RESUMEN
The basis of drug resistance in human epilepsy is not understood. Parallels with resistance in cancer suggest that drug resistance proteins may have a role. To examine this possibility, we have studied human brain tissue containing pathologies capable of causing refractory epilepsy. Using immunohistochemistry for P glycoprotein (Pgp) and multidrug resistance-associated protein 1 (MRP1), we examined both pathological tissue and control tissue. We demonstrate expression of Pgp and MRP1 in glia from cases of malformation of cortical development studied both before and after the onset of epilepsy, as well as in cases of hippocampal sclerosis and dysembryoplastic neuroepithelial tumours. In one particular type of malformation, we also demonstrate that dysplastic neurons express MRP1. The pattern of immunolabelling suggests overexpression is concentrated particularly around vessels in most of the pathologies. The timing shows that expression may be constitutive in some pathologies. These findings suggest that drug resistance proteins may contribute to drug resistance in refractory epilepsy.
Asunto(s)
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/análisis , Anticonvulsivantes/uso terapéutico , Química Encefálica , Epilepsia/tratamiento farmacológico , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/análisis , Proteínas del Tejido Nervioso/análisis , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/fisiología , Anticuerpos Monoclonales/inmunología , Anticonvulsivantes/farmacología , Barrera Hematoencefálica , Neoplasias Encefálicas/química , Neoplasias Encefálicas/patología , Corteza Cerebral/anomalías , Resistencia a Medicamentos/fisiología , Resistencia a Múltiples Medicamentos , Epilepsias Parciales/tratamiento farmacológico , Epilepsias Parciales/metabolismo , Epilepsias Parciales/patología , Epilepsia/metabolismo , Epilepsia/patología , Hipocampo/patología , Humanos , Inmunohistoquímica , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/fisiología , Proteínas de Neoplasias/análisis , Proteínas del Tejido Nervioso/fisiología , Tumores Neuroectodérmicos Primitivos/química , Tumores Neuroectodérmicos Primitivos/patología , Neuroglía/metabolismo , EsclerosisRESUMEN
Epilepsy is resistant to drug treatment in about one-third of cases, but the mechanisms underlying this drug resistance are not understood. In cancer, drug resistance has been studied extensively. Amongst the various resistance mechanisms, overexpression of drug resistance proteins, such as multi-drug resistance gene-1 P-glycoprotein (MDR1) and multidrug resistance-associated protein 1 (MRP1), has been shown to correlate with cellular resistance to anticancer drugs. Previous studies in human epilepsy have shown that MDR1 and MRP1 may also be overexpressed in brain tissue from patients with refractory epilepsy; expression has been shown in glia and neurones, which do not normally express these proteins. We examined expression of MDR1 and MRP1 in refractory epilepsy from three common causes, dysembryoplastic neuroepithelial tumours (DNTs; eight cases), focal cortical dysplasia (FCD; 14 cases) and hippocampal sclerosis (HS; eight cases). Expression was studied immunohistochemically in lesional tissue from therapeutic resections and compared with expression in histologically normal adjacent tissue. With the most sensitive antibodies, in all eight DNT cases, reactive astrocytes within tumour nodules expressed MDR1 and MRP1. In five of eight HS cases, reactive astrocytes within the gliotic hippocampus expressed MDR1 and MRP1. Of 14 cases of FCD, MDR1 and MRP1 expression was noted in reactive astrocytes in all cases. In five FCD cases, MRP1 expression was also noted in dysplastic neurones. In FCD and DNTs, accentuation of reactivity was noted around lesional vessels. Immunoreactivity was always more frequent and intense in lesional reactive astrocytes than in glial fibrillary acidic protein-positive reactive astrocytes in adjacent histologically normal tissue. MDR1 is able to transport some antiepileptic drugs (AEDs), and MRP1 may also do so. The overexpression of these drug resistance proteins in tissue from patients with refractory epilepsy suggests one possible mechanism for drug resistance in patients with these pathologies. We propose that overexpressed resistance proteins lower the interstitial concentration of AEDs in the vicinity of the epileptogenic pathology and thereby render the epilepsy caused by these pathologies resistant to treatment with AEDs.
