RESUMEN
BACKGROUND: Technetium-99m-sestamibi (sestamibi) is the imaging agent of choice for preoperative parathyroid localization. The subcellular localization of sestamibi uptake in enlarged parathyroid glands in patients with hyperparathyroidism has not been determined. This study investigated the mechanism of retention of sestamibi by human parathyroid tissue. METHODS: Twenty-three freshly harvested and 15 cryopreserved parathyroid glands excised from patients with primary or secondary hyperparathyroidism were analyzed for subcellular localization of Tc-99m-sestamibi. Tissues were incubated with 100 microCi of sestamibi and isolated for mitochondria by differential centrifugation, and the integrity of subcellular fractions was quantified with the mitochondrial enzyme marker, succinate dehydrogenase. RESULTS: Ninety-two percent of sestamibi activity was associated with mitochondria. Furthermore, after adding the mitochondrial uncoupler, carbonylcyanide m-chlorophenylhydrazone (CCCP), to fresh parathyroid tissues, 84.96% and 73.86% of sestamibi was released from the mitochondrial and tissue fragment components, respectively. In addition, sestamibi activity in the mitochondrial component of cryopreserved human parathyroid tissue decreased to the same amount as the CCCP-treated group. CONCLUSIONS: These data confirm that mitochondrial activity is the major component of sestamibi uptake by human parathyroid tissue in patients with hyperparathyroidism.
Asunto(s)
Mitocondrias/metabolismo , Glándulas Paratiroides/metabolismo , Tecnecio Tc 99m Sestamibi/farmacocinética , Criopreservación , Humanos , Hiperparatiroidismo/metabolismo , Microscopía Electrónica , Glándulas Paratiroides/ultraestructuraRESUMEN
The cellular response to a wide variety of stresses results in the synthesis of a family of stress response proteins termed heat shock proteins. Recent studies have demonstrated that heat shock proteins produced in response to an initial stress seem to protect against subsequent unrelated stresses. Importantly, hyperthermia-induced heat shock proteins provided protection from ischemia/reperfusion injury in several organ transplantation models. We hypothesized that free musculocutaneous flap survival could be improved by enhancing the flap's tolerance to relative ischemia by the prior induction of heat shock proteins. Accordingly, we determined the heat shock protein response in skin and muscle after systemic or local heating and examined the effect on free musculocutaneous flap survival in a rat model. Free musculocutaneous flaps incorporating thigh adductor muscles and a 2 x 6-cm2 skin paddle were transplanted to the ipsilateral groin in three groups of male Wistar rats. Systemically heated rats (n = 6) were anesthetized and incubated for 30 minutes at 42 degrees C 6 hours before free musculocutaneous tissue transfer. Locally heated rats (n = 6) were anesthetized, and their donor site anterior thigh was placed for 30 minutes on a heating block set at 44 degrees C 6 hours before free tissue transfer. Control rats (n = 5) did not have heating pretreatment but underwent identical anesthesia. Animals were sacrificed on postoperative day 3, at which time skin loss (cm2) and muscle viability, quantified by nitroblue tetrazolium staining time, were assessed in a blinded fashion. The skin and muscle from the free flap were analyzed for HSP72 mRNA and protein using quantitative Northern and Western blot techniques. All free musculocutaneous flaps were viable. However, the locally and systemically heated rats demonstrated a marked improvement of skin survival, which correlated with increased skin levels of HSP72. There were no differences in nitroblue tetrazolium muscle staining times or muscle levels of HSP72 among the three groups. These findings suggest that prior heat-induced heat shock proteins result in improvement in musculocutaneous flap survival, which may have direct clinical applications, especially in high-risk patients.
