Evaluation of Polycyclic Aromatic Hydrocarbons in Smoked Cheeses Made in Poland by HPLC Method.

Smoked cheeses are particularly popular among consumers for their flavor and aroma. Of interest, therefore, is the health aspect related to the likelihood of polycyclic aromatic hydrocarbons (PAHs), which are known carcinogens found in smoked products. Thus, the purpose of this study was to evaluate the occurrence of 15 polycyclic aromatic hydrocarbons (PAHs) in smoked and non-smoked cheeses purchased in Poland to monitor their safety. The level of selected PAHs in cheese samples was determined using the HPLC-DAD-FLD method. Most of the cheeses tested met the maximum level of benzo[a]pyrene (2 µg/kg) and the sum of benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene (12 µg/kg) established for these products. However, all the cheeses studied in this work had relatively low amounts of the sum of these compounds compared to the information available in the cheese literature, ranging from

Alginate Biofunctional Films Modified with Melanin from Watermelon Seeds and Zinc Oxide/Silver Nanoparticles.

Bioactive films find more and more applications in various industries, including packaging and biomedicine. This work describes the preparation, characterization and physicochemical, antioxidant and antimicrobial properties of alginate films modified with melanin from watermelon (Citrullus lanatus) seeds at concentrations of 0.10%, 0.25% and 0.50% w/w and with silver and zinc oxide nanoparticles (10 mM film casting solutions for both metal nanoparticles). Melanin served as the active ingredient of the film and as a nanoparticle stabilizer. The additives affected the color, antioxidant (~90% ABTS and DPPH radicals scavenging for all melanin modified films) and antimicrobial activity (up to 4 mm grow inhibition zones of E. coli and S. aureus for both zinc oxide and silver nanoparticles), mechanical (silver nanoparticles addition effected two-fold higher tensile strength), thermal and barrier properties for water and UV-vis radiation. The addition of ZnONP resulted in improved UV barrier properties while maintaining good visible light transmittance, whereas AgNP resulted in almost complete UV barrier and reduced visible light transmittance of the obtained films. What is more, the obtained films did not have an adverse effect on cell viability in cytotoxicity screening. These films may have potential applications in food packaging or biomedical applications.

Preparation and Characterization of Carboxymethyl Cellulose-Based Bioactive Composite Films Modified with Fungal Melanin and Carvacrol.

Preparation of biodegradable packaging materials and valorisation of food industry residues to achieve "zero waste" goals is still a major challenge. Herein, biopolymer-based (carboxymethyl cellulose-CMC) bioactive films were prepared by the addition, alone or in combination, of carvacrol and fungal melanin isolated from champignon mushroom (Agaricus bisporus) agro-industrial residues. The mechanical, optical, thermal, water vapour, and UV-Vis barrier properties were studied. Fourier-transform infrared (FT-IR) spectroscopy studies were carried out to analyse the chemical composition of the resulting films. Antibacterial, antifungal, and antioxidant activities were also determined. Both CMC/melanin and CMC/melanin/carvacrol films showed some antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. The addition of melanin increased the UV-blocking, mechanical, water vapour barrier, and antioxidant properties without substantially reducing the transparency of the films. The addition of carvacrol caused loss of transparency, however, composite CMC/melanin/carvacrol films showed excellent antioxidant activity and enhanced mechanical strength. The developed bioactive biopolymer films have a good potential to be green bioactive alternatives to plastic films in food packaging applications.

Whey Protein Concentrate/Isolate Biofunctional Films Modified with Melanin from Watermelon (Citrullus lanatus) Seeds.

Valorization of food industry waste and plant residues represents an attractive path towards obtaining biodegradable materials and achieving "zero waste" goals. Here, melanin was isolated from watermelon (Citrullus lanatus) seeds and used as a modifier for whey protein concentrate and isolate films (WPC and WPI) at two concentrations (0.1% and 0.5%). The modification with melanin enhanced the ultraviolet (UV) blocking, water vapor barrier, swelling, and mechanical properties of the WPC/WPI films, in addition to affecting the apparent color. The modified WPC/WPI films also exhibited high antioxidant activity, but no cytotoxicity. Overall, the effects were melanin concentration-dependent. Thus, melanin from watermelon seeds can be used as a functional modifier to develop bioactive biopolymer films with good potential to be exploited in food packaging and biomedical applications.