Edible mushrooms trending in food: Nutrigenomics, bibliometric, from bench to valuable applications.

The worldwide consumption, health-promoting and nutritional properties of mushrooms have been extensively researched over a decade. Although, wide range of edible mushrooms is still unexplored, which can be a valuable source of bioactive compounds in dietary supplements and biopharma industry. Mushrooms represent as dynamic source of nutrients lacking in food from plant or animal origin thus, considered as vital functional food utilized for prevention of numerous diseases. The unique bioactive compounds in mushroom and their anti-inflammatory, anti-tumour and other health attributes have been discussed. The preventive action of mushroom on maintaining the gut health and their property to act as pro, pre or symbiotic is also elucidated. The direct prebiotic activity of mushroom affects gut haemostasis and enhances the gut microbiota. Recent reports on role in improving the brain health and neurological impact by mushroom are mentioned. The role of bioactive components in mushroom with relation to nutrigenomics have been explored. The nutrigenomics has become a crucial tool to assess individuals' diet according its genetic make-up and thus, cure of several diseases. Undeniably, mushroom in present time is regarded as next-generation wonder food, playing crucial role in sustaining health, thus, an active ingredient of food and nutraceutical industries.

Unveiling heavy metal pollution dynamics in sediments of river Ulhas, Maharashtra, India: a comprehensive analysis of anthropogenic influence, pollution indices, and health risk assessment.

Metals and metalloids tainting sediments is an eminent issue, predominantly in megacities like Mumbai and Navi Mumbai, requiring an exhaustive examination to identify metal levels in river bodies that serve various populations. Thus, utilising pollution indices, multivariate analysis, and health risk assessment studies, we propose a novel investigation to examine the metal content in the Ulhas River sediments, a prominent agricultural and drinking water supply (320 million-litre per day) near Mumbai in Maharashtra, India. The eleven metals and metalloids (As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn) were examined monthly from 10 stations totaling 120 sediment specimens from October 2022 to September 2023. Investigations revealed that average values of Cr, Cu, Hg, and Ni exceeded Australian and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council values, while all metals exceeded World surface rock average limits except As. Various pollution indices showed that upstream sites had none to low level contamination, whereas downstream locations had moderate to considerable contamination, suggesting anthropogenic influences. Furthermore, multivariate analysis including correlation, cluster, and principal component analysis identified that sediment pollution was mostly caused by anthropogenic activities. Lastly, health risk assessment indicated Fe was non-carcinogenic to children, whereas Cr and Ni were carcinogenic to children and adults, with children being more susceptible. Thus, from the findings of the study it is clear that, despite low to moderate pollution levels, metals may have significant repercussions, thus requiring long-term planning, frequent monitoring, and metal abatement strategies to mitigate river contamination.

Retrospective Study of Anesthesia-Related Complications in Elderly Patients Undergoing Surgery.

Background: Anesthesia administration in elderly surgical patients presents unique challenges due to age-related physiological changes and comorbidities. Knowing anesthesia-related complications is crucial for optimizing perioperative care in this vulnerable population. Methods: A retrospective review of electronic medical records was conducted to identify anesthesia-related complications in elderly subjects aged 65 and above undergoing surgery between January 2018 and December 2020. Data collection included demographic characteristics, comorbidities, anesthesia techniques, and postoperative outcomes. Results: There were a total of 100 elderly patients. Cardiovascular events (35%) were the most common complication, followed by respiratory issues (20%) and adverse drug reactions (15%). Statistical analysis revealed significant associations between cardiovascular events, respiratory issues, adverse drug reactions, and anesthesia. Conclusion: Anesthesia-related complications pose significant risks in elderly surgical patients. Tailored anesthesia management strategies, including comprehensive preoperative assessment and vigilant intraoperative monitoring, are essential for mitigating perioperative risks and improving outcomes in this vulnerable population.

Analgesic Effect of Caudal and Abdominal Nerve Blocks among Children Undergoing Inguinal Surgeries: An Original Research.