Asunto(s)
Proteínas de Choque Térmico/biosíntesis , Músculo Esquelético/trasplante , Trasplante de Piel/métodos , Colgajos Quirúrgicos/fisiología , Animales , Northern Blotting , Western Blotting , Temperatura Corporal , Modelos Animales de Enfermedad , Fiebre/metabolismo , Supervivencia de Injerto , Proteínas de Choque Térmico/análisis , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/fisiología , Calor/efectos adversos , Hipertermia Inducida , Hibridación in Situ , Indicadores y Reactivos , Isquemia/prevención & control , Masculino , Proteínas Musculares/análisis , Músculo Esquelético/metabolismo , Nitroazul de Tetrazolio , ARN Mensajero/análisis , Ratas , Ratas Wistar , Daño por Reperfusión/prevención & control , Factores de Riesgo , Método Simple Ciego , Piel/metabolismo , Temperatura Cutánea , Trasplante de Piel/fisiología , Estrés Fisiológico/metabolismo , MusloRESUMEN
We examined the level of heat shock proteins (HSPs) present in umbilical cord vessels and placenta following delivery. A 10 cm segment of fresh umbilical cord was obtained immediately after delivery and a placental biopsy was obtained following the third stage of labor. Apgar scores, cord pHs, and pertinent histories were recorded. Tissues were prepared and analyzed for HSP mRNA by in situ hybridization, Western blot, and immunohistochemistry. Sixteen patients were studied. HSP72 mRNA was selectively expressed in the vascular smooth muscle in all cord arteries and veins. This correlated with marked expression of HSP72 protein in the vascular media. Both HSP72/73 mRNA and protein expression were noted diffusely in the placenta. HSP27 protein was highly expressed in umbilical cords with lower levels present in placentas. There were no correlations between method of delivery, gestational age, Apgar score, cord pH, or fetal outcome to the magnitude or distribution of the HSP response. This is the first demonstration of HSP expression in the human maternal-fetal circulation.
Asunto(s)
Proteínas de Choque Térmico/análisis , Placenta/metabolismo , Cordón Umbilical/metabolismo , Western Blotting , Femenino , Humanos , Inmunohistoquímica , Embarazo , ARN Mensajero/análisisRESUMEN
The cellular response to a wide variety of stresses results in the synthesis of a family of proteins termed heat shock proteins (HSPs). To determine if acute allograft rejection could induce these proteins in a transplanted graft, we examined the HSP response to acute cardiac allograft rejection and analyzed the effect of immunosuppression upon this response. Donor hearts obtained from either Lewis (LEW) or ACI rats were heterotopically transplanted in recipient LEW rats. There were 4 experimental groups: untreated isografted (LEW to LEW) animals (n = 14), untreated allografted (ACI to LEW) animals (n = 14), cyclosporine-treated (10 mg/kg SQ/day) isografted animals (n = 12), and cyclosporine-treated allografted animals (n = 12). Animals were sacrificed on posttransplantation day 2, 4, or 6 (time of rejection for untreated allografts); n = 4-5 for each time point per group. At these times tissue obtained from the transplanted heart was examined histologically and analyzed for HSP72 by quantitative Northern and Western blots. The level of HSP72 in the untreated allografts progressively increased between 2, 4, and 6 days posttransplantation and was significantly greater than that of the untreated isografts at all time points. The HSP72 response in cyclosporine-treated allografts was significantly reduced at 4 and 6 days posttransplantation compared with the untreated allografts. In contrast, there was no difference in the HSP response in treated versus untreated isografts. Additionally, there was no difference in HSP levels in cyclosporine-treated isografts and allografts. These findings demonstrate that HSP expression in the transplanted heart correlates directly with the evolution of acute allograft rejection, and that immunosuppressive therapy inhibits the HSP response. These studies also raise the possibility of a functional role for HSPs in the allogeneic immune response.
