RESUMEN
The UVA (320-380 nm) component of sunlight has oxidizing properties which may be deleterious to skin cells and tissue but can also lead to the strong up-regulation of the heme-catabolizing enzyme, heme oxygenase-1. This enzyme has well-established antioxidant actions in cells as well as anti-inflammatory properties in mammals. There is also evidence from rodent models that this enzyme is responsible for the UVA-mediated protection against UVB-induced immunosuppression that occurs in skin. The relevance of these findings to acute and chronic effects of sunlight including skin carcinogenesis is currently under investigation as are the potential implications for sunlight protection in humans.
Asunto(s)
Hemo-Oxigenasa 1/inmunología , Estrés Oxidativo/inmunología , Enfermedades de la Piel/inmunología , Enfermedades de la Piel/prevención & control , Piel/inmunología , Piel/efectos de la radiación , Rayos Ultravioleta , Animales , Humanos , Terapia de Inmunosupresión/métodos , Ratones , Modelos Animales , Estrés Oxidativo/efectos de la radiación , Dosis de RadiaciónRESUMEN
Ultraviolet A (UVA: 320-380 nm) radiation is an oxidizing carcinogen that has proved an ideal agent for demonstrating the oxidant inducibility of the mammalian heme oxygenase-1 (HO-1) gene. The UVA response in cultured human skin fibroblasts and other cell types is mediated by singlet oxygen and is strongly influenced by cellular reducing equivalents. Free heme, an entity that can be generated by UVA irradiation of cells, also appears to be a critical intermediate that can directly influence both the transcriptional activation and repression of the HO-1 gene. Heme release is likely to be of central importance to the inflammatory response in skin and its abrogation by HO.
Asunto(s)
Carcinógenos , Inducción Enzimática , Hemo Oxigenasa (Desciclizante)/metabolismo , Piel/efectos de la radiación , Luz Solar/efectos adversos , Células Cultivadas , Fibroblastos/citología , Fibroblastos/metabolismo , Fibroblastos/efectos de la radiación , Hemo/metabolismo , Hemo Oxigenasa (Desciclizante)/genética , Hemo-Oxigenasa 1 , Humanos , Proteínas de la Membrana , Oxidantes/metabolismo , Oxígeno/metabolismo , Piel/citología , Piel/patología , Rayos UltravioletaRESUMEN
Tea is rich in antioxidant polyphenols (catechins, flavonols, theaflavins and thearubigins). Epidemiological evidence relating regular consumption of tea or related polyphenols to CHD is equivocal. Catechins are absorbed from tea, but low plasma concentrations are attained. The bioavailability of theaflavins and thearubigins is unknown. Tea does not reduce blood pressure or plasma lipids in well-controlled human trials. Tea polyphenols inhibit LDL lipid peroxidation in vitro, but the effect ex vivo is small. The plasma antioxidant potential increases after drinking green but not black tea. Tea consumption tended to reduce the development of aortic atherosclerosis in rabbits. Tea polyphenols exert marked effects on cells, and inhibit neutrophil migration and inflammatory responses, sometimes at low concentrations. These diverging results suggest potential beneficial effects, but emphasize the need for good human trials of tea using early markers of CHD before firm conclusions can be drawn.
Asunto(s)
Enfermedades Cardiovasculares/prevención & control , Flavonoides/farmacología , Té/química , Animales , Disponibilidad Biológica , Movimiento Celular/efectos de los fármacos , Ensayos Clínicos Controlados como Asunto , Femenino , Flavonoides/química , Humanos , Neutrófilos/efectos de los fármacos , Conejos , RatasRESUMEN
There is considerable current interest in the cytoprotective effects of natural antioxidants against oxidative stress. In particular, epicatechin, a major member of the flavanol family of polyphenols with powerful antioxidant properties in vitro, has been investigated to determine its ability to attenuate oxidative-stress-induced cell damage and to understand the mechanism of its protective action. We have induced oxidative stress in cultured human fibroblasts using hydrogen peroxide and examined the cellular responses in the form of mitochondrial function, cell-membrane damage, annexin-V binding and caspase-3 activation. Since one of the major metabolites of epicatechin in vivo is 3'-O-methyl epicatechin, we have compared its protective effects with that of epicatechin. The results provide the first evidence that 3'-O-methyl epicatechin inhibits cell death induced by hydrogen peroxide and that the mechanism involves suppression of caspase-3 activity as a marker for apoptosis. Furthermore, the protection elicited by 3'-O-methyl epicatechin is not significantly different from that of epicatechin, suggesting that hydrogen-donating antioxidant activity is not the primary mechanism of protection.
