RESUMEN
Here we introduce the human induced pluripotent stem cell lines (hiPSCs), HIMRi002-A and HIMRi003-A, generated from cultured dermal fibroblasts of 61-year-old (HIMRi002-A) and 38-year-old (HIMRi003-A) female patients, carrying a known heterozygous pathogenic variant (p.A46T) in the Caveolin 3 (CAV3) gene, via lentiviral expression of OCT4, SOX2, KLF4 and c-MYC. HIMRi002-A and HIMRi003-A display typical embryonic stem cell-like morphology, carry the p.A46T CAV3 gene mutation, express several pluripotent stem cell markers, retain normal karyotype (46, XX) and can differentiate in all three germ layers. We postulate that the HIMRi002-A and HIMRi003-A iPSC lines can be used for the characterization of CAV3-associated pathomechanisms and for developing new therapeutic options.
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Células Madre Pluripotentes Inducidas , Enfermedades Musculares , Células Madre Pluripotentes , Humanos , Femenino , Persona de Mediana Edad , Células Madre Pluripotentes Inducidas/metabolismo , Factor 4 Similar a Kruppel , Enfermedades Musculares/metabolismo , Enfermedades Musculares/patología , Fibroblastos/metabolismo , Mutación , Diferenciación Celular/genéticaRESUMEN
OBJECTIVE: Lip treatment products often incorporate oils and waxes in their formulations, and a desired outcome of their use is to prevent lip dryness and roughness as well as help to repair this condition. The objective of this study was to combine confocal Raman spectroscopy with skin capacitance (corneometry) and transepidermal water loss (closed chamber Aquaflux system) measurements, in the evaluation of the degree of moisturization and lip skin penetration of a fruit wax (Rhus vernicula peel cera) and natural oil-based (Cocos nucifera fruit oil and Olea europea oil) lip care product, following a single application. METHODS: The study was conducted on a total of 15 healthy female volunteers. Instrumental measurements were performed before and 30 min, 2 h and 6 h after a single application of the product. RESULTS: Lip skin barrier function as well as lip hydration were significantly improved and penetration of olive oil was maintained for at least 6 h post product application. The deposition of the three component lipids (berry fruit wax, coconut oil and olive oil) into the stratum corneum after a single application of the lip care product was maintained and data significant for 2-6 h post product application. Lipid deposition was regarded as a positive long-lasting skin care (depot-) effect combined with a profound hydrating effect for about 6 h. CONCLUSION: The tri-method approach taken in this study is deemed relevant and valid for measuring lip hydration offering a complimentary assessment of the barrier function of lip skin and interactive effects of cosmetic ingredients.
OBJECTIFS: Les formulations des produits de soins des lèvres contiennent souvent des huiles et des cires. En outre, la prévention, voire la réparation de la sécheresse et de la rugosité des lèvres font partie des résultats attendus de l'utilisation de ces produits. Cette étude avait pour objectif d'associer une spectroscopie confocale Raman à des mesures de la capacitance de la peau (cornéométrie) et de la perte d'eau transépidermique (système à chambre fermée Aquaflux), dans l'évaluation du niveau d'hydratation et de pénétration cutanées des lèvres d'une cire à base de fruits (cire d'écorce de Vernis du Japon) et d'un produit de soins des lèvres à base d'huiles naturelles (huile de coco et huile d'olive), après une seule application. MÉTHODES: Au total, l'étude a été menée auprès de 15 volontaires en bonne santé de sexe féminin. Des mesures instrumentales ont été réalisées avant, puis 30 minutes, 2 heures et 6 heures après une seule application du produit. RÉSULTATS: Une amélioration significative de la fonction barrière et de l'hydratation de la peau des lèvres a été observée, et la pénétration cutanée de l'huile d'olive est demeurée stable pendant au moins 6 heures après l'application du produit. Le dépôt des trois lipides entrant dans sa composition (la cire de baies, l'huile de coco et l'huile d'olive) dans la couche cornée s'est prolongé pendant 2 à 6 heures après une seule application du produit de soins des lèvres, présentant ainsi un intérêt significatif pour le recueil de données. Les résultats concernant le dépôt lipidique ont décrit un effet positif et durable dans le soin de la peau associé à une hydratation intense pendant environ 6 heures. CONCLUSION: L'approche à trois méthodes adoptée dans le cadre de cette étude pour mesurer l'hydratation des lèvres est jugée pertinente et valable, car elle offre une évaluation complémentaire de la fonction barrière de la peau des lèvres et des effets interactifs des ingrédients entrant dans la composition des cosmétiques.
