RESUMEN
Meiotic chromosomes in human oocytes are packaged differently than in spermatocytes at the pachytene stage of meiosis I, when crossing-over takes place. Thus the meiosis-specific pairing structure, the synaptonemal complex (SC), is considerably longer in oocytes in comparison to spermatocytes. The aim of the present study was to examine the influence of this length factor on meiotic recombination in male and female human germ cells. The positions of crossovers were identified by the DNA mismatch repair protein MLH1. Spermatocytes have approximately 50 crossovers per cell in comparison to more than 70 in oocytes. Analyses of inter-crossover distances (and presumptively crossover interference) along SCs suggested that while there might be inter-individual variation, there was no consistent difference between sexes. Thus the higher rate of recombination in human oocytes is not a consequence of more closely spaced crossovers along the SCs. The rate of recombination per unit length of SC is higher in spermatocytes than oocytes. However, when the so-called obligate chiasma is excluded from the analysis, then the rates of recombination per unit length of SC are essentially identical in the two sexes. Our analyses indicate that the inter-sex difference in recombination is largely a consequence of the difference in meiotic chromosome architecture in the two sexes. We propose that SC length per se, and therefore the size of the physical platform for crossing-over (and not the DNA content) is the principal factor determining the difference in rate of recombination in male and female germ cells. A preliminary investigation of SC loop size by fluorescence in situ hybridization (FISH) indicated loops may be shorter in oocytes than in spermatocytes.
Asunto(s)
Intercambio Genético/genética , Intercambio Genético/fisiología , Oocitos/metabolismo , Caracteres Sexuales , Espermatocitos/metabolismo , Complejo Sinaptonémico/metabolismo , Proteínas Adaptadoras Transductoras de Señales , Proteínas Portadoras , Cromosomas Humanos/genética , Cromosomas Humanos/metabolismo , Femenino , Genoma Humano , Genómica , Humanos , Hibridación Fluorescente in Situ , Masculino , Meiosis , Homólogo 1 de la Proteína MutL , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Proteínas NuclearesRESUMEN
We investigated the behaviour of centromeres and distal telomeres during the initial phases of female meiosis in mice. In particular, we wished to determine whether clustering of centromeres and telomeres (bouquet formation) played the same crucial role in homologous chromosome pairing in female meiosis as it does in the male. We found that synapsis (intimate homologous chromosome pairing) is most frequently initiated in the interstitial regions of homologous chromosomes, apparently ahead of the distal regions. The proximal ends of the chromosomes appear to be disfavoured for synaptic initiation. Moreover, initiation of synapsis occurred in oocytes that showed little or no evidence of bouquet formation. A bouquet was present in a substantial proportion of cells at mid to late zygotene, and was still present in some pachytene oocytes. This pattern of bouquet formation and pairing initiation is in stark contrast to that previously described in the male mouse. We propose that although dynamic movements of centromeres and telomeres to form clusters may facilitate alignment of homologues or homologous chromosome segments during zygotene, in the female mouse positional control of synaptic initiation is dependent on some other mechanism.
Asunto(s)
Centrómero/fisiología , Emparejamiento Cromosómico/fisiología , Meiosis/fisiología , Oocitos/citología , Telómero/fisiología , Animales , Criopreservación , Femenino , Técnica del Anticuerpo Fluorescente , Hibridación Fluorescente in Situ , Masculino , Ratones , Ratones Endogámicos C3H , Modelos Genéticos , Caracteres SexualesRESUMEN
The influence of trisomy on meiotic chromosome association and synapsis was studied in oocytes of two trisomy 21 fetuses. The patterns of association of the three chromosomes 21 were determined by analysis of late zygotene to early diplotene fetal oocytes after immunofluorescent staining of synaptonemal complexes. The identity of chromosome 21 was confirmed using FISH with either a whole chromosome 21 paint or an alpha-satellite DNA repeat probe. In both fetuses, a wide variety of configurations was present at pachytene. The most common configurations were a trivalent (35.5% and 51.6% of analyzable cells) and a bivalent plus univalent (62.9% and 45.2%). These different frequencies between the fetuses were not significant. Trivalents showed either triple synapsis or double synapsis with pairing-partner switches. The extent of triple synapsis varied from a short segment, either terminal or interstitial, to the whole chromosome length. Through use of immunofluorescent staining of the centromeres, we identified novel types of abnormal chromosome behavior in trisomy 21 fetal oocytes. Thus, we found that 6/41 trivalents had one of the chromosomes associated "out of register," i.e., in a nonhomologous fashion, with its two homologs. Likewise, we found three cells with bivalent plus univalent configurations, in which the univalent showed self-synapsis. The presence of three copies of chromosome 21 therefore results not only in the formation of complex and highly variable synaptic associations but also causes a significant increase in the occurrence of nonhomologous synapsis in human fetal oocytes.