Asunto(s)
Antioxidantes/farmacología , Apoptosis/efectos de los fármacos , Caspasas/metabolismo , Catequina/farmacología , Fibroblastos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Caspasa 3 , Catequina/análogos & derivados , Células Cultivadas , Fibroblastos/metabolismo , Humanos , Peróxido de Hidrógeno/farmacología , Lipoproteínas LDL/farmacología , Metilación , Mitocondrias/efectos de los fármacosRESUMEN
This unit presents a method to calculate heme oxygenase enzymatic activity from the formation of bilirubin equivalents [biliverdin-Ix alpha (BV) and bilirubin-IX alpha (BR)]. The BV and BR generated in the reaction are separated by reversed-phase HPLC and detected using visible absorbance spectroscopy. Since both metabolites of heme degradation are directly quantifiable, the assay eliminates the requirement for biliverdin reductase supplementation.
Asunto(s)
Bioensayo/métodos , Cromatografía Líquida de Alta Presión/métodos , Hemo Oxigenasa (Desciclizante)/metabolismo , Hemo/metabolismo , Animales , Bilirrubina/análisis , Biliverdina/análisis , Bioensayo/instrumentación , Catálisis , Células Cultivadas , Cromatografía Líquida de Alta Presión/instrumentación , Humanos , Microsomas/enzimología , Microsomas/metabolismo , Oxidación-ReducciónAsunto(s)
Sistema Inmunológico/efectos de la radiación , Rayos Ultravioleta/efectos adversos , Animales , Hemo Oxigenasa (Desciclizante)/metabolismo , Hemo Oxigenasa (Desciclizante)/efectos de la radiación , Interferón gamma/deficiencia , Interferón gamma/genética , Ratones , Ratones Pelados , Ratones Endogámicos C57BL , Ratones Mutantes , Neoplasias Inducidas por Radiación/etiología , Neoplasias Inducidas por Radiación/metabolismo , Piel/enzimología , Piel/efectos de la radiación , Neoplasias Cutáneas/etiología , Neoplasias Cutáneas/metabolismoAsunto(s)
Oxígeno/fisiología , Rayos Ultravioleta , Animales , Quelantes/farmacología , Óxido de Deuterio/química , Óxido de Deuterio/metabolismo , Hemina/farmacología , Histidina/farmacología , Hierro/análisis , Hierro/fisiología , Proteínas Reguladoras del Hierro , Proteínas Hierro-Azufre/análisis , Proteínas Hierro-Azufre/metabolismo , Oxígeno/antagonistas & inhibidores , Oxígeno/química , Fotoquímica , Proteínas de Unión al ARN/análisis , Proteínas de Unión al ARN/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Oxígeno Singlete , Azida Sódica/farmacologíaRESUMEN
Heme oxygenase (HO) breaks down heme to iron, biliverdin, and carbon monoxide, and activity of this enzyme increases in many tissues and cell types after exposure to oxidative stress. There is evidence that increased HO activity is involved in long-term protective mechanisms against oxidative stress. We studied the effect of artificially overexpressed HO activity on the cytotoxicity of oxidative ultraviolet A (UVA) radiation after loading human cells with the HO substrate ferric heme (hemin). In contrast to the reported long-term protection attributed to HO activity, cells overexpressing HO activity were hypersensitive to UVA radiation shortly after heme treatment when compared with control cells. Cells overexpressing HO activity showed an increased rate of heme consumption and a higher level of accumulated free chelatable iron when compared with control cells. The hypersensitivity of cells overexpressing HO to UVA radiation after heme treatment was apparently caused by the increased accumulation of chelatable iron, because the iron chelator desferrioxamine strongly reduced the hypersensitivity. One day after the heme treatment, cells overexpressing HO activity were no longer hypersensitive to UVA radiation. We conclude that increased HO activity can temporarily increase the sensitivity of cells to oxidative stress by releasing iron from heme.