Asunto(s)
Cosméticos , Labio , Metabolismo de los Lípidos , Plantas/metabolismo , Espectrometría Raman/métodos , Pérdida Insensible de Agua , Adolescente , Adulto , Anciano , Emolientes/administración & dosificación , Femenino , Humanos , Persona de Mediana Edad , Piel/metabolismo , Adulto JovenRESUMEN
Soft tissue sarcomas (STS) are heterogeneous malignant tumors of mesenchymal origin. Due to low incidence and high number of different histological subtypes, their pathogenesis and thus potential targets for their therapy remain barely investigated. Several studies revealed significant higher EPHB4 expression in malignancies such as prostate and colorectal cancer showing survival advantages for these tumor cells. Therefore we studied the expression of EPHB4 in a total of 46 clinical human specimens of different STS and human fibroblasts. EPHB4 mRNA and protein expression were significantly increased in synovial sarcoma. After targeting EPHB4 in fibrosarcoma, synovial sarcoma, liposarcoma and MFH sarcoma cell lines by siRNA or by inhibition of autophosphorylation using the specific EPHB4 kinase inhibitor NVP-BHG712 a decreased proliferation rate/vitality of synovial- and fibrosarcoma cells was observed. Silencing of EPHB4 significantly reduced the transmigration of synovial sarcoma cells towards fibroblasts and endothelial cells. In addition, we assessed the anti-metastatic effect of EPHB4 inhibition in vivo by intraperitoneal administration of the EPHB4 inhibitor in an appropriate sarcoma lung metastasis xenograft model. As result 43% of NVP-BHG712 treated mice (n = 3/7) developed pulmonary metastases whereas all control mice (n = 5) revealed lung metastases. The residual 57% of mice (n = 4/7) showed only small local tumor cell spots. Size measurements of the Vimentin positive area explained significant decrease in lung metastasis formation (p < 0.05) after EPHB4 kinase inhibition. In summary, these data provide first evidence of the importance of EPHB4 in the tumorigenesis of synovial sarcoma and present EPHB4 as a potential target in the therapy of this malignancy.
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Expresión Génica/genética , Receptor EphB4/genética , Receptor EphB4/metabolismo , Sarcoma/genética , Animales , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Proliferación Celular/genética , Células Cultivadas , Células Endoteliales/efectos de los fármacos , Células Endoteliales/metabolismo , Células Endoteliales/patología , Fibroblastos/efectos de los fármacos , Fibroblastos/metabolismo , Fibroblastos/patología , Expresión Génica/efectos de los fármacos , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/patología , Ratones , Ratones Desnudos , Inhibidores de Proteínas Quinasas/farmacología , ARN Mensajero/genética , ARN Interferente Pequeño/genética , Sarcoma/metabolismo , Sarcoma/patologíaRESUMEN
Arthrofibrosis (af) is defined as a fibrosing disease of the synovial membrane, after joint operations, with painful restricted range of motion. The aim of this paper was to describe the histopathological substrate of af, hitherto only defined by clinical criteria. Based on a group of 222 tissue samples, the characteristic changes to af were analyzed. The control group comprised 29 cases with neosynovialis of the indifferent type. Due to cytoplasmic SM-actin positivity and the absence of specific cytoplasmic reactivity in CD 68 representation, af fibroblasts were characterized as myofibroblasts. In confocal laser-scanning microscopy, ß-catenin-positive aggregates were detected in the cytoplasm. Over and above this, unequivocal colocalization of ß-catenin and the tight junction protein ZO-1 became manifest, particularly on the cell membrane and, partly, in the cytoplasm. A threshold value of 20 ß-catenin-positive cells/HPF was determined. This enables the histopathological diagnosis of an af to be made (sensitivity: 0.733, specificity: 0.867). Af is a fibrosing disease of the synovial membrane with variable grade of fibrotization (fibroblast cellularity). A threshold value of 20 ß-catenin-positive fibroblasts per HPF was defined, which enables the histopathological diagnosis of af.
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Inmunohistoquímica , Artropatías/diagnóstico , Microscopía Confocal , Complicaciones Posoperatorias/diagnóstico , Membrana Sinovial/química , Membrana Sinovial/patología , beta Catenina/análisis , Actinas/análisis , Antígenos CD/análisis , Antígenos de Diferenciación Mielomonocítica/análisis , Biomarcadores/análisis , Biopsia , Estudios de Casos y Controles , Fibrosis , Humanos , Artropatías/metabolismo , Artropatías/patología , Miofibroblastos/química , Miofibroblastos/patología , Complicaciones Posoperatorias/metabolismo , Complicaciones Posoperatorias/patología , Valor Predictivo de las Pruebas , Índice de Severidad de la Enfermedad , Proteína de la Zonula Occludens-1/análisisRESUMEN
This article gives a detailed description of a protocol using density gradient centrifugation for the enrichment of neuromelanin granules and synaptosomes from low amounts (≥0.15g) of human substantia nigra pars compacta tissue. This has a great advantage compared to already existing methods as it allows for the first time (i) a combined enrichment of neuromelanin granules and synaptosomes and (ii) just minimal amounts of tissue necessary to enable donor specific analysis. Individual specimens were classified as control or diseased according to clinical evaluation and neuropathological examination. For the enrichment of synaptosomes and neuromelanin granules from the same tissue sample density gradient centrifugations using Percoll® and Iodixanol were performed. The purity of resulting fractions was checked by transmission electron microscopy. We were able to establish a reproducible and easy to handle protocol combining two different density gradient centrifugations: using an Iodixanol gradient neuromelanin granules were enriched and in parallel, from the same sample, a fraction of synaptosomes with high purity using a Percoll® gradient was obtained. Our subfractionation strategy will enable a subsequent in depth proteomic characterization of neurodegenerative processes in the substantia nigra pars compacta in patients with Parkinson's disease and dementia with Lewy bodies compared to appropriate controls. BIOLOGICAL SIGNIFICANCE: Key features of Parkinson's disease are the degeneration of dopaminergic neurons in the substantia nigra pars compacta, an associated loss of the brain pigment neuromelanin and a resulting impairment of the neuronal network. The accumulation of iron binding neuromelanin granules is age- and disease-dependent and disease specific alterations could affect the neuronal iron homeostasis leading to oxidative stress induced cell death. The focus of the described method is the analysis of neuromelanin granules as well as axonal cell-endings of nerve cells (synaptosomes) of individual donors (control and diseased). It is the basis for the identification of disease-relevant changes in the iron homeostasis and the generation of new insight into altered protein compositions or regulations which might lead to disturbed communications between nerve cells resulting in pathogenic processes.
