RESUMEN
Previous studies have shown that our perception of stimulus properties can be affected by the emotional nature of the stimulus. It is not clear, however, how emotions affect visually-guided actions toward objects. To address this question, we used toy rats, toy squirrels, and wooden blocks to induce negative, positive, and neutral emotions, respectively. Participants were asked to report the perceived distance and the perceived size of a target object resting on top of one of the three emotion-inducing objects; or to grasp the same target object either without visual feedback (open-loop) or with visual feedback (closed-loop) of both the target object and their grasping hand during the execution of grasping. We found that the target object was perceived closer and larger, but was grasped with a smaller grip aperture in the rat condition than in the squirrel and the wooden-block conditions when no visual feedback was available. With visual feedback present, this difference in grip aperture disappeared. These results showed that negative emotion influences both perceived size and grip aperture, but in opposite directions (larger perceived size but smaller grip aperture) and its influence on grip aperture could be corrected by visual feedback, which revealed different effects of emotion to perception and action. Our results have implications on the understanding of the relationship between perception and action in emotional condition, which showed the novel difference from previous theories.
RESUMEN
Four Massachusetts-type (Mass-type) strains of infectious bronchitis coronavirus (IBV) were compared genetically with the pathogenic M41 and H120 vaccine strains using the complete genomic sequences. The results revealed that strains ck/CH/LNM/091017 and ck/CH/LDL/101212 were closely related to the H120 vaccine, which suggests that they might represent re-isolations of vaccine strains or variants of vaccine strains that have resulted from the accumulated point mutations after several passages in chickens. In contrast, strains ck/CH/LHLJ/07VII and ck/CH/LHLJ/100902 had a close genetic relationship with the pathogenic M41 strain. In addition, molecular markers have been identified that distinguish between field and vaccine (or vaccine-like) Mass-type viruses, which may be able to differentiate between field and vaccine strains for diagnostic purposes. Phylogenetic analysis, and pairwise comparison of full-length genomes and the nine genes, identified the occurrence of recombination events in the genome of strain CK/VH/LHLJ/07VII, which suggests that this virus originated from recombination events between M41- and H120-like strains at the switch site located at the 3' end of the nucleocapsid (N) genes. To our knowledge, this is the first time that evidence for the evolution and natural recombination under field conditions between Mass-type pathogenic and vaccinal IBV strains has been documented. These findings provide insights into the emergence and evolution of the Mass-type IB coronaviruses and may help to explain the emergence of Mass-type IBV in chicken flocks all over the world.
Asunto(s)
Genoma Viral , Virus de la Bronquitis Infecciosa/clasificación , Virus de la Bronquitis Infecciosa/genética , Recombinación Genética , Regiones no Traducidas 3' , Regiones no Traducidas 5' , Animales , Pollos , Orden Génico , Datos de Secuencia Molecular , FilogeniaRESUMEN
An infectious bronchitis virus (IBV), ck/CH/LZJ/111113, was isolated from a H120-vaccinated chicken which showed disease suspected of IBV infection. Neutralization testing showed that ck/CH/LZJ/111113 was distinct from either the Chinese predominant IBV LX4-type or Mass-type vaccine strains. Phylogenetic analysis confirmed that ck/CH/LZJ/111113 is of the 4/91 type; however, further extensive analyses of full-length genomes identified occurrence of recombination events. Therefore, ck/CH/LZJ/111113 originated from the recombination events between ck/CH/LDL/091022- and 4/91-like strains at three switch sites located upstream of the spike (S) glycoprotein gene, and the 3' ends of S1 and nuceocapsid (N) genes, respectively. The difference of serotypes and tissue tropisms in kidneys between ck/CH/LZJ/111113 and ck/CH/LDL/091022 may have been contributed by the uptake of the S1 gene by a ck/CH/LDL/091022-like virus from a 4/91-like strain. This recombination event took place at the 3' end of the N gene and the 3' untranslated region may account for differences in replication efficiency in tissues of chickens inoculated by the two viruses.
