Development of a plating medium for selection of Helicobacter pylori from water samples.

The goal of this study was to develop a simple plating medium to allow large-scale screening of water samples for the presence of Helicobacter pylori. Five conventional plating media (brain heart infusion, brucella agar, Columbia blood agar base, campylobacter agar kit Skirrow, and HPSPA medium), each containing a commercial antibiotic supplement, were initially evaluated. Eight strains selected as common waterborne organisms (Acinetobacter, Aeromonas, Bacillus, Escherichia coli, Enterobacter, Enterococcus, Helicobacter pylori, and Pseudomonas strains) were individually plated onto each of these media. Three organisms (Acinetobacter, E. coli, and H. pylori) were able to grow on all five media. This growth was unacceptable since Helicobacter grows very slowly and competing organisms must be inhibited for up to 7 days. Therefore, a more selective medium (HP agar) containing a novel mixture of growth supplements plus amphotericin B and polymyxin B was developed. This medium also included a phenol red color indicator for urease production. Aliquots of nonsterile well water that contained native flora (Flavobacterium, Serratia, Citrobacter, Pasteurella, Ochrobactrum, Rahnella, and unidentified molds) and were further adulterated with the eight strains listed above (10(6) CFU of each strain per 100 ml) were spiked with H. pylori and were plated. In spite of the heavy mixed microbial load, only H. pylori colonies grew during 7 days of incubation at 37 degrees C. The color indicator system allowed presumptive identification of H. pylori colonies sooner (12 to 20 h) than the conventional media tested allowed. The HP formulation developed in this study provides a medium with superior selectivity for H. pylori from mixed microbial populations in water and reduces the time required to complete the assay.

Comparison of polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) in Lake Michigan salmonids.

Polybrominated diphenyl ethers (PBDEs) have been used extensively over the past two decades as flame retardants in most types of polymers. Many measurements of PBDEs in various environmental matrices from Sweden, Holland, Japan, and elsewhere have been reported, but few measurements are available for North America. PBDEs in 21 coho and chinook salmon taken from Lake Michigan tributaries in 1996 were measured for this study. The salmon samples were extracted and initially analyzed for polychlorinated biphenyl (PCB) congeners. It was demonstrated for these samples that the same extract fraction contains PBDEs. Six PBDE congeners were observed in all 21 samples, and the rank order of concentration of these congeners was similar to that in commercial mixtures of PBDEs. The average concentration across all samples of the sum of PBDE congeners was 80.1 ng/g of wet weight or 2,440 ng/g of lipid. This is much less than the average sum PCB concentration (1,450 ng/g of wet weight; 43,100 ng/g of lipid). However, the average concentration of the most abundant PBDE congener (IUPAC BDE-49: 52.1 ng/g wet, 1,590 ng/g of lipid) was about one-third of the average concentration of the most abundant PCB congener (IUPAC CB-153: 149 ng/g wet, 4,550 ng/g of lipid). On the basis of an extensive literature survey, the concentrations of PBDEs reported here are among the highest in the world for salmon in open waters. The concentrations of PBDEs and PCBs are both correlated with fish length and mass, but not with lipid content. The concentrations of PBDEs and PCBs are highly correlated in individual fish, implying that PBDEs are as prevalent as PCBs in Lake Michigan.

Urinalysis of atrazine exposure in farm pesticide applicators.

This study compared three relatively common laboratory methods for the detection of atrazine (a triazine herbicide commonly used in US agriculture), and related metabolites in urine. Ninety-nine samples collected from atrazine applicators within 8 h post application were analyzed. Thirty-seven percent of applicators showed detectable levels (minimum = 1.0 ng/ml) of deethylatrazine (an atrazine metabolite typically found in environmental samples) in their urine, based on gas chromatography-mass spectrometry (GC-MS) analysis (mean = 14.2 ng/ml). Fifty applicator samples were tested using an enzyme-linked immunosorbent assay (ELISA) designed for the mercapturate metabolic product. Most of these samples (80%) had detectable levels of the mercapturate product. A triazine in water ELISA was also used to test several diluted urine samples from atrazine applicators, and all samples were positive for triazines. Mediocre agreements between the three methods indicated that each detected distinct atrazine exposure products. The results indicate that single field applications of atrazine result in measurable pesticide doses to applicators and that the choice of field assay should depend on the exposure product to be evaluated.

Correlations among human blood levels of specific PCB congeners and implications for epidemiologic studies.

