RESUMEN
1. Site directed mutagenesis was used to alter the structure of Torpedo californica nicotinic acetylcholine receptor (nAChR) and to identify amino acid residues which contribute to noncompetitive inhibition by quinacrine. Mutant receptors were expressed in Xenopus laevis oocytes injected with in vitro synthesized mRNA and the whole cell currents induced by acetylcholine (ACh) were recorded by two electrode voltage clamp. 2. A series of mutations of a highly conserved Arg at position 209 of the alpha subunit of Torpedo californica nAChR revealed that positively charged amino acids are required for functional receptor expression. Mutation of Arg to Lys (alpha R209K) or His (alpha R209H) at position 209 shifted the EC50 for ACh slightly from 5 microM to 12 microM and increased the normalized maximal channel activity 8.5- and 3.2-fold, respectively. 3. These mutations altered the sensitivity of nAChR to noncompetitive inhibition by quinacrine. The extent of inhibition of ion channel function by quinacrine was decreased as pH increased in both wild type and mutant nAChR suggesting that the doubly charged form of quinacrine was responsible for the inhibition. 4. Further mutations at different positions of the alpha subunit suggest the contribution of Pro and Tyr residues at positions 211 and 213 to quinacrine inhibition whereas mutations alpha I210A and alpha L212A did not have any effects. None of these mutations changed the sensitivity of nAChR to inhibition by a different noncompetitive inhibitor, chlorpromazine. 5. These findings support a hypothesis that the quinacrine binding site is located in the lumen of the ion channel. In addition, the quantitative effect of point mutations at alternate positions on the sensitivity of quinacrine inhibition suggests that the secondary structure at the beginning of M1 region might be beta sheet structure.
Asunto(s)
Mutagénesis Sitio-Dirigida , Antagonistas Nicotínicos/farmacología , Quinacrina/farmacología , Receptores Nicotínicos/genética , Acetilcolina/farmacología , Secuencia de Aminoácidos , Aminoácidos/fisiología , Animales , Bungarotoxinas/farmacología , Clorpromazina/farmacología , Inhibidores Enzimáticos/farmacología , Concentración de Iones de Hidrógeno , Datos de Secuencia Molecular , Receptores Nicotínicos/ultraestructura , Sensibilidad y Especificidad , TorpedoRESUMEN
beta-Lactamase from Bacillus licheniformis forms a stable compact intermediate state at low pH and moderate salt concentration (the A state), with properties consistent with a molten globule. A single cysteine residue was introduced into this class A beta-lactamase by site-directed mutagenesis at position 166. A spin label was attached to the thiol of this cysteine residue via a disulfide bond as a probe of the side-chain mobility. The mutant protein and the spin-labeled derivative exhibited similar conformational properties to the wild-type enzyme at acidic pH. The A state induced by chloride or trichloroacetate (TCA) anions was characterized by circular dichroism and esr. The A state at pH 0.5 (0.32 M HCl), or at pH 2 in the presence of 8 mM TCA or 0.4 M Cl-, had comparable amounts of secondary structure to the native state but lacked significant tertiary structure, as judged by the lack of near-UV circular dichroism. Analysis of the esr spectral line widths showed that the mobility of the spin label in the A state was similar to that in the native state and much less mobile than in the unfolded state, indicating significant constraints on the side-chain mobility in this region of the molecule in the A state. The implications of this finding to the structure of the A state are discussed.
Asunto(s)
Conformación Proteica , beta-Lactamasas/química , Secuencia de Aminoácidos , Bacillus/enzimología , Dicroismo Circular , Cisteína , Espectroscopía de Resonancia por Spin del Electrón , Concentración de Iones de Hidrógeno , Mutagénesis Sitio-Dirigida , Desnaturalización Proteica , Pliegue de Proteína , Proteínas Recombinantes/química , Espectrofotometría Ultravioleta , Marcadores de Spin , Termodinámica , Urea , beta-Lactamasas/genética , beta-Lactamasas/metabolismoRESUMEN
Short alanine peptides, containing 16 or 17 residues, appear to form alpha-helices in aqueous solution. But the main spectroscopic analyses used on helical peptides (circular dichroism and nuclear magnetic resonance) cannot distinguish between an alpha-helix (in which the ith residue is hydrogen-bonded to residue i + 4; ref. 9) and the next most common peptide helix, the 3(10)-helix10 (i-->i + 3 hydrogen-bonding). To address this problem we have designed single and doubly spin-labelled analogues of alanine-based peptides in which the nitroxide spin label forms an unbranched side chain extending from the sulphur atom of a cysteine residue. Here we report the circular dichroism, Fourier-transform infrared and electron-spin resonance spectra of these peptides under helix-forming conditions. The infrared absorbance gives an amide I' band with a frequency that is substantially different from that observed for alpha-helices. The electron-spin resonance spectra of doubly labelled helices show that the ranking of distances between side chains, around a single turn (residues 4-8), is inconsistent with an alpha-helical structure. Our experiments suggest that the more likely peptide geometry is a 3(10)-helix.