Asunto(s)
Ciclosporina/administración & dosificación , Rechazo de Injerto/metabolismo , Trasplante de Corazón , Proteínas de Choque Térmico/biosíntesis , Inmunosupresores/administración & dosificación , Animales , Rechazo de Injerto/prevención & control , Masculino , Ratas , Ratas Endogámicas ACI , Ratas Endogámicas Lew , Trasplante HomólogoRESUMEN
In previous studies we demonstrated that restraint, or immobilization stress, resulted in the induction of HSP70 mRNA in the vasculature of rats. This response was confined to the vascular smooth muscle, was associated with alpha 1-adrenergic receptor stimulation, and was attenuated with age. In the present study, we examined the influence of transplanting aged vessels into young animals and young vessels into aged animals to determine if this age-related diminution was due to changes intrinsic to the aorta or the result of alterations in the environment in which the aorta resides. We demonstrate that restraint-induced HSP70 expression in transplanted vessels is influenced to a greater degree by the age of the recipient than by the age of the transplanted vessel. Transplantation of aged vessels into young animals leads to preservation of the response, whereas transplantation of young vessels into aged animals leads to attenuation. These findings indicate that the environment in which the vessel resides is a major factor in determining this molecular response to stress.
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Envejecimiento/metabolismo , Aorta Abdominal/trasplante , Aorta Torácica/trasplante , Proteínas HSP70 de Choque Térmico/metabolismo , Estrés Fisiológico/metabolismo , Animales , Aorta Abdominal/metabolismo , Aorta Torácica/metabolismo , Northern Blotting , Masculino , Modelos Biológicos , Ratas , Ratas Endogámicas F344 , Restricción FísicaRESUMEN
BACKGROUND: Glucocorticoids impair wound healing and cause surgical morbidity. Heat shock proteins are essential to cellular stress tolerance and are associated with glucocorticoids. The adrenal heat shock protein response is under hypothalmic-pituitary-adrenal-axis control, whereas the vascular response is associated with alpha-1 receptors. Because heat shock proteins affect cellular stress responses and are under hypothalmic-pituitary-adrenal-axis control in other tissues, we postulated an association between heat shock proteins and glucocorticoids in healing wounds. METHODS: Modified Hunt-Schilling wound chambers were implanted subcutaneously in rats. They received subcutaneous time-release dexamethasone (25 mg) or placebo pellets. Wound chamber heat shock protein 25 and heat shock protein 72/73 were serially assayed for 21 days with western analysis and immunocytochemistry. RESULTS: Dexamethasone caused Cushing's syndrome with approximately 10% weekly weight-loss and adrenal atrophy. Total wound tissue decreased 90% with profound differences in molecular wound responses manifested by decreased heat shock protein 25, 72, and 73 in animals treated with dexamethasone despite equal protein loads. Furthermore, dexamethasone caused heat shock protein 72 redistribution by immunocytochemistry. CONCLUSIONS: This study represents the first description of heat shock proteins in a wound healing model and demonstrates tissue-specific decrease of heat shock proteins with glucocorticoid therapy. Thus the heat shock protein response is intimately associated with normal wound healing and is profoundly altered in subjects with Cushing's syndrome. Manipulation of this response may have clinical importance in wound healing.
Asunto(s)
Síndrome de Cushing/fisiopatología , Proteínas de Choque Térmico/fisiología , Cicatrización de Heridas , Glándulas Suprarrenales/patología , Animales , Proteínas de Choque Térmico/análisis , Inmunohistoquímica , Masculino , Tamaño de los Órganos , Ratas , Ratas WistarRESUMEN
BACKGROUND: Surgical stress results in catecholamine secretion and selective induction of the major heat shock protein (HSP70) in the adrenal gland and in the vasculature. The adrenal response is cortical-specific and corticotropin-dependent. The vascular response occurs in the smooth muscle and is corticotropin-independent. We previously suggested that the vascular response was associated with adrenergic receptor stimulation. Herein, we report a series of experiments designed to test the hypothesis that aortic HSP70 messenger RNA (mRNA) induction occurs as a direct and specific response to alpha 1-adrenergic receptor stimulation. METHODS: Acute and chronic indwelling central venous catheter models were developed in the Wistar rat through which the following agents were infused: the alpha 1 agonist phenylephrine (0.14 mg/kg), the beta agonist isoproterenol (0.8 mg/kg), the alpha 1 antagonist prazosin (1 mg/kg), prazosin followed by phenylephrine, or saline solution alone. Hemodynamic responses were monitored; catecholamines were measured by high-performance liquid chromatography; 60 minutes after infusion, the animals were killed, and the adrenal glands and aortas were assayed for HSP70 mRNA expression on Northern blots. RESULTS: Alpha 1 stimulation with phenylephrine resulted in marked hypertension, a reflexive bradycardia, and marked induction of aortic HSP70 mRNA. This effect could be completely abolished when the alpha 1 antagonist prazosin was administered before phenylephrine treatment. The beta agonist isoproterenol failed to induce aortic HSP70. A significant catecholamine response only occurred after prazosin administration. CONCLUSIONS: These studies show a functional interaction between alpha 1 receptor stimulation and vascular HSP mRNA induction.
