RESUMEN
Platelet-activating factor (PAF) is a potent phospholipid modulator of inflammation that has diverse physiological and pathological functions. Previously, we demonstrated that PAF has an essential role in ultraviolet (UV)-induced immunosuppression and reduces the repair of damaged DNA, suggesting that UV-induced PAF is contributing to skin cancer initiation by inducing immune suppression and also affecting a proper DNA damage response. The exact role of PAF in modulating cell proliferation, differentiation or transformation is unclear. Here, we investigated the mechanism(s) by which PAF affects the cell cycle and impairs early DNA damage response. PAF arrests proliferation in transformed and nontransformed human mast cells by reducing the expression of cyclin-B1 and promoting the expression of p21. PAF-treated cells show a dose-dependent cell cycle arrest mainly at G2-M, and a decrease in the DNA damage response elements MCPH1/BRIT-1 and ataxia telangiectasia and rad related (ATR). In addition, PAF disrupts the localization of p-ataxia telangiectasia mutated (p-ATM), and phosphorylated-ataxia telangiectasia and rad related (p-ATR) at the site of DNA damage. Whereas the potent effect on cell cycle arrest may imply a tumor suppressor activity for PAF, the impairment of proper DNA damage response might implicate PAF as a tumor promoter. The outcome of these diverse effects may be dependent on specific cues in the microenvironment.
Asunto(s)
Daño del ADN/efectos de los fármacos , Mastocitos/efectos de los fármacos , Mastocitos/fisiología , Factor de Activación Plaquetaria/farmacología , Puntos de Control del Ciclo Celular/efectos de los fármacos , Puntos de Control del Ciclo Celular/fisiología , Línea Celular , Proliferación Celular/efectos de los fármacos , Proliferación Celular/fisiología , Humanos , Mastocitos/citología , Mastocitos/metabolismo , Fosforilación , Factor de Activación Plaquetaria/análogos & derivadosRESUMEN
The First International Symposium on Photopheresis in Hematopoietic Stem Cell Transplantation was held in Vienna, Austria with an educational grant from Therakos Inc. from 25 May to 27 May 2005. Three general issues were addressed: (1) pathophysiology of graft-versus-host disease (GvHD), (2) induction of immune tolerance and the immunology of phototherapy and (3) current standard treatment and prevention strategies of acute and chronic GvHD and the use of extracorporeal photopheresis (ECP). The objectives of the meeting were to open a dialogue among leading researchers in photobiology, immunology, and hematopoietic stem cell transplantation; foster discussions and suggestions for future studies of the mechanism of action of ECP in acute and chronic GvHD; and promote collaboration between basic scientists and clinicians. As can be seen from the summaries of the individual presentations, important advances have been made in our understanding of GvHD, including the use of photoimmunology interventions and the development of robust model systems. It is our expectation that data from photoimmunology studies can be used to generate hypotheses in animal models that can further define the mechanism of action of ECP and help translate the findings to clinical trials of ECP for the prophylaxis and treatment of both chronic and acute GvHD.
Asunto(s)
Enfermedad Injerto contra Huésped/prevención & control , Trasplante de Células Madre Hematopoyéticas/métodos , Fotoféresis , Enfermedad Injerto contra Huésped/etiología , Enfermedad Injerto contra Huésped/inmunología , Enfermedad Injerto contra Huésped/terapia , Humanos , Sistema Inmunológico , Tolerancia Inmunológica , Fotoféresis/métodosRESUMEN
Malting quality has long been an active objective in barley (Hordeum vulgare L.) breeding programs.However, it is difficult for breeders to manipulate malting-quality traits because of inheritance complexity and difficulty in evaluation of these quantitative traits. Quantitative trait locus (QTL) mapping provides breeders a promising basis with which to manipulate quantitative trait genes. A malting-quality QTL complex, QTL2, was mapped previously to a 30-cM interval in the short-arm telomere region of barley chromosome 4H in a "Step-toe"/"Morex" doubled haploid population by the North American Barley Genome Project, using an interval mapping method with a relatively low-resolution genetic map. The QTL2 complex has moderate effects on several malting-quality traits, including malt extract percentage(ME), a-amylase activity (AA), diastatic power (DP), malt 13-glucan content (BG), and seed dormancy, which makes it a promising candidate gene source in malting barley-cultivar development. Fine mapping QTL2 is desirable for precisely studying barley malting-quality trait inheritance and for efficiently manipulating QTL2 in breeding. A reciprocal-substitution mapping method was employed to fine map QTL2. Molecular marker-assisted backcrossing was used to facilitate the generation of isolines. Fourteen different types of "Steptoe" isolines, including regenerated "Steptoe" and 13 different types of "Morex" isolines,including regenerated "Morex", were made within a 41.5-cM interval between MWG634 and BCD265B on chromosome 4H. Duplicates were identified for 12 "Steptoe" and 12 "Morex" isoline types. The isolines together with "Steptoe" and "Morex" were grown variously at three locations in 2 years for a total of five field environments.Four malting-quality traits were measured: ME, DP, AA,and BG. Few significant differences were found between duplicate isolines for these traits. A total of 15 putative QTLs were mapped; three for ME, four for DP, six for AA,and two for BG. Background genotype seemed to make a difference in expression/detection of QTLs. Of the 15 QTLs identified, ten were from the "Morex" and only five from the "Steptoe" background. By combining the results from different years, field environments, and genetic backgrounds and taking into account overlapping QTLsegments, six QTLs can be conservatively estimated: two each for ME and AA and one each for DP and BG with chromosome segments ranging from 0.7 cM to 27.9 cM. A segment of 15.8 cM from the telomere (MWG634-CDO669) includes all or a portion of all QTLs identified. Further study and marker-assisted breeding should focus on this 15.8-cM chromosome region.