Asunto(s)
Síndrome de Down/genética , Feto/fisiología , Meiosis/fisiología , Oocitos/citología , Aborto Inducido , Femenino , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Embarazo , Cigoto/citologíaRESUMEN
Analysis of a radiation-induced minichromosome using classical cytogenetic techniques and fluorescence in situ hybridization (FISH) has shown this element to be composed primarily, if not entirely, of repeat sequence DNAs that are common to the ends of all chromosomes in the mouse genome. This novel chromosome has been used to examine further the role of centromeric and telomeric DNA sequences in the initiation of homologous chromosome pairing and synapsis in female germ cells. In pachytene oocytes of a fetus carrying two copies of the minichromosome, it was found that these elements were collocalized, but had not undergone synapsis, in half of the cells analysed. The minichromosomes formed a synaptonemal complex in approximately 14% of oocytes. Overall, therefore, the minichromosomes showed a surprising ability to achieve the first phase of homologous chromosome pairing, i.e. collocalization, despite their dearth of chromosome-specific DNA sequences. It is suggested that this collocalization largely results from the tendency of mouse chromosome ends to form centromeric and telomeric clusters at zygotene. The observations here provide support for the proposition that clustering of chromosome ends in early meiosis fosters pairing interactions and synapsis.
Asunto(s)
Cromosomas/fisiología , Meiosis , Secuencias Repetitivas de Ácidos Nucleicos/fisiología , Complejo Sinaptonémico , Animales , Bandeo Cromosómico , Sondas de ADN , Diploidia , Femenino , Feto , Heterocromatina , Hibridación Fluorescente in Situ , Ratones , Microscopía Electrónica , No Disyunción Genética , Oocitos/ultraestructura , TelómeroRESUMEN
Nine regions on six mouse autosomes are subject to imprinting and uniparental inheritance of any one of these regions results in mice with phenotypic anomalies. So far on distal Chromosome (Chr) 2 there is a unique imprinting region between 2H3 and 2H4 associated with two behavioural disorders and neonatal lethality. A maternally imprinted gene, Nnat, has been identified which is expressed in the nervous system and maps to distal Chr 2. Nnat has been excluded as a candidate for either or both the behavioural phenotypes as it lies proximal to the 2H3-2H4 imprinting region. Here we have mapped Nnat to band 2H1 which is at least 18 Mb proximal to the previously described imprinting region. It maps close to agouti, some alleles of which show differential expression according to parental origin. The localisation of Nnat to band H1 confirms and refines the map location of a second imprinting region on mouse Chr 2.