Asunto(s)
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Anticonvulsivantes/uso terapéutico , Encéfalo/metabolismo , Epilepsia/metabolismo , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/metabolismo , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/genética , Anticonvulsivantes/metabolismo , Encéfalo/patología , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patología , Resistencia a Medicamentos , Epilepsia/tratamiento farmacológico , Epilepsia/etiología , Epilepsia/genética , Humanos , Inmunohistoquímica , Proteínas Asociadas a Resistencia a Múltiples Medicamentos/genética , Neoplasias Neuroepiteliales/metabolismo , Neoplasias Neuroepiteliales/patología , Neuroglía/metabolismo , Neuroglía/patología , Neuronas/metabolismo , Neuronas/patología , Esclerosis/patologíaRESUMEN
The term apoptosis refers to a peculiar morphology of cell death. It is of special interest because it can be triggered physiologically (and pathologically), and it is regulated by the actions of specific gene products. Therefore, it can in principle be activated and suppressed by medical intervention. It thus is often important to determine whether cells are dying by apoptosis (or its less regulated counterpart, necrosis) and also to quantity the effect in a population of cells. Here the classic methods of apoptosis quantitation are described; they will be of particular use to those whose laboratories are set up for standard microscopical and biochemical techniques, who do apoptosis assays infrequently but wish them to be widely accepted and reproducible. A simple microscopic observation, using blue light illumination and a pair of fluorescent dyes, is recommended for most applications.
Asunto(s)
Apoptosis , Electroforesis en Gel de Agar , HumanosRESUMEN
Cajal-Retzius (CR) cells are early-developing cells important in mammalian corticogenesis. Reelin, a protein secreted by CR cells, is essential for completion of neuronal migration and cortical lamination. Lack of reelin causes the 'reeler' phenotype in mice and autosomal recessive lissencephaly with cerebellar hypoplasia in man. Focal increases in reelin and CR cells are associated with thickening and local invaginations of the marginal zone and microgyria in animal studies. It has been suggested that abnormalities of reelin expression may be involved in human polymicrogyria. We have studied CR cells and reelin expression in pathological sections of human polymicrogyria to explore this possibility. Occurrence, distribution, morphology and reelin expression in CR cells were studied in 12 cases of human polymicrogyria, ranging from 21 gestational weeks to 10 years of age. Findings were compared with age-matched controls. Large, reelin-positive CR-like cells were more numerous in the majority of the polymicrogyria cases and persisted for longer than usual, up to 10 years of age. The CR-like cells tended to cluster and were most frequent in fused molecular layers in the polymicrogyria. Reelin-expressing CR-like cells were also found in bridges between the molecular layer and overlying leptomeningeal heterotopia and within the heterotopia itself. Clusters of CR-like cells were also found in adjacent non-polymicrogyric cortex. No clusters were seen in the control subjects. Increased numbers of CR-like cells were seen in both familial and acquired cases. In contrast to previous reports, the findings show that large CR-like cells persisted for longer than usual, up to 10 years of age, and that they may continue to express reelin. Their maximal aggregation in regions of polymicrogyria and overlying leptomeningeal heterotopia suggest an association between the presence of these cells and polymicrogyria, which we interpret in the light of recent findings concerning the roles of reelin and its downstream signalling pathway in neuronal and glial developmental dynamics and post-developmental function.
Asunto(s)
Astrocitos/metabolismo , Encéfalo/anomalías , Encéfalo/metabolismo , Moléculas de Adhesión Celular Neuronal/biosíntesis , Proteínas de la Matriz Extracelular/biosíntesis , Malformaciones del Sistema Nervioso/metabolismo , Envejecimiento/metabolismo , Envejecimiento/patología , Astrocitos/patología , Encéfalo/patología , Calbindina 2 , Recuento de Células , Niño , Preescolar , Coristoma/patología , Proteína Ácida Fibrilar de la Glía/biosíntesis , Humanos , Lactante , Recién Nacido , Meninges/patología , Proteínas del Tejido Nervioso , Malformaciones del Sistema Nervioso/patología , Neuronas/patología , Proteína Reelina , Reticulina/biosíntesis , Proteína G de Unión al Calcio S100/biosíntesis , Serina EndopeptidasasRESUMEN
Drug resistance in epilepsy is poorly understood. We used routine immunohistochemistry to assess overexpression of a multidrug-resistance protein in dysplastic neurons, glia, and around vessels in surgically resected epileptogenic human brain tissue. We showed non-tumoral overexpression of this multidrug-resistance protein, which might contribute to drug resistance in epilepsy caused by focal cortical dysplasia.