Asunto(s)
Mapeo Cromosómico , Hordeum/genética , Sitios de Carácter Cuantitativo , Cruzamiento/métodos , Cruzamientos Genéticos , FenotipoRESUMEN
The genotoxic potential of the jet fuels, Jet-A and JP-8, were examined in mice treated on the skin with a single dose of 240 mg/mouse. Peripheral blood smears were prepared at the start of the experiment (t = 0), and at 24, 48 and 72 h following treatment with jet fuels. Femoral bone marrow smears were made when all animals were sacrificed at 72 h. In both tissues, the extent of genotoxicity was determined from the incidence of micronuclei (MN) in polychromatic erythrocytes. The frequency of MN in the peripheral blood of mice treated with Jet-A and JP-8 increased over time and reached statistical significance at 72 h, as compared with concurrent control animals. The incidence of MN was also higher in bone marrow cells of mice exposed to Jet-A and JP-8 as compared with controls. Thus, at the dose tested, a small but significant genotoxic effect of jet fuels was observed in the blood and bone marrow cells of mice treated on the skin.
Asunto(s)
Combustibles Fósiles/toxicidad , Hidrocarburos/toxicidad , Administración Cutánea , Animales , Células Sanguíneas/efectos de los fármacos , Células Sanguíneas/ultraestructura , Células de la Médula Ósea/efectos de los fármacos , Células de la Médula Ósea/ultraestructura , Ciclofosfamida/administración & dosificación , Ciclofosfamida/toxicidad , Eritrocitos Anormales/efectos de los fármacos , Femenino , Hidrocarburos/administración & dosificación , Ratones , Ratones Endogámicos C3H , Pruebas de Micronúcleos , Distribución Aleatoria , Método Simple Ciego , Organismos Libres de Patógenos Específicos , Coloración y EtiquetadoRESUMEN
Seed dormancy in barley ( Hordeum vulgare L.) is one of the most important parameters affecting malting. Seed dormancy is quantitatively inherited and variously influenced by the environment. The objectives of the present study were to determine the genome location and effects of quantitative trait loci (QTLs) involved in the expression of seed dormancy in a barley cross between two varieties derived from different germplasm pools. Using a doubled-haploid population of 107 lines of the cross between the malting types Triumph (two-row, dormant) and Morex (six-row, non-dormant), seed dormancy phenotypic data sets from five environments and a 147-marker linkage map were developed in order to perform QTL analyses with simple interval mapping and simplified composite interval mapping procedures. Two different types of variables were considered for seed dormancy characterization: (1) level of dormancy induced during seed development, which was indirectly measured as germination percentage at 3 days and 7 days, GP3 and GP7 respectively; (2) rate of dormancy release in the course of a period after seed harvest (after-ripening). Different mechanisms of genetic control were detected for these two types of dormancy-related traits. A major and consistent dormancy QTL near the centromere on chromosome 7(5H) was associated with the establishment of dormancy during seed development and accounted for 52% and 33% of the variability for GP3 and GP7, respectively. Two other QTLs located in the vicinity of the vrs1 locus on chromosome 2(2H) and near the long arm telomere on chromosome 7(5H) explained 9% and 19% of variation, respectively, for the rate of dormancy release during after-ripening. Likewise, seed dormancy was assessed in an F(2) population derived from the cross between two dormant types of distinct germplasm groups, Triumph (European, two-row, malt) and Steptoe (North American, six-row, feed), which showed similar but not identical genetic control for dormancy. Interestingly, there is remarkable dormancy QTL conservation in both regions on chromosome 7(5H) identified in this study and among other barley mapping populations. These widely conserved QTLs show potential as targets for selection of a moderate level of seed dormancy in breeding programs.
