RESUMEN
Within all cattle production systems, veal calves are the most severely affected by abomasal damage, with current prevalence at slaughter ranging from 70 to 93% of all animals affected. Although most damage is found in the pyloric region of the abomasum, fundic lesions are also found. Despite past research into the etiology of abomasal damage and the many risk factors that have been proposed, consensus on the causal factors of abomasal damage in veal calves has not yet been reached. The aim of this review was to integrate and analyze available information on the etiology of, and possible risk factors for, abomasal damage in veal calves. We describe various proposed pathways through which risk factors may contribute to damage formation and conclude that the etiology of abomasal damage is most likely multifactorial, with diet being a main contributor. Pyloric lesions, the most common type of damage in veal calves, are likely the result of large and infrequent milk and solid feed meals, whereas fundic lesions may be caused by stress, although the evidence for this is inconclusive. Providing calves with multiple smaller milk and solid feed meals (or ad libitum provision) may decrease abomasal damage. In future research, ulcers, erosions, and scars as well as fundic and pyloric lesions should be recorded separately, because etiologies of these may differ. Further research is required to understand the exact pathway(s) by which milk replacer causes abomasal damage in veal calves; that is, whether low abomasal pH, overloading, or composition are important. Further research is also required to elucidate whether rapid intake of milk replacer and solid feed, which is influenced by restricted amounts fed, inter-calf competition, and calf breed, increases abomasal damage. Research is also needed into the effect of medication and nutrient deficiencies other than iron. The types of experimental designs that can be used for future research could be enhanced if a means to assess abomasal damage antemortem is developed. We conclude that it is unlikely that abomasal or ruminal hairballs, iron deficiency, water provision, and various infections and diseases are significant contributors to abomasal damage in veal calves.
Asunto(s)
Abomaso , Enfermedades de los Bovinos/etiología , Gastropatías/veterinaria , Alimentación Animal/efectos adversos , Animales , Bovinos , Dieta/efectos adversos , Dieta/veterinaria , Leche , Factores de Riesgo , Gastropatías/etiologíaRESUMEN
To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT-PCR). After SBV infection both animals in the study showed viraemia (qRT-PCR) with fever and diarrhoea. SBV RNA could be detected in semen from both animals. The highest SBV RNA concentrations in semen were found in the first week (days 4-7 post-inoculation) but concentrations were relatively low (Ct values 30-39). Viable SBV was only isolated from blood samples and not from semen or genital tissues.
Asunto(s)
Infecciones por Bunyaviridae/veterinaria , Enfermedades de los Bovinos/virología , Orthobunyavirus/aislamiento & purificación , Semen/virología , Animales , Infecciones por Bunyaviridae/virología , Bovinos , Chlorocebus aethiops , Enfermedades Transmisibles Emergentes/virología , Genitales Masculinos/virología , Ganglios Linfáticos/virología , Masculino , ARN Viral/análisis , Células VeroRESUMEN
Rift Valley fever virus (RVFV) is a zoonotic bunyavirus that causes abortions in domesticated ruminants. Sheep breeds exotic to endemic areas are reportedly the most susceptible to RVFV infection. Within the scope of a risk assessment program of The Netherlands, we investigated the susceptibility of a native breed of gestating sheep to RVFV infection. Ewes were infected experimentally during the first, second, or third trimester of gestation. Mortality was high among ewes that developed viremia. Four of 11 inoculated ewes, however, did not develop detectable viremia nor clinical signs and did not seroconvert for immunoglobulin G (IgG) or IgM antibodies. Surprisingly, these ewes were found to contain viral RNA in maternal and fetal organs, and the presence of live virus in fetal organs was demonstrated by virus isolation. We demonstrate that RVFV can be transmitted vertically in the absence of detectable maternal viremia.