Asunto(s)
Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/análisis , Corteza Cerebral/química , Epilepsia/metabolismo , Corteza Cerebral/patología , Epilepsia/patología , Humanos , InmunohistoquímicaRESUMEN
This paper describes the neuroimaging and neuropathological findings in five cases of severe brain damage after traumatic mid-trimester amniocentesis, all performed between 1986 and 1994. Although fetal injury after amniocentesis has been reported, reports of brain injury are infrequent. Continuous ultrasound monitoring may reduce the risk of fetal injury but follow-up ultrasound scans can be falsely reassuring. Withdrawal of blood-stained fluid, particularly if it contains tissue fragments, should alert the operator to the possibility of fetal damage. Histological examination of such tissue fragments may confirm the nature of the fetal damage. The consequences of fetal brain injury are severe, all five of our cases showed evidence of disruption of brain development compatible with mid-term injury. Obstetricians and their patients should be aware of the small but significant risk of brain damage after mid-term amniocentesis.
Asunto(s)
Amniocentesis/efectos adversos , Lesiones Encefálicas/etiología , Complicaciones del Embarazo , Ultrasonografía Intervencional , Adulto , Amniocentesis/métodos , Encéfalo/crecimiento & desarrollo , Reacciones Falso Negativas , Femenino , Humanos , Recién Nacido , Persona de Mediana Edad , Embarazo , Segundo Trimestre del Embarazo , Estudios Retrospectivos , Medición de RiesgoRESUMEN
NMDA receptors (NR) are important in many neurological processes. Using a large series of human brain tissue, we show that the distribution of NR2A/B immunoreactivity varies according to position along a gyrus. For pyramidal neurons in laminae II and III, immunoreactivity is most marked at gyral crown and gyral lips, diminishes along sulcal wall, and is barely detectable in sulcal floor cortex. In contrast, in some cases, immunoreactivity in laminae V and VI pyramidal neurons shows the reverse pattern. Neurofilament and calretinin immunoreactivity do not show this phenomenon. The findings suggest novel functional regionalization at the sulcogyral level in normal human brain.
Asunto(s)
Corteza Cerebral/citología , Corteza Cerebral/metabolismo , Células Piramidales/citología , Células Piramidales/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Adolescente , Adulto , Calbindina 2 , Corteza Cerebral/anomalías , Niño , Preescolar , Femenino , Humanos , Lactante , Masculino , Proteínas de Neurofilamentos/metabolismo , Proteína G de Unión al Calcio S100/metabolismoRESUMEN
Drug resistance in epilepsy due to malformations of cortical development (MCD) is unexplained. P-glycoprotein is a mediator of drug resistance, and we propose that MCD lesions over-express P-glycoprotein. Because P-glycoprotein expression may be induced by some antiepileptic drugs (AEDs), we studied brain samples from MCD cases before the onset of seizures or treatment with AEDs. Sixteen MCD cases and 16 age-matched controls were examined using immunohistochemistry. Glial labelling, representing over-expression, was seen in 10 of 16 MCD samples and in two of 16 control samples (p = 0.003). Semiquantitative assessment showed many immunoreactive glia in five of 16 MCD and one of 16 controls. We conclude that there is constitutive over-expression of P-glycoprotein in many MCD.
Asunto(s)
Subfamilia B de Transportador de Casetes de Unión a ATP/biosíntesis , Química Encefálica/fisiología , Corteza Cerebral/anomalías , Corteza Cerebral/patología , Recuento de Células , Corteza Cerebral/metabolismo , Niño , Preescolar , Epilepsia/metabolismo , Epilepsia/patología , Humanos , Inmunohistoquímica , Lactante , Recién Nacido , Neuroglía/metabolismoRESUMEN
Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal cortical dysplasia and intractable epilepsy, and one dysmorphic term infant with associated hydrocephalus and polymicrogyria. The connectivity of nodules was investigated using histological and carbocyanine dye (DiI) tracing techniques. DiI crystal placement adjacent to heterotopic nodules revealed numerous DiI-labelled fibres within a 2-3 mm radius of the crystals. Although we observed labelled fibres closely surrounding nodules, the majority did not penetrate them. Placement of DiI crystals within nodules also identified a limited number of projections out of the nodules and in one case there was evidence for connectivity between adjacent nodules. The cellular and neurochemical composition of nodules was also examined using immunohistochemistry for calretinin and neuropeptide Y (NPY), which are normally expressed in GABAergic cortical interneurons. Within heterotopic nodules from all cases, numerous calretinin-positive neurons were identified, along with a few cell bodies and many processes positive for NPY. Calretinin-positive neurons within nodules were less morphologically complex than those in the cortex, which may reflect incomplete differentiation into an inhibitory neuronal phenotype. There were also abnormal clusters of calretinin-positive cells in the overlying cortical plate, indicating that the migratory defect which produces heterotopic nodules also affects development of the cortex itself. Thus, heterotopic nodules consisting of multiple neuronal cell types are associated with malformation in the overlying cortical plate, and have limited connectivity with other brain regions. This abnormal development of connectivity may affect neuronal maturation and consequently the balance of excitation and inhibition in neuronal circuits, leading to their epileptogenic potential.
