RESUMEN
BACKGROUND: It has been reported recently that long-term alcohol exposure in rats increases the number of dihydropyridine binding sites in cardiac membrane preparations. We fed Sprague Dawley rats a liquid diet that contained ethanol as 36% of total calories for 4 to 6 months and studied how alcohol exposure affected the activity and regulation of the cardiac Ca2+ channel. METHODS: Dihydropyridine-sensitive cardiac Ca2+ channel activity was measured as the rate of Mn2+ quench of the cytosolic fura-2 signal in electrically stimulated myocytes. RESULTS: In control rat myocytes, pretreatment with phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC), reduced the rate of Mn2+ quench to 68% of the untreated cell response. Pretreatment with GF109203X, a protein kinase C inhibitor, enhanced the rate of influx by 56%, whereas Gö6976, an inhibitor of PKC alpha, beta, and gamma, did not affect the rate of influx. By contrast, PMA did not affect the rate of Mn2+ quench in alcoholic myocytes; however, the PKC inhibitor GF109203X still enhanced the rate of Mn2+ quench by 33%. Similar to control myocytes, no effect was observed after pretreatment with Gö6976 in the alcoholic cells. In both Western blot and immunoprecipitation experiments, PKC epsilon expression in alcohol-exposed myocytes was reduced to 68% of the control. However, the ratio of membrane/ cytosolic distribution of PKC epsilon in alcoholic myocytes was increased from 1.6 to 2.6. No change was detected in the expression of PKC alpha and PKC delta. PKC activity, measured in the presence of Gö6976, which inhibits PKC alpha, beta, and gamma, was reduced in alcoholic myocytes to 57% of the control, but the proportion of PKC activity in the particulate fraction was increased from 26% in the control myocytes to 36% in the alcoholic myocytes. CONCLUSIONS: Altered expression and activity of PKC may be associated with changes in the regulation of the cardiac Ca2+ channel found in the hearts of rats chronically exposed to alcohol. Specifically, we found that the novel class of PKC isozymes is responsible for regulating the cardiac Ca2+ channel in control cardiomyocytes, and that the loss of PMA modulation found in the alcoholic cells may be due, in part, to reduced expression and altered distribution of PKC epsilon.
Asunto(s)
Alcoholismo/enzimología , Canales de Calcio Tipo L/fisiología , Etanol/administración & dosificación , Miocardio/enzimología , Proteína Quinasa C/fisiología , Animales , Estimulación Eléctrica , Inhibidores Enzimáticos/farmacología , Indoles/farmacología , Isoenzimas/análisis , Isoenzimas/antagonistas & inhibidores , Isoenzimas/fisiología , Maleimidas/farmacología , Manganeso/metabolismo , Proteína Quinasa C/análisis , Proteína Quinasa C/antagonistas & inhibidores , Ratas , Ratas Sprague-Dawley , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Acute exposure to interleukin 1 beta (IL1beta) or insulin-like growth factor 1 (IGF1) promoted the translocation of PKC alpha from the cytosol to the membrane of adult rat cardiomyocytes. Western analysis demonstrated that membranal localization of PKC alpha was increased from 23% in the control to 49% after exposure to IGF1, and it was increased to 42% after exposure to IL1beta. Activation of Erk1/Erk2 by IGF1 and IL1beta was studied using a phosphorylation-specific antibody. IGF1-induced activation of p44/p42 MAP kinase was blocked by preincubation with the PKC inhibitors, bisindolylmaleimide and Gö6976, as well as the tyrosine kinase inhibitor, genistein. IGF1 increased the rate of protein synthesis, indicated by the increase in L-[(14)C(U)] phenylalanine incorporation over time, and this effect was inhibited by Gö6976.
Asunto(s)
Factor I del Crecimiento Similar a la Insulina/farmacología , Interleucina-1/farmacología , Isoenzimas/metabolismo , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Miocardio/citología , Biosíntesis de Proteínas , Proteína Quinasa C/metabolismo , Animales , Canales de Calcio Tipo T/efectos de los fármacos , Canales de Calcio Tipo T/fisiología , Carbazoles/farmacología , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Genisteína/farmacología , Immunoblotting , Indoles/farmacología , Isoenzimas/antagonistas & inhibidores , Sistema de Señalización de MAP Quinasas/fisiología , Masculino , Maleimidas/farmacología , Manganeso/metabolismo , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Miocardio/metabolismo , Biosíntesis de Proteínas/efectos de los fármacos , Proteína Quinasa C/antagonistas & inhibidores , Proteína Quinasa C-alfa , Transporte de Proteínas/efectos de los fármacos , RatasRESUMEN
Although there are several reports on the regulation of neuronal and skeletal muscle voltage-sensitive calcium channels by IGF1, the effects of short-term IGF1 exposure on cardiac Ca2+ channels have not been described. We measured the activity of nitrendipine-sensitive Ca2+ channels of intact cardiac myocytes in the presence of IGF1 by monitoring unidirectional Mn2+ influx measured as the quench of cytosolic fura-2 in electrically stimulated or K+-depolarized cells. Maximal channel activation was observed after 10 min of preincubation with IGF1, which gave an increase of 216 +/- 25%. Treatment with the protein kinase C inhibitors bisindolylmaleimide I and chelerythrine mimicked the augmentation effect of IGF1, whereas PMA blocked enhancement of Mn2+ influx by IGF1. These results demonstrate that acute IGF1 augments dihydropyridine-sensitive sarcolemmal Ca2+ channel activity and that protein kinase C may contribute to the regulation of cardiac Ca2+ channels by IGF1.