Asunto(s)
Hordeum/genética , Fenotipo , Sitios de Carácter Cuantitativo/genética , Semillas/fisiología , Mapeo Cromosómico , Cruzamientos Genéticos , Hordeum/fisiología , Repeticiones de Minisatélite/genética , Polimorfismo de Longitud del Fragmento de RestricciónRESUMEN
Moderate seed dormancy is desirable in barley (Hordeum vulgare L.). It is difficult for breeders to manipulate seed dormancy in practical breeding programs because of complex inheritance and large environmental effects. Quantitative trait locus (QTL) mapping opens a way for breeders to manipulate quantitative trait genes. A seed dormancy QTL, SD2, was mapped previously in an 8-cM interval near the chromosome 7 (5H) L telomere from a cross of 'Steptoe' (dormant)/'Morex' (non-dormant) by the North American Barley Genome Project using an interval mapping method and a relatively low-resolution genetic map. SD2 has a moderate dormancy effect, which makes it a promising candidate gene for moderate seed dormancy in barley cultivar development. The fine mapping of SD2 is required for efficient manipulation of SD2 in breeding and would facilitate the study of dormancy in barley. Ten different Morex isolines were generated, including regenerated Morex, of which nine lines had duplicates. The isolines together with Steptoe and Morex were grown in growth room and field environments for 2 years (2000 and 2001). In the growth room, relatively low growing temperatures (25 degrees C day/15 degrees C night) were employed to promote seed dormancy development. Seed germination percentage, determined at different post-harvest after-ripening periods, was used to measure seed dormancy. Fine mapping using the substitution mapping method based on differences among isolines resolved the SD2 QTL into an 0.8-cM interval between molecular markers MWG851D and MWG851B near the chromosome 7 (5H) L telomere. Relatively low temperatures (< or =25 degrees C) during seed development promoted the expression of the SD2 dormancy QTL. The chromosome region above the MWG851D-MWG851B interval might play a role in reducing barley seed dormancy during after-ripening.
Asunto(s)
Mapeo Cromosómico , Hordeum/genética , Sitios de Carácter Cuantitativo/genética , Semillas/fisiología , Germinación/fisiología , Hordeum/fisiología , TemperaturaRESUMEN
A combination of 8-methoxypsoralen (8-MOP) and ultraviolet-A (UVA) radiation (320-400 nm) (PUVA) is widely used in the treatment of psoriasis and other skin diseases. PUVA is highly effective in eliminating hyperproliferative cells in the epidermis, but its mechanism of action has not been fully elucidated. In this study, we used immortalized JB6 mouse epidermal cells, p53(-/-), and Fas ligand deficient (gld) mice to investigate the molecular mechanism by which PUVA induces cell death. The results indicate that PUVA treatment induces apoptosis in JB6 cells. In addition, PUVA treatment of JB6 cells results in p53 stabilization, phosphorylation, and nuclear localization as well as induction of p21(Waf/Cip1) and caspase-3 activity. In vivo studies reveal that PUVA treatment induces significantly less apoptosis in the epidermis of p53(-/-) mice compared to p53(+/+) mice. Furthermore, FasL-deficient (gld) mice are completely resistant to PUVA-induced apoptosis compared to wild-type mice. These results indicate that PUVA treatment induces apoptosis in mouse epidermal cells in vitro and in vivo and that p53 and Fas/Fas ligand interactions are required for this process, at least in vivo. This implies that similar mechanisms may be involved in the elimination of psoriatic keratinocytes from human skin following PUVA therapy.
