RESUMEN
It was found that, depending on their frequency, mechanical vibrations (MVs) can either stimulate (4 Hz) or inhibit (50 Hz) the growth and the division of the lon mutant of Escherichia coli K-12. Similar effects were observed when the MV-treated nutrient medium was inoculated with untreated mutant cells. MVs enhanced the motility of mutant cells and the fragmentation of filament cells always present in the populations of lon mutants.
Asunto(s)
Proteínas de Escherichia coli , Escherichia coli/fisiología , Proteasa La , Proteasas ATP-Dependientes , Regulación Bacteriana de la Expresión Génica , Proteínas de Choque Térmico/genética , Mutación , Serina Endopeptidasas/genética , Estrés MecánicoRESUMEN
The effect of mechanical vibrations (MV) electrical conductivity of water and optical density of aqueous DNA solution. Distilled water was treated with MV of several frequencies from 3 to 5000 Hz with an intensity of 90 dB for 30 minutes. Different sensitivities of water specific electrical conductivity (SEC) were determined (the value of distilled water SEC was 209 +/- 2 microS/m). The greatest decrease of SEC (by 15.7%) under the influence of MV was observed at the frequency of 4 Hz. There was no effect at frequencies higher than 100 Hz. The treatment of DNA water solutions with MV of frequencies 4 and 10 Hz decreased its optical density by 4.2 +/- 1.1 and 4.8 +/- 1.2% correspondingly in comparison with control. In cases of treatment with frequencies of 20 and 50 Hz no effect was observed. The mechanism of MV effect on water can be connected with the changes of system structural characteristics. It is confirmed by experiments with DNA solution, where the decrease of optical density (at 260 nm) under MV treatment is conditioned with the increase of the probability of hydrogen binding formation between the bases.
Asunto(s)
ADN/química , Sonido , Agua/química , Fenómenos Químicos , Química Física , Conductividad Eléctrica , Soluciones , Factores de TiempoRESUMEN
It was shown that the static magnetic field (SMF) and electromagnetic field (EMF) caused inhibition of the cell division in Escherichia coli K-12 lon mutant. The low-frequency EMF 4 Hz led to the 20% survival, but EMF at 50 Hz increased the survival of cells up to 53%. After exposure to magnetic field cells lost capacity for division and grow as filaments, unable to form the colonies on the solid media.
Asunto(s)
Escherichia coli/efectos de la radiación , Magnetismo , Mutación/efectos de la radiación , División Celular/efectos de la radiación , Campos Electromagnéticos , Escherichia coli/citología , Escherichia coli/crecimiento & desarrollo , Tolerancia a Radiación , Factores de TiempoRESUMEN
The effect of perfusate on the contractile activity of an isolated internally perfused heart of Helix pomatia was studied. The changes in heart activity induced by the switching of the perfusate stream were more pronounced in a potassium-free solution when the Na+, K(+)-pump was inactivated. It was found that the decrease in the amplitude of contractions of snail heart by acetylcholine (5.10(-9) M) depends on the treatment of perfusate (Ringer solution) by mechanical vibrations (4, 10, 20, and 50 Hz; 90 dB). In the solution treated with 4 Hz mechanical vibrations, the inhibiting effect of acetylcholine decreased. A similar effect was observed after inactivating the Na+, K(+)-pump by ouabain (10(-4) M). Upon treating the solution by 10, 20, and 50 Hz mechanical vibrations, these changes were not observed. Based on the data, it is suggested that the water medium of the cell can serve as a target through which mechanical vibration can affect the cascade of cell metabolic processes.
Asunto(s)
Corazón/fisiología , Contracción Miocárdica/fisiología , Acetilcolina/farmacología , Animales , Corazón/efectos de los fármacos , Caracoles Helix/fisiología , Técnicas In Vitro , Perfusión , Estimulación Física , ATPasa Intercambiadora de Sodio-Potasio/fisiologíaRESUMEN
Platelet G protein subunits (G alpha i2, G alpha q and Gbeta) were measured in 15 non-treated depressed patients (recurrent major depression) and 15 age- and sex-matched healthy controls by using the Western immunoblot method. The depression severity was measured by the AMDP depression rating scale before start of treatment. The AMDP score ranged between 12 and 44. Patients were then treated with different antidepressant drugs (ATD) for 1 month, after which G protein and depression were reassessed. Results indicated that drug-free depressed patients displayed increased levels of G proteins subunits, in comparison to healthy controls. Antidepressant drug administration resulted in decrease of depression severity but only seven patients showed a net response to drugs (AMDP depression score less than 12). These drug-responding patients have also reduced G protein levels, while patients without significant improvement continued to display either the same levels of G proteins or higher, whatever the class of the drug administered. These results suggest that depression is associated to increase in G protein subunit levels and that the clinical outcome seemed to be the determining factor in further decrease occurring in G protein levels.
