RESUMEN
The study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique (LISS-Coombs indirect antiglobulin test), and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity.
Asunto(s)
Anticuerpos/sangre , Prueba de Coombs/métodos , Pruebas de Hemaglutinación/métodos , Tamizaje Masivo/métodos , Estudios de Casos y Controles , Prueba de Coombs/economía , Prueba de Coombs/instrumentación , Prueba de Coombs/normas , Análisis Costo-Beneficio , Pruebas de Hemaglutinación/economía , Pruebas de Hemaglutinación/instrumentación , Pruebas de Hemaglutinación/normas , Humanos , Isoanticuerpos/sangre , Jordania , Tamizaje Masivo/normas , Papaína , Globulina Inmune rho(D)/sangre , Sensibilidad y EspecificidadRESUMEN
The study aimed to determine the specificity and sensitivity of the Ortho BioVue two-column agglutination system for the detection of low concentrations of clinically significant antibodies in serum. The BioVue system was compared with the conventional tube technique [LISS-Coombs indirect antiglobulin test], and the two-stage Papenzyme test was used to resolve discrepancies between the two methods. We tested 3000 serum samples from randomly selected patients at King Hussein Medical Centre. Both the antibody screening and identification gave negative results in 2952 patients and positive results in 48 patients. We found the BioVue system to be the more sensitive technique. However, if papain enzyme-treated cells were included in the conventional tube technique when applied to antibody screening and identification, both methods would be of comparable sensitivity