Context: Pediatric inguinal surgeries sometimes cause considerable post-operative pain that requires effective analgesics. Caudal and abdominal nerve blocks may help this population with pain. It is uncertain how successful they are compared. Methods: A randomized controlled trial included 70 pediatric inguinal surgery patients. An abdominal (ANB) or caudal nerve block (CNB) was randomly assigned to participants in addition to usual analgesic treatment. Post-operative pain was measured at various times using a standardized scale. The initial rescue analgesia time and dose were recorded. Statistics were used to compare CNB and ANB results. Results: The CNB and ANB groups were demographically similar. At every time point, the two groups' analgesic usage and post-operative pain were similar. No serious adverse events occurred in either group. Conclusion: CNB and ANB provide equivalent analgesia for pediatric inguinal surgeries. Both approaches alleviate pain well and have similar post-operative effects. Individual nerve block approaches must be chosen based on patient features and clinical considerations. More research is needed to determine each procedure's long-term safety and results.

Retrospective Analysis of the Effectiveness of Laser Therapy in the Treatment of Acne Scarring.

Objective: This retrospective analysis aimed to estimate the efficiency of laser therapy in the management of acne scarring, assessing changes in scar severity, patient satisfaction, and adverse events. Methods: A cohort of 150 subjects with clinically diagnosed acne scarring who underwent laser therapy. Data were extracted from electronic medical records, and baseline characteristics, scar types, and management parameters were analyzed. Outcome measures included changes in scar severity scores, patient-reported satisfaction, and documentation of adverse events. Statistical analyses were conducted using appropriate methods, with sub-group analyses based on scar type and laser modality. Results: Following laser therapy, a statistically significant decrease in scar severity scores was seen across all scar types (P < 0.05). Patient satisfaction was high, with 75% reporting significant improvement. Adverse events were mild and transient, including erythema and post-inflammatory hyperpigmentation. Sub-group analyses did not reveal significant differences in management outcomes based on scar type or laser modality. Conclusion: Laser therapy demonstrates effectiveness in reducing acne scarring with a favorable safety profile. The research supports its role as a versatile management modality for diverse scar presentations, emphasizing the need for further research to optimize protocols and explore combination therapies.

Retrospective Review of Tonsillectomy and Adenoidectomy Outcomes in Pediatric Patients: Complications and Recurrence Rates.

Objective: This retrospective review aimed to analyze the outcomes of tonsillectomy and adenoidectomy (T and A) procedures in pediatric patients, focusing on complications and recurrence rates. Methods: Medical records of pediatric patients who underwent T and A were retrospectively reviewed. Data on patient demographics, preoperative indications, surgical techniques, intraoperative findings, postoperative complications, and recurrence rates were collected and analyzed. Results: A total of 240 pediatric patients were included in the study. Postoperative complications occurred with postoperative bleeding being the most common (5.2%). Other complications included infection (3.1%), respiratory compromise (1.7%), and velopharyngeal insufficiency (0.6%). Recurrence of tonsillitis and adenoiditis was observed in 6.7% of cases, respectively. Conclusion: This retrospective review provides valuable insights into the outcomes of T and A procedures in pediatric patients. Postoperative complications, including bleeding and infection, underscore the importance of meticulous surgical technique and postoperative care. Recurrence rates of tonsillitis and adenoiditis highlight the need for long-term follow-up and surveillance in pediatric patients undergoing T and A.

Comparative Analysis of Surgical Techniques for Wisdom Tooth Extraction.

Background: Wisdom tooth extraction often requires various surgical techniques due to differences in tooth position, root morphology, and patient characteristics. This research aims to compare traditional surgical extraction with minimally invasive techniques such as piezo surgery and laser-assisted extraction, as well as extraction with the aid of 3D imaging and navigation systems. Methods: Patients requiring wisdom tooth extraction were randomly assigned to one of the surgical technique groups. Preoperative imaging assessed tooth position and root morphology. Intraoperative variables and postoperative outcomes were recorded, including pain levels, swelling, and healing time. Patient-reported outcomes were assessed using standardized questionnaires. Results: Piezo surgery, 3D imaging, and navigation-guided extraction techniques demonstrated a shorter mean duration of surgery and lower incidence of intraoperative complications compared to traditional extraction and laser-assisted extraction. Postoperative outcomes were superior in the 3D imaging and navigation-guided extraction group, with lower pain scores, reduced swelling, and faster healing time. Conclusion: Advanced imaging-guided techniques, particularly 3D imaging and navigation-guided extraction, offer significant benefits for patients undergoing wisdom tooth extraction by improving surgical precision, minimizing complications, and enhancing postoperative outcomes. Incorporating these technologies into routine practice can optimize patient care and outcomes in oral surgery.