Asunto(s)
Proteínas Morfogenéticas Óseas , Cromosomas/genética , Genes/genética , Impresión Genómica/genética , Proteínas de la Membrana/genética , Proteínas del Tejido Nervioso/genética , Animales , Fragilidad Cromosómica , Mapeo Cromosómico , Femenino , Factor 5 de Diferenciación de Crecimiento , Sustancias de Crecimiento/genética , Hibridación Fluorescente in Situ , Isoenzimas/genética , Masculino , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Muridae , Fosfolipasa C gamma , Translocación Genética , Fosfolipasas de Tipo C/genéticaRESUMEN
Two factors postulated to influence the meiotic behaviour of reciprocal translocations were investigated. Firstly, variation in the length of translocated and non-translocated segments was studied in male mice using four different rearrangements involving chromosomes 2 and 4. Secondly, sex-related effects were analysed through comparison of the meiotic behaviour of two translocations in male and female germ cells. In the first part of the study, primary and secondary spermatocytes of male mice carrying a translocation [T(2;4)1Ca, T(2;4)13H, T(2;4)1Sn, or T(2;4)1Go] were screened. Each rearrangement had different proportions of cells with ring and chain quadrivalents at metaphase I; the T(2;4)1Sn heterozygote also had a high rate (45%) of translocation bivalents. In general, the translocations had elevated chiasma frequencies in the rearranged chromosomes compared with structurally normal chromosomes 2 and 4, although the extent of the effect varied. Each rearrangement produced a different array of segregation products at metaphase II, reflecting their contrasting frequencies of multivalent configurations at metaphase I. Comparison of chromosome behaviour at metaphase I and II suggested that certain configurations tended to adopt particular orientations. However, it was also clear that such correlations were imprecise and that other factors, possibly the exact positions of chiasmata, also played a role in multivalent orientation. Two rearrangements, T(2;4)1Go and T(7;16)67H, were analysed in female mice. The frequencies of the various multivalent types at metaphase I differed from those in male carriers of these rearrangements owing to an increased chiasma frequency in oocytes in some of the pairing segments. Not surprisingly, the segregation products seen in metaphase II cells showed some differences from the pattern recorded in male germ cells. For T(2;4)1Go, the sex-related difference in segregation patterns resulted in a diminished expectation of genetically imbalanced gametes, although this was not the case for T(7;16)67H.
Asunto(s)
Mapeo Cromosómico , Translocación Genética , Animales , Cruzamientos Genéticos , Femenino , Hibridación Fluorescente in Situ , Cariotipificación , Masculino , Metafase , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos , Modelos Genéticos , Óvulo/citología , Espermatozoides/citologíaRESUMEN
The initiation and progression of homologous chromosome pairing at meiosis were investigated in female mice. The proximal end of the X chromosome was identified in fetal oocytes using fluorescence in situ hybridisation with the repeat copy probe 70-38. The X centromeres appeared to be randomly positioned in the nuclei from pre-meiotic interphase to leptotene. The observations indicated no pre-synaptic association for the proximal end of the X chromosome. There was a significant increase in the number of paired X centromeres from mid-zygotene to late zygotene. The proximal end of the X chromosome is therefore a generally late pairing region with no significant association seen before mid-zygotene. The centromeric heterochromatin of all chromosomes could be seen to associate into varying numbers of clusters during pre-leptotene through to pachytene. These clusters do not seem to be directly involved in bringing homologues together, as X centromeres did not consistently localise to the same cluster.
Asunto(s)
Meiosis , Cromosoma X , Animales , Embrión de Mamíferos/fisiología , Femenino , Edad Gestacional , Soluciones Hipotónicas/farmacología , Masculino , Ratones , Ratones Endogámicos C3H , Microscopía Fluorescente , Oocitos/efectos de los fármacos , Oocitos/fisiología , Profase/genéticaRESUMEN
Meiotic chromosome behaviour was investigated in male mice heterozygous for the translocation T(7;16)67H. At metaphase I, chain-of-four quadrivalents were present in approximately 80% of the spermatocytes; the bulk of remaining cells contained a ring quadrivalent, with only a few having either a trivalent plus univalent configuration or two bivalents. A low rate of non-disjunction, approximately 5%, was found through analysis of C-banded metaphase II spermatocytes. Using fluorescence in situ hybridization with differentially labelled whole chromosome paints, a wide array of segregation products were observed at metaphase II, depending on whether they arose from alternate, adjacent I, adjacent II orientation at metaphase I or were uninformative for alternative/adjacent I because of the presence of a chiasma in an interstitial pairing segment. Some 62% of the cells fell into this latter category, with only small proportions clearly arising through alternate (1.8%) or adjacent I (0.7%) orientations. Approximately 30% of the cells contained the products of adjacent II orientation. Consideration of the data suggested that most of these cells arose from metaphase I cells that contained a chain quadrivalent. Ring quadrivalents appeared predominantly to orientate in an alternate/adjacent I manner.