Asunto(s)
Plaquetas/metabolismo , Trastorno Depresivo/sangre , Proteínas de Unión al GTP/sangre , Adulto , Antidepresivos de Segunda Generación/uso terapéutico , Antidepresivos Tricíclicos/uso terapéutico , Western Blotting , Clomipramina/uso terapéutico , Trastorno Depresivo/tratamiento farmacológico , Trastorno Depresivo/psicología , Femenino , Humanos , Imipramina/uso terapéutico , Masculino , Persona de Mediana Edad , Paroxetina/uso terapéutico , Escalas de Valoración Psiquiátrica , Transducción de Señal/efectos de los fármacos , Resultado del TratamientoRESUMEN
In previous reports, we have observed that blood magnesium was significantly higher in drug-free patients with major depression when compared to healthy controls. This was especially true for erythrocyte magnesium. Furthermore, the most severely depressed patients had the highest intracellular magnesium content, showing that intracellular magnesium rate was related to the intensity of symptoms. We report here the results of blood magnesium measured in 88 major depressed patients as compared to 61 controls. We show that the mean erythrocyte and also plasma magnesium contents are both increased in these patients. We observe that about 40% of male and female patients have a very significant increase (25%) in intracellular magnesium content as compared to controls. However, about 60% of the hospitalised depressed patients have normal values. None of the controls has high erythrocyte magnesium. This is less evident concerning the plasma magnesium. No differences are observed between patients when classified according to the intensity of moral pain or anxiety. In contrast, the patients with mild to high psychomotor retardation score, which is an index of hypoexcitability, have significant higher erythrocyte magnesium values compared with other patients. The results of male patients without psychomotor retardation do not differ from control values. Our study suggests that central hypoexcitability might be related to an increase in intracellular magnesium observed at the peripheral level, keeping in mind that hyperexcitability, as observed in various conditions such as stress and cardiovascular disorders, is frequently associated, in contrast, with a decrease in blood magnesium.
RESUMEN
The cooling of hen incubated eggs to 28 degrees C brings to disconnection of oxidative phosphorylation in brain on the 12th day of development. The addition of DNP (5 x 10(-5) M) takes off the cooling effect.
Asunto(s)
Regulación de la Temperatura Corporal/fisiología , Encéfalo/fisiología , Frío , Fosforilación Oxidativa , 2,4-Dinitrofenol , Adenosina Trifosfatasas/efectos de los fármacos , Adenosina Trifosfatasas/metabolismo , Animales , Regulación de la Temperatura Corporal/efectos de los fármacos , Encéfalo/efectos de los fármacos , Encéfalo/embriología , Embrión de Pollo , Dinitrofenoles/farmacología , Mitocondrias/efectos de los fármacos , Mitocondrias/fisiología , Fosforilación Oxidativa/efectos de los fármacos , Desacopladores/farmacologíaRESUMEN
Studies have been made of the effects of short-term cooling of eggs at the second part of the incubation period on the intensity of oxidative phosphorylation and peroxidative oxidation of lipids in the mitochondria of skeletal muscles and heart from the developing chicks. It was shown that the intensity of respiration and phosphorylation increases in both tissues, being more significant in skeletal muscles. It is suggested that activation of peroxidative oxidation of lipids at the background of the increasing oxygen consumption and phosphate esterification during adaptation of animals to changes in the environment is one of the mechanisms accounting for the compensatory changes in fatty acid composition of lipids.
