RESUMEN
Apoptosis of blood neutrophils from healthy donors was studied under conditions of cell culturing with different concentrations of H(2)O(2), selective NO synthase inhibitor, and inductor of NO synthesis (L-arginine). In vitro incubation of neutrophilic leukocytes with 5 mM H(2)O(2) led to activation of the apoptotic program in neutrophils, which was seen from increased content of Bax protein in the cells and increased number of apoptotic cells in the culture. Increased content of annexin-positive cells after incubation of neutrophil culture with NO synthase inhibitor suggests involvement of NO in the regulation of neutrophil apoptosis under conditions of oxidative stress, while L-arginine prevented H(2)O(2)-induced programmed cell death.
Asunto(s)
Apoptosis , Neutrófilos/metabolismo , Óxido Nítrico/fisiología , Adolescente , Adulto , Anexinas/metabolismo , Apoptosis/efectos de los fármacos , Arginina/farmacología , Western Blotting , Células Cultivadas , Inhibidores Enzimáticos/farmacología , Femenino , Humanos , Peróxido de Hidrógeno/farmacología , Masculino , NG-Nitroarginina Metil Éster/farmacología , Neutrófilos/efectos de los fármacos , Óxido Nítrico/metabolismo , Óxido Nítrico Sintasa/antagonistas & inhibidores , Oxidantes/farmacología , Estrés Oxidativo/fisiología , Especies Reactivas de Oxígeno/metabolismo , Adulto Joven , Proteína X Asociada a bcl-2/metabolismoRESUMEN
Programmed death of peripheral blood mononuclear cells from healthy donors was studied during culturing with various concentrations of H(2)O(2) and selective inhibitors of JNK (SP600125) and p38 MAPK (ML3403). In vitro incubation of mononuclear leukocytes with 1 mM H(2)O(2) stimulated apoptotic cell death. Treatment with inhibitors (SP600125 and ML3403) during in vitro oxidative stress prevented the increase in the number of annexin-positive mononuclear cells. Our results indicate that MAP kinases JNK and p38 are involved in the mechanisms of oxidative dysregulation of apoptosis.
