RESUMEN
Moths and butterflies (Lepidoptera) are the most species-rich group of animals with female heterogamety, females mostly having a WZ, males a ZZ sex chromosome constitution. We studied chromatin conformation, activity, and inactivity of the sex chromosomes in the flour moth Ephestia kuehniella and the silkworm Bombyx mori, using immunostaining with anti-H3K9me2/3, anti-RNA polymerase II, and fluoro-uridine (FU) labelling of nascent transcripts, with conventional widefield fluorescence microscopy and 'spatial structured illumination microscopy' (3D-SIM). The Z chromosome is euchromatic in somatic cells and throughout meiosis. It is transcriptionally active in somatic cells and in the postpachaytene stage of meiosis. The W chromosome in contrast is heterochromatic in somatic cells as well as in meiotic cells at pachytene, but euchromatic and transcriptionally active like all other chromosomes at postpachytene. As the W chromosomes are apparently devoid of protein-coding genes, their transcripts must be non-coding. We found no indication of 'meiotic sex chromosome inactivation' (MSCI) in the two species.
Asunto(s)
Cromatina/metabolismo , Mariposas Nocturnas/genética , Cromosomas Sexuales/metabolismo , Animales , Bombyx/genética , Bombyx/metabolismo , Cromosomas de Insectos/metabolismo , Meiosis , Mariposas Nocturnas/metabolismoRESUMEN
The speciose insect order Lepidoptera (moths and butterflies) and their closest relatives, Trichoptera (caddis flies), share a female-heterogametic sex chromosome system. Originally a Z/ZZ (female/male) system, it evolved by chromosome rearrangement to a WZ/ZZ (female/male) system in the most species-rich branch of Lepidoptera, a monophyletic group consisting of Ditrysia and Tischeriina, which together comprise more than 98% of all species. Further sporadic rearrangements created multi-sex chromosome systems; sporadic losses of the W changed the system formally back to Z/ZZ in some species. Primary sex determination depends on a Z-counting mechanism in Z/ZZ species, but on a female-determining gene, Fem, in the W chromosome of the silkworm. The molecular mechanism is unknown in both cases. The silkworm shares the last step, dsx, of the hierarchical sex-determining pathway with Drosophila and other insects investigated, but probably not the intermediate steps between the primary signal and dsx. The W chromosome is heterochromatic in most species. It contains few genes and is flooded with interspersed repetitive elements. In interphase nuclei of females it is readily discernible as a heterochromatic body which grows with increasing degree of polyploidy in somatic cells. It is used as a marker for the genetic sex in studies of intersexes and Wolbachia infections. The sex chromosome system is being exploited in economically important species. Special strains have been devised for mass rearing of male-only broods in the silkworm for higher silk production and in pest species for the release of sterile males in pest management programs.
Asunto(s)
Lepidópteros/genética , Cromosomas Sexuales/genética , Procesos de Determinación del Sexo , Animales , Bombyx/genética , Mariposas Diurnas/genética , Núcleo Celular/genética , Intercambio Genético/genética , Compensación de Dosificación (Genética) , Evolución Molecular , Femenino , Interfase , Cariotipificación , Masculino , Meiosis , Mariposas Nocturnas/genética , Filogenia , Cromatina Sexual/genéticaRESUMEN
In somatic tissues, the mouse Ki-67 protein (pKi-67) is expressed in proliferating cells only. Depending on the stage of the cell cycle, pKi-67 is associated with different nuclear domains: with euchromatin as part of the perichromosomal layer, with centromeric heterochromatin, and with the nucleolus. In gametes, sex-specific expression is evident. Mature MII oocytes contain pKi-67, whereas pKi-67 is not detectable in mature sperm. We investigated the re-establishment of the cell cycle-dependent distribution of pKi-67 during early mouse development. After fertilization, male and female pronuclei exhibited very little or no pKi-67, while polar bodies were pKi-67 positive. Towards the end of the first cell cycle, prophase chromosomes of male and female pronuclei simultaneously got decorated with pKi-67. In 2-cell embryos, the distribution pattern changed, presumably depending on the progress of development of the embryo, from a distribution all over the nucleus to a preferential location in the nucleolus precursor bodies (NPBs). From the 4-cell stage onwards, pKi-67 showed the regular nuclear relocations known from somatic tissues: during mitosis the protein was found covering the chromosome arms as a constituent of the perichromosomal layer, in early G1 it was distributed in the whole nucleus, and for the rest of the cell cycle it was associated with NPBs or with the nucleolus.
