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1.
Cancer Genet Cytogenet ; 146(1): 48-57, 2003 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-14499696

RESUMEN

Occurrence of 13q14 deletions between D13S273 and D13S25 in B-cell chronic lymphocytic leukemia (B-CLL) suggests that the region contains a tumor suppressor gene. We constructed a PAC/cosmid contig largely corresponding to a 380-kb 13q14 YAC insert that we found deleted in a high proportion of B-CLL patients. We found seven genes by exon trapping, cDNA screening and analysis/cDNA extension of known expressed sequence tags. One appeared to originate from another region of 13q. Recent publications have focused on two of the genes that most likely do not have a tumor suppressor role. This study evaluates the remaining four genes in the region by mutation scanning and theoretical analysis of putative encoded products. No mutations suggestive of a pathogenic effect were found. The 13q14 deletions may be a consequence of an inherent instability of the region, an idea supported by our finding of a considerable proportion of AluY repeats. Deletion of putative enhancer sequences and/or genes in the region may result in an inactivation of tumor suppression by a haploinsufficiency mechanism. We conclude that RFP2, c13ORF1, and a chromosome 13-specific ST13-like gene, FAM10A4, are the most likely candidates for such a type of B-CLL TSG.


Asunto(s)
Linfocitos B/patología , Genes Supresores de Tumor , Leucemia Linfocítica Crónica de Células B/genética , Mapeo Cromosómico , Cromosomas Humanos Par 13 , Análisis Mutacional de ADN , Etiquetas de Secuencia Expresada , Humanos , Hibridación Fluorescente in Situ , Eliminación de Secuencia
3.
Blood ; 95(11): 3514-9, 2000 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-10828037

RESUMEN

Deletion of the multidrug resistance gene MRP1 has been demonstrated in acute myeloid leukemia (AML) patients with inversion of chromosome 16 (inv[16]). These AML patients are known to have a relatively favorable prognosis, which suggests that MRP1 might play an important role in determining clinical outcome. This study analyzed MRP1 deletion by fluorescent in situ hybridization (FISH), with a focus on inv(16) AML patients. Functional activity of multidrug resistance protein (MRP) was studied in a flow cytometric assay with the use of the MRP substrate carboxyfluorescein (CF) and the inhibitor MK-571. MRP1, MRP2, and MRP6 messenger RNA (mRNA) expression was determined with reverse transcriptase-polymerase chain reaction (RT-PCR). The results were compared with normal bone marrow cells. MRP1 deletion was detected in 7 AML patients; 2 cases showed no MRP1 FISH signals, and 5 cases had 1 MRP1 signal, whereas in 4 AML patients with inv(16) no MRP1 deletions were observed. A variability in MRP activity, expressed as CF efflux-blocking by MK-571, was observed (efflux-blocking factors varied between 1.2 and 3.6); this correlated with the number of MRP1 genes (r = 0.91, P <. 01). MRP activity in the AML cases was not different from normal hematopoietic cells. MRP1 mRNA was detected in patients with 1 or 2 MRP1 FISH signals, but not in patients with no MRP1 signals. MRP2 and MRP6 mRNA were expressed predominantly in AML samples with 1 MRP1 signal, whereas in normal bone marrow cells no MRP2 and MRP6 mRNA was observed. In conclusion, this study shows that MRP activity varies among inv(16) AML cases and does not differ from that in normal hematopoietic cells; this might be in part due to the up-regulation of other MRP genes.


Asunto(s)
Inversión Cromosómica , Cromosomas Humanos Par 16 , Proteínas de Unión al ADN/genética , Resistencia a Múltiples Medicamentos/genética , Eliminación de Gen , Leucemia Mieloide/genética , Proteínas Asociadas a Resistencia a Múltiples Medicamentos , Enfermedad Aguda , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Disparidad de Par Base , Células de la Médula Ósea/citología , Células de la Médula Ósea/patología , Carcinoma de Células Pequeñas , Células Cultivadas , Mapeo Cromosómico , Proteínas de Unión al ADN/metabolismo , Doxorrubicina/toxicidad , Humanos , Hibridación Fluorescente in Situ , Leucemia Mieloide/tratamiento farmacológico , Leucemia Mieloide/patología , Neoplasias Pulmonares , Proteína 3 Homóloga de MutS , Valores de Referencia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Tumorales Cultivadas
4.
Genomics ; 60(2): 238-40, 1999 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-10486216