Analysis of Pregnancy Outcomes in Women with Polycystic Ovary Syndrome (PCOS): A Retrospective Study.

Introduction: Although PCOS affects reproductive health, its consequences on the outcome of pregnancies are still up for discussion. Comparing the pregnancy outcomes of women with PCOS to a control group was the goal of this retrospective research. Methods: Analysis of data from tertiary care centers between 2017 and 2022 was done in retrospective. While the controls (n = 300) matched for age, BMI, and parity, the PCOS group (n = 300) satisfied Rotterdam criteria. Maternal-fetal health, birth outcomes, and gestational problems were evaluated. Results: Compared to controls, women with PCOS had a greater incidence of preeclampsia (12.3% vs. 8.1%, P = 0.023) and gestational diabetes (18.7% vs. 9.8%, P < 0.001). On the other hand, there were no notable variations in low birth weight (P = 0.589) or preterm delivery (P = 0.321). Conclusion: In summary, the correlation between PCOS and increased risks of gestational diabetes and preeclampsia emphasizes the necessity of customized therapies. The intricacy of PCOS's influence on birth outcomes is shown by the inconclusive results regarding preterm delivery and low birth weight, which call for more research to enhance mother and newborn health in this group.

A Study on Materials Used and Longevity of Implant-Supported Prostheses.

Objective: This retrospective research was directed to investigate the longevity and maintenance requirements of implant-supported prostheses in subjects treated at a tertiary care center. Methods: Patient records of individuals who received implant-supported prostheses between January 2018 and December 2020 were retrospectively analyzed. Demographic information, implant type, prosthetic design, and follow-up data were collected. The primary outcomes assessed were prosthetic survival rates and the incidence of complications requiring maintenance interventions. Statistical analysis was performed to identify associations between demographic and clinical variables and the observed outcomes. Results: The research included 150 subjects with an average follow-up duration of 3 years. The overall survival rate of implant-supported prostheses was 90%, with 20% requiring maintenance interventions. Common complications included peri-implantitis (10%), prosthetic fracture (6%), and screw loosening (4%). Significant associations were found between smoking status and peri-implantitis (P < 0.05) and between prosthetic design and prosthetic fracture (P < 0.01). Conclusion: Implant-supported prostheses demonstrated a high overall longevity rate, with a subset of subjects requiring maintenance interventions due to complications. Peri-implantitis, prosthetic fracture, and screw loosening were the most prevalent complications, emphasizing the importance of tailored patient care and risk stratification.

Bridging the Healthcare Skill Gap: A Higher Education Perspective: A Data Base Research.

Purpose: There is a rising demand for highly qualified workers in today's ever-changing healthcare job environment. Recognizing higher education's critical role in closing the healthcare skill gap is critical for the long-term growth and prosperity of individuals, healthcare organizations, and the larger economy. Design/Methodology: Within the healthcare sector, education emerges as a pivotal mechanism to address the prevailing skill gap. Universities and colleges offer a wide range of academic programs meticulously designed to cultivate critical thinking, problem-solving capabilities, effective communication, and collaborative teamwork among students. Furthermore, higher education institutions actively collaborate with healthcare employers to tailor specialized programs that precisely align with the requirements of various healthcare roles. Originality/Value: This paper delves into the multi-faceted ways in which universities and colleges can significantly contribute to mitigating the healthcare skills gap. The author emphasizes the critical role played by education in bridging this gap, ensuring that the healthcare workforce is suitably equipped to navigate the swiftly changing job market. Future Scope: By promoting partnerships with industry stakeholders, developing pertinent academic curricula, and offering continuous professional development opportunities, educational institutions empower individuals and healthcare organizations to excel within the dynamic landscape of healthcare delivery.

Comparative Analysis of Braces and Aligners: Long-Term Orthodontic Outcomes.