Asunto(s)
Transmisión Vertical de Enfermedad Infecciosa/veterinaria , Complicaciones Infecciosas del Embarazo/veterinaria , Fiebre del Valle del Rift/transmisión , Virus de la Fiebre del Valle del Rift/fisiología , Enfermedades de las Ovejas/transmisión , Animales , Anticuerpos Antivirales/sangre , Femenino , Países Bajos/epidemiología , Embarazo , Complicaciones Infecciosas del Embarazo/mortalidad , Complicaciones Infecciosas del Embarazo/virología , ARN Viral/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria , Fiebre del Valle del Rift/mortalidad , Fiebre del Valle del Rift/virología , Virus de la Fiebre del Valle del Rift/aislamiento & purificación , Ovinos , Enfermedades de las Ovejas/mortalidad , Enfermedades de las Ovejas/virología , Viremia/veterinariaRESUMEN
The study aimed to assess the in vivo and postmortem prevalence of respiratory disorders in veal calves and investigate risk factors associated with them. A cross-sectional study was carried out in 174 farms in the 3 major veal meat-producing countries in Europe (50 in France, 100 in the Netherlands, and 24 in Italy). Trained veterinarians visually evaluated individual calves of 1 batch per farm at 3 and 13 wk after arrival and at 2 wk before slaughter to assess the prevalence of hampered respiration, nasal discharge, and coughing. A random sample of lungs belonging to calves of the same batch was monitored at the slaughterhouse for mild to moderate or severe signs of pneumonia, and presence of pleuritis. Data regarding veal calf housing, feeding, and management and specific characteristics of the batch were collected through an interview with the stockperson, and the potential of these as respiratory disease risk factors was assessed. Regardless of the stage of fattening, the prevalence of in vivo signs of respiratory disorders in calves was always <7%. This low prevalence was likely the outcome of the general implementation by veal producers of standardized practices such as prophylaxis, all-in/all-out, and individual daily checks of the calves, which are recognized tools for effective disease prevention and management. However, at postmortem inspection, 13.9% and 7.7% of lungs showed mild to moderate and severe signs of pneumonia, respectively, and 21.4% of the inspected lungs had pleuritis. Thus, even mild clinical signs of respiratory disorder in calves at specific time points during the fattening period may be associated with high prevalence of lungs with lesions at slaughter. Alternatively, clinical symptoms recorded during routine visual inspections of veal calves on-farm may be poor predictors of the true prevalence of respiratory disease in calves. Among all potential risk factors considered, those concerning the characteristics of the batch were predominant but factors related to housing, management and feeding equipment were also relevant. Different risk factors were involved at different stages of the fattening period. Therefore, to overcome respiratory disorders in veal calves, different solutions may apply to different stages of the fattening period.
Asunto(s)
Enfermedades de los Bovinos/epidemiología , Enfermedades Respiratorias/veterinaria , Animales , Animales Recién Nacidos , Bovinos , Estudios Transversales , Industria Lechera/métodos , Industria Lechera/normas , Femenino , Francia/epidemiología , Italia/epidemiología , Masculino , Países Bajos/epidemiología , Prevalencia , Enfermedades Respiratorias/epidemiología , Enfermedades Respiratorias/etiología , Factores de RiesgoRESUMEN
In April 2009 a new influenza A/H1N1 strain, currently named "pandemic (H1N1) influenza 2009" (H1N1v), started the first official pandemic in humans since 1968. Several incursions of this virus in pig herds have also been reported from all over the world. Vaccination of pigs may be an option to reduce exposure of human contacts with infected pigs, thereby preventing cross-species transfer, but also to protect pigs themselves, should this virus cause damage in the pig population. Three swine influenza vaccines, two of them commercially available and one experimental, were therefore tested and compared for their efficacy against an H1N1v challenge. One of the commercial vaccines is based on an American classical H1N1 influenza strain, the other is based on a European avian H1N1 influenza strain. The experimental vaccine is based on reassortant virus NYMC X179A (containing the hemagglutinin (HA) and neuraminidase (NA) genes of A/California/7/2009 (H1N1v) and the internal genes of A/Puerto Rico/8/34 (H1N1)). Excretion of infectious virus was reduced by 0.5-3 log(10) by the commercial vaccines, depending on vaccine and sample type. Both vaccines were able to reduce virus replication especially in the lower respiratory tract, with less pathological lesions in vaccinated and subsequently challenged pigs than in unvaccinated controls. In pigs vaccinated with the experimental vaccine, excretion levels of infectious virus in nasal and oropharyngeal swabs, were at or below 1 log(10)TCID(50) per swab and lasted for only 1 or 2 days. An inactivated vaccine containing the HA and NA of an H1N1v is able to protect pigs from an infection with H1N1v, whereas swine influenza vaccines that are currently available are of limited efficaciousness. Whether vaccination of pigs against H1N1v will become opportune remains to be seen and will depend on future evolution of this strain in the pig population. Close monitoring of the pig population, focussing on presence and evolution of influenza strains on a cross-border level would therefore be advisable.