Asunto(s)
Corteza Cerebral , Coristoma/diagnóstico , Epilepsia/diagnóstico , Química Encefálica , Calbindina 2 , Carbocianinas , Tamaño de la Célula , Niño , Coristoma/patología , Epilepsia/patología , Resultado Fatal , Femenino , Técnica del Anticuerpo Fluorescente , Colorantes Fluorescentes , Humanos , Recién Nacido , Interneuronas/química , Interneuronas/patología , Imagen por Resonancia Magnética , Masculino , Neuropéptido Y/análisis , Proteína G de Unión al Calcio S100/análisis , Ácido gamma-Aminobutírico/análisis , Ácido gamma-Aminobutírico/fisiologíaRESUMEN
The average polymorphonuclear neutrophil (PMN) lives only a day and then dies by apoptosis. We previously found that the calcium-dependent protease calpain is required for apoptosis in several mouse models of cell death. Here we identify calpain, and its endogenous inhibitor calpastatin, as regulators of human neutrophil apoptosis. Cell death triggered by the translation inhibitor cycloheximide is calpain-dependent, as evidenced using either a calpain active site inhibitor (N-acetyl-leucyl-leucyl-norleucinal) or agents that target calpain's calcium binding sites (PD150606, PD151746). No significant effect on cycloheximide-triggered apoptosis was found by using inhibitors of the proteasome or of other papain-like cysteine proteases, providing further evidence that the active site calpain inhibitor prevents apoptosis via its action on calpain. In addition, we find that potentiation of calpain activity by depleting its endogenous inhibitor, calpastatin, is sufficient to cause apoptosis of neutrophils. Nevertheless, apoptosis signalled via the Fas antigen proceeds regardless of the presence of calpain inhibitor. These experiments support a growing body of work, indicating an upstream regulatory role for calpain in many, but not all, forms of apoptotic cell death. They also identify calpastatin as a participant in apoptotic cell death and suggest that for at least one cell type, a decrease in calpastatin is a sufficient stimulus to initiate calpain-dependent apoptosis.
Asunto(s)
Acrilatos/farmacología , Apoptosis/fisiología , Proteínas de Unión al Calcio/sangre , Calpaína/sangre , Inhibidores de Cisteína Proteinasa/farmacología , Neutrófilos/fisiología , Adulto , Animales , Apoptosis/efectos de los fármacos , Proteínas de Unión al Calcio/genética , Calpaína/antagonistas & inhibidores , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Cicloheximida/farmacología , Inhibidores de Cisteína Proteinasa/sangre , Humanos , Cinética , Ratones , Neutrófilos/citología , Oligodesoxirribonucleótidos Antisentido/farmacología , Biosíntesis de Proteínas/efectos de los fármacos , ARN Mensajero/genética , Tionucleótidos , Transcripción Genética/efectos de los fármacosRESUMEN
The cellular pathways of apoptosis have not been fully characterized; however, calpain, a cytosolic calcium-activated cysteine protease, has been implicated in several forms of programmed cell death. Reoviruses induce apoptosis both in vitro and in vivo and serve as a model for studying virus-induced cell death. We investigated the potential role of calpain in reovirus-induced apoptosis in vitro by measuring calpain activity as well as evaluating the effects of calpain inhibitors. L929 cells were infected with reovirus type 3 Abney (T3A), and calpain activity, measured as cleavage of the fluorogenic calpain substrate Suc-Leu-Leu-Val-Tyr-AMC, was monitored. There was a 1.6-fold increase in calpain activity in T3A-infected cells compared to mock-infected cells; this increase was completely inhibited by preincubation with calpain inhibitor I (N-acetyl-leucyl-leucyl-norleucinal [aLLN]), an active-site inhibitor. Both aLLN and PD150606, a specific calpain inhibitor that interacts with the calcium-binding site, inhibited reovirus-induced apoptosis in L929 cells by 54 to 93%. Apoptosis induced by UV-inactivated reovirus was also reduced 65 to 69% by aLLN, indicating that inhibition of apoptosis by calpain inhibitors is independent of effects on viral replication. We conclude that calpain activation is a component of the regulatory cascade in reovirus-induced apoptosis.