Asunto(s)
Apoptosis/fisiología , Epidermis/efectos de la radiación , Glicoproteínas de Membrana/metabolismo , Metoxaleno/farmacología , Proteína p53 Supresora de Tumor/metabolismo , Rayos Ultravioleta , Transporte Activo de Núcleo Celular/efectos de los fármacos , Transporte Activo de Núcleo Celular/efectos de la radiación , Animales , Animales Modificados Genéticamente , Caspasa 3 , Caspasas/metabolismo , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/efectos de la radiación , Células Cultivadas , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Ciclinas/biosíntesis , Activación Enzimática , Epidermis/efectos de los fármacos , Epidermis/fisiología , Proteína Ligando Fas , Glicoproteínas de Membrana/deficiencia , Glicoproteínas de Membrana/genética , Ratones , Ratones Endogámicos BALB C , Receptor fas/genética , Receptor fas/metabolismoRESUMEN
The ultraviolet radiation present in sunlight is the primary cause of nonmelanoma skin cancer and has been implicated in the development of cutaneous malignant melanoma. In addition, ultraviolet is immune suppressive and the suppression induced by ultraviolet radiation has been identified as a risk factor for skin cancer induction. Ultraviolet also suppresses the immune response to infectious agents. In most experimental models, ultraviolet is applied to immunologically naive animals prior to immunization. Of equal concern, however, is the ability of sunlight to suppress established immune reactions, such as the recall reaction in humans, which protects against microbial infections. Here we demonstrate that solar-simulated ultraviolet radiation, applied after immunization, suppresses immunologic memory and the elicitation of delayed-type hypersensitivity. Further, we found that wavelengths in the ultraviolet A region of the solar spectrum were critical for inducing immune suppression. Ultraviolet A (320-400 nm) radiation was as effective as solar-simulated ultraviolet A + B (290-400 nm) in suppressing the elicitation of an established immune response. Irradiation with ultraviolet AI (340-400 nm) had no effect. Supporting a critical role for ultraviolet A in ultraviolet-induced immune suppression was the observation that applying a sunscreen that contained an ultraviolet B only filter had no protective effect, whereas, a sunscreen containing both ultraviolet A and ultraviolet B filters totally blocked ultraviolet-induced immune suppression. These data suggest that sunlight may depress the protective effect of prior vaccination. In addition, the observation that ultraviolet A is immunosuppressive indicates the need for ultraviolet A protection when designing sun protection strategies.
Asunto(s)
Sistema Inmunológico/efectos de la radiación , Rayos Ultravioleta , Animales , Formación de Anticuerpos/efectos de los fármacos , Formación de Anticuerpos/efectos de la radiación , Química Farmacéutica , Hipersensibilidad Tardía/prevención & control , Sistema Inmunológico/efectos de los fármacos , Inmunización , Memoria Inmunológica/efectos de la radiación , Ratones , Ratones Endogámicos C3H , Protectores Solares/química , Protectores Solares/farmacologíaRESUMEN
Epidemiological studies have suggested an association between exposure to solar UV radiation and the incidence of lymphoid malignancies, which has increased substantially worldwide during the last two decades. Findings from animal studies have raised the question of whether UV radiation might influence the development of lymphoid malignancies by means of its immunosuppressive effect. In this study, we examined the effect of UV irradiation on the development of lymphoid malignancies in mice with no or only one functional copy of p53. Mice that lack both copies of p53 spontaneously develop high frequency of lymphoid malignancies in the thymus and spleen. p53 heterozygous mice with only one copy of the wild-type allele also develop lymphoid malignancies, but with a much lower frequency and a long latent period. In our study using mice of the C57BL/6 background, only one of the unirradiated mice lacking one copy of p53 (p53(+/-)) spontaneously developed a lymphoid tumor (6%), whereas 88% of UV-irradiated p53(+/-) mice developed lymphoid tumors in the spleen or liver. None of the control or UV-irradiated p53 wild-type mice developed lymphoid tumors during the 60-week observation period. Both UV-irradiated and unirradiated mice lacking both copies of p53 (p53(-/-)) rapidly developed thymic lymphomas and/or lymphoid tumors in spleen or liver. All of the lymphoid tumors tested were of T cell type. The immune responses of the mice to contact sensitization were identical and were suppressed to the same extent by UV irradiation regardless of the genotype. These results indicate that differences in immune reactivity do not account for the different effects of UV radiation on lymphoid malignancies and, in addition, that p53 is not required for generation of T cell-mediated immunity. Interestingly, whereas p53 mutations or loss of heterozygosity did not account for the accelerated development of lymphoid tumors in UV-irradiated p53(+/-) mice, deletions in the p16(INK4a) gene were quite common. These data provide the experimental evidence that UV irradiation induces lymphoid neoplasms in genetically susceptible mice and support the hypothesis that extensive sunlight exposure contributes to the induction of lymphoma in humans.
