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1.
Biotechnol Bioeng ; 77(7): 752-7, 2002 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-11835135

RESUMEN

Thirteen glycosidases of microbial origin and almond beta-glycosidase were assayed in octanol/DMF (80:20, v/v), using a combination of hydrolysis, transglycosylation, and condensation reactions, in order to assess their potential for the production of alkyl glucosides. The two mesophile enzymes were highly impaired by the organic media. Three of the 11 thermophile enzymes gave interesting results in the hydrolysis and transglycosylation reactions, but they were highly inhibited by glucose. This made their use in a condensation reaction less interesting than the use of almond beta-glucosidase, which has a lower activity but shows less inhibition by the glucose.


Asunto(s)
Bacterias Anaerobias/enzimología , Candida/enzimología , Glucósidos/análisis , Glucósidos/biosíntesis , Glicósido Hidrolasas/análisis , Nueces/enzimología , Animales , Bovinos , Glicósido Hidrolasas/metabolismo , Glicosilación , Hidrólisis , Prunus , Factores de Tiempo
2.
J Biosci Bioeng ; 93(6): 557-62, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-16233249

RESUMEN

We attempted to isolate microorganisms able to metabolize (S)-baclofen while showing little or no activity with (R)-baclofen. A culture collection of 218 microbial isolates was established from several soil samples using various approaches. Subsequent chiral HPLC analysis revealed that only two cultures showed promise. Initial shake flask experiments demonstrated a correlation between culture growth and (S)-baclofen metabolism. (S)-baclofen was metabolized much more rapidly than (R)-baclofen. The best culture, identified as Streptomyces halstedii, was used to scale up the process in small-scale fed-batch fermentations using pulse addition of racemic baclofen to yield extracellular fractions showing enantiomeric excess (e.e.) values of greater than 90% in favour of the (R)-isomer. Resting cells also were able to carry out the wanted reaction but initial attempts using cell free extracts were negative. It is speculated that the initial attack on (S)-baclofen may be catalyzed by a mono- or dioxygenase.

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