Asunto(s)
Pollos , Infecciones por Coronavirus/veterinaria , Virus de la Bronquitis Infecciosa/genética , Nucleocápside/genética , Enfermedades de las Aves de Corral/virología , Regiones no Traducidas 3' , Animales , Secuencia de Bases , China , Infecciones por Coronavirus/virología , Virus de la Bronquitis Infecciosa/aislamiento & purificación , Datos de Secuencia Molecular , FilogeniaRESUMEN
Fifty-six isolates of avian infectious bronchitis virus (IBV) were obtained from different field outbreaks in China in 2010, and they were genotyped by comparison with 19 reference strains in the present study. The results showed that LX4-type isolates are still the predominant IBVs circulating in chicken flocks in China, and these isolates could be grouped further into two clusters. Viruses in each cluster had favored amino acid residues at different positions in the S1 subunit of the spike protein. In addition, a recombination event was observed to have occurred between LX4- and tl/CH/LDT3/03I-type strains, which contributed to the emergence of a new strain. The most important finding of the study is the isolation and identification of Taiwan II-type (TW II-type) strains of IBV in mainland China in recent years. The genome of TW II-type IBV strains isolated in mainland China has experienced mutations and deletions, as demonstrated by comparison of the entire genome sequence with those of IBV strains isolated in Taiwan. Pathogenicity testing and sequence analysis of the 3' terminal untranslated region revealed that TW II-type IBV strains isolated in mainland China have a close relationship with the embryo-passaged, attenuated TW2296/95.
Asunto(s)
Pollos , Infecciones por Coronavirus/veterinaria , Virus de la Bronquitis Infecciosa/clasificación , Virus de la Bronquitis Infecciosa/genética , Glicoproteínas de Membrana/genética , Enfermedades de las Aves de Corral/virología , Proteínas del Envoltorio Viral/genética , Animales , Anticuerpos Antivirales/sangre , Embrión de Pollo , China , Clonación Molecular , Infecciones por Coronavirus/virología , Femenino , Variación Genética , Genotipo , Virus de la Bronquitis Infecciosa/química , Virus de la Bronquitis Infecciosa/patogenicidad , Masculino , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Análisis de Secuencia de ADN , Análisis de Secuencia de Proteína , Glicoproteína de la Espiga del Coronavirus , VirulenciaRESUMEN
Seventy-eight isolates of avian infectious bronchitis virus (IBV) were obtained from different field outbreaks in China in 2009 and genotyped with 34 reference strains. Four genotypes of IBV and three new isolates were identified by phylogenetic analysis and BLAST searches of the entire S1 gene. The results showed that most IBV strains that have circulated in China in recent years belong to the genotype of QX-like strains, and that they could be grouped further into two clusters, regardless of the level of genetic variation displayed. A study of pathogenicity that used three QX-like strains--ck/CH/LSD/091003, ck/CH/LDL/091022 and ck/CH/LJL/090330--showed that the isolates caused the most severe lesions in the kidneys and were therefore nephropathogenic strains with various levels of virulence in specific pathogen free chickens. A vaccination-challenge test that was performed using the three QX-like strains showed that the commercially available H120 vaccine did not provide sufficient protection against challenge with the QX-like isolates, as demonstrated by comparison of the clinical signs, pathological lesions and virus recovery from the trachea and kidney of unvaccinated-challenged and vaccinated-challenged birds.
Asunto(s)
Infecciones por Coronavirus/veterinaria , Virus de la Bronquitis Infecciosa/genética , Virus de la Bronquitis Infecciosa/inmunología , Filogenia , Enfermedades de las Aves de Corral/virología , Vacunas Virales , Animales , Pollos , China/epidemiología , Infecciones por Coronavirus/prevención & control , Infecciones por Coronavirus/virología , Brotes de Enfermedades/prevención & control , Brotes de Enfermedades/veterinaria , Femenino , Virus de la Bronquitis Infecciosa/aislamiento & purificación , Virus de la Bronquitis Infecciosa/patogenicidad , Vigilancia de la Población , Enfermedades de las Aves de Corral/prevención & control , Vacunación/veterinariaRESUMEN
A comprehensive study of the epidemiology and pathogenicity of infectious bronchitis virus (IBV) in China was carried out by molecular characterization of the S1 gene from 46 isolates obtained for this study and 174 reference strains isolated over a 15-year period. Nine types were found according to sequence analysis and phylogenetic study of the S1 gene. The co-circulation of multiple IBV types and the ongoing emergence of IBV variants are the epidemiological challenges in China. Factors contributing to the continual emergence include mutations, insertions and deletions in the S1 protein genes; recombination between local IBV strains circulating in chicken flocks in China; and recombination between local strains and vaccine strains. Vaccination-challenge analysis between circulating field strains and Mass-type H120 vaccine indicated the need to develop new vaccines from local IBV strains. These results also emphasize the importance of continued IBV surveillance in China.