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Exp Anim ; 63(2): 169-73, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24770642

RESUMEN

Prevalence of Helicobacter is mostly unknown in laboratory animals in Thailand. The 221 mice feces/cecum from 8 universities, 2 pharmaceutical companies and 3 research institutions in Thailand were surveyed for the prevalence and distribution of Helicobacter species by using the Electrochemical DNA chip. Helicobacter were detected 23/46 samples in Specific Pathogen Free (SPF) and 168/175 in conventional condition. Prevalence of Helicobacter were 98%, 96%, 92% and 78% in South (n=40), Northeast (n=40), North (n=25) and Central area (n=116), respectively. Only Central area holds SPF facility resulting in Helicobacter prevalence that seems to be lower than other areas. Three species of Helicobacter were detected in feces/cecum samples by sequence analysis: H. rodentium (67.0%, 148 samples), Helicobacter sp. MIT 01-6451 (15.4%, 34 samples), and unidentified Helicobacter species (14.1%, 9 samples). The results suggested that H. rodentium is the most common species of Helicobacter in laboratory mice in Thailand.


Asunto(s)
Animales de Laboratorio/microbiología , Helicobacter/aislamiento & purificación , Laboratorios/estadística & datos numéricos , Animales , Ciego/microbiología , Heces/microbiología , Helicobacter/patogenicidad , Ratones/microbiología , Análisis de Secuencia por Matrices de Oligonucleótidos , Prevalencia , Organismos Libres de Patógenos Específicos , Tailandia/epidemiología
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