Asunto(s)
Eliminación de Gen , Genes p53 , Linfoma no Hodgkin/etiología , Neoplasias Inducidas por Radiación/genética , Rayos Ultravioleta/efectos adversos , Factores de Edad , Animales , Animales Congénicos , Genes p16/efectos de la radiación , Hipersensibilidad Tardía/genética , Hipersensibilidad Tardía/inmunología , Inmunidad Celular/genética , Inmunidad Celular/efectos de la radiación , Neoplasias Hepáticas/etiología , Neoplasias Hepáticas/genética , Linfoma no Hodgkin/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Organismos Libres de Patógenos Específicos , Neoplasias del Bazo/etiología , Neoplasias del Bazo/genética , Neoplasias del Timo/etiología , Neoplasias del Timo/genéticaRESUMEN
These studies addressed the hypothesis that UV radiation (UVR) could affect immune responses in mice infected with Borrelia burgdorferi. Immunity against the Lyme spirochete B. burgdorferi was studied in a murine model of UV-induced immune suppression. Borrelia-specific cellular and humoral responses were examined following immunosuppressive doses of UVR. Low-passage Borrelia were injected intradermally at the base of the tail following irradiation. At various time points after infection the blood was cultured for the presence of Borrelia and the serum analyzed for Borrelia-specific antibodies. Two weeks after infection one hind-limb joint was cultured for the presence of spirochetes and the contralateral joint was examined histologically for arthritis formation. The results demonstrated that UV irradiation, administered at the site of infection or at a distant site, suppressed Borrelia-specific cellular and humoral responses in infected mice. Suppression of delayed-type hypersensitivity and antibody responses to UV was abrogated by administration of anti-interleukin (IL)-10 after UV irradiation. In addition, UV irradiation altered the dissemination pattern of the bacteria from the skin into the blood and exacerbated arthritis when compared with unirradiated controls. From these studies we concluded that UV irradiation can modulate the immune response to Borrelia and exacerbate the subsequent arthritic component of Lyme disease in mice. Furthermore, our studies suggest that IL-10 is in part responsible for the suppression of both cellular and humoral responses in addition to playing a role in the development of Lyme arthritis.
Asunto(s)
Grupo Borrelia Burgdorferi/inmunología , Enfermedad de Lyme/inmunología , Rayos Ultravioleta , Traslado Adoptivo , Animales , Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Antibacterianos/inmunología , Femenino , Hipersensibilidad Tardía/inmunología , Hipersensibilidad Tardía/microbiología , Interleucina-10/inmunología , Ratones , Ratones Endogámicos C3H , Células TH1/inmunologíaRESUMEN
For nearly 100 yr physicians and scientists have appreciated the carcinogenic potential of the ultraviolet (UV) radiation present in sunlight 0(1,2). During the latter part of the twentieth century, immunologists and dermatologists realized that UV radiation suppressed the immune response (3-5). Moreover, the immune suppression induced by UV radiation is a major risk factor for the induction of nonmelanoma skin cancer (6). The association between nonmelanoma skin cancer induction and immune suppression has fueled the efforts of many to study the immunologic mechanism underlying UV-induced immune suppression. This chapter focuses on describing the methods used to dissect the suppressive effects of UV on the immune system, concentrating particularly on in vivo models of immunity.
RESUMEN
Dermal application of JP-8 jet fuel induces immune suppression. Classic delayed-type hypersensitivity as well as the induction of contact hypersensitivity to allergens applied to the shaved skin of JP-8-treated mice is suppressed. In addition, the ability of T cells isolated from JP-8-treated mice to proliferate in vitro is suppressed. Here we focused on further characterizing the immunotoxicity induced by JP-8 exposure and determining the mechanism involved. Suppression of T-cell proliferation was first noted 3 to 4 days after a single JP-8 treatment and lasted for approximately 3 weeks, at which time T-cell proliferation returned to normal. Cellular immune reactions appear to be more susceptible to the immunosuppressive effects of JP-8, as antibody production in JP-8-treated mice was identical to that found in normal controls. The mechanism through which dermal application of JP-8 suppresses cell-mediated immune reactions appears to be via the release of immune biological-response modifiers. Blocking the production of prostaglandin E(2) with a selective cyclooxygenase-2 inhibitor abrogated JP-8-induced immune suppression. Neutralizing the activity of interleukin-10 with a highly specific monoclonal antibody also blocked JP-8-induced immune suppression. Furthermore, injecting JP-8-treated mice with recombinant interleukin-12, a cytokine that drives cell-mediated immune reactions in vivo, overcame the immunotoxic effects of JP-8 and restored immune function. These data indicate that immune suppressive cytokines, presumably produced by JP-8-treated epidermal cells, are responsible for immune suppression in JP-8-treated mice and that blocking and/or neutralizing their production in vivo overcomes the immunotoxic effects of JP-8.
