RESUMEN
Trichoderma harzianum strains T4 and T6, Acrophialophora nainiana, and Humicola grisea var. thermoidea were screened for their ability to produce carbohydrate-degrading enzyme activities in a medium containing banana plant residue as the carbon source. The best balance of enzyme activities was obtained from cultures of H. grisea var. thermoidea. Xylanase activity from crude extract of A. nainiana had a maximum activity at pH 5.5-7.0 and a temperature range of 50-55 degrees C. It was stable up to 55 degrees C at pH 7.0 for at least 2 h. The fungi were also able to produce xylanase and pectinase activities when grown on extractives as substrate.
Asunto(s)
Frutas/metabolismo , Glicósido Hidrolasas/biosíntesis , Hongos Mitospóricos/enzimología , Medios de Cultivo , Concentración de Iones de Hidrógeno , Especificidad por Sustrato , TemperaturaRESUMEN
We describe the cytogenetic study of two basal cell carcinomas. Only single chromosomally abnormal clones could be detected in both. In addition, many nonclonal changes were seen in the samples, which may represent small neoplastic clones or the result of a basic molecular defect induced by carcinogens.
Asunto(s)
Carcinoma Basocelular/genética , Aberraciones Cromosómicas/genética , Neoplasias Cutáneas/genética , Anciano , Femenino , Humanos , Cariotipificación , Masculino , Persona de Mediana EdadRESUMEN
The present report describes the karyotypic findings in cells from a Wilms' tumor. The most consistent cytogenetic abnormalities detected consisted of translocations involving break and fusion of chromosomal telomeres and telomeric associations frequently affecting the terminus of the short arms of chromosomes 14 and 17.
Asunto(s)
Aberraciones Cromosómicas , Neoplasias Renales/genética , Telómero/ultraestructura , Translocación Genética , Tumor de Wilms/genética , Preescolar , Bandeo Cromosómico , Cromosomas Humanos Par 14 , Cromosomas Humanos Par 17 , Humanos , Cariotipificación , Neoplasias Renales/patología , Masculino , Tumor de Wilms/patologíaRESUMEN
Chromosome analysis of short-term cultures from a basal cell carcinoma was performed. The analyzed karyotypes showed a pseudodiploid clone characterized by a der(4)t(4;14) (p14;p11) and a concomitant inversion of the same chromosome 4 involved in the t(4;14) with the breakpoints at p14 and q25.
Asunto(s)
Carcinoma Basocelular/genética , Aberraciones Cromosómicas , Cromosomas Humanos Par 14 , Cromosomas Humanos Par 4 , Neoplasias Nasales/genética , Inversión Cromosómica , Femenino , Humanos , Cariotipificación , Persona de Mediana Edad , Translocación Genética , Células Tumorales CultivadasRESUMEN
Short-term cultures of a collagenase disaggregated multinodular goiter was shown by cytogenetic analysis to have the mosaic karyotype 47,XX, +7/48,XX, +7, +17/49,XX, +7, +10, +17. No cytogenetic data on goiter are available for comparison with the present case.
Asunto(s)
Bocio/genética , Mosaicismo , Nódulo Tiroideo/genética , Adulto , Células Cultivadas , Femenino , Bocio/patología , Bocio/cirugía , Humanos , Cariotipificación , Nódulo Tiroideo/patología , Nódulo Tiroideo/cirugía , TiroidectomíaRESUMEN
Chromosome analysis of short-term culture of a basal cell carcinoma showed five clonal chromosome abnormalities, t(9;14)(q12 or q13;p11), del(1)(q23 or q25), trisomy 5, trisomy 7, and monosomy X. In addition, several nonclonal structural and numerical changes were seen in the tumor cells.
Asunto(s)
Carcinoma Basocelular/patología , Neoplasias Cutáneas/patología , Anciano , Carcinoma Basocelular/genética , Aberraciones Cromosómicas/patología , Trastornos de los Cromosomas , Células Clonales , Femenino , Humanos , Cariotipificación , Neoplasias Cutáneas/genéticaRESUMEN
Bloom's syndrome (BS) is an autosomal recessive disease characterized by short stature, sensitivity to sunlight, and telangiectasic malar erythema. It is associated to chromosomal breakage, to primary combined immunodeficiency, and to a high incidence of neoplasias. The authors report the case of two siblings with BS and associated immunodeficiency. Both patients were male and 5 (A) and 4 (B) years old at the time of diagnosis. Chronic diarrhea, recurrent otitis media, purulent rhinitis, conjunctivitis and pyodermatitis were reported by patient A. Patient B was admitted with diagnosis of bilateral neuroblastoma and had the tumor resected. Later on, he presented with oral moniliasis, herpetic stomatitis, and skin abscesses. This patient did not have recurrent infections. Immunological evaluation showed normal serum levels of CH50, C3, and C4 for both patients. Serum IgG, IgA, IgM, and salivary IgA levels were: 455 mg/dl, 15mg/dl, 20mg/dl, 0.6mg/dl for A, and 400mg/dl, 15mg/dl, 20mg/dl, and 0.2mg/dl for B, respectively. Serum antipolio antibodies (1, 2, and 3) were normal, and low levels of isohemagglutinins were observed in both patients. T cells subset determination showed: patient A--OKT3 = 66%, OKT4 = 33%, OKT8 = 32%, and 4/8 ratio = 1.0; patient B--OKT3 = 70%, OKT4 = 32%, OKT8 = 34%, and 4/8 ratio = 1.0. In vitro cellular immune response to PHA was depressed only in patient B. Patients karyotype showed chromosomal breaks with sister chromatid exchanges. Neither patient had abnormal alphafetoprotein and carcinoembryonic antigen serum levels. The rarity of such associations justifies the presentation of the cases.