RESUMEN
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin ß1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
Asunto(s)
Técnicas de Cultivo de Célula/métodos , Proliferación Celular , Integrina beta1/genética , Citoesqueleto de Actina/fisiología , Análisis de Varianza , Animales , Carcinoma , Línea Celular/fisiología , Fibroblastos/fisiología , Técnica del Anticuerpo Fluorescente/métodos , Humanos , Presión Hidrostática , Metilaminas , Ratones Endogámicos C57BL , Carcinoma Nasofaríngeo , Neoplasias Nasofaríngeas/patología , Cultivo Primario de Células , Ratas , Reacción en Cadena en Tiempo Real de la Polimerasa , Células Ganglionares de la Retina/citología , Células Ganglionares de la Retina/fisiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Estrés MecánicoRESUMEN
Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.