RESUMEN
Biochemical parameters of transformation of fresh subcutaneous fat into adipocere have been studied in experiment. Activation of lipid peroxidation is a significant component of the mechanism of adipocere transformation, which is proven by a drastic increase in the level of Schiff's bases in adipocere in comparison with subcutaneous fat. The concentrations of myristic, palmitic, and stearic fatty acids increase during the formation of adipocere, while the content of linoleic acid decreases in comparison with the unchanged human subcutaneous fat.
Asunto(s)
Tejido Adiposo/metabolismo , Ácidos Grasos/metabolismo , Peroxidación de Lípido , Cambios Post Mortem , Tejido Adiposo/química , Animales , Medicina Legal , Humanos , Ácidos Linoleicos/metabolismo , Ácidos Mirísticos/metabolismo , Ácidos Palmíticos/metabolismo , Ácidos Esteáricos/metabolismoRESUMEN
Scanty and contradictory published data made the authors carry out examinations of human tissues in a state of adipocere using visual, stereomicroscopic, biochemical methods, and numerous physical tests. Adipocere developing under natural conditions and extracted from human corpses and experimentally obtained adipocere were found to be quite the same. Biochemical studies of adipocere to detect phospholipids and the percent of ratio of total lipids and their classes were carried out for the first time.
Asunto(s)
Metabolismo de los Lípidos , Cambios Post Mortem , Cromatografía en Capa Delgada/métodos , Humanos , Lípidos/análisis , MicroscopíaRESUMEN
Wistar rats were injected with 2-14C-propionate in a dose of 30 mu Ci/100 g bw, 2 h after food intake. Two hours after isotope injection the rats were decapitated to determine specific radioactivity (SR) in liver and brain lipids, in liver phosphatidylcholine (PC) and its structural components. The label was incorporated in liver lipids in a far greater amount. In liver PC, SR appeared the highest in glycerin and less higher in the fraction of higher fatty acids. The least amount of the label from 2-14C-propionate was incorporated in choline. The fact of the label incorporation in choline was recorded for the first time.
Asunto(s)
Colina/biosíntesis , Digestión , Propionatos/metabolismo , Animales , Encéfalo/metabolismo , Metabolismo de los Lípidos , Hígado/metabolismo , Masculino , Fosfatidilcolinas/metabolismo , Ratas , Ratas EndogámicasRESUMEN
Incubation of tissue of homogenates of peripheral nerves, liver, and subcutaneous adipose tissue of rabbits with [1-14C]palmitic acid and intraperitoneal injection of this preparation into rats, in a dose of 20 muCi per 100 g of weight of the animals, permitted establishment of the presence of enzyme systems of biosynthesis of n-alkanes in the tissues of higher animals. Evidence of the biosynthesis of n-alkanes in the animal organism represents a new aspect of our knowledge of the metabolism of this class of compounds in animal tissues.
Asunto(s)
Hidrocarburos/metabolismo , Ácidos Palmíticos/metabolismo , Tejido Adiposo/metabolismo , Animales , Hígado/metabolismo , Masculino , Especificidad de Órganos , Nervios Periféricos/metabolismo , Ratas , InaniciónRESUMEN
Ability of epithelium of rat small intestine mucose to synthetize arachidonic acid was studied in vivo and in vitro. Linoleic acid and 2-14C-acetate were used as precursors. The fatty acids were fractionated using thin-layer chromatography on silica gel impregnated with AgNO3. The label was incorporated into all the fractions but the highest radioactivity was found in the fraction of arachidonic acid; this suggests that the enzyme systems, carrying out the synthesis of polyenic fatty acids, occur in the mucosa of small intestine.