Asunto(s)
Hidrocarburos/toxicidad , Tolerancia Inmunológica/efectos de los fármacos , Queroseno/toxicidad , Administración Cutánea , Animales , Formación de Anticuerpos , Inhibidores de la Ciclooxigenasa/farmacología , Dinoprostona/fisiología , Femenino , Hidrocarburos/administración & dosificación , Interleucina-10/fisiología , Interleucina-12/farmacología , Ratones , Ratones Endogámicos C3H , Proteínas Recombinantes/farmacologíaRESUMEN
UV-induced immune suppression is a risk factor for sunlight-induced skin cancer. Exposure to UV radiation has been shown to suppress the rejection of highly antigenic UV-induced skin cancers, suppresses delayed and contact hypersensitivity, and depress the ability of dendritic cells to present Ag to T cells. One consequence of UV exposure is altered activation of T cell subsets. APCs from UV-irradiated mice fail to present Ag to Th1 T cells; however, Ag presentation to Th2 T cells is normal. While this has been known for some time, the mechanism behind the preferential suppression of Th1 cell activation has yet to be explained. We tested the hypothesis that this selective impairment of APC function results from altered cytokine production. We found that dendritic cells/macrophages (DC/Mphi) from UV-irradiated mice failed to secrete biologically active IL-12 following in vitro stimulation with LPS. Instead, DC/Mphi isolated from the lymphoid organs of UV-irradiated mice secreted IL-12p40 homodimer, a natural antagonist of biologically active IL-12. Furthermore, when culture supernatants from UV-derived DC/Mphi were added to IL-12-activated T cells, IFN-gamma secretion was totally suppressed, indicating that the IL-12p40 homodimer found in the supernatant fluid was biologically active. We suggest that by suppressing DC/Mphi IL-12p70 secretion while promoting IL-12p40 homodimer secretion, UV exposure preferentially suppress the activation of Th1 cells, thereby suppressing Th-1 cell-driven inflammatory immune reactions.
Asunto(s)
Células Dendríticas/metabolismo , Células Dendríticas/efectos de la radiación , Inmunosupresores/efectos de la radiación , Interleucina-12/metabolismo , Interleucina-12/efectos de la radiación , Macrófagos/metabolismo , Macrófagos/efectos de la radiación , Rayos Ultravioleta , Animales , Sistema Libre de Células , Células Dendríticas/inmunología , Dimerización , Ensayo de Inmunoadsorción Enzimática , Femenino , Separación Inmunomagnética , Inmunosupresores/aislamiento & purificación , Inmunosupresores/metabolismo , Inmunosupresores/farmacología , Interleucina-12/aislamiento & purificación , Interleucina-12/fisiología , Macrófagos/inmunología , Ratones , Ratones Endogámicos C3HRESUMEN
Exposure to ultraviolet (UV) radiation, a complete carcinogen, suppresses the immune response. Data from a number of laboratories have indicated that one consequence of UV exposure is suppressed T helper type 1 (Th1) cell function with normal Th2 cell activation, resulting in a shift to a Th2-like phenotype. The reversal of UV-induced immune suppression and tolerance induction by recombinant interleukin-12 (rIL-12) supports this observation. The focus of this study was to determine the mechanism(s) by which rIL-12 reverses UV-induced immune suppression. Two possibilities were considered: up-regulation of interferon-gamma (IFN-gamma) secretion by rIL-12 and suppression of UV-induced cytokine secretion by rIL-12. To our surprise we found that the ability of rIL-12 to overcome UV-induced immune suppression was independent of its ability to up-regulate IFN-gamma secretion. Rather, rIL-12 suppressed the production of cytokines that are known to be important in UV-induced immune suppression. Injecting UV-irradiated mice with rIL-12, or adding rIL-12 to UV-irradiated keratinocyte cultures suppressed IL-10 secretion, in part by affecting the transcription of the IL-10 gene. Furthermore, we found that rIL-12 suppressed UV-induced tumour necrosis factor-alpha (TNF-alpha) production. Because IL-10 is involved in the UV-induced suppression of delayed-type hypersensitivity and TNF-alpha in the UV-induced suppression of contact allergy, these findings provide a mechanism to explain how rIL-12 overcomes UV-induced immune suppression in these related but different immune reactions. In addition, they suggest a novel mechanism by which rIL-12 alters immune reactivity, direct suppression of cytokine secretion induced by UV radiation.