Asunto(s)
Subtipo H1N1 del Virus de la Influenza A/fisiología , Vacunas contra la Influenza/inmunología , Infecciones por Orthomyxoviridae/veterinaria , Enfermedades de los Porcinos/inmunología , Enfermedades de los Porcinos/prevención & control , Animales , Subtipo H1N1 del Virus de la Influenza A/aislamiento & purificación , Subtipo H3N2 del Virus de la Influenza A/genética , Subtipo H3N2 del Virus de la Influenza A/inmunología , Infecciones por Orthomyxoviridae/inmunología , Infecciones por Orthomyxoviridae/prevención & control , Virus Reordenados/inmunología , Sus scrofa , Porcinos , Enfermedades de los Porcinos/virología , Vacunas de Productos Inactivados/inmunologíaRESUMEN
In 2009 a new influenza A/H1N1 virus strain ("pandemic (H1N1) 2009", H1N1v) emerged that rapidly spread around the world. The virus is suspected to have originated in swine through reassortment and to have subsequently crossed the species-barrier towards humans. Several cases of reintroduction into pigs have since been reported, which could possibly create a reservoir for human exposure or ultimately become endemic in the pig population with similar clinical disease problems as current swine influenza strains. A soluble trimer of hemagglutinin (HA), derived from the H1N1v, was used as a vaccine in pigs to investigate the extent to which this vaccine would be able to protect pigs against infection with the H1N1v influenza strain, especially with respect to reducing virus replication and excretion. In a group of unvaccinated control pigs, no clinical symptoms were observed, but (histo)pathological changes consistent with an influenza infection were found on days 1 and 3 after inoculation. Live virus was isolated from the upper and lower respiratory tract, with titres up to 10(6) TCID(50) per gram of tissue. Furthermore, live virus was detected in brain samples. Control pigs were shedding live virus for up to 6 days after infection, with titres of up to 10(5) TCID(50) per nasal or oropharyngeal swab. The soluble H1N1v HA trimer diminished virus replication and excretion after a double vaccination and subsequent challenge. Live virus could not be detected in any of the samples taken from the vaccinated pigs. Vaccines based on soluble HA trimers provide an attractive alternative to the current inactivated vaccines.
Asunto(s)
Glicoproteínas Hemaglutininas del Virus de la Influenza/inmunología , Subtipo H1N1 del Virus de la Influenza A/inmunología , Vacunas contra la Influenza/inmunología , Infecciones por Orthomyxoviridae/prevención & control , Animales , Anticuerpos Antivirales/sangre , Pruebas de Inhibición de Hemaglutinación , Pruebas de Neutralización , Infecciones por Orthomyxoviridae/inmunología , Proteínas Recombinantes/inmunología , Porcinos , Vacunas Sintéticas/inmunología , Esparcimiento de VirusRESUMEN
The study aimed at assessing the prevalence of poor rumen development, presence of rumen plaques, rumen papillae hyperkeratinization, and abomasal lesions in veal calves and to investigate risk factors for their occurrence at the farm level. Within a wide cross-sectional study, a sample of 170 veal farms representative of the European veal meat production systems was considered in the 3 major producing countries (99 in the Netherlands, 47 in France, and 24 in Italy). An average of 59 ± 10 (SD) rumens and abomasa belonging to calves from a single batch per farm were inspected at the abattoir by trained observers to assess the incidence of these gastrointestinal disorders. Potential risk factors for their occurrence related to farm management, housing, and to the feeding plan were obtained by a questionnaire submitted to the stockperson. Prevalence of poor rumen development (almost no papillae present), rumen plaques, and hyperkeratinization were 60.4, 31.4, and 6.1% of rumens, respectively, whereas abomasal lesions in the pyloric area were recorded in 74.1% of abomasa. Independent variables related to the feeding system confirmed to be the main risk factors for the occurrence of gastrointestinal disorders in veal calves. However, additional risk sources for each given problem were identified among housing and management variables. The provision of a low amount of solid feed (≤ 50kg of dry matter/head per cycle) was a relevant risk for rumen underdevelopment. Rumen wall alterations (plaques and hyperkeratinization) and abomasal lesions were instead associated with the administration of large quantities of solids (151-300 kg of dry matter/head per cycle) in calves receiving milk replacer during the entire fattening cycle. Among the types of solid feed, cereal grain acted as a preventive measure for low rumen development, whereas it was a risk factor for the occurrence of rumen plaques, papillae hyperkeratinization, and abomasal lesions. Some housing and management options adopted to improve veal calf welfare (i.e., higher space allowance and use of heating) were associated with lower risk for gastrointestinal disorders.