Asunto(s)
Meiosis , Ratones/genética , Translocación Genética , Animales , Bandeo Cromosómico , Hibridación Fluorescente in Situ/métodos , Masculino , MetafaseRESUMEN
The incidences of X-ray induced numerical and structural chromosome anomalies were screened in a range of developmental stages from metaphase II oocytes through to post-implantation embryos. Following 1 Gy of acute X-rays to immediately preovulatory stage oocytes, the rate of hyperploidy (chromosome gain) was found to be elevated over levels in unirradiated controls, at metaphase II, in 1-cell and 3.5 day pre-implantation embryos but not in 8.5 day post-implantation foetuses. In the latter, however, the frequency of mosiacism was significantly increased. A similar response of an increase in mosaicism but not in hyperploidy in 8.5 day post-implantation embryos was also found after irradiation of dictyate stage oocytes with 4 Gy of acute X-rays. Significantly elevated frequencies of structural chromosome anomalies were present in metaphase II oocytes and pre-implantation embryonic stages, but could not be detected in block-stained chromosome preparations from 8.5 day post-implantation foetuses. However, analysis of chromosome preparations after G-banding showed that almost 14% of 14.5 day foetuses carried a chromosome rearrangement after 1 Gy of X-rays to immediately preovulatory stage oocytes. Overall, our data indicate that the presence of radiation-induced chromosome gains are incompatible with embryonic survival but that a proportion of embryos with structural chromosome damage develop past mid-gestation. These latter embryos are therefore potentially capable of contributing to the genetic burden of the next generation.
Asunto(s)
Aberraciones Cromosómicas , Oocitos/efectos de la radiación , Animales , Desarrollo Embrionario/efectos de la radiación , Desarrollo Embrionario y Fetal/efectos de la radiación , Femenino , Metafase , Ratones , Ratones Endogámicos C3H , No Disyunción Genética , EmbarazoRESUMEN
The rates of X-ray induced loss of chromosome 19 in mouse oocytes were investigated in 2 experiments using a genetic complementation test. After 1 Gy of acute X-rays to immediately preovulatory stage oocytes, chromosome 19 loss was estimated to have occurred in 1.68% of cells. In comparison, after 4 Gy of acute X-rays to dictyate stage oocytes, the rate was estimated at 1.18%. The slightly higher rate of chromosome loss in the former cell stage after a smaller dose of radiation reflects the known increased radiosensitivity of mouse oocytes in the period shortly before ovulation. Comparison of the observations here for chromosome 19 with published data for chromosome 1 suggests that chromosome length is one of the principal factors in determining the initial rate of induced loss in mouse oocytes. Ten dominant visible mutations were recovered among 1674 offspring following irradiation of preovulatory oocytes, and 8 in 2025 offspring after treatment of dictyate cells. Nine dominant mutations were karyotyped, 5 of these were found to be associated with a visible chromosome rearrangement. The data obtained in the present study show that radiation-induced chromosome anomalies in female germ cells are not all filtered out by prenatal embryonic death but that a proportion has the potential to contribute to the genetic burden of the next generation.
Asunto(s)
Deleción Cromosómica , Oocitos/efectos de la radiación , Animales , Relación Dosis-Respuesta en la Radiación , Femenino , Genes Dominantes , Masculino , Ratones , Ratones Endogámicos C3HRESUMEN
Chiasma frequencies were analysed and chiasma positions measured in diakinesis/metaphase I autosomal bivalents from oocytes and spermatocytes of F1 hybrid C3H/HeHx101/H mice. Twenty chromosome size ranks, including the presumptive X bivalent, could be distinguished in oocytes, and nineteen autosomal ranks plus the XY pair spermatocytes. Overall, mean cell chiasma frequencies of the two sexes did not differ significantly once the contribution of the presumptive X bivalent and the XY pair were taken into account. Sex related differences in chiasma distribution patterns were evident, however. In monochiasmate bivalents, the chiasma was most commonly located interstitially in oocytes while in spermatocytes it could be either interstitial or distal. In dichiasmate bivalents, the chiasmata tended to be more centrally located in oocytes than in spermatocytes. Minimum inter-chiasma distances did not appear to show any great variation in chromosome pairs of different sizes, however, mean inter-chiasma distances did increase with the bivalent length. The minimum-inter chiasma distance data suggest that chiasma interference is complete over a chromosomal segment equating to approximately 60Mb. Measurement of the positions of chiasmata along chromosome arms open up the possibility of producing chiasma-based genetic maps for all the autosomes of the mouse.
