RESUMEN
A precise, sensitive, accurate, and validated reverse-phase high-performance liquid chromatography (RP-HPLC) method with a bioanalytical approach was utilized to analyze Cabazitaxel (CBZ) in rat plasma. Comparative research on extraction recoveries was performed between traditional liquid-liquid extraction (LLE) and synthesized graphene oxide (GO) based magnetic solid phase extraction (GO@MSPE). The superparamagnetic hybrid nanosorbent was synthesized using the combination of iron oxide and GO and subsequently applied for extraction and bioanalytical quantification of CBZ from plasma by (HPLC-PDA) analysis. Fourier- transform infrared spectroscopy (FT-IR), particle size, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis were employed in the characterization of synthesized GO@MSPE nanosorbent. The investigation was accomplished using a shim pack C18 column (150â¯mm×4.6â¯mm, 5⯵m) with a binary gradient mobile phase consisting of formic acid: acetonitrile: water (0.1:75:25, v/v/v) at a 0.8â¯mL/min flow rate, and a λmax of 229â¯nm. The limits of detection (LOD) and quantitation (LOQ) have been determined to be 50 and 100â¯ng/mL for both LLE and SPE techniques. The linearity range of the approach encompassed from 100 to 5000â¯ng/mL and was found to be linear (coefficient of determination > 0.99) for CBZ. The proposed method showed extraction recovery of 76.8-88.4% for the synthesized GO@MSPE and 69.3-77.4% for LLE, suggesting that the proposed bioanalytical approach was robust and qualified for all validation parameters within the acceptable criteria. Furthermore, the developed hybrid GO@MSPE nanosorbent with the help of the proposed RP-HPLC method, showed a significant potential for the extraction of CBZ in bioanalysis.
Asunto(s)
Grafito , Límite de Detección , Extracción Líquido-Líquido , Extracción en Fase Sólida , Animales , Cromatografía Líquida de Alta Presión/métodos , Ratas , Extracción Líquido-Líquido/métodos , Grafito/química , Extracción en Fase Sólida/métodos , Taxoides/sangre , Taxoides/química , Masculino , Reproducibilidad de los Resultados , Espectroscopía Infrarroja por Transformada de Fourier/métodosRESUMEN
An intimate physical mixture of graphene oxide (GO) and semiconducting organic molecules like bromophenathrene (BrPh) and bromopyrene (BrPy) was prepared by using a ball milling technique. The structural, microstructural, physical and chemical properties of the mixtures (20 wt% of GO) were analyzed by X-ray diffraction, SEM, FT-IR, TGA and TCSPC studies. Furthermore, the electrochemical properties like AC electrical conductivity, transient photocurrent response (PCTR) and open circuit voltage (OCVD) of the samples were analyzed. It has been observed from TCSPC and OCVD measurements that 20 wt% of GO in the semiconductor composite leads to an enhanced life-time of photo-generated charge carriers. The physical mixture composites exhibit a higher photocurrent than pure BrPh and BrPy.