Asunto(s)
Gránulos Citoplasmáticos/química , Melaninas , Proteómica/métodos , Sustancia Negra/química , Sinaptosomas/química , Centrifugación por Gradiente de Densidad/métodos , Gránulos Citoplasmáticos/metabolismo , Femenino , Humanos , Masculino , Enfermedades Neurodegenerativas/metabolismo , Sustancia Negra/metabolismoRESUMEN
Persistent spectral hole burning at 4.5 K has been used to investigate the excitonic energy level structure and the excited state dynamics of the recombinant class-IIa water-soluble chlorophyll-binding protein (WSCP) from cauliflower. The hole-burned spectra are composed of four main features: (i) a narrow zero-phonon hole (ZPH) at the burn wavelength, (ii) a number of vibrational ZPHs, (iii) a broad low-energy hole at ~665 and ~683 nm for chlorophyll b- and chlorophyll a-WSCP, respectively, and (iv) a second satellite hole at ~658 and ~673 nm for chlorophyll b- and chlorophyll a-WSCP, respectively. The doublet of broad satellite holes is assigned to an excitonically coupled chlorophyll dimer. The lower-energy holes at ~665 and ~683 nm for chlorophyll b- and chlorophyll a-WSCP, respectively, represent the lower exciton states. Taking into account the parameters of electron-phonon coupling, the lower exciton state can be assigned as the fluorescence origin. The lower exciton state is populated by two processes: (i) exciton relaxation from the higher exciton state and (ii) vibrational relaxation within the lower exciton state. Assuming identical site energies for the two excitonically coupled chlorophyll molecules, the dipole-dipole interaction energy J is directly determined to be 85 and 100 cm(-1) for chlorophyll b- and chlorophyll a-WSCP, respectively, based on the positions of the satellite holes. The Gaussian low-energy absorption band identified by constant fluence hole burning at 4.5 K has a width of ~150 cm(-1) and peaks at 664.9 and 682.7 nm for chlorophyll b- and chlorophyll a-WSCP, respectively. The action spectrum is broader and blue-shifted compared to the fluorescent lower exciton state. This finding can be explained by a slow protein relaxation between energetically inequivalent conformational substates within the lowest exciton state in agreement with the results of Schmitt et al. (J. Phys. Chem. B2008, 112, 13951).
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Clorofila/química , Complejos de Proteína Captadores de Luz/química , Brassica/metabolismo , Clorofila A , Electrones , Complejos de Proteína Captadores de Luz/genética , Complejos de Proteína Captadores de Luz/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Espectrometría de Fluorescencia , Termodinámica , Vibración , Agua/químicaRESUMEN
This short review paper describes spectroscopic studies on pigment-pigment and pigment-protein interactions of chlorophyll (Chl) a and b bound to the recombinant protein of class IIa water soluble chlorophyll protein (WSCP) from cauliflower. Two Chls form a strongly excitonically coupled open sandwich dimer within the tetrameric protein matrix. In marked contrast to the mode of excitonic coupling of Chl and bacterio-Chl molecules in light harvesting complexes and reaction centers of all photosynthetic organisms, the unique structural pigment array in the Chl dimer of WSCP gives rise to an upper excitonic state with a large oscillator strength. This property opens the way for thorough investigations on exciton relaxation processes in Chl-protein complexes. Lifetime measurements of excited singlet states show that the unusual stability towards photodamage of Chls bound to WSCP, which lack any protective carotenoid molecule, originates from a high diffusion barrier to interaction of molecular dioxygen with Chl triplets. Site selective spectroscopic methods provide a wealth of information on the interactions of the Chls with the protein matrix and on the vibronic structure of the pigments. The presented data and discussions illustrate the great potential of WSCP as a model system for systematic experimental and theoretical studies on the functionalizing of Chls by the protein matrix. It opens the way for further detailed analyses and a deeper understanding of the properties of pigment protein complexes.