Asunto(s)
Abomaso/patología , Enfermedades de los Bovinos/epidemiología , Queratinas/metabolismo , Rumen/patología , Gastropatías/veterinaria , Mataderos , Bienestar del Animal , Animales , Bovinos , Enfermedades de los Bovinos/patología , Estudios Transversales , Femenino , Francia/epidemiología , Italia/epidemiología , Masculino , Países Bajos/epidemiología , Prevalencia , Factores de Riesgo , Rumen/crecimiento & desarrollo , Gastropatías/epidemiología , Gastropatías/patologíaRESUMEN
Sixty-four male Holstein-Friesian x Dutch Friesian veal calves (46 +/- 3.0 kg) were used to evaluate the effect of the inclusion of different levels and sources of dietary roughage on animal performance and rumen development. Treatments consisted of 1) C100 = concentrate only; 2) C70-S30 = concentrate (70%) with straw (30%), 3) C70-G30 = concentrate (70%) with dried grass (30%), 4) C70-G15-S15 = concentrate (70%) with dried grass (15%) and straw (15%), 5) C70-CS30 = concentrate (70%) with corn silage (30%), 6) C40-CS60 = concentrate (40%) with corn silage (60%), 7) C70-CS30-AL = concentrate (70%) with corn silage (30%) ad libitum, 8) C70-G15-S15-AL = concentrate (70%) with dried grass (15%) and straw (15%) ad libitum. All dietary treatments were provided in addition to a commercial milk replacer. Concentrate was provided as pellets and roughage was chopped. The dietary treatments 1 to 6 were supplied restrictedly to a maximum of 750 g of dry matter (DM) per day, whereas treatments 7 and 8 were offered ad libitum in combination with a reduced amount of milk replacer. Calves were euthanized after 10 wk. Straw supplementation (C70-S30 vs. C70-G30 and C70-CS30) reduced DM intake, and ad libitum supply of concentrate and roughage increased DM intake. Roughage addition did not affect growth performance. Rumen fermentation was characterized by low pH and high total volatile fatty acids and reducing sugar concentrations. Calves fed ad libitum showed lower ruminal lactate concentrations than calves fed restrictedly. Ammonia concentrations were highest in calves fed C-100 and lowest in calves fed ad libitum. The recovery of CoEDTA (added to milk replacer) varied between 20.5 and 34.9%, indicating that significant amounts of milk entered the rumen. Roughage addition decreased the incidence of plaque formation (rumen mucosa containing focal or multifocal patches with coalescing and adhering papillae covered by a sticky mass of feed, hair and cell debris) and the incidence of calves with poorly developed rumen mucosa. However, morphometric parameters of the rumen wall were hardly influenced by the type and level of roughage. Ruminal polysaccharide-degrading enzyme activities reflected the adaptation of the microorganisms to the dietary concentrate and roughage source. Results indicated that in veal calves, the addition of roughage to concentrate diets did not affect growth performance and positively influenced the macroscopic appearance of the rumen wall.