Asunto(s)
Proliferación Celular , Embrión de Mamíferos/metabolismo , Desarrollo Embrionario , Antígeno Ki-67/biosíntesis , Animales , Biomarcadores/metabolismo , Núcleo Celular/metabolismo , Embrión de Mamíferos/citología , Femenino , Fertilización , Técnica del Anticuerpo Fluorescente , Masculino , Ratones , Oocitos/metabolismo , Espermatozoides/metabolismo , Cigoto/metabolismoRESUMEN
Comparative Genome Hybridization (CGH) can be used as a universal method for the identification of molecularly differentiated sex chromosomes. This is profitable in species with homomorphic sex chromosomes or when chromosomes are unfavourable for cytogenetics, e.g. when size differences are insufficient, chromosomes numerous and/or banding methods fail. In this method, genomic DNA from females competes as a probe with that from males for binding to the chromosome targets. Easy extraction and labelling methods afford a method that can be applied even when few specimens are available, e.g. when specimens for investigation have to be collected in the field - CGH also offers the possibility to obtain a rough estimate of the DNA composition of the sex chromosome.
Asunto(s)
Evolución Biológica , Genómica/métodos , Hibridación in Situ/métodos , Cromosomas Sexuales/genética , Animales , Femenino , Masculino , Procesos de Determinación del SexoRESUMEN
By fluorescence in situ hybridization, we mapped the location of genes associated with the Sp100-rs cluster, a long-range repeat cluster in chromosome 1 of the house mouse, Mus musculus. The cluster comprises between 60 and 2000 repeats and extends over 6-200 Mb of the M. musculus genome, depending on the source of the cluster. The cluster evolved during the last two million years in the genus Mus in the lineage to which M. musculus belongs. The Asiatic mouse species M. caroli is not in this lineage and does not possess the cluster. M. caroli represents the ancestral genomic organization of the cluster source components Sp100, Csprs and Ifi75: they are located close to each other in the same chromosome band (1D). However, Sp100-rs, the principal gene of the cluster, is not present in the M. caroli genome. It is a chimeric M. musculus gene that arose by fusion of Csprs and the 5' part of Sp100. Sp100-rs and Ifi75 are homogeneously distributed throughout the cluster while Sp100 and Csprs in its original sequence context flank the cluster on opposite sides. Our results suggest a model for the origin and evolution of the long-range repeat cluster by duplication, gene fusion and amplification.
Asunto(s)
Evolución Molecular , Ratones/genética , Secuencias Repetitivas de Ácidos Nucleicos , Animales , Mapeo Cromosómico , Hibridación Fluorescente in SituRESUMEN
We identified and analyzed the genes Sp100, Csprs, and Ifi75 in two members of the genus Mus, M. musculus and M. caroli. Sp100 is a nuclear dot gene; Csprs and Ifi75 are novel genes encoding a putative G-protein coupled receptor (GPCR) and a putative transcriptional coactivator, respectively. A fourth gene, Sp100-rs, occurs in M. musculus, but not in M. caroli. Sp100-rs is a chimeric gene which arose by fusion of Sp100 and Csprs copies. Sp100-rs and Ifi75 are components of a repeat cluster that extends over 6-200 Mb of the M. musculus genome. The Sp100-rs fusion gene arose only 1-2 million years ago and has become fixed and amplified in M. musculus. Although the gene is transcribed, it appears to have no function. The repeat cluster may have become fixed in the species as a 'hitchhiker' in a 'selective sweep'.
Asunto(s)
Antígenos Nucleares , Ratones/genética , Familia de Multigenes/genética , Proteínas Nucleares/genética , Receptores de Superficie Celular/genética , Receptores Acoplados a Proteínas G , Transactivadores/genética , Animales , Secuencia de Bases , Clonación Molecular , Evolución Molecular , Exones/genética , Dosificación de Gen , Genoma , Ratones/clasificación , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Alineación de SecuenciaRESUMEN
We describe SC complements and results from comparative genomic hybridization (CGH) on mitotic and meiotic chromosomes of the zebrafish Danio rerio, the platyfish Xiphophorus maculatus and the guppy Poecilia reticulata. The three fish species represent basic steps of sex chromosome differentiation: (1) the zebrafish with an all-autosome karyotype; (2) the platyfish with genetically defined sex chromosomes but no differentiation between X and Y visible in the SC or with CGH in meiotic and mitotic chromosomes; (3) the guppy with genetically and cytogenetically differentiated sex chromosomes. The acrocentric Y chromosomes of the guppy consists of a proximal homologous and a distal differential segment. The proximal segment pairs in early pachytene with the respective X chromosome segment. The differential segment is unpaired in early pachytene but synapses later in an 'adjustment' or 'equalization' process. The segment includes a postulated sex determining region and a conspicuous variable heterochromatic region whose structure depends on the particular Y chromosome line. CGH differentiates a large block of predominantly male-specific repetitive DNA and a block of common repetitive DNA in that region.