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Clorofila/metabolismo , Complejos de Proteína Captadores de Luz/metabolismo , Modelos Biológicos , Plantas/metabolismo , Agua/metabolismo , TemperaturaRESUMEN
The crystal structure of the class IIb water-soluble chlorophyll binding protein (WSCP) from Lepidium virginicum is used to model linear absorption and circular dichroism spectra as well as excited state decay times of class IIa WSCP from cauliflower reconstituted with chlorophyll (Chl) a and Chl b. The close agreement between theory and experiment suggests that both types of WSCP share a common Chl binding motif, where the opening angle between pigment planes in class IIa WSCP should not differ by more than 10 degrees from that in class IIb. The experimentally observed (Schmitt et al. J. Phys. Chem. B 2008, 112, 13951) decrease in excited state lifetime of Chl a homodimers with increasing temperature is fully explained by thermally activated superradiance via the upper exciton state of the dimer. Whereas a temperature-independent intersystem crossing (ISC) rate is inferred for WSCP containing Chl a homodimers, that of WSCP with Chl b homodimers is found to increase above 100 K. Our quantum chemical/electrostatic calculations suggest that a thermally activated ISC via an excited triplet state T4 is responsible for the latter temperature dependence.
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Complejos de Proteína Captadores de Luz/química , Temperatura , Agua/química , Dicroismo Circular , Cristalografía por Rayos X , Lepidium/química , Modelos Moleculares , Teoría Cuántica , Solubilidad , Electricidad EstáticaRESUMEN
The present study describes the fluorescence emission properties of recombinant water-soluble chlorophyll (Chl) protein (WSCP) complexes reconstituted with either Chl a or Chl b alone (Chl a only or Chl b only WSCP, respectively) or mixtures of both pigments at different stoichiometrical ratios. Detailed investigations were performed with time and space correlated ps fluorescence spectroscopy within the temperature range from 10 to 295 K. The following points were found: (a) The emission spectra at room temperature (295 K) are well characterized by bands with a dominating Lorentzian profile broadened due to phonon scattering and peak positions located at 677, 684 and 693 nm in the case of Chl a only WSCP and at 665, 675 and 689 nm for Chl b only WSCP. In addition, all spectra contain minor bands in the longer wavelength region. (b) The emission spectra at 10 K of samples suspended in buffer containing 50% glycerol are dominated by bands peaking at 668 nm for Chl b only WSCP and at 685 nm for Chl a only WSCP and samples reconstituted with mixtures of Chl a and Chl b. (c) At 10 K and in buffer with 50% glycerol the decay kinetics of WSCP samples with Chl a only are dominated by a component with a time constant of 6.2 (+/-0.2) ns at 685 nm while those of WSCP containing mixtures of Chl a and Chl b are characterized by a slightly shorter value of 6.0 (+/-0.2) ns. WSCP containing Chl b only exhibits a distinctly longer value of 7.0 (+/-0.3) ns at an emission wavelength of 668 nm. (d) The decay associated emission spectra at 10 K of all samples exhibit at least 3 decay components with time constants of 80-120 ps, 2-4 ns and 6-7 ns in 50% glycerol. These results are consistently described within the framework of our previously presented model (J. Phys. Chem. B 2007, 111, No. 46, 13325; J. Phys. Chem. B 2007, 111, No. 35, 10487) , for the structural motifs of chlorophyll binding to the tetrameric protein matrix of WSCP. It is shown that formation of strongly coupled open sandwich dimers does not lead to quenching of 1Chl a* or 1Chl b*.
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Brassica/enzimología , Complejos de Proteína Captadores de Luz/química , Agua/química , Complejos de Proteína Captadores de Luz/metabolismo , Proteínas de Plantas , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Solubilidad , Espectrometría de Fluorescencia , Temperatura , Factores de TiempoRESUMEN
Plants contain water-soluble chlorophyll-binding proteins (WSCPs) that function neither as antennas nor as components of light-induced electron transfer of photosynthesis but are likely constituents of regulatory protective pathways in particular under stress conditions. This study presents results on the spectroscopic properties of recombinant WSCP from cauliflower reconstituted with chlorophyll b (Chl b) alone or with mixtures of Chl a and Chl b. Two types of experiments were performed: (a) measurements of stationary absorption spectra at 77 and 298 K and CD spectra at 298 K and (b) monitoring of laser flash-induced transient absorption changes with a resolution of 200 fs in the time domain of up to 100 ps. On the basis of a theoretical analysis outlined by Renger et al. (J. Phys. Chem. B 2007, 111, 10487) the data obtained in part (a) are interpreted within a model where tetrameric WSCP binds predominantly two Chl molecules in the form of an excitonically coupled "open sandwich" dimer with a tilt angle of about 30 degrees between the chlorin planes. The time-resolved measurements on Chl a/Chl b heterodimers are described by two exponential kinetics with time constants of 400 fs and 7 ps. These kinetics are assumed to reflect a heterogeneous population of WSCPs with Chl dimers either in excitonic coupled "open sandwich" or weakly coupled geometric arrays. The 400 fs component is assigned to excited-state relaxations from the upper to the lower excitonic level of the strongly coupled "open sandwich" dimer, while the 7-8 ps component probably indicates excitation energy transfer from 1Chl b* to Chl a in a dimer array with weak coupling due to significantly longer mutual distances between the chlorin rings.
