RESUMEN
A non-linear mixed-effects model is proposed to assess the impact of acarbose over time on postprandial glycaemia in a single rat. The model is based on two compartments, one representing the entry of glucose in the blood and the other its exit. The rat was submitted to two treatments: ingestion of starch and ingestion of starch plus acarbose. The model showed great suitability, with inferences on the behavior of glucose levels in response to treatments and supplying a richer description than just the area under the curve. The marginal curves for the two treatments are similar during the first moments; however, after reaching the peak of glucose concentration, they progressively became separate due to acarbose treatment and reached the initial levels more quickly. The proposed model, albeit with a single sample unit, showed similar results to those with larger samples; in other words, acarbose significantly attenuates glycaemia after ingestion of starch.
Neste estudo, foi proposto um modelo não linear de efeitos mistos para verificar o impacto da acarbose ao longo do tempo na glicemia pós -prandial de um único rato. Adotou-se um modelo de dois compartimentos: um representando a entrada de glicose no sangue e outro, a saída. O rato foi submetido a dois tratamentos: ingestão de amido e de amido com adição de acarbose. O modelo proposto apresentou um ótimo ajuste, permitindo fazer inferências do comportamento da glicose para os tratamentos e fornecendo uma descrição muito mais rica do que simplesmente a área sob a curva. As curvas marginais para os dois tratamentos foram semelhantes nos primeiros tempos observados, porém, após o pico de concentração de glicose, elas se distanciaram progressivamente com o tratamento da acarbose atingindo os níveis iniciais mais rapidamente. O modelo adotado, com uma única unidade amostral, mostrou resultados similares a outros estudos com maior número de unidades amostrais, isto é, a acarbose pode atenuar consideravelmente a glicemia após ingestão de amido.
Asunto(s)
Ratas , Acarbosa , Diabetes Mellitus , GlucosaRESUMEN
The impact of cyclodextrins (CDs) on postprandial glycemic response employing the real-time continuous glucose monitoring system (RT-CGMS) was investigated. For this purpose, α-CD, ß-CD, γ-CD, HP-ß-CD, curdlan, and dextrin at doses of 10 and 100 mg/kg were orally administered in rats. The RT-CGMS was efficient to evaluate the impact of CDs on postprandial glycemia. The results showed that α-CD, ß-CD, dextrin, and curdlan did not reduce the glycemic response after the administration of starch. In contrast, the HP-ß-CD (100 mg/kg) attenuated the rise in glycemia. Moreover, the γ-CD blunts the postprandial glycemic excursion at doses of 10 and 100 mg/kg. Therefore, γ-CD could attenuate the rise in glycemia promoted by oral administration of starch. Considering that the treatment of postprandial hyperglycemia is necessary to prevent type 2 diabetes, this study opens the perspective of better control of postprandial glycemia by the addition of γ-CD in food.