RESUMEN
Pheromonal volatiles emitted by irradiated and control 5- to 11-day-old Caribbean fruit flies,Anastrepha suspensa (Loew), were collected on Tenax adsorbent filters and quantified by gas chromatography (GC). The components released were identified by comparison of retention times on GC and by mass spectrometry (MS) with authentic synthetic standards. Pharate adults were irradiated with gamma rays from a(60)Co source at a dose rate of 10.3 Gray (Gy)/min. The total dosages given were 30, 50, 70, and 100 Gy. Pheromone volatiles were collected from adult males when they were between 5 and 11 days of age. The compounds quantified were (Z)-3-nonenol and (Z, Z)-3,6-nonadienol, which eluted from the GC column together and were quantified as one peak,ß-bisabolene, suspensolide, anastrephin, and epianastrephin. Irradiation with 30 Gy did not significantly reduce any pheromonal components, nor did it change the pheromonal blend. In contrast, suspensolide and bisabolene were significantly reduced in flies irradiated with 50 Gy, while the nonenols and epianastrephin were reduced at the 70-Gy dose. Irradiation with the 100-Gy dose reduced all components with the exception of suspensolide.
RESUMEN
Females and eggs of Toxorhynchites r. rutilus were labeled with 32P by feeding fourth-stage larvae 32P labeled Aedes aegypti larvae. Eggs from females up to 3 weeks in age had detectable levels of radioactivity and individual eggs contained ca. 0.3% of the mother's total radioactivity. Comparisons of labeled and unlabeled females in indoor and outdoor cage tests indicated that survival and fecundity of the 2 groups were approximately equal. No differences were noted for dispersal and fecundity of labeled and control females released in field tests. The 32P-labeled Tx. r. rutilus females behave similarly to unlabeled females, and this method of radiolabeling provides a sound tool for tracking laboratory-reared females released into an area with an indigenous population.