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Brassica/química , Complejos de Proteína Captadores de Luz/química , Pigmentos Biológicos/química , Dicroismo Circular , Cinética , Rayos Láser , Transferencia Lineal de Energía , Proteínas Recombinantes/química , Espectrofotometría UltravioletaRESUMEN
Time-local and time-nonlocal theories are used in combination with optical spectroscopy to characterize the water-soluble chlorophyll binding protein complex (WSCP) from cauliflower. The recombinant cauliflower WSCP complexes reconstituted with either chlorophyll b (Chl b) or Chl a/Chl b mixtures are characterized by absorption spectroscopy at 77 and 298 K and circular dichroism at 298 K. On the basis of the analysis of these spectra and spectra reported for recombinant WSCP reconstituted with Chl a only (Hughes, J. L.; Razeghifard, R.; Logue, M.; Oakley, A.; Wydrzynski, T.; Krausz, E. J. Am. Chem. Soc. U.S.A. 2006, 128, 3649), the "open-sandwich" model proposed for the structure of the pigment dimer is refined. Our calculations show that, for a reasonable description of the data, a reduction of the angle between pigment planes from 60 degrees of the original model to about 30 degrees is required when exciton relaxation-induced lifetime broadening is included in the analysis of optical spectra. The temperature dependence of the absorption spectrum is found to provide a unique test for the two non-Markovian theories of optical spectra. Based on our data and the 1.7 K spectra of Hughes et al. (2006), the time-local partial ordering prescription theory is shown to describe the experimental results over the whole temperature range between 1.7 K and room temperature, whereas the alternative time-nonlocal chronological ordering prescription theory fails at high temperatures. Modified-Redfield theory predicts sub-100 fs exciton relaxation times for the homodimers and a 450 fs time constant in the heterodimers. Whereas the simpler Redfield theory gives a similar time constant for the homodimers, the one for the heterodimers deviates strongly in the two theories. The difference is explained by multivibrational quanta transitions in the protein which are neglected in Redfield theory.
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Clorofila/química , Complejos de Proteína Captadores de Luz/química , Modelos Químicos , Brassica , Clorofila A , Dicroismo Circular , Cinética , Modelos Moleculares , Óptica y Fotónica , Análisis Espectral , Electricidad EstáticaRESUMEN
alpha(2)-adrenergic receptors (alpha(2)AR) couple to multiple effectors including adenylyl cyclase and phospholipase C. We hypothesized that signaling selectivity to these effectors is dynamically directed by kinase-sensitive domains within the third intracellular loop of the receptor. Substitution of Ala for Ser232, which is in the N-terminal region of this loop in the alpha(2A)AR, resulted in a receptor that was markedly uncoupled ( approximately 82% impairment) from stimulation of inositol phosphate accumulation while the capacity to inhibit adenylyl cyclase remained relatively intact. In S232A alpha(2A)AR transfected cell membranes, agonist-promoted [(35)S]GTPgammaS binding was reduced by approximately 50%. Coexpression of modified G proteins rendered insensitive to pertussis toxin revealed that the S232A receptor was uncoupled from both G(i) and G(o). S232 is a potential PKC phosphorylation site, and whole cell phosphorylation studies showed that the mutant had depressed phosphorylation compared to wild type (1.3- vs 2.1-fold/basal). Consistent with S232 directing coupling to phospholipase C, PMA exposure resulted in approximately 67% desensitization of agonist-promoted inositol phosphate accumulation without significantly affecting inhibition of adenylyl cyclase. The dominant effect of mutation or phosphorylation at this site on inositol phosphate as compared to cAMP signaling was found to most likely be due to the low efficiency of signal transduction via phospholipase C vs adenylyl cyclase. Taken together, these results indicate that S232 acts as a selective, PKC-sensitive, modulator of effector coupling of the alpha(2A)AR to inositol phosphate stimulation. This represents one mechanism by which cells route stimuli directed to multifunctional receptors to selected effectors so as to attain finely targeted signaling.