Objectives: The purpose of this retrospective study was to evaluate the long-term results of orthodontic treatment with traditional braces and Invisalign, with a particular emphasis on treatment length, patient satisfaction, correction of malocclusion, and long-term stability. Methods: Between 2020 and 2022, individuals who had orthodontic treatment at a tertiary care center had their data analysed. Patients with mild to severe malocclusions treated with conventional braces or Invisalign between the ages of 12 and 18 met the inclusion criteria. Assessments were done on results, length of treatment, degree of malocclusion, and long-term stability. With significance set at P < 0.05, statistical analyses comprised t-tests for treatment duration and Chi-square testing for malocclusion correction. Findings: The mean treatment time for Invisalign was much shorter (18 months) than for conventional braces (24 months) (P < 0.001). With 88-90% success rates, both techniques demonstrated remarkable success rates in malocclusion treatment. Even though Invisalign was associated with a somewhat greater percentage of relapse instances, the difference was not statistically significant (P > 0.05). Conclusion: In conclusion, Invisalign showed a much shorter treatment period than conventional braces, yet both showed excellent malocclusion correction. The choice of modality should be based on patient satisfaction, treatment objectives, and case complexity, taking into account the trade-offs between treatment length and potential variations in long-term stability.

Whether Present Era Demand Change in Pharmaceutical Promotional Ways to be More Eco- and Doctor-Friendly? An Observational Study.

Aim: Pharmaceutical promotion is the principal aspect of the healthcare system. In this study, we aimed to portray the opinion of doctors and medical representatives (MRs) on conventional pharmaceutical ways (usage of promotional or educational paper materials and physician drug samples) for pharmaceutical promotion. Materials and Methods: In this cross-sectional observational study, data were collected from doctors and MRs across India using self-administered Google forms. Data were analyzed, and results were drawn. Results: A total of 314 doctors and 272 MRs participated in the study. As per 95.5% of doctors, continuing medical education (CME)/books/online information is the most common and convenient method to update medical knowledge, whereas 67.9% of MRs also think the same. Only 5.5% of doctors prefer paper material provided by pharmaceutical companies to update their knowledge. Most doctors say paper materials provided by pharmaceutical companies contribute less than 25% to product information, rather CME, books, and online information contribute significantly. MRs also think similarly. 66.2% of MRs agree that more than 25% of paper material gets wasted due to non-distribution. 73.2% of doctors and 75.4% of MRs agree that the use of paper materials for product promotion is not cost-effective, even if it contributes to deforestation. Only 51% of doctors use more than 50% of medical samples in patient care and only half of doctors and MRs think expired medical samples are disposed of correctly. 56.1% of doctors and 71.4% of MRs think a significant amount of medical samples are wasted and are hazardous to the environment. Conclusions: Both doctors and MRs are of the opinion that the conventional method of paper promotion, that is, paper material and drug samples, is not cost-effective and also not eco-friendly. Hence, need to rethink - is there a need to change with time?

Assessment of Accuracy of Implant Placement Using Surgical Stent Versus Conventional Technique.

Purpose: The aim of this study was to assess the accuracy of the implant placement using surgical stents versus conventional implant placement. Materials and Methods: Twenty patients who reported to Rajarajeswari Dental College and Hospital were selected and they were randomly divided into two groups. In both the groups, the selection of the implant size was based on the ridge mapping. In Group I, tooth and tissue-supported surgical stent was fabricated and was used to place the implant. In Group II conventional technique was used for implant placement. Results: In Group I the implant placement was parallel to the adjacent tooth with less variation in the angulation and the mesiodistal orientation of the implant was statistically significant compared to Group II. Conclusion: This technique of stent fabrication is easy, reliable, and cost-effective which also aids in the ideal placement of the implant in terms of orientation and angulation.

Attachment Wear in Different Clear Aligner Therapy: A Comparative Study.

Background: Because of its ease and visual attractiveness, clear aligner therapy has grown in popularity as an orthodontic treatment option. On the other hand, the results of therapy may be impacted by attachment wear. Comparing attachment wear in three distinct clear aligner systems-System A, System B, and System C-was the goal of this investigation. Methods: The research comprised 60 individuals receiving clear aligners as part of their orthodontic treatment. Depending on whatever clear aligner system was being utilised, the patients were split into three groups. Attachment wear was evaluated using a standardised scoring method regularly. The various systems' attachment wear was compared using statistical analysis. Results: Compared to Systems B and C, System A showed less attachment wear. There were notable variations in attachment wear ratings between the systems. System C's attachments had the most wear, whereas System A's attachments had the least. Conclusion: Different clear aligner systems showed varying degrees of attachment wear, with System A exhibiting the least amount of wear. To maximise treatment procedures and guarantee treatment efficacy and longevity in clear aligner therapy, it is vital to comprehend attachment wear patterns.