Asunto(s)
Citocinas/biosíntesis , Tolerancia Inmunológica/efectos de la radiación , Interleucina-12/inmunología , Rayos Ultravioleta , Animales , Línea Celular , Femenino , Tolerancia Inmunológica/inmunología , Interferón gamma/biosíntesis , Interferón gamma/inmunología , Interleucina-10/biosíntesis , Interleucina-10/genética , Queratinocitos/inmunología , Queratinocitos/efectos de la radiación , Ratones , Ratones Endogámicos C3H , Proteínas Recombinantes/inmunología , Transcripción Genética , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Whereas CD40-CD40 ligand interactions are important for various dendritic cell (DC) functions in vitro, their in vivo relevance is unknown. We analyzed the DC status of CD40 ligand -/- mice using a contact hypersensitivity (CHS) model system that enables multiple functions of DCs to be assessed in vivo. Immunohistochemistry of skin sections revealed no differences in terms of numbers and morphology of dendritic epidermal Langerhans cells (LCs) in unsensitized CD40 ligand -/- mice as compared with wild-type C57BL/6 mice. However, after contact sensitization of CD40 ligand -/- mice, LCs failed to migrate out of the skin and substantially fewer DCs accumulated in draining lymph nodes (DLNs). Furthermore, very few antigen-bearing DCs could be detected in the paracortical region of lymph nodes draining sensitized skin. This defect in DC migration after hapten sensitization was associated with defective CHS responses and decreased cutaneous tumor necrosis factor (TNF)-alpha production and was corrected by injecting recombinant TNF-alpha or an agonistic anti-CD40 monoclonal antibody. Thus, CD40-CD40 ligand interactions in vivo regulate the migration of antigen-bearing DCs from the skin to DLNs via TNF-alpha production and play a vital role in the initiation of acquired T cell-mediated immunity.
Asunto(s)
Antígenos CD40/inmunología , Movimiento Celular/inmunología , Células de Langerhans/inmunología , Glicoproteínas de Membrana/inmunología , Animales , Ligando de CD40 , Recuento de Células , Dermatitis por Contacto , Modelos Animales de Enfermedad , Células de Langerhans/clasificación , Células de Langerhans/citología , Células de Langerhans/fisiología , Ganglios Linfáticos , Glicoproteínas de Membrana/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Piel/citología , Factor de Necrosis Tumoral alfa/biosíntesisRESUMEN
Exposure to ultraviolet radiation induces skin cancer. In addition, UV exposure suppresses the immune response. The mechanism by which skin exposure to UV induces systemic immune suppression is not entirely clear, but a role for cytokines secreted by irradiated epidermal cells has been described. Ultimately, these immune regulatory cytokines affect antigen presenting cell function at distant sites. We describe here preliminary findings suggesting that one consequence of UV exposure is an alteration of IL-12 production by lymph node dendritic cells that result in impaired immune function.
Asunto(s)
Citocinas/inmunología , Tolerancia Inmunológica/efectos de la radiación , Rayos Ultravioleta/efectos adversos , Animales , Presentación de Antígeno/efectos de la radiación , Células Dendríticas/inmunología , Células Dendríticas/efectos de la radiación , Humanos , Interleucina-12/biosíntesis , Neoplasias Cutáneas/etiología , Neoplasias Cutáneas/inmunologíaRESUMEN
We examined the effects of ultraviolet (UV) radiation on the time course for induction of sunburn (apoptotic) cells and expression of proteins known to be associated with growth arrest and apoptosis in SKH-hr1 mouse skin. Mice were irradiated with a single dose (2.5 kJ/m(2)) of UV from Kodacel-filtered (290-400 nm) FS40 sunlamps and the skin tissues were analyzed at various times after irradiation for the presence of apoptotic cells and expression of p53, p21(Waf-1/Cip1), bcl-2, bax, and proliferating cell nuclear antigen. The results indicated that p53 expression was induced early in the epidermis, reaching maximum levels 12 hours after irradiaton, and p21(Waf-1/Cip1) expression in the epidermis peaked at 24 hours after irradiation. In contrast, UV radiation induced high levels of bax at 24 to 72 hours after irradiation with a concomitant decrease in bcl-2 expression. Coinciding with these changes, apoptotic cells began to appear 6 hours after irradiation and reached a maximum at 24 hours after irradiation. Interestingly, proliferating cell nuclear antigen expression, which was initially confined to the basal layer, became dispersed throughout the basal and suprabasal layers of the skin at 48 hours and paralleled marked hyperplasia. These results suggest that UV irradiation of mouse skin induces apoptosis mediated by the p53/p21/bax/bcl-2 pathway and that the dead cells are replaced by hyperproliferative cells, leading to epidermal hyperplasia. This implies that UV-induced apoptosis and hyperplasia are closely linked and tightly regulated and that dysregulation of these two events may lead to skin cancer development.
