RESUMEN
Significant fractions of eukaryotic genomes give rise to RNA, much of which is unannotated and has reduced protein-coding potential. The genomic origins and the associations of human nuclear and cytosolic polyadenylated RNAs longer than 200 nucleotides (nt) and whole-cell RNAs less than 200 nt were investigated in this genome-wide study. Subcellular addresses for nucleotides present in detected RNAs were assigned, and their potential processing into short RNAs was investigated. Taken together, these observations suggest a novel role for some unannotated RNAs as primary transcripts for the production of short RNAs. Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes. These data support a highly interleaved organization of the human transcriptome.
Asunto(s)
Genoma Humano , Precursores del ARN/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN/genética , Transcripción Genética , Animales , Línea Celular Tumoral , Núcleo Celular/metabolismo , Citosol/metabolismo , Exones , Expresión Génica , Genoma , Células HeLa , Humanos , Ratones , Regiones Promotoras Genéticas , ARN/metabolismo , Precursores del ARN/metabolismo , Sintenía , Regiones Terminadoras GenéticasRESUMEN
Sites of transcription of polyadenylated and nonpolyadenylated RNAs for 10 human chromosomes were mapped at 5-base pair resolution in eight cell lines. Unannotated, nonpolyadenylated transcripts comprise the major proportion of the transcriptional output of the human genome. Of all transcribed sequences, 19.4, 43.7, and 36.9% were observed to be polyadenylated, nonpolyadenylated, and bimorphic, respectively. Half of all transcribed sequences are found only in the nucleus and for the most part are unannotated. Overall, the transcribed portions of the human genome are predominantly composed of interlaced networks of both poly A+ and poly A- annotated transcripts and unannotated transcripts of unknown function. This organization has important implications for interpreting genotype-phenotype associations, regulation of gene expression, and the definition of a gene.
Asunto(s)
Cromosomas Humanos/genética , Genoma Humano , ARN Mensajero/análisis , Transcripción Genética , Línea Celular , Línea Celular Tumoral , Núcleo Celular/metabolismo , Cromosomas Humanos Par 13/genética , Cromosomas Humanos Par 14/genética , Cromosomas Humanos Par 19/genética , Cromosomas Humanos Par 20/genética , Cromosomas Humanos Par 21/genética , Cromosomas Humanos Par 22/genética , Cromosomas Humanos Par 6/genética , Cromosomas Humanos Par 7/genética , Cromosomas Humanos X/genética , Cromosomas Humanos Y/genética , Biología Computacional , Citosol/metabolismo , ADN Complementario , ADN Intergénico , Exones , Femenino , Humanos , Intrones , Masculino , Datos de Secuencia Molecular , Técnicas de Amplificación de Ácido Nucleico , Análisis de Secuencia por Matrices de Oligonucleótidos , Mapeo Físico de Cromosoma , Empalme del ARNRESUMEN
In this report, we have achieved a richer view of the transcriptome for Chromosomes 21 and 22 by using high-density oligonucleotide arrays on cytosolic poly(A)(+) RNA. Conservatively, only 31.4% of the observed transcribed nucleotides correspond to well-annotated genes, whereas an additional 4.8% and 14.7% correspond to mRNAs and ESTs, respectively. Approximately 85% of the known exons were detected, and up to 21% of known genes have only a single isoform based on exon-skipping alternative expression. Overall, the expression of the well-characterized exons falls predominately into two categories, uniquely or ubiquitously expressed with an identifiable proportion of antisense transcripts. The remaining observed transcription (49.0%) was outside of any known annotation. These novel transcripts appear to be more cell-line-specific and have lower and less variation in expression than the well-characterized genes. Novel transcripts were further characterized based on their distance to annotations, transcript size, coding capacity, and identification as antisense to intronic sequences. By RT-PCR, 126 novel transcripts were independently verified, resulting in a 65% verification rate. These observations strongly support the argument for a re-evaluation of the total number of human genes and an alternative term for "gene" to encompass these growing, novel classes of RNA transcripts in the human genome.
Asunto(s)
Cromosomas Humanos Par 21/genética , Cromosomas Humanos Par 22/genética , ARN/genética , Transcripción Genética/genética , Línea Celular , Línea Celular Tumoral , Mapeo Cromosómico/métodos , ADN de Neoplasias/genética , Perfilación de la Expresión Génica/métodos , Genes/genética , Genes Relacionados con las Neoplasias/genética , Humanos , Células Jurkat/química , Células Jurkat/metabolismo , Datos de Secuencia Molecular , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Sondas de Oligonucleótidos/genética , ARN Mensajero/genéticaRESUMEN
Using high-density oligonucleotide arrays representing essentially all nonrepetitive sequences on human chromosomes 21 and 22, we map the binding sites in vivo for three DNA binding transcription factors, Sp1, cMyc, and p53, in an unbiased manner. This mapping reveals an unexpectedly large number of transcription factor binding site (TFBS) regions, with a minimal estimate of 12,000 for Sp1, 25,000 for cMyc, and 1600 for p53 when extrapolated to the full genome. Only 22% of these TFBS regions are located at the 5' termini of protein-coding genes while 36% lie within or immediately 3' to well-characterized genes and are significantly correlated with noncoding RNAs. A significant number of these noncoding RNAs are regulated in response to retinoic acid, and overlapping pairs of protein-coding and noncoding RNAs are often coregulated. Thus, the human genome contains roughly comparable numbers of protein-coding and noncoding genes that are bound by common transcription factors and regulated by common environmental signals.