Biological Control of Stem Rot of Groundnut Induced by Sclerotium rolfsii sacc.

Stem rot of groundnut (Arachis hypogaea L.) caused by Scelrotium rolfsii is the main threat to groundnut production, causing significant economic losses. The present study aims to provide an overview of the potentiality of Trichoderma viride (Tv), Trichoderma harzianum (Th), Pseudomonas fluorescens (Pf), and Bacillus subtilis (Bs), applied either individually or in mixed combination, against Sclerotium rolfsii (isolate SrBKN). The treatment with T. harzianum and P. fluorescens resulted in the highest mycelial growth inhibition (79.61, 83.51, and 86.77%), followed by T. viride and P. fluorescens (75.74, 79.63, and 83.14%). Under in vitro conditions, the combination of bio-agents at 5% culture filtrate proved to be superior against the test pathogen. Seed treatment and soil application of T. harzianum and Pf at 10 (5 + 5) g kg-1 + 10 (5 + 5) kg ha-1, followed by seed treatment and soil application of T. viride and Pf at 10 (5 + 5) g kg-1 + 10 (5 + 5) kg ha-1, resulted in the lowest disease incidence (7.40 and 8.0%), highest disease control (69.37 and 66.88%), maximum dry weight (151 and 147 g plant-1), highest increase in dry weight (75.58 and 70.93), highest pod yield (2665 and 2498 kg ha-1), and highest increase in pod yield (96.38 and 84.08%) under in vivo conditions. We present an effective bio-control-based management module from the lab to the field for the successful control of groundnut stem rot caused by S. rolfsii. Based on the results, it is concluded that the dual formulation of T. harzianum and P. fluorescens, followed by T. viride and P. fluorescens, were the most effective BCAs in suppressing the S. rolfsii. Therefore, an integrated disease management module with these BCAs needs to be developed and validated with a farmers' participatory mode under field conditions.

Isolation and characterization of native antagonistic rhizobacteria against Fusarium wilt of chilli to promote plant growth.

In the eastern coastal regions of Odisha, wilt caused by Fusarium oxysporum f. sp.capsici is an extremely damaging disease in chilli. This disease is very difficult to manage with chemical fungicides since it is soil-borne in nature. The natural rhizosphere soil of the chilli plant was used to isolate and test bacterial antagonists for their effectiveness and ability to promote plant growth. Out of the fifty-five isolates isolated from the rhizosphere of healthy chilli plants, five isolates, namely Iso 01, Iso 17, Iso 23, Iso 24, and Iso 32, showed their highly antagonistic activity against F. oxysporum f. sp. capsici under in vitro. In a dual culture, Iso 32 (73.3%) and Iso 24 (71.5%) caused the highest level of pathogen inhibition. In greenhouse trials, artificially inoculated chilli plants treated with Iso 32 (8.8%) and Iso 24 (10.2%) had decreased percent disease incidence (PDI), with percent disease reduction over control of 85.6% and 83.3%, respectively. Iso 32 and Iso 24 treated chilli seeds have shown higher seed vigor index of 973.7 and 948.8, respectively, as compared to untreated control 636.5. Furthermore, both the isolates significantly increased plant height as well as the fresh and dry weight of chilli plants under the rolled paper towel method. Morphological, biochemical, and molecular characterization identified Bacillus amyloliquefaciens (MH491049) as the key antagonist. This study demonstrates that rhizobacteria, specifically Iso 32 and Iso 24, can effectively protect chilli plants against Fusarium wilt while promoting overall plant development. These findings hold promise for sustainable and eco-friendly management of Fusarium wilt in chilli cultivation.

Postharvest quality and ripening behaviour of un-explored genotypes of Himalayan plain mango diversity.