Asunto(s)
Mapeo Cromosómico , Intercambio Genético , Ratones Endogámicos C3H/genética , Caracteres Sexuales , Animales , Femenino , Masculino , Meiosis , Ratones , Oocitos/ultraestructura , Espermatocitos/ultraestructuraRESUMEN
The X chromosome pair was identified in diakinesis/metaphase I stage mouse oocytes using a repeat sequence DNA probe and fluorescence in situ hybridisation. Chiasma positions along the X bivalent were measured in 57 oocytes from 4 females. Overall, our observations showed that while there were no obvious "hotspots" for chiasma formation along the X chromosome, there was a tendency to favour the distal end. Minimum inter-chiasma distances were substantial indicating the occurrence of strong genetic interference. Estimates of both genetic distances and recombination fractions for any interval along the chromosome can be calculated from the chiasma data. The average chiasma frequency for the X bivalent was 1.37 giving an estimated total genetic map length of 68.5 cM. In general, the pattern of chiasma distribution along the X chromosome resembled that anticipated from recombination distances in published consensus linkage maps. There were, however, some intriguing differences between the two approaches. The reason for these discrepancies are unknown but may be related to lack of precision in cytogenetic mapping of loci, inter-strain and/or interspecies differences in the genetic controls over the distribution of crossover events. One advantage of the chiasma analysis approach is its suitability for investigating these problems.
Asunto(s)
Ratones/genética , Oocitos/citología , Cromosoma X , Animales , Células Cultivadas , Cromátides/fisiología , Cromátides/ultraestructura , Bandeo Cromosómico , Mapeo Cromosómico , Sondas de ADN , Femenino , Ligamiento Genético , Marcadores Genéticos , Metafase , Ratones Endogámicos C3H , Ratones Endogámicos , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
The questions of whether closely spaced crossovers could be misidentified as single chiasmata or could cancel out each other and whether exchange could occur without chiasma formation were examined in Locusta migratoria. Monochiasmate bivalents that showed differential sister chromatid staining following bromodeoxyuridine incorporation were screened for their patterns of label distribution. Half of the chiasmata were associated with an exchange between dark and lightly stained chromatids, as expected if recombination involved any two non-sister chromatids chosen at random. Two variant types of label distribution were also seen: approximately 10% of all monochiasmate bivalents had an anomalous distribution of dark and light chromatids around the chiasma, and in three of the 1365 bivalents screened a second type of anomalous pattern was observed for the first time, in which all four chromatids had a label exchange at the chiasma. The observed incidence of the latter was considerably less than expected if they originated through a four-strand double crossover with closely positioned exchanges. Analysis of label distribution patterns in monochiasmate bivalents did not produce evidence of the other configurations expected if two closely spaced exchanges could be misidentified as single chiasmata or could cancel one another out so as not to form a chiasma. We conclude that analysis of chiasma frequencies and distributions offers an accurate means of assessing recombination in organisms with favourable cytology.
Asunto(s)
Cromosomas/genética , Intercambio de Cromátides Hermanas/genética , Animales , Bromodesoxiuridina/metabolismo , Saltamontes , Masculino , Recombinación Genética/genéticaRESUMEN
Chromosomes from antigen stimulated B-cells from spleens of inbred mice have been separated using flow cytometry into 18 distinguishable peaks. Using locus-specific oligonucleotides and fluorescence in situ hybridization to banded metaphase spreads, 15 individual chromosomes were identified: 1, 2, 3, 6, 7, 8, 9, 11, 12, 16, 17, 18, 19, X and Y. The remaining six chromosomes, occurring as pairs in three peaks, 4 with 5, 10 with 13, and 14 with 15, were resolved by flow sorting chromosomes from mice carrying an appropriate homozygous translocation and 4, 5 and 14 have been isolated in this way. This is the first demonstration of how a complete set of mouse chromosome paints can be produced.