Asunto(s)
Canales de Calcio/fisiología , Corazón/fisiología , Factor I del Crecimiento Similar a la Insulina/farmacología , Sulfonamidas , Animales , Calcio/metabolismo , Canales de Calcio/efectos de los fármacos , Carbazoles/farmacología , Células Cultivadas , Cromonas/farmacología , Citosol/metabolismo , Inhibidores Enzimáticos/farmacología , Humanos , Indoles/farmacología , Factor I del Crecimiento Similar a la Insulina/fisiología , Isoquinolinas/farmacología , Cinética , Masculino , Manganeso/metabolismo , Morfolinas/farmacología , Proteína Quinasa C/antagonistas & inhibidores , Proteína Quinasa C/metabolismo , Ratas , Ratas Sprague-Dawley , Proteínas Recombinantes/farmacología , Sarcolema/metabolismo , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Ethanol inhibits L-type Ca++ channels, but little is known about its effect on other voltage-gated Ca++ channels. To examine non-L-type channels we used nerve growth factor-differentiated PC12 cells treated with the L channel blocker nifedipine. Using selective Ca++ channel antagonists, we found that N-type and P/Q-type channels mediate most of the remaining depolarization-evoked Ca++ rise. Ethanol (10-150 mM) inhibited depolarization-induced rises in intracellular Ca++ with maximal inhibition of 46% achieved using 50 mM ethanol. Inhibition was time dependent, requiring at least 8 min to develop fully. Ethanol did not alter Ca++ mobilization, sequestration, extrusion or capacitative entry. Sp-adenosine cyclic 3',5'-phosphorothioate, a specific activator of protein kinase A (PKA), blocked inhibition by ethanol, whereas the protein kinase C activator phorbol 12-myristate, 13-acetate did not. Okadaic acid, an inhibitor of protein phosphatases type-1 and type-2A, also blocked inhibition by ethanol with an IC50 of 3 nM. This was prevented by inhibiting PKA, indicating that the action of okadaic acid was due to increased PKA-mediated phosphorylation. These results indicate that ethanol can inhibit N-type and P/Q-type channels and this is antagonized by activating PKA. The findings suggest the sensitivity of these channels to ethanol is regulated by a phosphoprotein that is a substrate for PKA and protein phosphatase type-2A.
Asunto(s)
Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio/efectos de los fármacos , Proteínas Quinasas Dependientes de AMP Cíclico/fisiología , Etanol/farmacología , Animales , Bradiquinina/farmacología , Calcio/metabolismo , Células PC12 , Potasio/farmacología , Ratas , Sodio/metabolismoRESUMEN
Neuronal activity is required for normal neural development. Excessive activity can cause abnormal growth of neural processes and may contribute to formation of epileptic foci. Using PC12 cells, we investigated mechanisms by which depolarization regulates neurite growth. Depolarization with 45 mM KCl induced neurite outgrowth only if NGF receptors were partly activated by overexpression of p140trkA or by treatment with a low concentration of NGF that alone was insufficient to stimulate neurite formation. Depolarization-induced neurite growth was reduced by inhibitors of L-type Ca2+ channels, Ca2+/calmodulin-dependent protein (CaM) kinases II and IV, and transcription. These results identify a novel mechanism by which depolarizing stimuli synergize with subthreshold activation of NGF receptors to induce neurite growth through a Ca2+ and CaM kinase-dependent signal transduction pathway.
Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Neuritas/fisiología , Receptores de Factor de Crecimiento Nervioso/fisiología , Animales , Calcio/metabolismo , Canales de Calcio/metabolismo , Diferenciación Celular , Electrofisiología , Activación Enzimática , Neuritas/efectos de los fármacos , Células PC12 , Proteínas Tirosina Quinasas/metabolismo , Ratas , Transcripción GenéticaRESUMEN
The serum-free mouse embryo (SFME) cell line was isolated from 16-day-old Balb/c mouse embryos in medium in which the usual serum supplement to the culture medium was replaced by purified growth factors and other components. SFME is an unusual line that does not undergo senescence in vitro, maintains an apparently normal karyotype, and is growth inhibited by serum. Transforming growth factor beta (TGF beta) or calf serum induces expression of the astrocyte marker glial fibrillary acidic protein (GFAP) in these cells, and similar cells can be isolated directly from brain. By differential screening of a cDNA library derived from SFME cells, a calf serum- and TGF beta-regulated 8.5 kb mRNA was identified in SFME cells and the cDNA partially sequenced. This mRNA was detected only in RNA preparations from brain among a number of tissues examined, and may provide an additional marker of TGF beta-regulated differentiation in these cells.
