Effects of Bacillus amyloliquefaciens and Yarrowia lipolytica lipase 2 on immunology and growth performance of Hybrid sturgeon.

A 12-weeks feeding trial was performed to investigate the possible effects of supplementation of Hybrid sturgeon diet with Bacillus amyloliquefaciens (GB-9) and Yarrowia lipolytica lipase2 (YLL2) single or combined on immune response and growth performance of Hybrid sturgeon (Acipenser schrenkii ♂and Acipenser baeri ♀). For this aim, Hybrid sturgeons were fed with four experimental diets namely: Diet 1 (0-control), Diet 2 (5.0 g/kg GB-9), Diet 3 (4.0 g/kg YLL2), and Diet 4 (5.0 g/kg GB-9 + 4.0 g/kg YLL2), respectively. After fed with varied diets, growth performance, mucosal immune response, leukocytes immune response and serum immunological response were measured. The results indicated that supplementations of GB-9 + YLL2 resulted in a significant increase in final weight, Docosahexaenoic acid (DHA) and Eicosapentenoic acid (EPA) concentration, compared with that of control (p < 0.05). For innate immunity, the results showed that skin mucus lysozyme activity, leukocytes phagocytosis activity and reactive oxygen species level, and serum alternative complement pathway activity, peroxidase and lysozyme activity were significantly higher in supplemented groups compared to the control (p < 0.05). The highest values were recorded in fish fed both YLL2 and GB-9 with respect to the individual application. The present results suggested that the combination of these supplementation could be considered as potential feed-additives for aquaculture farmed fish.

Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression.

The genes encoding HSP70 and HSP90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary DNA (cDNA) ends (RACE). HSP70 (GenBank accession no. KP050541) and HSP90 (GenBank accession no. KP050542) cDNAs were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. Basic Local Alignment Search Tool (BLAST) analysis showed that HSP70 and HSP90 of kaluga shared high identities with those of Acipenser ruthenus, Acipenser schrenckii, and Acipenser baerii (98-99 %). Fluorescent real-time RT-PCR under unstressed conditions revealed that HSP70 and HSP90 were expressed in 11 different tissues of kaluga. Messenger RNA (mRNA) expressions of both HSP70 and HSP90 were highest in the intestine and lowest in the muscle. In addition, the patterns of mRNA expression of HSP70 and HSP90 were similar, although the level of expression was more in HSP90 than in HSP70 (P < 0.05).We also analyzed patterns of HSP70 and HSP90 expression in the muscle, gill, and liver of kaluga under different combinations of temperature and salinity stress, including temperatures of 4,10, 25, and 28 °C at 0 ppt salinity, and salinities of 10, 20, 30, and 40 ppt at 16 °C, where 16 °C at 0 ppt (parts per thousand) served as the control. We found that levels of mRNA expression of both HSP70 and HSP90 were highest at 4 °C in the muscle, gill, and liver and changed little with salinity stress. These results increase understanding of the mechanisms of stress response of cold freshwater fish.

Wavefront aberration compensation of projection lens using clocking lens elements.

For extremely high-performance lithographic lenses, the edge level accuracy of the manufacturing process and multicompensation strategies must be applied. Element clocking can be effectively used to compensate for the low-order figure errors of the elements. Considering that commercial optical software is usually incapable of obtaining good convergence for clocking optimization, this paper proposes a mathematical model of a lithographic lens containing the errors of a surface figure, after which a clocking optimization algorithm is programmed. A clocking optimization instance proving that the clocking optimization algorithm is capable of finding the optimized angle of elements and that clocking is an effective compensation strategy. The calculated accuracy of the proposed mathematic model was found to be acceptable for clocking optimization.

Simulated and experimental study of laser-beam-induced thermal aberrations in precision optical systems.

Precision optical systems that utilize laser beams as working media usually suffer from thermal aberrations caused by absorbed energy. Based on a specially designed three-lens system, the causes and contributions of mechanical structures to the system's thermal aberrations are studied. The contribution of three thermal effects, surface deformation, change of refractive index, and stress birefringence on the system's thermal aberrations, is analyzed respectively through an integrated optomechanical simulation method. The impact of the structure's thermal dissipating capability and structure configuration on the system's thermal aberrations is analyzed, too. Experiments have been carried out to validate the correctness and accuracy of the simulation method. Both the simulated and tested results can provide a reference for structure design and thermal aberration analysis of the similar optical systems.

Mathematical construction and perturbation analysis of Zernike discrete orthogonal points.

Zernike functions are orthogonal within the unit circle, but they are not over the discrete points such as CCD arrays or finite element grids. This will result in reconstruction errors for loss of orthogonality. By using roots of Legendre polynomials, a set of points within the unit circle can be constructed so that Zernike functions over the set are discretely orthogonal. Besides that, the location tolerances of the points are studied by perturbation analysis, and the requirements of the positioning precision are not very strict. Computer simulations show that this approach provides a very accurate wavefront reconstruction with the proposed sampling set.

Design of algorithms for phase shifting interferometry using self-convolution of the rectangle window.

The objective of this paper is to design phase shifting algorithms error-resistant to the nonlinearity of phase-shift error and photoelectric detector simultaneously. An effective construction approach is proposed based on self-convolution of the rectangle window to design algorithms with perfect zero point distribution, according to the fact that the error-resistant capability is entirely determined by the number and order of zero points of Fourier transform of the related window function. Theoretical analysis and numerical simulations compared to the commercial 13-frame algorithm demonstrate the validity of the approach to design algorithms with enhanced error-resistant capability not only to CCD-caused harmonics but also to PZT ramping nonlinearity.