Asunto(s)
Apoptosis/efectos de los fármacos , Calpaína/fisiología , Inhibidores de Cisteína Proteinasa/farmacología , Reoviridae/fisiología , Acrilatos/farmacología , Animales , Calpaína/antagonistas & inhibidores , Inhibidores de Cisteína Proteinasa/uso terapéutico , Ratones , Transducción de Señal , Rayos UltravioletaRESUMEN
Butylated hydroxytoluene (BHT) causes lung injury in mice and promotes tumor formation. Hydroxylation of a tert-butyl group on BHT to yield the metabolite, 6-tert-butyl-2-[2'-(2'-hydroxymethyl)-propyl]-4-methylphenol (BHTOH), may be required. BHTOH is more potent than BHT on an equimolar basis in causing lung damage, enhancing lung tumor development, killing isolated bronchiolar non-ciliated Clara cells, and inhibiting lung epithelial gap junctional intercellular communication. One mechanism proposed for tumor promoting agents is selective cytotoxicity; killing normal cells allows uninhibited clonal expansion of neighboring initiated cells. We compared the abilities of BHT, BHTOH, and other BHT metabolites to kill non-tumorigenic and tumorigenic mouse and human lung cell lines, and examined the contribution of apoptosis to this cytotoxicity. These cells lack the cytochrome P450 2B isozyme necessary for converting BHT to BHTOH. BHTOH and 4-hydroperoxy-4-methyl-2,6-di-tert-butyl-2,5-cyclohex-adienone+ ++ (BHTOOH) were most toxic, BHT and 2,6-di-tert-butyl-1,4-benzoquinone (BHTQu) were less potent, and 4-methyl BHT metabolites that are not pneumotoxic were ineffective. BHTOH most strongly induced apoptosis, based on nuclear condensation and transmission electron microscopy. Non-tumorigenic cells were as susceptible to cell death as the neoplastic cell lines when apoptosis and necrosis are not distinguished, but more sensitive to BHTOH-induced apoptosis. An apoptotic mechanism may underlie the lung tumor promoting actions of BHTOH.
Asunto(s)
Apoptosis/efectos de los fármacos , Hidroxitolueno Butilado/análogos & derivados , Carcinógenos/toxicidad , Transformación Celular Neoplásica/efectos de los fármacos , Células Epiteliales/efectos de los fármacos , Pulmón/efectos de los fármacos , Animales , Bronquios/citología , Bronquios/efectos de los fármacos , Hidroxitolueno Butilado/química , Hidroxitolueno Butilado/toxicidad , Recuento de Células , Núcleo Celular/efectos de los fármacos , Núcleo Celular/ultraestructura , Transformación Celular Neoplásica/patología , Citocromo P-450 CYP2B1/deficiencia , Citocromo P-450 CYP2B1/metabolismo , Células Epiteliales/enzimología , Células Epiteliales/patología , Humanos , Pulmón/enzimología , Pulmón/patología , Neoplasias Pulmonares , Ratones , Ratones Endogámicos , Células Tumorales CultivadasRESUMEN
Magnetic resonance imaging studies in two cases of neonatal hypoglycaemia showed cortical and white matter cerebral damage that was most obvious in the occipital lobes. Both cases showed oedema in the parieto-occipital cortex and underlying white matter in the acute phase, with profound atrophy of these regions in the chronic phase. These findings support those of pathological studies which suggest that hypoglycaemia induces cerebral damage by a mechanism separate from the effects of cerebral hypoxia-ischaemia caused by secondary seizures.