Asunto(s)
Glándulas Suprarrenales/metabolismo , Aorta/metabolismo , Proteínas de Choque Térmico/genética , ARN Mensajero/análisis , Receptores Adrenérgicos alfa 1/fisiología , Animales , Epinefrina/metabolismo , Masculino , Norepinefrina/metabolismo , Ratas , Ratas WistarRESUMEN
BACKGROUND: Stress adaptation requires interactions between the hypothalamic-pituitary-adrenal axis, the sympathetic nervous system, and a family of intracellular stress response proteins termed heat shock proteins (HSPs). These HSPs are present in every living organism and are selectively induced in the adrenal cortex and vascular smooth muscle after either surgical or restraint stress. METHODS: We perturbed the hypothalamic-pituitary-adrenal axis by implanting in the rat subcutaneous pellets containing either placebo or dexamethasone (25 mg), ovine corticotropin releasing factor (CRF, 0.5 mg), or the glucocorticoid antagonist RU 486 (5 mg) for 2 weeks before randomization to either 90 minutes of restraint stress or immediate sacrifice. The adrenal glands were weighed, trunk blood was collected for adrenocorticotropic hormone (ACTH) and corticosterone measurements, and RNA isolated from the adrenal glands and aorta was assayed for HSP70 messenger RNA expression by Northern analysis. RESULTS: Dexamethasone resulted in a twofold decrease in adrenal weight (p < 0.05). ACTH and corticosterone levels were markedly reduced in the dexamethasone treated group in the absence or presence of restraint stress. Restraint resulted in greater than 20-fold induction of HSP70 in both the adrenal gland and aorta of the placebo group compared with nonstressed controls (p < 0.01). Long-term dexamethasone treatment reduced adrenal HSP70 expression fourfold after restraint (p < 0.5), whereas neither CRF nor RU486 treatment significantly influenced the adrenal HSP70 response. Glucocorticoid manipulation with either dexamethasone or CRF did not significantly affect restraint-induced aortic HSP70 expression, whereas RU486 treatment resulted in a 50% diminution (p < 0.5) compared with placebo-treated controls. CONCLUSIONS: These data show dramatic induction of HSP70 messenger RNA expression in adrenal and aortic tissues after restraint stress. Differential organ specific HSP regulation is evidenced by the ability of the glucocorticoid dexamethasone to attenuate the adrenal but not the aortic response. The significant effect of RU486 on the aortic response suggests the possibility of vascular glucocorticoid-catecholamine interactions.