RESUMEN

In searching for a tumor suppressor gene in the 3p21.3 region, we isolated two genes, RBM5 and RBM6. Sequence analysis indicated that these genes share similarity. RBM5 and-to a lesser extent-RBM6 also have similarity to DXS8237E at Xp11.3-11.23, which maps less than 20 kb upstream of UBE1. A homologue of UBE1, UBE1L, is located at 3p21. 3. FISH analysis showed that the distance between UBE1L and RBM5 in 3p21.3 is about 265 kb. DXS8237E and UBE1 on the X chromosome have the same orientation, whereas on chromosome 3 the orientation of RBM5 and that of RBM6 are opposite to the orientation of UBE1L. Presumably, part of the Xp11.3-11.23 region has duplicated to chromosome 3. Part of this region on chromosome 3 may subsequently have duplicated again within the same chromosomal region. Inversion at some stage of the evolution of the human genome would explain the change in orientation of the genes on chromosome 3 compared with that of the genes on the X chromosome.


Asunto(s)
Evolución Biológica , Cromosomas Humanos Par 3/genética , Reordenamiento Génico , Cromosoma X/genética , Animales , Proteínas de Ciclo Celular , Deleción Cromosómica , Proteínas de Unión al ADN/genética , Duplicación de Gen , Genes Supresores de Tumor , Humanos , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Neoplasias/genética , Proteínas de Unión al ARN/genética , Proteínas Supresoras de Tumor
5.
Cancer Genet Cytogenet ; 112(1): 21-5, 1999 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-10432930

RESUMEN

The first published chromosomal pattern of the retroperitoneal lymph node metastasis of a malignant gonadal stroma cell tumor of the adult testis is presented. Karyotyping showed structural chromosomal abnormalities and loss of the Y-chromosome. This loss was confirmed in primary tumor and metastasis using fluorescence in situ hybridization (FISH). The characteristic chromosomal abnormality of adult testicular germ cell tumors, an i(12p), was not present. The results are compared with other data of testicular and ovarian sex cord stromal tumors. From the comparison of the male tumors, it is concluded that loss of the Y-chromosome might have a pathogenetic significance.


Asunto(s)
Deleción Cromosómica , Tumores de los Cordones Sexuales y Estroma de las Gónadas/genética , Cromosoma Y , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Metástasis Linfática , Masculino , Metafase , Persona de Mediana Edad , Tumores de los Cordones Sexuales y Estroma de las Gónadas/patología
6.
Am J Med Genet ; 86(2): 168-73, 1999 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-10449655

RESUMEN

To date, approximately 30 patients have been described with a tetrasomy 9p, all being caused by the presence of an isochromosome 9p. We now report on a 3-year-old boy with a de novo intrachromosomal triplication of 9p13-p22, resulting in partial tetrasomy 9p. We compared his phenotype with cases of tetrasomy 9p caused by the presence of an extra isochromosome 9p. He has facial anomalies similar to those of cases of tetrasomy 9p, central nervous system abnormalities, and severe psychomotor retardation but no other major congenital anomalies. Fluorescence in situ hybridization with region-specific probes showed that the middle repeat of the triplicated part is inverted. Microsatellite analysis demonstrated an involvement of both paternal chromosome 9 homologues in the triplication. This is compatible with either unequal crossing over of three of the four chromatids in paternal meiosis I or with a double crossing over in meiosis I and II (or an early mitotic division).


Asunto(s)
Aneuploidia , Aberraciones Cromosómicas/genética , Cromosomas Humanos Par 9/genética , Adulto , Sistema Nervioso Central/anomalías , Niño , Preescolar , Bandeo Cromosómico , Cara/anomalías , Femenino , Feto , Humanos , Hibridación Fluorescente in Situ , Lactante , Recién Nacido , Cariotipificación , Masculino , Fenotipo , Embarazo , Trastornos Psicomotores/patología
8.
Eur J Vasc Endovasc Surg ; 17(1): 9-14, 1999 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-10071611