Asunto(s)
Ciprinodontiformes/genética , Evolución Molecular , Poecilia/genética , Cromosomas Sexuales , Pez Cebra/genética , Animales , Diploidia , Femenino , Cariotipificación , Masculino , Meiosis , Mitosis , Hibridación de Ácido Nucleico , Cromosomas Sexuales/ultraestructura , Especificidad de la Especie , Complejo Sinaptonémico/ultraestructuraRESUMEN
The physical basis of non-Mendelian segregation of a sex-linked marker was studied in sex- chromosome mutant females of eight ASF ('abnormal segregating females') lines in the flour moth, Ephestia kuehniella. Electron microscopical analysis of microspread synaptonemal complexes revealed that in one line, the Z chromosome segment that contained the dz+ allele was translocated onto an autosome. The resulting quadrivalent visible in early female meiosis was 'corrected' into two bivalents in later stages. This explains autosomal inheritance of the sex chromosome marker in this strain. In the other seven ASF lines, the type of meiotic pairing of an additional fragment (Zdz+) of the Z chromosome was responsible for abnormal segregation of the marker gene. In several of these lines, Zdz+ contained a piece of the W chromosome in addition to the Z segment, as was confirmed by comparative genomic hybridization (CGH). Zdz+ formed three alternative pairing configurations with the original sex chromosomes: (i) a WZZdz+ trivalent, (ii) a WZ bivalent and a Zdz+ univalent or (iii) a ZZdz+ bivalent and a W univalent. In the most frequent WZZdz+ configuration, Zdz+ synapsed with Z and, consequently, segregated with W, simulating W linkage. This explains the predominant occurrence of the parental phenotypes in the progeny. Zdz+ univalents or W univalents, on the other hand, segregated randomly, resulting in both parental and nonparental phenotypes. In two of these lines, the Zdz+ was transmitted only to females. The results suggest that the W chromosome segment in Zdz+ of these lines contains a male-killing factor which makes it incompatible with male development. Our data provide direct evidence for the regular transmission of radiation-induced fragments from lepidopteran chromosomes through more than 50 generations. This is facilitated by the holokinetic nature of lepidopteran chromosomes. We conclude that Zdz+ fragments may persist as long as they possess active kinetochore elements.
Asunto(s)
Emparejamiento Cromosómico/fisiología , Marcadores Genéticos , Meiosis/fisiología , Mariposas Nocturnas/genética , Cromosomas Sexuales/fisiología , Animales , Femenino , Masculino , Mariposas Nocturnas/fisiología , Cromosomas Sexuales/ultraestructura , Complejo Sinaptonémico/ultraestructuraRESUMEN
We describe TROMB, a new LTR retrotransposon, from the phorid fly Megaselia scalaris. Three full-length copies (4226, 4160 and 4129bp) and a truncated one (319bp) have been isolated. The target site consensus is TATAT, with a 4bp target site duplication TATA. The LTRs are short (142bp) and contain a TATA-box and a polyadenylation signal. The isolated copies are degenerate to different degrees and presumably inactive. The polyprotein coding sequence contains scattered stop codons and deletions/insertions at non-homologous positions. The consensus sequence among the three full-length copies, however, has an uninterrupted open reading frame and, presumably, represents the original sequence of the active element. Southern hybridization experiments showed TROMB to be present at a low copy number in two wild-type strains of M. scalaris and absent in a related species, M. abdita. The order of domains in the polyprotein coding region, the target site specificity for AT-rich sequences, and the protein sequence similarity to blastopia, mdg3 and micropia place TROMB in the gypsy-Ty3 group of LTR retrotransposons.