Asunto(s)
Pollos/metabolismo , Frío , Peroxidación de Lípido/fisiología , Músculos/metabolismo , Miocardio/metabolismo , Fosforilación Oxidativa , Animales , Embrión de Pollo , Malondialdehído/metabolismo , Mitocondrias Cardíacas/metabolismo , Mitocondrias Musculares/metabolismo , Factores de TiempoRESUMEN
The short-term cooling of hen eggs under incubation was studied for its effect on the dynamics of the activity of cytochrome oxidase (CO) and anion ATPase in the brain and liver of 15- and 20-day hen embryos and 5-day chickens. The temperature fall in the embryonal period was established to stimulate the activity of bicarbonate-dependent ATPase in the brain and to suppress it in the liver tissue. The CO activity was also subjected to similar alterations.
Asunto(s)
Adenosina Trifosfatasas/metabolismo , Pollos/crecimiento & desarrollo , Complejo IV de Transporte de Electrones/metabolismo , Temperatura , Animales , Encéfalo/enzimología , Embrión de Pollo , Mitocondrias/enzimología , Mitocondrias Hepáticas/enzimologíaRESUMEN
The effect of a short-term cooling of the incubated eggs has been investigated on the intensity of oxidative phosphorylation and the activity of ATPase in mitochondria from muscles and liver of chick embryos and chicks. It was found that the decrease of temperature increases oxygen consumption in muscle mitochondria decreasing esterification of inorganic phosphate. As a consequence, the value of P/O decreases. The activity of ATPase significantly increases. Uncoupling between oxidation and phosphorylation in liver mitochondria takes place more slowly. It is suggested that these changes account for realization of thermoregulation.
Asunto(s)
Regulación de la Temperatura Corporal , Embrión de Pollo/fisiología , Adenosina Trifosfatasas/metabolismo , Animales , Frío , Mitocondrias Hepáticas/enzimología , Mitocondrias Musculares/enzimología , Fosforilación Oxidativa , Consumo de Oxígeno , Factores de TiempoRESUMEN
When adding alpha-ketoglutarate and glutamate the intensity of respiration by the myocardium mitochondria increases gradually from the 15th day of embryonic development till the chicken hatching out. In the presence of succinate respiration of mitochondria of 15- and 20-day embryos and 5-day chickens is almost the same and decreases noticeably in adult chickens. When the above-mentioned substrates are added the value of P/O gradually decreases during the chicken development.
Asunto(s)
Mitocondrias Cardíacas/metabolismo , Fosforilación Oxidativa , Consumo de Oxígeno , Animales , Embrión de Pollo , Pollos , Glutamatos/metabolismo , Corazón/embriología , Ácidos Cetoglutáricos/metabolismoAsunto(s)
Médula Ósea/patología , Leucocitos , Convulsiones/sangre , Animales , Recuento de Leucocitos , Conejos , Convulsiones/patologíaAsunto(s)
Leucemia/epidemiología , Enfermedad Aguda , Adolescente , Adulto , Armenia , Niño , Preescolar , Enfermedad Crónica , Femenino , Humanos , Lactante , Recién Nacido , Masculino , Persona de Mediana Edad , Periodicidad , Población UrbanaRESUMEN
ATP-ase activity and the effect of cations (Na+, K+, Mg2+, Ca2+) and DNP on its activity have been studied in avian brain homogenates and mitochondrial and supernatant fractions (microsomes + hyaloplasma). Highest levels of Na+ + K+ -stimulated ATP-ase activity has been shown in avian brain crude mitochondrial fraction, next in supernatant and lowest in homogenates. Following three-fold freezing and thawing, enzyme activity in homogenates and mitochondrial fractions increases considerably and decreases in the supernatant. Highest Mg2+ -stimulated ATP-ase activity is found in avian brain homogenates, next in mitochondrial fractions and supernatants. Mg2+ -stimulated ATP-ase activity is not changed considerably following three-fold freezing and thawing of brain preparations. Highest Ca2+ -stimulated ATP-ase activity has been observed in the supernatant fraction and its activity is considerably reduced following freezing and thawing. Avian brain mitochondrial fractions have high DNP-stimulated ATP-ase activity. Following freezing and thawing of brain preparations DNP-stimulated ATP-ase activity is not changed considerably. The results obtained indicate that in avian brain homogenates and other preparations, cation and DNP-stimulated ATP-ase have different activities. Three-fold freezing and thawing of brain tissue preparations affect these activities differentially.