[Protective effect of N-stearoylethanolamine in acute alcohol intoxication in rats].

The influence of N-stearoylethanolamine on the nitric oxide system, the state of lipid peroxidation, antioxidant enzyme activity, content of phospholipids and fatty acids were studied under the acute ethanol intoxication (2.5 g/kg) in rats. The results of investigations show that acute ethanol intoxication caused abnormalities of the oxidative homeostasis accompanied by the accumulation of thiobarbituric acid reactive substances (TBARS). High catalase and glutathione peroxidase activity was also shown. The altered content of total and individual fatty acids of phospholipids in the rat liver tissue was found. The content of saturated fatty acids (palmitic, stearic) increased and amount of unsaturated (palmitoleic, oleic, linolenic) acids decreased under acute ethanol intoxication. The changes of nitric oxide content was found in the brain, plasma and red blood cells. N-stearoylethanolamine (NSE) in a dose of 50 mg/kg of body weight shows the pronounced antioxidative and hepatoprotective properties under these conditions. It was found that the preliminary NSE administration to rats inhibited accumulation of TBARS and caused a simultaneous increase of antioxidant enzyme activity. The NSE administration modulated also the content of total and individual fatty acids of phospholipids and the amount of nitric oxide in pathologically altered tissues. These results suggested that NSE protected the structural integrity and functional ability of cell membranes under the acute ethanol intoxication.

[The modelling of N-stearoylethanolamine effect of 17beta-estradiol in the organism of male rats].

The influence of N-stearoylethanolamine (NSE) on total 11-hydroxycorticosteroids (11-HCS) and testosterone level in the blood of male rats in normal conditions and under the action of 17beta-estradiol (400 mkg/kg of body weight during 3 days) was studied. It was shown that NSE administration per os (50 mg/kg of body weight during 7 days) to intact animals did not change the level of 11-HCS and of testosterone. The administration of NSE to estrogenized male rats decreased the elevated level of 11-HCS and normalized the amount of testosterone in blood. The correction of alterated weight of adenohypophysis and testis of estrogenized male rats compared to control can be a direct evidence of NSE-mediated modelling of the effect on hypothalamic-pituitary hormone system. The effect of NSE in the testis of estrogenized male rats inhibited the process of lipid peroxidation, caused the decrease of the amount of thiobarbituric acid reactive substances and increased the activity of superoxide dismutase and catalase. The NSE showed more expressed antioxidative effect compared to vitamin E. Taking into consideration all above mentioned data we suggested that NSE administration to male rats protected Leydig cells from damage under the increase of estrogen level.

[Effect of N-stearoylethanolamine on the lipid peroxidation process and lipid composition of the rat liver in acute morphine intoxication].

The effect of N-stearoylethanolamine (NSE) on the lipid peroxidation process, antioxidant enzymes activity, phospholipid and fatty acid content in the rat liver tissues under acute morphine administration was studied. It was shown that morphine administration (30 mg/kg of body weight) caused an increase of the amount of thiobarbituric acid reactive substances (TBARS), alteration of antioxidant enzymes activity, decrease the protein level, quantity of total lipids and phospholipids, phosphatidylcholine, cholesterol esters; altered the content of some individual fatty acids. NSE administration (50 mg/kg of body weight) promoted normalization of the antioxidant enzymes activity and prevented the TBARS accumulation and decreased the total lipid and phospholipid quantity, increased the content of free and total cholesterol, corrected the level of free and individual fatty acids. It was assumed that NSE possessed antioxidative, membranoprotective and adaptive properties.

[Distribution of fatty acids of plasmalogenic phospholipids in human erythrocytes and endometrium in hyperplasia of various type]. / Rozpodil zhyrnykh kyslot plazmalohennykh fosfolipidiv v erytrotsytakh krovi ta v endometriï liudyny pry hiperplaziï riznoho kharakteru.

