: The open published articles comparing the incidence of DVT after laparoscopic or open colorectal cancer were collected in the data bases of Medline, the Cochrane central register of controlled trials and CNKI. The relative risk (RR) was pooled by using random or fixed effect mode to evaluate the incidence of DVT between laparoscopic or open colorectal cancer surgery. RESULTS: After searching the databases, 9 randomized clinical studies with 2606 colorectal cancer cases were included in this meta-analysis. The mean operation time was 201.8 ± 17.28 min with its range of 180.0-224.4 min in the laparoscopic surgery group and 148.1 ± 18.8 min with its range of 135.0-184.0 min in the open surgery group. The operation time for laparoscopic surgery group were significant lower than in the open surgery group (P < 0.05). The RR of DVT between the laparoscopy and open surgery groups was 0.71 with its 95% confidence interval of 0.35-1.45 (P = 0.35). CONCLUSIONS: The operation time in laparoscopic colorectal cancer surgery was statistical longer than in the open colorectal cancer surgery, but the DVT risk of the two surgery approach was not different according to this meta-analysis.
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Neoplasias Colorrectales/cirugía , Cirugía Colorrectal/efectos adversos , Laparoscopía/efectos adversos , Complicaciones Posoperatorias , Trombosis de la Vena/etiología , Humanos , Pronóstico , Factores de RiesgoRESUMEN
Rhynchophorus ferrugineus (Coleoptera, Curculionidae) is the most destructive pest of palm trees worldwide containing it invasive areas, such as the southern part of China. It is always emphasized to develop integrated pest management based on biological agents, but their success is not very exciting. Presently, the immune defenses of this pest against biological agents attract scarce attention. It is still unclear whether immune priming also generally occurs in insect pests and in response to different pathogens. Our results indicated that previous challenge of bacteria pathogen enhanced the magnitude of phenoloxidase activity and antibacterial activity in R. ferrugineus larvae against the secondary infection. Furthermore, trans-generational immune priming was also determined in this pest, and only challenged R. ferrugineus mothers transferred the immune protection to their offspring which suggested males and females of this pest might have evolved different strategies on the investment of delivering immune protection to their offspring. Importantly, our data provide the evidence to suggest that different kinds of biological control agents might be used alternatively or in combination to fight against R. ferrugineus because of the existence of immune priming with low species-specific level. On the other hand, for this invasive pest, the immune priming may also facilitate its adaptation and dispersal in the new regions.