RESUMEN
Lactoferrin (LF), the glycoprotein transferring Fe+ ions, is contained in barrier liquids, human blood and milk. LF is an acute phase protein and one of the most important factors of nonspecific defense. The protein has unique set of biological functions. Using the methods of small-angle X-ray scattering and light-scattering it was shown for the first time that addition of DNA and oligosaccharides to LF with different level of initial oligomerization leads to an enhance in the oligomerization rate. 1M NaCl stimulates almost a complete dissociation of LF oligomeric complexes obtained in the pesence of DNA, oligosaccharides, or early founded oligomerization effectors (nucleotides). It was shown that LF oligomeric complexes obtained in the presence of different oligomerization effectors have different stability. Incubation with 50 mM MgCl2 leads to complete destruction of the protein complexes formed by ATP and oligosaccharide but partially dissociate the complexes with following formation of new in the case of AMP- and d(pT)10-dependent associates, which possess higher stability i n presence of the salt. A possible role of LF oligomerization for different biological functions of the protein is discussed.
Asunto(s)
Proteínas Portadoras/química , ADN/química , Hierro/química , Oligosacáridos/química , Adenosina Monofosfato/química , Adenosina Monofosfato/metabolismo , Adenosina Trifosfato/química , Adenosina Trifosfato/metabolismo , Proteínas Portadoras/metabolismo , ADN/metabolismo , Humanos , Hierro/metabolismo , Lactoferrina , Oligosacáridos/metabolismo , Estabilidad Proteica , Estructura Cuaternaria de Proteína/fisiologíaRESUMEN
A complex multi-staged method for stimulation of bone tissue regeneration in the jaws after removal of teeth and preparation of bone tissue for subsequent implantation, stimulation of bone tissue regeneration after implantation, and adaptation of bone tissue of the jaws to masticatory pressure is developed. The method is based on utilization of magnetic laser exposure with a universal Optodan laser device. In case of pronounced inflammation and pyodestructive processes after implantation, these processes are liquidated due to detoxifying effect of the blood, which is stimulated by irradiation of the carotid sinuses on both sides. Granulation tissue round the heads of implants is removed by laser coagulation with surgical lasers Lancet or Stokos.