Asunto(s)
Bovinos/crecimiento & desarrollo , Bovinos/metabolismo , Dieta/veterinaria , Fibras de la Dieta/metabolismo , Sustitutos de la Leche/metabolismo , Rumen/crecimiento & desarrollo , Rumen/metabolismo , Alimentación Animal/análisis , Animales , Ingestión de Alimentos , Ácidos Grasos Volátiles/análisis , Fermentación/fisiología , Contenido Digestivo/química , Contenido Digestivo/enzimología , Concentración de Iones de Hidrógeno , Ácido Láctico/análisis , Masculino , Rumen/anatomía & histología , Factores de Tiempo , Aumento de PesoRESUMEN
The objective of this experiment was to examine the effects of concentrates in feed, differing in carbohydrate source, on the rumen development of veal calves. For this purpose, 160 male Holstein Friesian x Dutch Friesian crossbred calves were used in a complete randomized block design with a 5 x 2 factorial arrangement. Dietary treatments consisted of 1) a milk replacer control, 2) a pectin-based concentrate, 3) a neutral detergent fiber-based concentrate, 4) a starch-based concentrate, and 5) a mixed concentrate (equal amounts of the concentrates in treatments 2, 3, and 4). Concentrate diets were provided as pellets in addition to a commercial milk replacer. Calves were euthanized at either 8 or 12 wk of age. Plasma acetate and beta-hydroxybutyrate (BHBA) were measured as indicators of rumen development. Empty rumen weight was determined, and wall samples were taken at slaughter. In most calves, a poorly developed rumen mucosa was observed. Coalescing rumen papillae with embedded hair, feed particles, and cell debris were found in all calves fed the concentrate diets. Calves fed concentrates had significantly heavier rumens than calves fed the control diet. In the dorsal location of the rumen, calves fed concentrate diets showed an increased ratio of mucosa to serosa length compared with calves fed the control diet, whereas in the ventral location only, calves fed the pectin and mixed diets showed larger ratios of mucosa to serosa length. Mucosa thickness and muscle thickness were greater in the ventral and dorsal locations of the rumen, respectively. In both locations, the NDF diet resulted numerically in the lowest mucosa thickness and highest muscle thickness among the concentrate treatments. At 8 wk, calves fed the concentrate diets had higher plasma acetate concentrations than calves on the control treatment. However, at 12 wk, only NDF-fed calves showed significantly higher plasma acetate concentrations. The plasma BHBA concentrations of calves at 8 wk of age fed the pectin and mixed diets were higher than those of the control diet-fed calves. At 12 wk, no differences in BHBA concentrations were observed among treatments. Results of a principal component analysis indicated that, in addition to rumen volatile fatty acid concentrations, other factors were likely to affect rumen development, and that the relationships between rumen development and individual types of volatile fatty acids present in the rumen liquor were similar. Also, variations in rumen development coincided with variations in plasma acetate and BHBA concentrations.
Asunto(s)
Bovinos/crecimiento & desarrollo , Dieta/veterinaria , Carbohidratos de la Dieta/administración & dosificación , Suplementos Dietéticos , Rumen/crecimiento & desarrollo , Ácido 3-Hidroxibutírico/sangre , Acetatos/sangre , Animales , Carbohidratos de la Dieta/metabolismo , Masculino , Modelos Estadísticos , Análisis de Componente Principal , Distribución Aleatoria , Factores de TiempoRESUMEN
This paper describes a methodology to quantify the transmission of Actinobacillus (A.) pleuropneumoniae from subclinically infected carrier pigs to susceptible contact pigs, and to test the effect of possible interventions on the transmission. The methodology includes the design of a transmission experiment, and a method with which A. pleuropneumoniae transmission can be quantified and with which the effect of an intervention on the transmission can be tested. The experimental design consists of two parts. First, subclinically infected carrier pigs are created by contact exposure of specific-pathogen-free pigs to endobronchially inoculated pigs. Second, transmission is observed from the group of carrier pigs to a second group of susceptible contact pigs after replacing the inoculated pigs by new contact pigs. The presented analytical method is a generalised linear model (GLM) with which the effect of an intervention on the susceptibility and infectivity can be tested separately, if the transmission is observed in heterogeneous populations. The concept of the experimental transmission model is illustrated by describing an A. pleuropneumoniae transmission experiment in which the effect of vaccination on the susceptibility is quantified. Although it could not be demonstrated that vaccination has an effect on the susceptibility of pigs, it was demonstrated that nasal excretion of A. pleuropneumoniae is related to the infectivity of pigs.
Asunto(s)
Infecciones por Actinobacillus/transmisión , Infecciones por Actinobacillus/veterinaria , Actinobacillus pleuropneumoniae/crecimiento & desarrollo , Portador Sano/microbiología , Pleuroneumonía Contagiosa/microbiología , Enfermedades de los Porcinos/microbiología , Enfermedades de los Porcinos/transmisión , Infecciones por Actinobacillus/microbiología , Infecciones por Actinobacillus/prevención & control , Animales , Anticuerpos Antivirales/sangre , Pulmón/microbiología , Modelos Inmunológicos , Pruebas de Neutralización/veterinaria , Tonsila Palatina/microbiología , Pleuroneumonía Contagiosa/prevención & control , Pleuroneumonía Contagiosa/transmisión , Distribución Aleatoria , Organismos Libres de Patógenos Específicos , Porcinos , Enfermedades de los Porcinos/prevención & control , Vacunación/veterinaria , Vacunas Virales/inmunología , Vacunas Virales/normasRESUMEN
Ten transmission trials with Actinobacillus pleuropneumoniae were carried out. The observed transmission was highly variable, which was surprising since the design of the trials was very similar. We investigated whether the variable transmission could be explained by variation in infectivity of A. pleuropneumoniae infected pigs. We looked for measurable characteristics, which could be indicative for infectious pigs or for the level of infectivity. The characteristic that appeared to be most indicative for a pig being infectious was an A. pleuropneumoniae positive tonsil at necropsy. The characteristic that was correlated to the level of infectivity was the number of A. pleuropneumoniae colonies isolated from the nasal swab, i.e. the probability for an infectious pig to infect a susceptible pig was tenfold higher on days where at least ten colonies were isolated. In this study it is shown that it is possible to measure the bacterial transmission of A. pleuropneumoniae under controlled circumstances if variation in infectivity is taken into account.