Asunto(s)
Cromosomas/ultraestructura , Citometría de Flujo/métodos , Hibridación Fluorescente in Situ/métodos , Ratones/genética , Animales , Secuencia de Bases , Cartilla de ADN/genética , Sondas de ADN/genética , Masculino , Ratones Endogámicos C3H , Técnicas de Sonda Molecular , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Translocación GenéticaRESUMEN
The influence of X-autosome Robertsonian (Rb) translocation hemizygosity on meiotic chromosome behaviour was investigated in male mice. Two male fertile translocations [Rb(X.2)2Ad and Rb(X.9)6H] and a male sterile translocation [Rb(X.12)7H] were used. In males of all three Rb translocation types, the acrocentric homologue of the autosome involved in the rearrangement regularly failed at pachytene to pair completely with its partner in the Rb metacentric. The centric end of the acrocentric autosome was found regularly to associate either with the proximal end of the Y chromosome or with the ends of nonhomologous autosomal bivalents; the proportions of cells with such configurations varied between pachytene substages and genotypes. Various other categories of synaptic anomaly, such as nonhomologous synapsis, foldback pairing and interlocks, affected the sex chromosome multivalent in a substantial proportion of cells. In one of the Rb(X.12)7H males screened, an unusual, highly aneuploid spermatocyte that contained trivalent and bivalent configurations was found. Rb translocation hemizygosity did not appear to increase to a significant extent the incidence of X-Y pairing failure at pachytene, although the incidence was elevated at metaphase I in Rb(X.12)7H animals. Overall, a comparison of the frequencies and types of chromosome pairing anomalies did not suggest that these were important factors in the aetiology of infertility in males carrying the Rb(X.12)7H translocation.
Asunto(s)
Espermatocitos/química , Translocación Genética/genética , Cromosoma X , Animales , Masculino , Ratones , Recuento de Espermatozoides , Espermatozoides/citología , Espermatozoides/ultraestructuraRESUMEN
Data from studies on radiation- and chemically-induced chromosome aberrations in mouse oocytes have been summarized. An attempt has been made to assess the relative sensitivity to mutagenic agents of female and male germ cells through comparison of observations from mutation studies of female and male mice. No unequivocal evidence of a mutagenic effect limited to a single sex could be found in the cytogenetic data, although differences in relative germ cell sensitivity could be inferred for ionizing radiation and some chemicals. However, the pattern of inter-sex variations was not consistent: for example, irradiation of dictyate oocytes yielded a lower rate of heritable chromosome translocations than the same dose to spermatogonia; in contrast, some chemicals, such as mitomycin C, yielded a larger incidence of chromosome anomalies after treatment of dictyate oocytes than spermatogonia. Overall, the limitations in quality and quantity of cytogenetic data, and the uncertainties associated with comparing information obtained in disparate assays, place severe constraints on the use of observations on induced chromosome aberrations to assess the relative sensitivities of female and male germ cells to environmental mutagens.
