RESUMEN
This study was conducted to investigate the presence of Epstein-Barr virus (EBV) and human papillomavirus (HPV) and the promoter methylation status of the death-associated protein kinase (DAPK) gene in high-grade intraepithelial lesions. Viral infection was analyzed using polymerase chain reaction (PCR), and promoter methylation status was evaluated using chemical modification by sodium bisulfite followed by PCR. A total of 24 samples were studied. HPV was detected in 16.6%, EBV in 16.6%, and HPV/EBV coinfection in 16.6%. No virus infection was detected in 50% of the samples studied. DAPK promoter methylation was observed in 29.2% of the analyzed samples. There was no significant correlation between DAPK methylation and viral infection. DAPK methylation was detected in 28% of HPV-positive lesions, in 28% of HPV- and EBV-positive lesions, and in 44% (3/7) of the samples without viral infection. There was no observed methylation in samples with isolated EBV infection. In DAPK unmethylated samples, HPV infection was found in 12%, EBV infection in 23%, HPV/EBV coinfection in 12%, and an absence of HPV and EBV infection in 53%. The promoter methylation of the DAPK gene is an important event during carcinogenesis and may have potential clinical application as a marker for the progression and prognosis of cancer.
Asunto(s)
Alphapapillomavirus/genética , Proteínas Reguladoras de la Apoptosis/genética , Proteínas Quinasas Dependientes de Calcio-Calmodulina/genética , Carcinoma de Células Escamosas/genética , Metilación de ADN , ADN Viral/análisis , Herpesvirus Humano 4/genética , Displasia del Cuello del Útero/genética , Neoplasias del Cuello Uterino/genética , Alphapapillomavirus/aislamiento & purificación , Secuencia de Bases , Carcinoma de Células Escamosas/epidemiología , Carcinoma de Células Escamosas/patología , Carcinoma de Células Escamosas/virología , Proteínas Quinasas Asociadas a Muerte Celular , Infecciones por Virus de Epstein-Barr/epidemiología , Infecciones por Virus de Epstein-Barr/genética , Infecciones por Virus de Epstein-Barr/virología , Femenino , Células HeLa , Herpesvirus Humano 4/aislamiento & purificación , Humanos , Datos de Secuencia Molecular , Infecciones por Papillomavirus/epidemiología , Infecciones por Papillomavirus/genética , Infecciones por Papillomavirus/virología , Prevalencia , Regiones Promotoras Genéticas , Células Tumorales Cultivadas , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/patología , Neoplasias del Cuello Uterino/virología , Displasia del Cuello del Útero/epidemiología , Displasia del Cuello del Útero/patología , Displasia del Cuello del Útero/virologíaRESUMEN
Os autores apresentam 85 pacientes com I.U.E. operadas pela tecnica proposta por Burch. Apos descrever os criterios adotados no diagnostico da I.U.E., as indicacoes da via retropubica e os cuidados peroperatorios, apresentam suas complicacoes no pos-operatorio imediato ligadas a cirurgia adotada. As possiveis causas que contribuiram sao analisadas e medidas profilaticas sao propostas