It is shown that development of hyperplasia of endometrium (PPPE) is accompanied by the valid decrease of plasmenyl-phosphatidylethanolamine (PL PhEA) amount both in the blood erythrocytes and human endometrium and by the increase of diacylglycerophosphoethanolamin (DG PhEA). Data obtained evidence for the development of the state of plasmalogenic phospholipids insufficiency in the organism. When investigating the composition and quantity of fatty acids of plasmalogenic phospholipids of PhEA in the blood erythrocytes of patients it was established that the amount of arachidonic (C 20:4 omega-6) and docosahexaenoic (C 22:6 omega-3) reliably decreases more than by 50%. At the same time one can observe the reliable increase of palmitic (C 16:0) and stearic (C 18:0) fatty acids. Under these conditions the ratios omega-6/omega-3 of both arahidonic and docosahexaenoic (AA/DHA) PL PhEA in the composition of erythrocyte PhEA increase with hyperplasia development 4 and 2 times respectively, but in DPhG the above ratios decreased 1.5 times. The decrease of PUFA in erythrocytes evidences for the increase of their use for restoration of their level disturbances in the organism. As a result of analysis of fatty acids content in tissues at hyperplasia development, considerable decrease of AA and DHA was established in one group of examined patients compared with the control, as well as the increase of omega-6/omega-3 2.8 times, and AA/DHA 2 times. Reliable double increase of AA and eicosatrienoic acid and decrease of DHA and linolenic acid was observed in group 2 of the patient with adenomatosic hyperplasia. The ratio omega-6/omega-3 and AA/DHA decreased twice in the both groups as compared with the control, that evidenced for development of hyperplasia of different origin. Specific distribution of arachidonic acid in plasmalogenic phospholipids at adenomatosic hyperplasia is connected with decrease of plasmalogens content and increase of formation of prostaglandin E2.

[Specificity of endometrium phospholipids and their fatty acids changes in the development of hyperplasia]. / Spetsyfichnist' zmin vmistu fosfolipidiv i ïkh zhyrnykh kyslot v endometriï pry rozvytku hiperplaziï.

Quantitative changes in phospholipids (PL) and fatty acids content (FA) in patients with endometrium hyperplasia have been revealed. During observing endomentrium hyperplasia process we found out on the one hand reliable decrease in phosphatidylethanolamine (PEA), phosphatidylcholine (PC) content and on the other hand increase of its lysoforms. Changes in unsaturated fatty acids (PA n-6)--arachidonic (AA) content and docosahaenoic (DHA) acids content were also shown. The decrease of PC, PEA in patients with endometrium hyperplasia is interrelated with quantitative change of plasmalogenic phospholipids PL, which is too much sensitive to oxidative reactions and at the same time able to protect sells against oxidative stress. Considerable decrease in plasmenyl PEA content on the one side and plasmenyl PC content stability on the other was revealed in patients with endometrium hyperplasia. Above mentioned lipid metabolic derangements of the endometrium are specific with respect to different classes of phospholipids and their fatty acids composition.

[Effect of phospholipids containing omega-3 fatty acids on structural changes of microsomal lipids in cell membranes of functionally different cells]. / Vplyv fosfolipidiv, shcho mistiat' omeha-3 zhyrni kysloty, na strukturni zminy lipidiv mikrosom membran klityn, riznykh za funktsiieiu.

As a result of the experimental researches conducted it has been shown that administration of some normal animal marine phospholipids (PL) including in their structure omega-3 polyunsaturated fatty acids (PUFA) provides for quantitative changes of individual PL, fatty acids (FA) content and quantity in general and individual PL of liver, heart, brain and gonads microsomes. While estimating general microsomal PL fraction FA content under the action of PL omega-3 PUFA FA concentration change, unsaturation index (omega 6/omega 3) and relation of arachidonic acid to docosahexenic (AA/DHA) decrease have been identified. The decrease of AA/DHA relationship occurs due to AA and DHA quantitative changes. In the case of AA increase in some tissues there is observed the decrease of docosapentaenic acid and increase of DHA and eucosapentaenic (EPA) acidds. As a result of studying FA content in the individual PL composition it has been identified that certain PL classes characteristic for some tissues respond by changes of some certain FA. The relationship omega 6/omega 3 has been shown as decreasing in phosphatidilcholine (PC) all tissues microsomes (liver, gonads, heart, brain), in phosphatidilethanolamine (PEA) of liver and cardiac microsomes, in phosphatidilserine (PS) this relationship relationship decreases in the liver, brain and heart, for phosphatidilinositole (PI) the changes take place in liver, gonads, brain. Simultaneously, the decrease of AA/DHA relationship in the individual PL decrease of AA and increase of EPA and DHA depend on the tested tissues. The marine phospholipids might be supposed to render their effect on AA metabolism resulting in AA/DHA relationship in PEA and PS relationship displays itself as specific and depends on the tissues functions. The preference of PEA and PS use by certain tissues microsomes could be explained by their membrane protective capability.