RESUMEN
Traceability ensures a link between carcass, quarters or cuts of beef and the individual animal or the group of animals from which they are derived. Meat traceability is an essential tool for successful identification and recall of contaminated products from the market during a food crisis. Meat traceability is also extremely important for protection and value enhancement of good-quality brands. Molecular meat traceability would allow verification of conventional methods used for beef tracing in synthetic Mexican bovine breeds. We evaluated a set of 11 microsatellites for their ability to identify animals belonging to these synthetic breeds, Brangus and Charolais/Brahman (78 animals). Seven microsatellite markers allowed sample discrimination with a match probability, defined as the probability of finding two individuals sharing by chance the same genotypic profile, of 10(-8). The practical application of the marker set was evaluated by testing eight samples from carcasses and pieces of meat at the slaughterhouse and at the point of sale. The DNA profiles of the two samples obtained at these two different points in the production-commercialization chain always proved that they came from the same animal.
Asunto(s)
Bovinos/genética , Dermatoglifia del ADN/métodos , Genotipo , Repeticiones de Microsatélite/genética , Animales , Cruzamiento , Femenino , Masculino , Carne , Industria para Empaquetado de Carne/métodos , Recall y Retirada del ProductoRESUMEN
This research was aimed at evaluating the inhibition of oxidative changes of beef patties packaged in modified atmosphere (70% O(2)+20% CO(2)+10% N(2)) by natural antioxidants: ascorbic acid (500 ppm), taurine (50 mM), carnosine (50 mM), rosemary powder (1000 ppm) and their combinations with the first. Beef patties stored at 2±1°C for 20 days were evaluated for colour (L*, a*, b*, C* and H*), TBARS, metmyoglobin formation (% of total myoglobin), psychrotrophic microbial counts and sensory odour and discolouration. Rosemary, either alone or with ascorbic acid, was highly effective in inhibiting both metmyoglobin formation and lipid oxidation; sensory analysis was in agreement with these results. Ascorbic acid, ascorbic acid+taurine and ascorbic acid+carnosine treatments showed a limited inhibitory effect of myoglobin oxidation, while carnosine and carnosine+ascorbic acid were effective in inhibiting lipid oxidation. Taurine alone failed to exert any antioxidant effect. Principal components analysis confirmed these results.
RESUMEN
En el planeta existe una gran variedad de setas, muchas de ellas tóxicas e incluso mortales. La intoxicación alimentaria por la ingestión de hongos (micetismo) es un problema muy difundido cuyo diagnóstico se confunde o pasa inadvertido. Se señalan los estados de la República en donde el mecetismo se presenta con mayor frecuencia, se alude a su cuadro clínico, diagnóstico y tratamiento