Asunto(s)
Dípteros/genética , Retroelementos/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN/química , ADN/genética , Evolución Molecular , Femenino , Dosificación de Gen , Masculino , Datos de Secuencia Molecular , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Secuencias Repetidas TerminalesRESUMEN
Sex-lethal (Sxl) is Drosophila melanogaster's key regulating gene in the sex-determining cascade. Its homologue in Megaselia scalaris, the chromosome 3 gene Megsxl, codes for a protein with an overall similarity of 77% with the corresponding D. melanogaster sequence. Expression in M. scalaris, however, is very unlike that in D. melanogaster. Megsxl transcripts with a long ORF occur in both sexes. Differential splicing is conserved but not sex-specific. There are several splice variants, among them one is common to gonads and somatic tissues of all developmental stages investigated, one is specific for ovaries and embryos, and a third one is not found in ovaries. In the ovary, Megsxl is heavily transcribed in nurse cells and transported into eggs. These results suggest a non-sex-determining function during early embryogenesis; the presence of Megsxl RNA in testes and somatic tissues calls for other (or more) functions.
Asunto(s)
Dípteros/genética , Proteínas de Drosophila , Proteínas de Unión al ARN/genética , Factores de Edad , Empalme Alternativo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Clonación Molecular , Secuencia Conservada , ADN Complementario/genética , Drosophila melanogaster/genética , Biblioteca de Genes , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Proteínas de Unión al ARN/biosíntesis , Proteínas de Unión al ARN/fisiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Procesos de Determinación del Sexo , Factores SexualesRESUMEN
The house mouse strain C57BL/6 harbours 64 copies of the multicopy gene Sp100-rs. Three of these are contained in the yeast artificial chromosome (YAC) clone yMm75. Four Sp100-rs transcripts of 3.0, 2.6, 1.6 and 1.3kb were detected by Northern hybridization in the yMm75-harbouring line of Saccharomyces cerevisiae. Additional and less abundant transcripts were detected by RT-PCR. With one exception, the YAC-derived Sp100-rs transcripts were a subset of those found in the C57BL/6 mouse. This indicates transcription and proper splicing of murine pre-mRNAs in yeast. Analysis of the splice sites shows that the yeast splicing machinery accepts splice sites that deviate from the standard yeast consensus sequences. It may be feasible, therefore, at least in a fair proportion of cases, to exploit the mammalian mRNAs present in transgenic yeast for gene recognition of YAC-inserts.
Asunto(s)
Antígenos Nucleares , Proteínas Nucleares , Proteínas/genética , Empalme del ARN , Saccharomyces cerevisiae/genética , Transcripción Genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Northern Blotting , Cromosomas Artificiales de Levadura , ADN Complementario/genética , Regulación de la Expresión Génica , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , ARN/genética , ARN/metabolismo , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
The well-known sex-determining cascade of Drosophila melanogaster serves as a paradigm for the pathway to sexual development in insects. But the primary sex-determining signal and the subsequent step, Sex-lethal (Sxl), have been shown not to be functionally conserved in non-Drosophila flies. We isolated doublesex (dsx), which is a downstream step in the cascade, from the phorid fly Megaselia scalaris, which is a distant relative of D. melanogaster. Conserved properties, e.g., sex-specific splicing, structure of the female-specific 3' splice site, a splicing enhancer region with binding motifs for the TRA2/RBP1/TRA complex that activates female-specific splicing in Drosophila, and conserved domains for DNA-binding and oligomerization in the putative DSX protein, indicate functional conservation of dsx in M. scalaris. Hence, the dsx step of the sex-determining pathway appears to be conserved among flies and probably in an even wider group of insects, as the analysis of a published cDNA from the silkmoth indicates.
Asunto(s)
Secuencia Conservada/genética , Proteínas de Unión al ADN/genética , Dípteros/genética , Proteínas de Drosophila , Proteínas de Insectos/genética , Empalme del ARN/genética , Procesos de Determinación del Sexo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario/análisis , Proteínas de Unión al ADN/clasificación , Evolución Molecular , Exones , Dosificación de Gen , Proteínas de Insectos/clasificación , Intrones , Datos de Secuencia Molecular , Filogenia , ARN/análisis , Secuencias Reguladoras de Ácidos Nucleicos , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido NucleicoRESUMEN
We studied the occurrence of the TTAGG telomere repeats by fluorescence in-situ hybridization (FISH) and Southern hybridization in ten insect species and two other arthropods. (TTAGG)n-containing telomeres were found in three Lepidoptera species, the silkworm Bombyx mori (in which the telomeric sequence was recently discovered), the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella, in one species of Hymenoptera, the honey bee Apis mellifera, in one species of Coleoptera, the bark beetle Ips typographus, in one species of Orthoptera, the locust Locusta migratoria, and in a crustacean, the amphipod Gammarus pulex. They were absent in another species of Coleoptera, the mealworm Tenebrio molitor, two representatives of Diptera, Drosophila melanogaster and Megaselia scalaris, a species of Heteroptera, the bug Pyrrhocoris apterus and a spider, Tegenaria ferruginea. Our results, which confirm and extend earlier observations, suggest that (TTAGG)n was a phylogenetically ancestral telomere motif in the insect lineage but was lost independently in different groups, being replaced probably by other telomere motifs. In the Coleoptera this must have happened rather recently as even members of the same family, Curculionidae, differ with respect to the telomeric DNA.