Asunto(s)
Alanina/química , Péptidos/química , Estructura Secundaria de Proteína , Secuencia de Aminoácidos , Dicroismo Circular , Espectroscopía de Resonancia por Spin del Electrón , Análisis de Fourier , Datos de Secuencia Molecular , Espectrofotometría Infrarroja , Marcadores de SpinRESUMEN
We have used electron spin resonance and circular dichroism to examine and compare the dynamics in two analogues of the Ala-based 3K(I) peptide [Marqusee, S., Robbins, V.H., & Baldwin, R. L. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 5286-5290], labeled at positions 4 and 8, throughout the alpha-helix----coil transition. In the middle of the thermal unfolding transition, our results demonstrate that the local mobility near the N-terminus is greater than at the center of the peptide. This provides evidence, from the perspective of dynamics, that the ends of Ala-based alpha-helices are frayed. We further find that the position dependence of the mobility for the thermally unfolded state differs from that of the denaturant unfolded state. Only the latter state exhibits the local dynamics expected for a genuine random coil.
Asunto(s)
Péptidos/química , Alanina/química , Secuencia de Aminoácidos , Dicroismo Circular , Espectroscopía de Resonancia por Spin del Electrón , Glicina/química , Datos de Secuencia Molecular , Movimiento (Física) , Péptidos/síntesis química , Conformación Proteica , Relación Estructura-Actividad , TemperaturaRESUMEN
A series of short alanine-based synthetic peptides (16 or 17 residues) have previously been shown to exhibit an anomalously high degree of alpha-helicity [Marqusee, S., et al. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 5286-5290; Marqusee, S., & Baldwin, R.L. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 8898-8902]. These peptides are ideal models for extracting position-dependent structural and dynamic information. Using the methanethiosulfonate nitroxide spin label (MTSSL), we labeled an analogue of the salt-bridge-stabilized "i+4" peptide, called the "i+4c", which has a specific attachment site created by replacing the central alanine with a cysteine. Circular dichroism (CD) spectra demonstrate that the i+4c-MTSSL peptide retains nearly the same helicity as the original i+4 peptide. The ESR spectra of the labeled peptide indicate no significant aggregation. ESR spectra were acquired throughout the helix-coil transition by temperature variation. From the motionally narrowed spectra, we extracted the rotational correlation times of the nitroxide label. Parallel measurements with circular dichroism enabled us to relate these parameters directly to the fractional helicity. For comparison, we followed a similar procedure with MTSSL-labeled glutathione (GS-MTSSL), a tripeptide that does not form an alpha-helix. Our results are interpreted in terms of a local tumbling volume, V(L), which reflects the portion of the peptide that reorients with the nitroxide label. At high fractional helicity, V(L) is similar to the volume expected for a 17-residue helix.
Asunto(s)
Péptidos/química , Secuencia de Aminoácidos , Dicroismo Circular , Simulación por Computador , Espectroscopía de Resonancia por Spin del Electrón , Glutatión/química , Mesilatos , Modelos Moleculares , Conformación Proteica , Marcadores de Spin , Relación Estructura-ActividadRESUMEN
The molecularity of the ion channel formed by peptide fragments of colicin has taken on particular significance since the length of the active peptide has been shown to be less than 90 amino acids and the lumen size at least 8 A. Cell survival experiments show that killing by colicin obeys single-hit statistics, and ion leakage rates from phospholipid vesicles are first order in colicin concentration. However, interpretation in molecular terms is generally complicated by the requirement of large numbers of colicin molecules per cell or vesicle. We have measured the discharge of potential across membranes of small phospholipid vesicles by following the changes in binding of potential sensitive spin labeled phosphonium ions as a function of the number of colicin fragments added. Because of the sensitivity of the method, it was possible to reliably investigate the effect of colicin in a range where there was no more than 0.2 colicins per vesicle. The quantitative results of these experiments yield a direct molecular stoichiometry and demonstrate that one C-terminal fragment of the colicin molecule per one vesicle is sufficient to induce a rapid ion flux in these vesicles. In addition, the experiments confirm earlier findings that the colicin fragments do not migrate from one vesicle to another at pH 4.5. Similar results are obtained with large unilamellar vesicles.