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Proteína Quinasa C/metabolismo , Receptores Adrenérgicos alfa 2/metabolismo , Transducción de Señal , Toxina de Adenilato Ciclasa , Inhibidores de Adenilato Ciclasa , Adenilil Ciclasas/metabolismo , Agonistas alfa-Adrenérgicos/farmacología , Secuencia de Aminoácidos , Animales , Células CHO , Cricetinae , AMP Cíclico/antagonistas & inhibidores , AMP Cíclico/metabolismo , Epinefrina/farmacología , Guanosina 5'-O-(3-Tiotrifosfato)/química , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Hemostáticos/farmacología , Humanos , Fosfatos de Inositol/metabolismo , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Toxina del Pertussis , Fosforilación , Receptores Adrenérgicos alfa 2/química , Receptores Adrenérgicos alfa 2/genética , Alineación de Secuencia , Trombina/farmacología , Factores de Virulencia de Bordetella/farmacologíaRESUMEN
In the present study the function of axonal transport in individual neurons under aluminium intoxication was investigated experimentally in comparison with controls. We used the technique of microinjection of horseradish peroxidase (HRP) in dissociated dorsal root ganglia (DRG) neurons and neurons of explant cultures of DRG. Different exposure periods (1 and 6 hours as well as 6 and 10 days) to aluminium were analysed quantitatively. This analysis revealed an impaired anterograde transport of HRP already after a short aluminium intoxication period of only 1 hour in DRG cells in vitro, an effect that increased with a prolonged aluminium exposure for up to 10 days. Hence, functional alterations of the anterograde transport caused by aluminium could be detected even after short exposure periods. Furthermore, the effects of aluminium on anterograde transport mechanisms were reversible 8 days after removal of aluminium. To determine how aluminium affects the cytoskeleton, we performed immunohistochemistry and electron microscopy on cultured DRG neurons. Distinct morphological alterations of the cytoskeleton, especially the accumulation of phosphorylated neurofilaments, appeared after 6 days of aluminium exposure. Our results suggest that neurofilaments are indispensable to the functional integrity of the cytoskeleton and its ability to mediate microtubule-based axonal transport processes.
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Aluminio/farmacología , Transporte Axonal/efectos de los fármacos , Ganglios Espinales/citología , Neuronas/efectos de los fármacos , Animales , Axones/fisiología , Axones/ultraestructura , Células Cultivadas , Embrión de Pollo , Peroxidasa de Rábano Silvestre/farmacocinética , Técnicas In Vitro , Microinyecciones , Microscopía Electrónica , Neuronas/fisiología , Neuronas/ultraestructuraRESUMEN
The distribution of the neurotrophins BDNF and NT-3 as well as their corresponding high-affinity receptors trkB and trkC was characterized by immunohistochemistry in the developing retino-tectal system of the pigeon. These neurotrophins are known to be important for survival and development of neuronal tissues, but also for activity-dependent neuronal plasticity. In pigeons visual asymmetry is established at the morphological and behavioral level due to a natural asymmetrical light input before hatch, which is followed by a posthatch period of consolidation with unbiased light stimulation. Since the retino-tectal system is the crucial entity of these events, we studied the retinal and the tectal distribution of these neurotrophins and their receptors during retino-tectal formation, to analyze the developmental sequences to which these neurotrophins are tuned. Here we demonstrate that in altricial pigeons no retinal immunolabeling of BDNF, NT-3 or their receptors could be detected before hatch, although a prominent tectal labeling pattern throughout most layers was evident. After hatch, both neurotrophins and their receptors showed a dramatic increase of retinal and tectal distribution. While the tectal and retinal protein synthesis of NT-3 vanished after 2 weeks, that of BDNF could still be revealed in adults. Therefore, the establishment of the retino-tectal system does not seem to depend on these neurotrophins before hatch, although they are probably utilized to shape the intratectal wiring pattern. In contrast, BDNF and NT-3 could play a prominent role in posthatch retino-tectal plasticity, as the consolidation of tectal asymmetries requires posthatch modifications of tectal circuits and proceeds within the first two posthatching weeks. These data are comparable with the distribution of neurotrophins in the retino-tectal system of chicks, although the onset of neurotrophin synthesis seems to be earlier in precocial chicks.
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Factor Neurotrófico Derivado del Encéfalo/metabolismo , Columbidae/metabolismo , Neurotrofina 3/metabolismo , Receptor trkB/metabolismo , Receptor trkC/metabolismo , Retina/metabolismo , Colículos Superiores/metabolismo , Animales , Factor Neurotrófico Derivado del Encéfalo/análisis , Columbidae/embriología , Columbidae/crecimiento & desarrollo , Embrión no Mamífero/metabolismo , Inmunohistoquímica , Neurotrofina 3/análisis , Receptor trkB/análisis , Receptor trkC/análisis , Retina/embriología , Retina/crecimiento & desarrollo , Colículos Superiores/embriología , Colículos Superiores/crecimiento & desarrolloRESUMEN
Epac1 is a Rap-specific guanine-nucleotide exchange factor (GEF) which is activated by the binding of cAMP to a cyclic nucleotide monophosphate (cNMP)-binding domain. We investigated the equilibrium and dynamics of the interaction of cAMP and Epac1 using a newly designed fluorescence analogue of cAMP, 8-MABA-cAMP. We observed that the interaction of cAMP, measured by competition with 8-MABA-cAMP, with an isolated cNMP binding domain of Epac1 has an overall equilibrium constant (Kd) of 4 microM and that the kinetics of the interaction are highly dynamic. The binding properties of cAMP are apparently not affected when the catalytic domain is present, despite the fact that binding of cAMP results in activation of Epac1. This indicates that for the activation process, no appreciable binding energy is required. However, when bound to Rap1b, the apparent Kd of Epac to cAMP was about fivefold lower, suggesting that substrate interaction stabilizes cAMP binding. Since the fluorescent analogues used here were either less able or unable to induce activation of Epac1, we concluded that the binding of nucleotide to Epac and the activation of GEF activity are uncoupled processes and that thus appropriate cAMP analogues can be used as inhibitors of the Epac1-mediated signal transduction pathway of Rap.