Asunto(s)
Aberraciones Cromosómicas , Mutágenos/toxicidad , Oocitos/efectos de los fármacos , Oocitos/efectos de la radiación , Traumatismos Experimentales por Radiación/genética , Animales , Femenino , Masculino , Ratones , Espermatogonias/efectos de los fármacos , Espermatogonias/efectos de la radiaciónRESUMEN
Two new X-autosome Robertsonian (Rb) translocations, Rb(X.9)6H and Rb(X.12)7H, were found during the course of breeding the Rb(X.2)2Ad rearrangement at Harwell. The influence of these new Rbs on meiotic chromosome segregation was investigated in hemizygous males and heterozygous females and compared to that of Rb(X.2)2Ad. Screening of metaphase II spermatocytes gave incidences of sex chromosome aneuploidy of 9.2% in Rb(X.2)6H/Y and 9.6% in Rb(X.9)2Ad/Y males; no metaphase II cells were present in the testes of the Rb(X.12)7H/Y males examined and no males with this karyotype have so far proved fertile. In breeding tests, 5% of the progeny of Rb(X.2)2Ad/Y males were sex chromosome aneuploids compared to 10% of the Rb(X.9)6H/Y offspring. The difference was not significant, however. Cytogenetic analyses of metaphase II stage oocytes showed elevated rates of hyperhaploidy (n + 1) in Rb heterozygous females over chromosomally normal mice: 4.2% for Rb(X.2)2Ad/+; 2.1% for Rb(X.9)6H/+; 2.2% for Rb(X.12)7H/+ and 1.1% for normal females. There was, however, no statistically significant difference in the rates of hyperhaploidy between the three different Rb types, nor overall between Rb/+ and normal females. Karyotypic analyses of liveborn offspring of Rb heterozygous females revealed low incidences of X0 animals but no other type of sex chromosome aneuploidy. Intercrosses of heterozygous females and hemizygous males yielded 5.5% aneuploidy for Rb(X.2)2Ad and 5.4% for Rb(X.9)6H. In heterozygous females, there was evidence from the metaphase II and breeding test data for all three rearrangements, of preferential segregation of the Rb metacentric to the polar body resulting in a deficiency of cells and progeny carrying a translocation chromosome.
Asunto(s)
Translocación Genética , Cromosoma X , Aneuploidia , Animales , Bandeo Cromosómico , Femenino , Heterocigoto , Cariotipificación , Masculino , Meiosis , Ratones , No Disyunción GenéticaRESUMEN
The relative sensitivities of dictyate oocytes from young and old female mice to radiation-induced chromosome damage were examined in 2 separate experiments. Firstly, females were given either 2 or 4 Gy of X-rays and metaphase I stage oocytes collected 16.5 days later. Analysis of these cells showed dose-related increases in chromosome aberrations in both age groups. The response was significantly greater in oocytes of older females. In the second experiment, females were given 4 Gy of X-rays and metaphase I stage oocytes collected 3.5 days later. Again, a significantly larger frequency of aberrations was present in cells from older animals. Overall, these 2 experiments provide unambiguous evidence that the radiosensitivity of mouse dictyate oocytes increases with advancing maternal age.
Asunto(s)
Aberraciones Cromosómicas , Edad Materna , Oocitos/efectos de la radiación , Animales , Femenino , Ratones , Rayos X/efectos adversosRESUMEN
Karyotypic investigation of a phenotypically normal but sterile male mouse showed the presence of an XYY sex chromosome constitution. The synaptic behaviour of the three sex chromosomes was examined in 65 pachytene cells. The sex chromosomes formed a variety of synaptic configurations: an XYY trivalent (40%); an XY bivalent and Y univalent (38.5%); an X univalent and YY bivalent (13.8%); or X, Y, Y univalence (7.7%). There was considerable variation in the extent of synapsis and some of the associations clearly involved nonhomologous pairing. These observations have been compared with previously published information on chromosome configurations at metaphase I from other XYY males.
Asunto(s)
Meiosis , Espermatocitos/metabolismo , Cromosoma X/ultraestructura , Cariotipo XYY/genética , Cromosoma Y/ultraestructura , Animales , Femenino , Cariotipificación , Masculino , RatonesRESUMEN
The ability of low doses of X-rays to induce numerical and structural chromosome anomalies in immature oocytes was examined in two experiments. In the first, 10-11-day-old females were given 0.1 or 0.2 Gy of X-rays and sampled at intervals up to 32 weeks later. In the second, 4-5-week-old females were given 0.1 Gy of X-rays and sampled up to 36 weeks post-irradiation. Chromosome anomalies were assessed in metaphase II oocytes. In the first experiment, there was evidence of dose-related increases in both hyperhaploidy (n + 1) and structural chromosome anomalies. In the second experiment, only the frequency of structural chromosome anomalies was found to increase consistently after irradiation. There was no indication that radiation-induced depletion of the oocyte population was associated with an early onset of the maternal age effect on nondisjunction.