Asunto(s)
Adaptación Fisiológica , Traumatismos Experimentales por Radiación/fisiopatología , Piel/efectos de la radiación , Quemadura Solar/fisiopatología , Rayos Ultravioleta , Animales , Apoptosis , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Ciclinas/metabolismo , Femenino , Hiperplasia , Etiquetado Corte-Fin in Situ , Ratones , Ratones Pelados , Antígeno Nuclear de Célula en Proliferación/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Piel/metabolismo , Piel/patología , Piel/fisiopatología , Quemadura Solar/metabolismo , Quemadura Solar/patología , Factores de Tiempo , Distribución Tisular , Proteína p53 Supresora de Tumor/metabolismo , Proteína X Asociada a bcl-2RESUMEN
Ultraviolet (UV) radiation is carcinogenic and immunosuppressive. UV-induced immune suppression is mediated by antigen-specific T cells, which can transfer suppression to normal recipients. These cells are essential for controlling skin cancer development in the UV-irradiated host and in suppressing other immune responses, such as delayed-type hypersensitivity. Despite their importance in skin cancer development, their exact identity has remained elusive. We show here that natural killer T cells from UV-irradiated donor mice function as suppressor T cells and play a critical role in regulating the growth of UV-induced skin cancers and suppressing adaptive immune responses in vivo.
Asunto(s)
Células Asesinas Naturales/inmunología , Neoplasias Cutáneas/inmunología , Subgrupos de Linfocitos T/inmunología , Animales , Antígenos/administración & dosificación , Antígenos CD1/genética , Antígenos CD1/metabolismo , Femenino , Hipersensibilidad Tardía , Tolerancia Inmunológica , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Ratones Noqueados , Neoplasias Inducidas por Radiación/inmunología , Bazo/inmunología , Rayos Ultravioleta/efectos adversosRESUMEN
We examined dendritic cell (DC) status in SCID and RAG2 -/- mice to assess the influence of T cells on DC development and function in vivo. These mice have reduced numbers of DC in the epidermis and lymph nodes draining hapten-sensitized skin. Epidermal DC in these mice were defective in presenting antigen in vivo to adoptively transferred, hapten-sensitized T cells from normal mice. Likewise, draining lymph node DC were deficient in their capacity to stimulate naive T cells in vitro and in vivo. DC numbers as well as the impaired ability to present antigen in vivo, were corrected by reconstituting these animals with normal T lymphocytes, suggesting that T cells are crucial for normal DC maturation and function in vivo.
Asunto(s)
Células Dendríticas/citología , Subgrupos de Linfocitos T/fisiología , Traslado Adoptivo , Animales , Presentación de Antígeno , Recuento de Células , Diferenciación Celular , Proteínas de Unión al ADN/deficiencia , Proteínas de Unión al ADN/genética , Dermatitis Alérgica por Contacto/inmunología , Dermatitis Alérgica por Contacto/patología , Epidermis/inmunología , Epidermis/patología , Haptenos/inmunología , Células de Langerhans/patología , Ganglios Linfáticos/inmunología , Ganglios Linfáticos/patología , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones SCID , Subgrupos de Linfocitos T/trasplanteRESUMEN
Chronic exposure to JP-8 jet fuel induces lung toxicity, adverse neurological effects and some liver and kidney dysfunction. In addition, inhalation of JP-8 induces immune suppression. Besides the lung, the other major route of JP-8 exposure is via the skin. In this study we tested the hypothesis that dermal exposure to JP-8 is immune suppressive. JP-8 was applied to the skin of adult female C3H/HeN mice and various immune parameters were examined. Dermal exposure to JP-8, either multiple small exposures (50 microl for 5 days) or a single large dose (250-300 microl) resulted in immune suppression. The induction of contact hypersensitivity was impaired in a dose-dependent manner regardless of whether the contact allergen was applied directly to the JP-8-treated skin or at a distant un-treated site. In addition, the generation of a classic delayed-type hypersensitivity reaction to a bacterial antigen (Borellia burgdorferi) injected into the subcutaneous space was suppressed by dermal application of JP-8 at a distant site. The ability of splenic T lymphocytes from JP-8-treated mice to proliferate in response to plate-bound monoclonal anti-CD3 was also significantly suppressed. Interleukin-10, a cytokine with potent immune suppressive activity, was found in the serum of JP-8-treated mice, suggesting that the mechanism of systemic immune suppression may involve the upregulation of cytokine release by JP-8. These findings confirm the immunosuppressive effects of JP-8 and demonstrate that dermal exposure to JP-8 is immunotoxic.