RESUMEN
Cytoenzymochemical investigations were performed on the leukocytes and platelets from 2,782 workers occupationally exposed to benzene, vinyl chloride or carbon disulphide, in view of detecting the eventual occurrence of cytologic metabolic disorders induced by these chemical substances. The cytomorphologic aspects and the cytoenzymochemical parameters studied, such as the leukocyte alkaline phosphatase, endolymphocytic glycogen, lymphocytic nucleolar RNA amounts (revealed by the lymphocyte nucleologram), as well as the platelet counts have shown some abnormal values, whose severity was related to the time of exposure to toxic chemicals. The results obtained demonstrate the utility of such tests for an early detection of some metabolic disorders of the endolymphocytic proteins, carbohydrates or lipids, in view of preventing the occurrence of more severe pathologic consequences.
Asunto(s)
Benceno/efectos adversos , Plaquetas/efectos de los fármacos , Disulfuro de Carbono/efectos adversos , Leucocitos/efectos de los fármacos , Cloruro de Vinilo/efectos adversos , Compuestos de Vinilo/efectos adversos , Fosfatasa Ácida/sangre , Fosfatasa Alcalina/sangre , Recuento de Células Sanguíneas , Plaquetas/enzimología , Exposición a Riesgos Ambientales , Glucógeno/sangre , Histocitoquímica , Humanos , Leucocitos/enzimología , Factores de TiempoRESUMEN
A group of 100 workers exposed to vinyl chloride (VC) (mean duration of exposure-13.2 years) were subjected to a complex medical examination for the detection of eventual alterations of the biologic parameters, induced by occupational exposure. Leukocyte cytoenzymochemical determinations (lymphocytic RNA--Plenert's method modified by Micu and glycogen--Hotchkiss McManus method), which might give some information on two important metabolic processes--of proteins and of carbohydrates--were performed in peripheral blood smears. Lymphocytic nucleologram and the relative and absolute nucleolar area showed an increase of endolymphocytic RNA, due to the high percentage of binucleolated cells and of spherical macronucleoli. The increase of the endolymphocytic glycogen score in parallel with the duration exposure was in correlation with high titers of serum gamma GT.