Asunto(s)
Colicinas/farmacología , Canales Iónicos , Espectroscopía de Resonancia por Spin del Electrón , Potenciales de la Membrana , Fragmentos de Péptidos/farmacología , Fosfolípidos/metabolismo , TripsinaRESUMEN
Permeabilities for a homologous series of amine and carboxylate nitroxide spin probes were measured in human red blood cells by an electron paramagnetic resonance (EPR) method. Permeabilities determined in this study are much lower than would be predicted for a sheet of bulk hydrocarbon and the polarity of the rate-limiting region is shown to be greater than bulk hydrocarbon. This suggests that the rate-limiting region for permeation of these nonelectrolytes is somewhere in the membrane periphery rather than in the center of the membrane. The red cell membrane does not discriminate between these probes on the basis of molecular volume, as might be predicted by a simple free-volume theory of membrane permeation.
Asunto(s)
Aminas/farmacocinética , Ácidos Carboxílicos/farmacocinética , Espectroscopía de Resonancia por Spin del Electrón , Membrana Eritrocítica/metabolismo , Permeabilidad de la Membrana Celular , HumanosRESUMEN
Permeabilities for an homologous series of amine nitroxide spin probes were measured in liposomes of varying composition by an electron paramagnetic resonance (EPR) method. Results show that the rate-limiting step in permeation is not adsorption/desorption at the aqueous/membrane interface for two probes in phosphatidylcholine/phosphatidic acid liposomes and for one probe in phosphatidylcholine/cholesterol/phosphatidic acid liposomes. Accordingly, we interpret observed selectivity patterns for the entire series of probes in liposomes and red cells in terms of the properties of the bilayer interior. Results are inconsistent with simple applications of either free volume or hydrocarbon sheet models of nonelectrolyte permeation. In the former case, it was found that liposomes do not select against these probes on the basis of molecular volume. In the latter case, probe permeabilities are all much lower than would be predicted for a sheet of bulk hydrocarbon and the polarity of the rate-limiting region is shown to be greater than bulk hydrocarbon. Together with the results of previous studies of spin-labeled solutes in membranes, as well as studies of lipid dynamics in membranes, these latter results suggest that the rate-limiting region in nonelectrolyte permeation is not in the center of the bilayer, but in the relatively ordered acyl chain segments near the glycerol backbone.
Asunto(s)
Aminas/farmacocinética , Espectroscopía de Resonancia por Spin del Electrón , Liposomas/metabolismo , Permeabilidad de la Membrana CelularRESUMEN
Colicin E1 is an E. coli plasmid-encoded water-soluble protein that spontaneously inserts into lipid membranes to form a voltage-gated ion channel. We have employed a novel approach in which site-directed mutagenesis is used to provide highly specific attachment points for nitroxide spin labels. A series of colicin mutants, differing only by the position of a single cysteine residue, were prepared and selectively labeled at that cysteine. A hydrophilic sequence (398-406) within the C-terminal domain of the water-soluble form of the protein was investigated and exhibited an electron paramagnetic resonance (EPR) spectral periodicity strongly suggesting an amphiphilic alpha-helix. After removal of the N-terminus of the protein with trypsin, the spectra for this sequence indicate increased label mobility and a more flexible structure.
Asunto(s)
Colicinas , Marcadores de Spin , Secuencia de Aminoácidos , Cisteína/genética , Espectroscopía de Resonancia por Spin del Electrón , Escherichia coli/genética , Datos de Secuencia Molecular , Mutación , Óxidos de Nitrógeno , Oxalatos , Ácido Oxálico , Conformación Proteica , TripsinaRESUMEN
A method is described for the handling of tissue samples for copper determination by neutron activation analysis. The results obtained for normal liver copper estimations compared favourably with results obtained by conventional methods.