Asunto(s)
Encefalopatías/etiología , Encéfalo/diagnóstico por imagen , Hipoglucemia/complicaciones , Encéfalo/patología , Encefalopatías/diagnóstico por imagen , Encefalopatías/patología , Niño , Humanos , Hipoglucemia/diagnóstico por imagen , Hipoglucemia/patología , Lactante , Recién Nacido , Imagen por Resonancia Magnética , Masculino , Tomografía Computarizada por Rayos XRESUMEN
Sporadic inclusion body myositis (s-IBM) is a chronic inflammatory myopathy of unknown pathogenesis. The common findings of ragged red fibres, cytochrome c oxidase-negative fibres and multiple mitochondrial DNA deletions in the muscle of patients with s-IBM have suggested that a deficit of energy metabolism may be of pathogenic relevance. To test this hypothesis we used 31P magnetic resonance spectroscopy to assess in vivo skeletal muscle mitochondrial function in the calf muscles of 12 patients with definite s-IBM. Eleven patients showed multiple mitochondrial DNA deletions in skeletal muscle and 67% showed ragged red fibres and/or cytochrome c oxidase-negative fibres. T1-weighted MR images showed increased signal intensity in the calf muscle of all patients except one. The involvement of calf muscle was confirmed by 31P magnetic resonance spectroscopy of resting muscle, which disclosed abnormalities in metabolite ratios in all patients. However, muscle oxidative metabolism assessed during recovery from exercise was normal in patients with s-IBM, as maximum rates of mitochondrial ATP production and post-exercise ADP recovery rates were within the normal range in all cases. We conclude that muscle mitochondrial abnormalities are a secondary process and unlikely to play a significant role in the pathogenesis of s-IBM.
Asunto(s)
Metabolismo Energético , Músculo Esquelético/metabolismo , Miositis por Cuerpos de Inclusión/metabolismo , Adenosina Difosfato/metabolismo , Adenosina Trifosfato/metabolismo , Adulto , Edad de Inicio , Anciano , ADN Mitocondrial/genética , Femenino , Humanos , Imagen por Resonancia Magnética , Espectroscopía de Resonancia Magnética , Masculino , Persona de Mediana Edad , Mitocondrias Musculares/genética , Mitocondrias Musculares/metabolismo , Músculo Esquelético/fisiopatología , Miositis por Cuerpos de Inclusión/genética , Miositis por Cuerpos de Inclusión/fisiopatología , Consumo de Oxígeno , Fosfatos/metabolismo , Fosfocreatina/metabolismo , Fósforo , Esfuerzo Físico/fisiología , Valores de Referencia , Eliminación de SecuenciaRESUMEN
This study investigated whether both mild and severe hypoxia-ischaemia (HI) caused significant numbers of cells to die by apoptosis in the developing brain in vivo. Newborn piglets were subjected to transient global HI and the fraction of all cells in the cingulate gyrus that were apoptotic or necrotic counted 48 h after resuscitation. The mean (S.D.) proportion of apoptotic cells was 11.9% (6.7%) (sham operated controls 4.1% (2.7%)), while 11.4% (8.4%) were necrotic (controls 0.7% (1.3%)) (P<0.05). Apoptotic and necrotic cell counts were both linearly related to the severity of impaired cerebral energy metabolism measured by magnetic resonance spectroscopy (P<0.05), as shown by: (1) the decline in the ratio of nucleotide triphosphates to the exchangeable phosphate pool during HI; (2) the fall in the ratio of phosphocreatine to inorganic phosphate 8 - 48 h after HI; and (3) an increased ratio of lactate to total creatine at both these times. Thus both apoptosis and necrosis occurred in the cingulate gyrus after both severe and mild HI in vivo in proportion to the severity of the insult.
