[Study of prevalence of rare and difficult to cultivate causative agents of inflammatory diseases of respiratory organs].

AIM: Study the prevalence of Mycoplasma pneumoniae, Chlamydophila pneumoniae, Chlamydophila psittaci, Legionella pneumophila, Moraxella catarrhalis, Cytomegalovirus, Herpes simplex I/II virus (HSV I/II) in individuals of various age groups with varying inflammatory broncho-pulmonary diseases. MATERIALS AND METHODS: 384 adults and 1001 children with clinically confirmed diagnoses were examined by PCR method: community-acquired pneumonia, acute bronchitis, bronchial asthma, ARD/ARVD, as well as 127 healthy children and 52 healthy adults. Sputum, smears from posterior fornix of pharynx, blood, saliva from children of the first year of life were used as material for the study. RESULTS: Wide prevalence of M. pneumoniae and C. pneumoniae among adults and M. pneumoniae among children older than 1 year with inflammatory diseases of respiratory organs was established. C. psittaci, L. pneumophila, M. catarrhalis occurred in isolated cases in both adults and children. Active replication of herpes group viruses was detected in patients of all age groups with inflammatory broncho-pulmonary diseases, and in children Cytomegalovirus replication predominated, in adults--HSV I/II. CONCLUSION: High frequency of prevalence of M. pneumoniae and C. pneumoniae in inflammatory diseases of respiratory tract was established, giving evidence of reasonability and necessity of examination of patients with various nosologic forms of diseases for these species of microorganisms with the aim of effective etiotropic therapy.

[Electrical resistance of bacterial culture suspension as indicator of epidemic and sporadic variants of Shigella].

For the first time method of measurement of electric resistance of bacterial suspension was used for differentiation of "epidemic" and "sporadic" (further used without quotes) variants of Shigella species. Study of 40 strains of S. flexneri and S. sonnei revealed significant differences in electric resistance of cultures isolated during epidemic outbreaks after the growth during 1 or 6 hours - 18.1+/-8.3 kOhm and 84.6+/-7.8 kOhm respectively (increase in 4.9-fold; p<0.01), while non significant differences were observed in cultures isolated from sporadic cases after the growth during 1 or 6 hours - 21.1+/-9.3 and 57.1+/-11.3 kOhm respectively (increase in 2.5-fold; p>0.05). Express-method for differentiation of epidemic and sporadic variants of Shigella can be useful for timely detection of outbreaks beginning (preoutbreak).