RESUMEN
We have constructed a public gene expression data repository and online data access and analysis, WWW and FTP sites for serial analysis of gene expression (SAGE) data. The WWW and FTP components of this resource, SAGEmap, are located at http://www.ncbi.nlm.nih. gov/sage and ftp://ncbi.nlm.nih.gov/pub/sage, respectively. We herein describe SAGE data submission procedures, the construction and characteristics of SAGE tags to gene assignments, the derivation and use of a novel statistical test designed specifically for differential-type analyses of SAGE data, and the organization and use of this resource.
Asunto(s)
Bases de Datos Factuales , Expresión Génica/genética , Internet , Femenino , Biblioteca de Genes , Humanos , Masculino , Análisis de Secuencia de ADN/métodos , Lugares Marcados de Secuencia , Procesamiento de Señales Asistido por ComputadorAsunto(s)
Perfilación de la Expresión Génica/métodos , Neoplasias de la Próstata/genética , Bases de Datos Factuales , Expresión Génica , Perfilación de la Expresión Génica/estadística & datos numéricos , Biblioteca de Genes , Humanos , Masculino , Análisis de Secuencia por Matrices de Oligonucleótidos , Patología Clínica/métodos , Polimorfismo de Nucleótido SimpleAsunto(s)
Secuencia de Bases , ADN/genética , Bases de Datos Factuales , Animales , ADN/química , Expresión Génica , HumanosRESUMEN
A computer program, OLIGO, is described which was designed to manage projects involving the synthesis of large numbers of oligonucleotide fragments using cellulose discs as immobilizing support. The program performs the functions of data entry, verification, analysis and modification; the sorting of filters before each step and project status updating after each reaction step; output of the project status at any point and storage of the finalized project data in a permanent reference file.
Asunto(s)
Computadores , Oligonucleótidos/síntesis química , Programas Informáticos , Secuencia de Bases , Celulosa , FiltraciónRESUMEN
A set of computer programs is described which constitutes a clone database management system. Maintenance of the database and the stocks of material is designed to be under the control of one person or group of people, who may insert, delete or modify data entries, and who may interrogate the database as to which stocks are in need of checking. The system is organised in such a way that information is freely and speedily available to all users. Database entries may be accessed by name or key word.
Asunto(s)
Clonación Molecular , ADN Recombinante , Animales , Bacteriófagos/genética , Pollos , Computadores , Enzimas de Restricción del ADN , Recolección de Datos , Eritrocitos , Escherichia coli/genética , Genes , PlásmidosRESUMEN
A computer program (RSITE) was developed which predicts the recognition sequence of a restriction endonuclease. The sizes of fragments experimentally determined on cleavage of a DNA of known sequence were input. Possible recognition sequences producing fragments of sizes matching those determined empirically were printed out. The program faithfully predicted the specificity of restriction enzymes of known recognition sequence and also determined the recognition sequence of a new restriction enzyme from Haemophilus influenzae GU (HinGU II).