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AMP Cíclico/metabolismo , Factores de Intercambio de Guanina Nucleótido/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Sitios de Unión , AMP Cíclico/análogos & derivados , Fluorescencia , Productos del Gen vpr/metabolismo , Vectores Genéticos , Glutatión Transferasa/metabolismo , Marcaje Isotópico , Cinética , Estructura MolecularRESUMEN
The alpha(2A)-adrenergic receptor (AR) undergoes rapid agonist-promoted desensitization due to phosphorylation by G protein-coupled receptor kinases (GRKs) 2 and 3 at serines in the third intracellular loop of the receptor. In contrast, the alpha(2C)AR fails to display such desensitization or phosphorylation, which has been presumed to be due to this receptor lacking GRK phosphorylation sites. However, the alpha(2C)AR has multiple serines and threonines in putative favorable motifs within its third intracellular loop. We considered that the conformation of the third intracellular loop imposed by agonists binding to the transmembrane-spanning domains could be the basis of this subtype-specific property, rather than the presence or absence of phosphoacceptors per se. To address this, alpha(2A)/alpha(2C) third loop chimeric receptors were constructed. In whole cell phosphorylation studies, the alpha(2A) with the alpha(2C) third loop receptor underwent agonist-promoted phosphorylation while the alpha(2C) with the alpha(2A) third loop receptor did not, indicating that the agonist interaction with the parent receptor backbone establishes the phosphorylation phenotype. We postulated then that agonists with diverse structures that distinctly interact with alpha(2)AR should display different degrees of phosphorylation independent of receptor activation. Indeed, several full and partial agonists were identified, which evoked phosphorylation that was not related to intrinsic activity as established by [(35)S]guanosine 5'-3-O-(thio)triphosphate binding. Taken together, it appears that phosphorylation of the alpha(2)AR evoked by agonist is highly sensitive to the conformation of the third intracellular loop induced/stabilized by agonist to such an extent that these properties dictate the extent of phosphorylation of the loop when phosphoacceptors are present, and are the basis for subtype-specific phosphorylation.
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Agonistas alfa-Adrenérgicos/metabolismo , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Receptores Adrenérgicos alfa 2/metabolismo , Proteínas Recombinantes de Fusión/metabolismo , Secuencia de Aminoácidos , Animales , Células COS , Cricetinae , Cricetulus , Quinasa 3 del Receptor Acoplado a Proteína-G , Datos de Secuencia Molecular , Conformación Proteica , Receptores Adrenérgicos alfa 2/química , Receptores Adrenérgicos alfa 2/clasificación , Quinasas de Receptores Adrenérgicos betaRESUMEN
We have investigated the effects of taxol on the axonal transport of horseradish peroxidase (HRP) in dorsal root ganglia (DRG) cells and their neuronal cytoskeleton. The former were analysed by microinjection of HRP into single DRG cells and the latter was studied by means of immunohistochemistry and cryo-electron microscopy. In cultured and untreated DRG cells, microinjected HRP was typically transported anterogradely several hundred micrometres along their neurites. Different exposure periods (1, 2 and 3 days) to taxol were analysed. The axonal transport of HRP in DRG cells was time-dependently impeded by taxol. After the drug had been washed out, a recovery of the axonal transport of HRP was observed and confirmed by quantitative analysis. Cryo-electron microscopy revealed an abnormal aggregation of axonal and cytoplasmic microtubules, associated with a decreased amount of cross-linking structures, in taxol-treated DRG cell cultures. After 3 days of taxol exposure, microtubule-associated proteins and Tau-protein were restricted to the cellular somata but the neurofilament network and tubulin-proteins seemed to be unaffected. Our results demonstrate, for the first time, an inhibition of anterograde axonal transport of HRP in single neurons by taxol. This effect is reversible and seems not to be caused by cellular damage, but is rather a consequence of an altered organisation of microtubules and/or microtubule-associated proteins.