India is renowned for its mango diversity, with more than 1000 genotypes reported. However, the Himalayan plains bear some elite genotypes which supposed to bear high postharvest value, the systemic postharvest study of which is yet to be attempted. The aim of present study is to evaluate the postharvest quality and ripening behviour of these important genotypes. Thus, 15 un-explored mango genotypes of this region were selected and evaluated for ripening behaviour and detailed postharvest profiling via internal (total phenolic and total flavonoid content), nutritional attributes (Brix: acid ratio, total carotenoid concentration, ascorbic acid content and antioxidant activity), sensory evaluation, fruit softening enzymes (polygalactouronase, pectin methylesterase and lipoxygenase), shelf life attributes (respiration rate, physiological loss in weight and storage life in days) external attributes (fruit weight, fruit firmness, peel thickness, fruit shape and dry seed weight) and mineral contents (Calcium, potassium and phosphorous) under ambient storage (25 ± 4 °C and 65 ± 5 % RH). The results revealed that the highest total flavonoid content (682.40 µg g-1), ascorbic acid (46.88 mg 100 g-1) and antioxidant activity (4.84 µmol TE g-1) exhibited by 'Sukul'. The total phenolic content was recorded as the highest in 'Safed Malda' (510.42 µg GAE g-1 FW), and total carotenoid concentration was recorded as the highest in 'Sipiya' (7.30 mg 100 g-1) 'Zardalu' (7.04 mg 100 g-1) and 'Mithua' (6.98 mg 100 g-1). Interestingly, genotypes such as 'Sukul', Sipiya' and 'Krishna Bhog 'exhibited a 4-5 days higher storage life than other selected genotypes. Screened genotypes exhibited a high diversity of nutritional and biochemical contents. The results of this study bear practical utility for research (quality improvement programme) and the processing industry.

Colourful staples on your table: Unus ex genere suo.

The positive health benefits of colored staples have led to a significant increase in interest in them as healthy food ingredients. Numerous in vitro and in vivo studies have demonstrated that colored cereals are rich in antioxidants, carotenoids, and xanthophylls, which are widely used as natural additives in the food industry. Additionally, shifts in consumer preferences have led to a preference for nutritionally balanced diets over traditional high-energy ones. Thus, colored cereals offer additional nutritional value that has been previously untapped. Besides providing essential nutrients, these natural pigments also have the potential to replace synthetic colors and food additives. This review aims to provide insights into the nutritional value of various colored staples compared to conventional starchy staples and their associated health benefits. Colored staples can be incorporated into daily diets, offering a nutritious and healthful addition to the table.

Evaluation of blood based quantitative PCR as a molecular diagnostic tool for post kala-azar dermal leishmaniasis (PKDL).

BACKGROUND: Post kala-azar dermal leishmaniasis (PKDL) is a consequential dermal manifestation of visceral leishmaniasis (VL), serving as a parasite reservoir. The traditional diagnostic approach, which requires an invasive skin biopsy is associated with inherent risks and necessitates skilled healthcare practitioners in sterile settings. There is a critical need for a rapid, less invasive method for Leishmania detection. The main objective of this study was to evaluate and compare the diagnostic efficacy of PCR and qPCR in detecting PKDL, utilizing both skin and blood samples and to assess the utility of blood samples for molecular diagnosis. METHODS AND RESULTS: 73 individuals exhibiting clinical symptoms of PKDL and who had tested positive for rK39 rapid diagnostic test (RDT) were enrolled in this study. For the diagnosis of PKDL, both PCR and real-time quantitative PCR (qPCR), employing SYBR Green and TaqMan assays, were performed on blood and skin matched samples. qPCR results using both TaqMan and SYBR Green assay, indicated higher parasite loads in the skin compared to blood, as evident by the Ct values. Importantly, when blood samples were used for PKDL diagnosis by qPCR, an encouraging sensitivity of 69.35% (TaqMan assay) and 79.36% (SYBR Green) were obtained, compared to 8.2% with conventional PCR. CONCLUSION: The findings of the study suggest the potential utility of blood for molecular diagnosis by qPCR, offering a less invasive alternative to skin biopsies in field setting for the early detection of parasitaemia in PKDL patients and effective management and control of the disease.

MHC tetramer technology: Exploring T cell biology in health and disease.

Major histocompatibility complex (MHC) tetramers stand as formidable tools within T cell biology, facilitating the exploration and comprehension of immune responses. These artificial molecules, comprising four bound MHC molecules, typically with a specified peptide and a fluorescent label, play a pivotal role in characterizing T cell subsets, monitoring clonal expansion, and unraveling T cell dynamics during responses to infections or immunotherapies. Beyond their applications in T cell biology, MHC tetramers prove valuable in investigating a spectrum of diseases such as infectious diseases, autoimmune disorders, and cancers. Their instrumental role extends to vaccine research and development. Notably, when appropriately configured, tetramers transcend T cell biology research and find utility in exploring natural killer T cells and contributing to specific T cell clonal deletions.