Asunto(s)
Infecciones por Actinobacillus/veterinaria , Actinobacillus pleuropneumoniae/patogenicidad , Enfermedades de los Porcinos/transmisión , Porcinos/microbiología , Infecciones por Actinobacillus/transmisión , Animales , Femenino , Masculino , Tonsila Palatina/microbiologíaRESUMEN
A 2 year-old cow with abnormal behaviour was observed during a farm visit. Rectal palpation of the cow revealed the presence of a mass of at least 12 cm in diameter. After further examination, it appeared that 'ovarian tumour' was the most likely differential diagnosis. In order to confirm this diagnosis, blood samples were drawn and analysed for plasma progesterone and plasma oestradiol-17 beta concentrations. Also, the gross pathology and histology of the mass were evaluated. The combination of the clinical presentation of the cow, the hormone concentrations, and the histological appearance of the mass confirmed the diagnosis ovarian tumour. The tumour was classified as granulosa-theca cell tumour.
Asunto(s)
Enfermedades de los Bovinos/diagnóstico , Tumor de Células de la Granulosa/veterinaria , Neoplasias Ováricas/veterinaria , Neoplasia Tecoma/veterinaria , Animales , Bovinos , Enfermedades de los Bovinos/sangre , Diagnóstico Diferencial , Estradiol/sangre , Femenino , Tumor de Células de la Granulosa/sangre , Tumor de Células de la Granulosa/diagnóstico , Neoplasias Ováricas/sangre , Neoplasias Ováricas/diagnóstico , Progesterona/sangre , Neoplasia Tecoma/sangre , Neoplasia Tecoma/diagnósticoRESUMEN
To study the effect of a previous porcine respiratory and reproductive syndrome-infection (PRRS) of the respiratory tract on influenza virus and Actinobacillus pleuropneumoniae (App) infections, 3-week-old specific-pathogen-free (spf) piglets were intranasally infected with PRRS virus. One week later, when the lung alveolar macrophages of PRRSV infected pigs were lowest in number, a second infection was applied by intranasal aerosol of influenza virus H3N2 or by endobronchial instillation of a mildly virulent App. The first experiment consisted of two groups (only influenza infection or dual PRRSV/influenza infection). A second experiment consisted of 4 groups (only influenza infection, only PRRSV infection, dual PRRSV/influenza infection and uninfected controls). At day 2, 4, 14 and 21 after influenza infection, two pigs were killed and sampled for virological and histopathological examination. Influenza H3N2 virus caused only a mild inflammation of the smaller bronchioli. Previous PRRSV infection did not influence clinical signs during influenza infection. Next, we studied in two experiments the effect of dual PRRSV/App infection during the acute stage at two days after App infection. In a third experiment, the influence of PRRSV on more chronic stages of App infection was studied at two weeks after the App infection. At the end of the experiments, the pigs were killed. Lungs were ranked according to size and kind of the lesions. Lesions were cut and measured, samples were taken for virological and histopathological examination. Statistical analysis of the ranked lung-lesions in the first experiment showed a distinct but small effect of previous PRRSV infection on the development of App-lesions. In PRRSV infected pigs. App produced a more severe disease. The second and third experiment however failed to show any influence of the previous PRRSV infection on the App infection. We conclude that previous PRRSV infection of the respiratory tract of spf pigs does not necessarily enhance the severity of secondary infections of the respiratory tract.
