The use of low-power laser irradiation for faster vascularization of tissue transplants.

We studied the effect of low-power laser irradiation on vascularization and take of transplanted rabbit renal and pancreatic tissue in athymic nude mice. The mean size of the transplant and the number of blood vessels in it were higher in irradiated mice compared to nonirradiated controls. Moreover, the organ-specific structure of the transplants was preserved in irradiated mice, but not in the control group. These findings suggest that low-power laser irradiation can be used for promotion of vascularization and take of tissue transplants.

Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine.

Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.

Elliptical versus circular erythrocyte marginal bands: isolation, shape conversion, and mechanical properties.

Differentiation of nucleated erythrocytes involves transformation from spheroids to flattened discoids to mature flattened ellipsoids. The marginal band (MB) of microtubules is required for this process and continues to play a role in maintaining mature ellipsoidal cell shape. One hypothesis for MB function is that cell ellipticity is generated and maintained by asymmetric application of force across a flexible, circular MB frame by the membrane skeleton or other transverse elements. This is based on an earlier finding that isolated erythrocyte MBs are much more circular than MBs in situ. However, our present studies of salamander erythrocyte MBs isolated by a detergent-based method challenge this hypothesis. Most of these isolated MBs are initially elliptical, even though they lack transverse material (= E-MBs). They can be stabilized in that form for long periods and can be converted experimentally into the circular form (= C-MBs) by extended incubation in isolation medium or by treatment with elastase or subtilisin. We have tested an alternative hypothesis for generation and maintenance of ellipsoidal MBs, one based on intrinsic differential bending resistance and supported by construction of models. Using laser microsurgical transection to compare mechanical responses of isolated E-MBs and C-MBs, we have found their behavior to be quite different. Whereas C-MBs linearize, most E-MBs do not, instead retaining considerable curvature. These results are incompatible with the differential bending resistance hypothesis, which predicts both C-MB and E-MB linearization. However, they are consistent with a third model, in which material bound to the MB stabilizes it in the mature ellipsoidal form, and indicate that the mechanism for maintenance of MB ellipticity differs from that involved in its generation.