Asunto(s)
Química Encefálica/fisiología , Linfotoxina-alfa/fisiología , ARN Mensajero/metabolismo , Animales , Secuencia de Bases , Northern Blotting , Células Cultivadas , Proteínas del Líquido Cefalorraquídeo/biosíntesis , Medio de Cultivo Libre de Suero , Cicloheximida/farmacología , Cistatina C , Cistatinas/biosíntesis , ADN/metabolismo , Sondas de ADN , Embrión de Mamíferos/metabolismo , Femenino , Proteína Ácida Fibrilar de la Glía/biosíntesis , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , EmbarazoRESUMEN
Human S-protein is a serum glycoprotein that binds and inhibits the activated complement complex, mediates coagulation through interaction with antithrombin III and plasminogen activator inhibitor I, and also functions as a cell adhesion protein through interactions with extracellular matrix and cell plasma membranes. A full length cDNA clone for human S-protein was isolated from a lambda gt11 cDNA library of mRNA from the HepG2 hepatocellular carcinoma cell line using mixed oligonucleotide sequences predicted from the amino-terminal amino acid sequence of human S-protein. The cDNA clone in lambda was subcloned into pUC18 for Southern and Northern blot experiments. Hybridization with radiolabeled human S-protein cDNA revealed a single copy gene encoding S-protein in human and mouse genomic DNA. In addition, the S-protein gene was detected in monkey, rat, dog, cow and rabbit genomic DNA. A 1.7 Kb mRNA for S-protein was detected in RNA from human liver and from the PLC/PRF5 human hepatoma cell line. No S-protein mRNA was detected in mRNA from human lung, placenta, or leukocytes or in total RNA from cultured human embryonal rhabdomyosarcoma (RD cell line) or cultured human fibroblasts from embryonic lung (IMR90 cell line) and neonatal foreskin. A 1.6 Kb mRNA for S-protein was detected in mRNA from mouse liver and brain. No S-protein mRNA was detected in mRNA from mouse skeletal muscle, kidney, heart or testis.
Asunto(s)
Glicoproteínas de Membrana/genética , ARN Mensajero/genética , Secuencia de Aminoácidos , Animales , Northern Blotting , Southern Blotting , Línea Celular , Clonación Molecular , Biblioteca de Genes , Humanos , Masculino , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , ARN Mensajero/análisis , Células Tumorales Cultivadas , VitronectinaRESUMEN
Differential screening of a cDNA library derived from mRNA of TGF beta-treated serum-free mouse embryo (astrocyte precursor) cells isolated a strongly TGF beta-regulated mRNA that codes for cystatin C, a cysteine protease inhibitor. Increase in cystatin C mRNA level was observed within four hours after treatment with picomolar concentrations of TGF beta. The increase was reversible upon removal of TGF beta and was not prevented by cycloheximide. These results suggest that cystatin C expression may represent a developmentally regulated differentiated function of astrocytes, and also suggest that cystatin C expression may be involved in the response of brain cells to platelet release of TGF beta after trauma or injury.
Asunto(s)
Cistatinas/genética , Factor de Crecimiento Transformador beta/farmacología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Células Cultivadas , Proteínas del Líquido Cefalorraquídeo/genética , Cicloheximida/farmacología , Cistatina C , Cistatinas/biosíntesis , Embrión de Mamíferos , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , ARN Mensajero/genética , Transcripción Genética/efectos de los fármacosRESUMEN
Three new dihydroxyicosanoids, 12(R),13(R)-dihydroxyicosa-5(Z),8(Z),10(E),14(Z)-tetraenoic acid, 12(R),13(R)-dihydroxyicosa-5(Z),8(Z),10(E),14(Z),17(Z)-pentaeno ic acid and 10(R*),11(R*)-dihydroxyoctadeca-6(Z),8(E),12(Z)-trienoic acid, have been isolated from a previously unstudied temperate red marine alga, Farlowia mollis (Cryptonemiales, Rhodophyta). The structures of these new metabolites have been deduced from detailed nuclear magnetic resonance and mass spectrometry analyses on stabilized diacetate-methyl esters and stereochemistry deduced by 1H NMR couplings and CD analysis of a dibenzoate derivative. Collectively, these new natural products modulate fMLP-induced superoxide anion generation in human neutrophils, inhibit the conversion of arachidonic acid to lipoxygenase products by human neutrophils, and inhibit the functioning of the dog kidney Na+/K+ ATPase.