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Transporte Axonal/efectos de los fármacos , Ganglios Espinales/citología , Peroxidasa de Rábano Silvestre/metabolismo , Microtúbulos/efectos de los fármacos , Proteínas del Tejido Nervioso/efectos de los fármacos , Neuronas Aferentes/efectos de los fármacos , Paclitaxel/farmacología , Animales , Células Cultivadas , Embrión de Pollo , Microscopía por Crioelectrón , Citoesqueleto/efectos de los fármacos , Citoesqueleto/ultraestructura , Microinyecciones , Microtúbulos/fisiología , Microtúbulos/ultraestructura , Proteínas del Tejido Nervioso/fisiología , Proteínas del Tejido Nervioso/ultraestructura , Neuronas Aferentes/metabolismo , Neuronas Aferentes/ultraestructuraRESUMEN
We have investigated the potential for protein kinase C (PKC) to phosphorylate and desensitize the alpha2A-adrenergic receptor (alpha2AAR). In whole-cell phosphorylation studies, recombinantly expressed human alpha2AAR displayed an increase in phosphorylation after short-term exposure to 100 nM phorbol 12-myristate-13-acetate (PMA) that was blocked by preincubation with a PKC inhibitor. This increase in receptor phosphorylation over basal amounted to 172 +/- 40% in COS-7 cells and 201 +/- 40% in Chinese hamster ovary cells. In permanently transfected Chinese hamster fibroblast cells, PKC activation by brief exposure of the cells to PMA resulted in a marked desensitization of alpha2AAR function, amounting to a 68 +/- 4% decrease in the maximal agonist (UK14304)-stimulated intracellular calcium release. Such desensitization was blocked by the PKC inhibitor bisindolylmaleimide I and was not evoked by an inactive phorbol ester. The desensitization of this agonist response was not caused by PKC-mediated augmentation of G protein-coupled receptor kinase activity, because PMA-promoted desensitization of a mutated alpha2AAR that lacked G protein-coupled receptor kinase phosphorylation sites was identical to that of wild-type alpha2AAR. To test whether PKC phosphorylation is a mechanism by which alpha2AAR can be regulated by other receptors, the alpha1bAR was co-expressed with the alpha2AAR in Chinese hamster ovary cells. Upon selective activation of alpha1bAR, the function of alpha2AAR underwent a 53 +/- 5% desensitization. Thus, cellular events that result in PKC activation promote phosphorylation of the alpha2AAR and lead to substantial desensitization of receptor function. This heterologous regulation also represents a mechanism by which rapid crosstalk between the alpha2AAR and other receptors can occur.
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Proteína Quinasa C/metabolismo , Receptores Adrenérgicos alfa 2/metabolismo , Animales , Células CHO/metabolismo , Células COS/metabolismo , Calcio/metabolismo , Cricetinae , Humanos , Fosforilación , Receptores Adrenérgicos alfa 2/genética , Transducción de Señal , TransfecciónRESUMEN
The tectofugal system of the pigeon was examined for the distribution of several glutamate-receptor subunits (AMPA Glu R1, Glu R2/3, Glu R4) and the calcium binding protein parvalbumin. With respect to the different antigens, a heterogeneous distribution was observed. Within the optic tectum, the Glu R1 like immunoreactivity was limited to the layers 2-5, 9, 10, and sparsely in layer 13, whereas the antibody to Glu R2/3 stained cell bodies in layers 9, 10, and very heavily in layer 13. In the rotundus only the Glu R4 antigen was expressed, while within the ectostriatal complex a large number of Glu R2/3 and a smaller contingent of Glu R4 positive neurons were stained. Quantitative analysis proved significant heterogeneities of these antigens in the mesencephalic as well as the diencephalic centre of the tectofugal pathway. The number of Glu R2/3 positive neurons undergoes a two-fold increase from the dorsal to the ventral lamina 13 of the optic tectum. Alterations in the amount of immunoreactive neurons were also observed within the rotundus, since the number of Glu R4 positive cells decreased from dorsal to ventral. Morphological differences and their correlation with functional specializations in visual information processing are discussed.
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Neuronas/citología , Nervio Óptico/citología , Receptores AMPA/análisis , Colículos Superiores/citología , Vías Visuales/citología , Animales , Columbidae , Técnica del Anticuerpo Fluorescente Indirecta , Inmunohistoquímica , Nervio Óptico/anatomía & histología , Colículos Superiores/anatomía & histología , Vías Visuales/anatomía & histologíaRESUMEN
In order to elucidate the components of the oxygen sensory complex in HepG2 cells which regulates the production of erythropoietin, we have microinjected recombinant variants of the human small GTP-binding protein hRac1 and measured their effects on the production of reactive oxygen species (ROS) by the dihydrorhodamine-123 technique. The dominant-negative mutant hRac1(T17N) inhibits the NADH-stimulated production of ROS in HepG2 cells, whereas the constitutively activated hRac1(G12V) leads to an increase in intracellular ROS concentration. Reverse transcriptase PCR analysis showed that the hRac1, but not the hRac2, gene is expressed in HepG2 cells. These results demonstrate that hRac1, and not hRac2, is involved in the regulation of ROS production in HepG2 cells and suggest that hRac1 specifically functions in the non-phagocytic NAD(P)H oxidase complex.