Asunto(s)
Apoptosis/fisiología , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patología , Metabolismo Energético , Hipoxia Encefálica/metabolismo , Hipoxia Encefálica/patología , Animales , Animales Recién Nacidos , Encéfalo/metabolismo , Recuento de Células , Giro del Cíngulo/patología , Ácido Láctico/metabolismo , Espectroscopía de Resonancia Magnética , Necrosis , Fosfocreatina/metabolismo , PorcinosRESUMEN
This study addressed the hypothesis that in human infants severe in utero insults induce a significant proportion of brain cells to undergo apoptosis. Morphologic criteria were used to quantify apoptosis and necrosis in the cingulate gyrus of two groups of infants: six infants who died after severe birth asphyxia with hypoxic-ischemic encephalopathy, and six others who suffered unexpected and apparently sudden intrauterine death at or close to term. The fraction of apoptotic cells was much higher than basal levels determined in animal experiments, and within both groups increased in proportion to the severity of injury as determined by total cell death (p < 0.05). The mean fraction of apoptotic cells was similar in asphyxiated infants, 8.3% (95% confidence interval for the population, 3.7-12%), and in stillbirths, 6.7% (0.2-13.6%). In the asphyxiated group, 20.8% (11-30.6%) of cells were necrotic, but significantly less necrosis, 3% (0.4-5.6%), was seen in stillborn infants (p < 0.05). Cell death was apoptotic after birth asphyxia in 26% (1-51%) and 78% (41-100%) in stillborn infants. In situ end labeling studies confirmed the presence of DNA fragmentation in apoptotic cells. These results demonstrate that infants who die after intrauterine insults, both those with evidence of delayed cerebral injury after hypoxia-ischemia and those without, have a significant number of cells in the brain with the morphologic characteristics of apoptosis. They confirm that apoptosis contributes significantly to cerebral damage in the perinatal period.
Asunto(s)
Apoptosis , Lesiones Encefálicas/patología , Isquemia Encefálica/patología , Muerte Fetal/patología , Hipoxia Encefálica/patología , Diagnóstico Prenatal , Femenino , Giro del Cíngulo/embriología , Giro del Cíngulo/patología , Humanos , Recién Nacido , Masculino , NecrosisRESUMEN
Mutations in the genes encoding dystrophin or dystrophin-associated proteins are responsible for Duchenne muscular dystrophy or various forms of limb-girdle muscular dystrophies respectively. We have recently cloned the gene for the murine 87 kDa postsynaptic protein dystrobrevin, a dystrophin-associated protein. Anti-dystrobrevin antibodies stain the sarcolemma in normal skeletal muscle indicating that dystrobrevin co-localises with dystrophin and the dystrophin-associated protein complex. By contrast, dystrobrevin membrane staining is severely reduced in muscles of Duchenne muscular dystrophy patients, consistent with dystrobrevin being a dystrophin-associated protein. Interestingly, dystrobrevin staining at the sarcolemma is dramatically reduced in patients with limb-girdle muscular dystrophy arising from the loss of one or all of the sarcoglycan components. Normal dystrobrevin staining is observed in patients with other forms of limb-girdle muscular dystrophy where dystrophin and the rest of the dystrophin-associated protein complex are normally expressed and in other neuromuscular disorders. Our results show that dystrobrevin-deficiency is a generic feature of dystrophies linked to dystrophin and the dystrophin-associated proteins. This is the first indication that a cytoplasmic component of the dystrophin-associated protein complex may be involved in the pathogenesis of limb-girdle muscular dystrophy.
Asunto(s)
Proteínas Asociadas a la Distrofina , Distrofias Musculares/genética , Neuropéptidos/deficiencia , Sarcolema/metabolismo , Proteínas del Citoesqueleto/metabolismo , Distroglicanos , Distrofina/inmunología , Distrofina/metabolismo , Humanos , Glicoproteínas de Membrana/metabolismo , Músculo Esquelético/metabolismo , Neuropéptidos/genética , Neuropéptidos/metabolismo , SarcoglicanosRESUMEN
Mice deficient in MARCKS, a prominent neural substrate for protein kinase C (PKC), die before or shortly after birth. They exhibit high frequencies of exencephaly, universal agenesis of forebrain commissures, and abnormalities of cerebral cortical and retinal lamination. We show here that these mice have wide-spread and severe neuronal ectopia in the outer layers of the developing forebrain, manifested by the migration of clusters of developing neuroblasts through the basal lamina and often through the pial membrane and into the subarachnoid space. This abnormality became apparent by Embryonic Day (E) 13 or 14, shortly after the formation of the early marginal zone. MARCKS deficiency was associated with decreased staining for marginal zone chondroitin sulfate proteoglycans; this decrease was detectable earlier in development than the neuronal ectopia. Later in development, there was also marked disruption of the basal lamina at the pial-glial interface, as evidenced by gross abnormalities in laminin and reticulin staining; however, the basal lamina appeared normal at E9.5. These data indicate that MARCKS is required for the prevention of neuronal ectopia during development. Potential mechanisms responsible for the neuronal ectopia in the MARCKS-deficient mice include decreased expression or increased proteolytic destruction of basal lamina proteins and marginal zone chondroitin sulfate proteoglycans in the developing brain.