Asunto(s)
Artrópodos/genética , Genes de Insecto , Telómero/genética , Animales , Southern Blotting , Sondas de ADN , Desoxirribonucleasa HindIII , Femenino , Hibridación Fluorescente in Situ , Masculino , Filogenia , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
Comparative genomic hybridization (CGH) was used to identify and probe sex chromosomes in several XY and WZ systems. Chromosomes were hybridized simultaneously with FluorX-labelled DNA of females and Cy3-labelled DNA of males in the presence of an excess of Cot-1 DNA or unlabelled DNA of the homogametic sex. CGH visualized the molecular differentiation of the X and Y in the house mouse, Mus musculus, and in Drosophila melanogaster: while autosomes were stained equally by both probes, the X and Y chromosomes were stained preferentially by the female-derived or the male-derived probe, respectively. There was no differential staining of the X and Y chromosomes in the fly Megaselia scalaris, indicating an early stage of sex chromosome differentiation in this species. In the human and the house mouse, labelled DNA of males in the presence of unlabelled DNA of females was sufficient to highlight Y chromosomes in mitosis and interphase. In WZ sex chromosome systems, the silkworm Bombyx mori, the flour moth Ephestia kuehniella, and the wax moth Galleria mellonella, the W chromosomes were identified by CGH in mitosis and meiosis. They were conspicuously stained by both female- and male-derived probes, unlike the Z chromosomes, which were preferentially stained by the male-derived probe in E. kuehniella only but were otherwise inconspicuous. The ratio of female:male staining and the pattern of staining along the W chromosomes was species specific. CGH shows that W chromosomes in these species are molecularly well differentiated from the Z chromosomes. The conspicuous binding of the male-derived probe to the W chromosomes is presumably due to an accumulation of common interspersed repetitive sequences.
Asunto(s)
Cromosomas Sexuales , Animales , Drosophila melanogaster/genética , Femenino , Humanos , Masculino , Ratones , Hibridación de Ácido Nucleico , Especificidad de la EspecieRESUMEN
Satellite DNAs (stDNAs) of four Acomys species (spiny-mice), A. cahirinus, A. cineraceus, A. dimidiatus and A. russatus, belong to closely related sequence families. Monomer sizes range from 338 to 364 bp. Between-species sequence identity was from 81.0% to 97.2%. The molecular phylogeny of the sequences helps to clarify the taxonomy of this 'difficult' group. The A. dimidiatus genome contains about 60000 repeats. According to the restriction patterns, repeats are arranged in tandem. The stDNA maps to the centromeric heterochromatin of most autosomes, both acrocentric and metacentric, but appears to be absent in the centromeric region of Y chromosomes. A well-conserved centromere protein B (CENP-B) box is present in the stDNA of A. russatus while it is degenerated in the other species.
Asunto(s)
ADN Satélite , Muridae/clasificación , Muridae/genética , Animales , Secuencia de Bases , Clonación Molecular , Humanos , Cariotipificación , Datos de Secuencia Molecular , Filogenia , Secuencias Repetidas en TándemRESUMEN
The polymorphic Sp100-rs repeat cluster in chromosome band 1D of the house mouse, Mus musculus, makes up as much as 0.1-5% of the haploid genome. 'High-copy' versions of this long-range repeat cluster are cytogenetically apparent as DAPI-negative chromomycin-A3-positive homogeneously staining regions (HSRs). The cluster is a relatively recent acquisition in the genus Mus; the related species M. caroli possesses neither the Sp100-rs cluster nor even the Sp100-rs gene. Except for chromosomes with high-copy clusters, no major rearrangements are visible in chromosomes 1 from M. musculus and M. caroli: they have the same order of G-bands, DAPI-bands and chromomycin A3-bands. Comparative genomic hybridization (CGH) visualizes the cluster in M. musculus and detects a single region of sequence homology to the cluster in M. caroli chromosome band 1D. This indicates that the M. musculus cluster has evolved in situ from sequences originally present in the same chromosome band.