Specific congeners of PCBs may differ with respect to their human health risks. For epidemiologic studies, however, measuring levels of specific congeners--as compared with estimating the concentration of total PCBs present, may be of limited value if levels of specific congeners are highly correlated. We examined the correlations among levels of specific congeners in three groups: controls from a case-control study of breast cancer in North Carolina and two groups from Wisconsin with exposure to fish from contaminated waters. Levels of specific congeners were, in general, highly correlated (Pearson r > 0.80). However, the level of congener 180, a heptachlorobiphenyl, tended to be less correlated with levels of lower-chlorinated biphenyls. Among the implications of these findings are that measurement of a select group of congeners may yield essentially the same information as measurement of a large panel, and may be more cost efficient.

Weighted least-squares approach to calculating limits of detection and quantification by modeling variability as a function of concentration.

The limit of detection and limit of quantification are current critical issues in environmental testing. In most laboratories, limits are currently calculated on the basis of the standard deviation of replicate analyses at a single concentration. However, since the standard deviation depends on concentration, these single-concentration techniques result in limits that are directly dependent on spiking concentration. A more rigorous approach uses a weighted least-squares regression analysis of replicates spiked at a series of concentrations [Formula: see text] a calibration design. In this work, the use of weighted tolerance intervals is introduced for estimating detection and quantification limits. In addition, models for estimating the weights used in calculating weighted prediction intervals and weighted tolerance intervals are presented. Using this method, detection and quantification limits were calculated for gas chromatographic analyses of 16 polychlorinated biphenyls. Results show that the approach developed provides improved estimates of analytical limits and that the single-concentration approaches currently in wide use are seriously flawed. Future work should reduce the data needed for the calibration design approach so that more rigorous detection and quantification limits can be routinely applied.

Fish consumption and reproductive outcomes in Green Bay, Wisconsin.

Prenatal exposure to polychlorinated biphenyls (PCBs) and reproductive outcome was determined in a population of 1112 women during 1987-1989. The women studied were from the Green Bay, Wisconsin area, thereby providing a population with potential PCB exposure from Lake Michigan sport fish consumption. All women with positive pregnancy tests from two Green Bay prenatal clinics were asked to complete a self-administered questionnaire on fish consumption, health and reproductive history, and other relevant issues and to provide blood samples for PCB analysis. A positive correlation was found between the amount of Lake Michigan fish mothers claimed to consume and their PCB serum levels. After the pregnancy period, reproductive outcome measures (fetal wastage; stillbirths; and birth weight, birth length, head circumference, ponderal index, and birth weight percentiles for live births) were abstracted from hospital labor reports. Typical negative associations between birth size measures and consumption of caffeine, smoking, and alcohol were found. Birth size was positively associated with gestational age, birth order, weight gain during pregnancy, male babies, and rural residence. Birth size was also associated with PCB exposure; however contrary to expectations, a positive association was found (P < 0.044) for most mothers (the exception being those mothers who gained more than 34 lb during their pregnancy). PCB exposures were lower than the other studies that found that birth size was negatively associated with PCB exposure.

Sport fish consumption and body burden levels of chlorinated hydrocarbons: a study of Wisconsin anglers.

Sport-caught fish consumption is the major source of polychlorinated biphenyls (PCBs) exposure for the general population. To assess this and 2,2'-bis-(p-chlorophenyl)-1,1-dichloroethylene (DDE) exposure, we surveyed 801 Wisconsin anglers for fishing and consumption habits and comprehension of and compliance with the Wisconsin fish consumption health advisory. The mean annual number of sport-caught fish meals was 18. Seventy-two percent of anglers were familiar with the health advisory and 57% had changed their fishing or fish consumption habits as a result of the advisory. The mean PCB serum congener sum level for 192 anglers was 2.2 micrograms/l (range = nondetectable to 27.1 micrograms/l); mean DDE was 6.3 micrograms/l (range = nondetectable to 40.0 micrograms/l). Statistically significant positive Spearman correlations were observed between sport-caught fish meals and PCB and DDE sera levels (R = .21 and .14, respectively) and between kilograms of fish caught and PCB sera levels (R = .25). These results demonstrate that anglers may provide a population for assessment of PCBs and DDE associated morbidity and mortality.

Is It Safe to Drink Water From Tennis Ball Containers?

In brief: Three brands of plastic tennis ball containers were tested to determine whether they were safe receptacles for drinking water. The containers were cultured for bacteria and viruses immediately after they were opened. Then they were filled with organic-free water; water samples were analyzed for organic compounds immediately after they were filled, every 30 minutes for two hours, and at 20 hours. Culture results showed that the containers were sterile, but chemical analyses of the water samples yielded low levels of toluene, trichloroethane, and thiocyanic acid. Although the amounts of these compounds were not toxic, the authors recommend that tennis players rinse the containers thoroughly at least once before using them to hold drinking water.