Asunto(s)
Infecciones por Actinobacillus/veterinaria , Infecciones por Orthomyxoviridae/veterinaria , Síndrome Respiratorio y de la Reproducción Porcina/fisiopatología , Enfermedades de los Porcinos , Infecciones por Actinobacillus/complicaciones , Infecciones por Actinobacillus/patología , Infecciones por Actinobacillus/fisiopatología , Actinobacillus pleuropneumoniae , Animales , Pulmón/patología , Pulmón/virología , Infecciones por Orthomyxoviridae/complicaciones , Infecciones por Orthomyxoviridae/patología , Infecciones por Orthomyxoviridae/fisiopatología , Proyectos Piloto , Síndrome Respiratorio y de la Reproducción Porcina/patología , PorcinosRESUMEN
In a flock of sheep to which food with excess copper (= Cu) had been fed for some months 39 animals died of chronic copper toxicosis. In 5 sheep of this flock the plasma concentrations of the enzymes glutamate dehydrogenase (GLDH), sorbitol dehydrogenase (SDH), aspartate-amino-transferase (ASAT) and copper were measured several times over a period of 100 days. At day 23, 58 and 97 needle biopsies of the liver were taken. The biopsies were used for histological examination and Cu-analysis. After 100 days the sheep were slaughtered and chronic copper poisoning was diagnosed by raised Cu concentration and typical histological alterations in the liver. The activities of GLDH and SDH in the plasma of the 5 sheep were consistently above normal, ASAT activity and the plasma Cu level were raised only occasionally. The results of Cu determination in biopsy samples were of little predictive value compared with post mortem Cu analysis of the liver.
Asunto(s)
Cobre/envenenamiento , Hígado/patología , Enfermedades de las Ovejas/inducido químicamente , Animales , Aspartato Aminotransferasas/sangre , Bilirrubina/sangre , Biopsia con Aguja/veterinaria , Cobre/análisis , Cobre/sangre , Creatina Quinasa/sangre , Femenino , Glutamato Deshidrogenasa/sangre , Hematócrito/veterinaria , Hemoglobinas/análisis , L-Iditol 2-Deshidrogenasa/sangre , Hígado/química , Hígado/efectos de los fármacos , Ovinos , Enfermedades de las Ovejas/sangre , Enfermedades de las Ovejas/patologíaRESUMEN
A pig grower ration containing olaquindox at a concentration of 778 mg/kg was accidentally fed to 10 respectively 16 weeks old weaner pigs. The subsequent intoxication was characterized by poor growth and long-term disturbances of renal function and electrolyte metabolism. A deficiency of aldosterone, produced by a selective degeneration of the arcuate zone of the adrenal cortex, is probably the cause of the hyponatremia, hyperkalemia and hemoconcentration observed. Simultaneously plasma levels of urea and creatinine were elevated five- to eightfold. Due to hyperkalemia episodes with collapse, paralysis and severe electrocardiographic changes occurred several times. Two pigs died in the course of such attacks. Individuals, which transiently showed distinct clinical symptoms of intoxication, did not show any signs of disease 15 or 18 weeks later. Therefore it may be assumed, that the lesions of the adrenals, heart and skeletal muscle caused by olaquindox intoxication are reversible in some cases.
Asunto(s)
Quinoxalinas/envenenamiento , Enfermedades de los Porcinos/inducido químicamente , Animales , Proteínas Sanguíneas/análisis , Hematócrito/veterinaria , Hemoglobinas/análisis , Porcinos , Enfermedades de los Porcinos/sangreRESUMEN
A method has been developed for the isolation of bovine type II pneumocytes by enzymatic tissue dissociation and subsequent density gradient centrifugation. After mechanical defibrination, the crude cell suspension contains 71.9 +/- 26.6 X 10(7) cells (viability greater than 95%) with a type II cell purity of 39.4 +/- 10.9%. Due to their low buoyant density, bovine type II pneumocytes can be purified in a single step to 95.7 +/- 1.7% by discontinuous density gradient centrifugation on a 1.040 g/ml Percoll gradient. Isolated cells are identified by light and fluorescence microscopy that show their characteristic intracytoplasmatic surfactant granules. The fine structure of the surfactant lamellar bodies is examined in ultra thin sectioned and freeze-fractured type II pneumocytes. Isolation of bovine type II pneumocytes for the in vitro study of the surfactant system offers an alternative to the use of laboratory animals and provides an ideal system for the isolation of any desired cell number from one animal by simply increasing the size of the lung segment to be trypsinized.