Asunto(s)
Cromosomas , Ratones/genética , Animales , ADN/genética , Familia de Multigenes , Hibridación de Ácido Nucleico , Origen de Réplica , Especificidad de la EspecieRESUMEN
The house mouse, Mus musculus, harbours a variable cluster of long-range repeats in chromosome 1. As shown in previous studies, some high-copy clusters such as the MUT cluster are cytogenetically apparent as a homogeneously staining region (HSR) and are associated with a distortion of the Mendelian recovery ratio when transmitted by heterozygous females. The effect is caused by a decreased viability of +/+ embryos. It is compensated by maternal or paternal MUT. In this study, a deletion derivative of MUT, MUTdel, shows normal transmission ratios and no compensating capability. In this respect, MUTdel behaves like a wild-type cluster. Hence, both properties--transmission ratio distortion and compensating capability--map to the deleted region. The deletion comprises three-quarters of the MUT HSR and does not extend to the nearest markers adjacent to the HSR.
Asunto(s)
Deleción Cromosómica , Ratones/genética , Animales , Mapeo Cromosómico , Femenino , Impresión Genómica , Heterocigoto , HomocigotoRESUMEN
Sp100 is a single-copy gene in the human and the mouse. A related gene, Sp100-rs, occurs in multiple copies and forms a conspicuous cluster in the mouse chromosome 1. Murine Sp100 and Sp100-rs are homologous from the promoter up to a position in intron 3, but they differ 3' of that position. In the genus Mus, Sp100-rs is present in one phylogenetic branch, represented by the house mouse, M. musculus, but probably does not exist in another branch, represented by M. caroli. Thus, Sp100-rs arose relatively late in the evolution of the genus Mus, whereas Sp100 existed in the common ancestor of the human and the mouse. The Sp100-rs gene cluster probably evolved by gene fusion followed by amplification and diversification.
Asunto(s)
Antígenos Nucleares , Autoantígenos/genética , Evolución Molecular , Familia de Multigenes , Proteínas Nucleares/genética , Animales , Fusión Artificial Génica , Secuencia de Bases , Secuencia Conservada , ADN Complementario , Amplificación de Genes , Dosificación de Gen , Intrones , Ratones , Ratones Endogámicos C57BL , Datos de Secuencia Molecular , Homología de Secuencia de Ácido NucleicoRESUMEN
The sex chromosomes of the Megaselia scalaris wild-type strain Wien are homomorphic. We studied a roughly 1.8 kb X/Y DNA segment of this strain. It includes, at one end, the first part of a coding sequence for a protein of the vespid antigen 5 family. Molecular differentiation between the X and Y chromosomes has commenced, but homology, even of short DNA stretches, is still assessable beyond doubt. The most conspicuous differences between the X and the homologous Y segment were insertions/deletions in the noncoding region: among them, deletions, a duplication, and an insertion of a mobile element. These structural changes grossly disrupted homology. In comparison, base substitutions, though more numerous, contributed little to the differentiation of the X/Y DNA segment.
Asunto(s)
Dípteros/genética , Procesos de Determinación del Sexo , Cromosoma X , Cromosoma Y , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , Femenino , Masculino , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Polimorfismo de Longitud del Fragmento de Restricción , Alineación de SecuenciaRESUMEN
We review the synaptonemal complex, SC, of the synapsed homologous chromosomes at meiotic prophase in insects and mammals in terms of its formation, and the association of specific chromatin elements with the synaptonemal complexes. The focus is: (1) The SC as visualized with a variety of techniques; (2) The nature of the chromatin loops where they are associated with the SCs--the bases of the loops may be instrumental in recombinant events judging from the presence of Rad51 protein and late recombination nodules at the SCs; (3) Differences in DNA content of similarly sized loops; (4) Requirements for chromatin attachment to the chromosome cores, requirements that are apparently lacking in foreign DNA inserts; (5) Regulation of loop size by the position along the chromosome; (6) The structural correlates of recombination at the SCs--these comments are based on studies of SC structure, DNA-core protein associations, fluorescent in situ hybridization to visualize specific DNA segments, and fluorescent immunocytology to visualize the chromosome core proteins.