RESUMEN
Products of arachidonic acid metabolism can influence normal and malignant cell growth. In vivo, inhibitors of arachidonic acid metabolism have been associated with inhibition of tumor growth, including head and neck squamous cell carcinoma (HNSCC). This has not been evaluated extensively in vitro in an HNSCC model. Therefore we investigated the effects of several arachidonic acid cascade inhibitors (AACIs) (indomethacin, curcumin, phenidone, nordihydroguaiaretic acid, 5,8,11,14-eicosatetraynoic acid, and 13-cisretinoic acid) on the growth of two HNSCC cell lines (MDA 886Ln and 1483). We found that AACIs caused dose-dependent growth inhibition of both cell lines. In an effort to inhibit HNSCC cell growth at lower concentrations of these drugs, we evaluated the effects of a variety of AACIs in combination with 13-cis retinoic acid. We observed synergistic growth inhibition when the drugs were used in all combinations, with the exception of indomethacin. These results suggest that AACIs may have some utility in the direct treatment of HNSCC, and a strategy combining 13-cis retinoic acid with other AACIs may prove to be even more effective.
Asunto(s)
Ácido Araquidónico/farmacología , Carcinoma de Células Escamosas/patología , Inhibidores de la Ciclooxigenasa/farmacología , Neoplasias de Cabeza y Cuello/patología , Tretinoina/farmacología , División Celular/efectos de los fármacos , Sinergismo Farmacológico , Humanos , Técnicas para Inmunoenzimas/instrumentación , Prostaglandinas E/metabolismo , Células Tumorales Cultivadas/efectos de los fármacosAsunto(s)
Otitis Media/complicaciones , Infecciones Estreptocócicas/complicaciones , Streptococcus pyogenes , Absceso/etiología , Enfermedad Aguda , Adulto , Anciano , Antibacterianos/uso terapéutico , Espacio Epidural , Femenino , Humanos , Enfermedades del Laberinto/etiología , Meningitis Bacterianas/etiología , Otitis Media/tratamiento farmacológico , Otitis Media/microbiología , Canales Semicirculares , Enfermedades de la Columna Vertebral/etiología , Infecciones Estreptocócicas/tratamiento farmacológicoAsunto(s)
Colesteatoma/diagnóstico , Enfermedades Cocleares/diagnóstico , Enfermedades Vestibulares/diagnóstico , Colesteatoma/congénito , Colesteatoma/patología , Enfermedades Cocleares/congénito , Enfermedades Cocleares/patología , Fibrosis , Humanos , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Hueso Petroso/patología , Enfermedades Vestibulares/congénito , Enfermedades Vestibulares/patologíaRESUMEN
Auditory brainstem responses (ABRs) were recorded from 18 neonates born to mothers who used cocaine during pregnancy, and from 18 control infants matched to the cocaine group on the basis of birthweight and conceptional age. ABRs were elicited using click signals presented at 40, 60, and 80 dB nHL at a rate of 33 clicks/sec and also at 11 and 89 clicks/sec at 80 dB nHL. The absolute latencies for ABR components I, III, and V and wave I-V interpeak latencies (IPLs) were measured for each signal condition.ABR absolute and IPLs for the infants who had been exposed to cocaine were prolonged relative to their age- and weight-matched controls. Neurodevelopmental compromise of the auditory system appears to result from gestational exposure to cocaine.