Asunto(s)
Proteínas de Choque Térmico/biosíntesis , Sistema Hipotálamo-Hipofisario/fisiología , Sistema Hipófiso-Suprarrenal/fisiología , Estrés Fisiológico/metabolismo , Glándulas Suprarrenales/metabolismo , Hormona Adrenocorticotrópica/sangre , Animales , Aorta/metabolismo , Catecolaminas/fisiología , Corticosterona/sangre , Dexametasona/farmacología , Proteínas de Choque Térmico/genética , Masculino , Mifepristona/farmacología , ARN Mensajero/análisis , Ratas , Ratas WistarRESUMEN
Adaptation to stress requires coordinated interactions between the vascular and endocrine systems. Previously we demonstrated that restraint stress induces the expression of the major heat shock protein, HSP70, in the adrenal cortex of the rat. Here we demonstrate that restraint also induces expression of HSP70 in the vasculature. We further demonstrate that the adrenal and vascular responses are differentially regulated: the adrenal response is adrenocorticotropin dependent, whereas the vascular response is under adrenergic control. In addition, the adrenal response is restricted to members of the HSP70 gene family, whereas in vascular tissue the low molecular weight HSP, HSP27, is also induced by restraint. Further characterization of the vascular response revealed that HSP70 induction occurred in both the thoracic and abdominal aortas as well as in the vena cava. However, no HSP70 induction was apparent in the heart or in a wide variety of other tissues examined. In situ hybridization showed that the vascular expression was localized to the aortic smooth muscle cells with minimal expression in the endothelium. Induction of HSP70 mRNA in both the adrenal cortex and aorta was followed by an elevation in HSP70 protein. Maximum HSP70 protein levels were seen within 3-12 h after restraint, but declined thereafter. Stress induced HSP70 expression was dramatically reduced with age, which may explain, in part, the diminished tolerance to stress seen in elderly individuals.
Asunto(s)
Corteza Suprarrenal/fisiología , Envejecimiento/metabolismo , Vasos Sanguíneos/metabolismo , Proteínas de Choque Térmico/biosíntesis , Sistema Hipotálamo-Hipofisario/fisiología , Sistema Hipófiso-Suprarrenal/fisiología , Estrés Fisiológico/metabolismo , Sistema Nervioso Simpático/fisiología , Agonistas alfa-Adrenérgicos/farmacología , Animales , Secuencia de Bases , Proteínas de Choque Térmico/genética , Masculino , Datos de Secuencia Molecular , Ratas , Ratas Endogámicas F344 , Ratas Wistar , Restricción FísicaRESUMEN
Previous studies indicate that a 20-30% decline in muscarinic acetylcholine receptor binding occurs in localized areas of rat brain during aging. In this study, reduced [3H]-quinuclidinyl benzilate binding was observed in striata from 24-25-month-old rats relative to 5-6-month-old animals using homogenate binding assays. To determine if the decline in receptor concentration occurs as a result of decreased receptor synthesis, the expression of the m1, m3, and m4 muscarinic receptor mRNAs as well as [3H]-QNB binding were determined in adjacent sections of young and old male rats using in situ hybridization and in vitro receptor autoradiography respectively. A significant decline in collective muscarinic receptor binding as assessed by [3H]-QNB was observed in the caudate putamen, olfactory tubercle, nucleus accumbens, and several frontal and parietal cortical areas. The only difference observed in muscarinic mRNA expression for any of the three subtypes examined was a decline in m1 hybridization in the olfactory tubercle. The results of this study demonstrate that the regional brain areas displaying age-related decreases in receptor binding do not correlate with those areas showing a decrease in muscarinic receptor expression. Apparently, the decline in muscarinic acetylcholine receptor density with age does not result from a decline in receptor gene expression.
Asunto(s)
Envejecimiento/metabolismo , Encéfalo/crecimiento & desarrollo , ARN Mensajero/metabolismo , Receptores Colinérgicos/genética , Animales , Autorradiografía , Secuencia de Bases , Encéfalo/metabolismo , Expresión Génica , Cinética , Ligandos , Masculino , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Quinuclidinil Bencilato , Ratas , Ratas EndogámicasRESUMEN
Administration of 17 beta-estradiol to mature (6-12 months) rats results in a more than 50% reduction in pituitary dopamine receptor concentrations, without affecting binding affinity. In contrast, when the same manipulation is performed on senescent (24-25 months) rats, negligible change in receptor concentration occurs. These results suggest that age-related increases in estrogen-stimulated prolactin release are not due to decreased dopaminergic inhibition at the receptor level.