RESUMEN

OBJECTIVES: This study compared the efficiency of electroporation and synthetic amphiphiles. (SAINT-2pp/DOPE) in transfecting small numbers of human endothelial cells. METHODS AND RESULTS: Optimal transfection conditions were tested and appeared to be 400 V and 960 microF for electroporation and a 10:1 ratio for concentrations of SAINT-2pp/DOPE: plasmid. Using these conditions, cell concentrations were lowered step-wise and we were able to transfect as few as one thousand cells with both methods. For detection of transfection of a small number of cells a sensitive assay was needed (Luciferase). A plasmid containing the neomycin resistance gene was used to determine the transfection rate expressed in colony forming units by counting colonies after selection. At low plasmid concentrations this transfection rate was within the same range for both electroporation and SAINT-2pp/DOPE transfection. Fluorescent in situ hybridisation of metaphase chromosomes of transfected endothelial cells using the plasmid as a probe showed that stable integration was possible with both methods. CONCLUSIONS: Electroporation and a synthetic amphiphile, SAINT-2pp, provide the possibility of transfecting small numbers of cells resulting in stable integration of low plasmid concentrations. The availability of this technology is important in order to obtain functional endothelial cell lines from various human blood vessels for research purposes.


Asunto(s)
Electroporación , Endotelio Vascular/citología , Plásmidos , Transfección , Línea Celular Transformada , Cloranfenicol O-Acetiltransferasa/genética , Regulación Enzimológica de la Expresión Génica/fisiología , Genes Reporteros , Humanos
9.
Int J Cancer ; 77(2): 217-8, 1998 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-9650555

RESUMEN

Cytogenetic investigation of a malignant ovarian tumor diagnosed as a mixed germ-cell tumor, composed of extensive choriocarcinoma and foci of yolk-sac tumor, revealed a highly abnormal chromosomal pattern. We found a chromosome number in the hypertriploid/hypotetraploid range, and several clonal structural abnormalities, including 2 copies of an isochromosome 12p. We showed that the chromosomal pattern of this ovarian tumor is very similar to that of testicular germ-cell tumors. This finding, together with reported cytogenetic data of malignant ovarian germ-cell tumors, supports the hypothesis that ovarian and testicular germ-cell tumors are strongly related entities that may have a similar origin and pathogenetic pathway.


Asunto(s)
Aberraciones Cromosómicas , Germinoma/genética , Neoplasias Ováricas/genética , Adulto , Aneuploidia , Cromosomas Humanos Par 12 , Femenino , Humanos , Isocromosomas , Cariotipificación
10.
Int J Cancer ; 73(4): 521-4, 1997 Nov 14.
Artículo en Inglés | MEDLINE | ID: mdl-9389566

RESUMEN

Renal oncocytomas reveal a considerable (cyto)genetic heterogeneity. At least 2 genetic subsets are currently recognized, characterized by (1) translocations involving breakpoint 11q13 and (2) the combined loss of chromosomes 1 and X/Y. We present a case of oncocytoma revealing a 3-way translocation involving breakpoint 11q13, a der(1)t(1;8) and an add(19). The der(1) resulted in loss of chromosome 1 sequences. Using fluorescence in situ hybridization, the 11q13 breakpoint of the present case proved to be slightly different from the one observed previously in 3 cases of renal oncocytoma. Whether the 11q13 breakpoint observed in our case resides in or near another gene remains to be elucidated.


Asunto(s)
Adenoma Oxifílico/genética , Deleción Cromosómica , Cromosomas Humanos Par 11/genética , Cromosomas Humanos Par 12/genética , Cromosomas Humanos Par 19/genética , Cromosomas Humanos Par 1/genética , Cromosomas Humanos Par 5/genética , Cromosomas Humanos Par 8/genética , Neoplasias Renales/genética , Translocación Genética/genética , Humanos , Masculino , Persona de Mediana Edad
11.
Genes Chromosomes Cancer ; 19(4): 220-7, 1997 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-9258656

RESUMEN

The recently identified FHIT gene encompasses the FRA3B region and the breakpoint of a constitutive t(3;8) occurring in a family with hereditary renal cell cancer. Occurrence of aberrant transcripts in different types of tumours has led to the suggestion that FHIT might play a critical role in the development of various types of cancer. We have analyzed the gene and its transcripts in lung cancers and renal cell cancer-derived cell lines. A lung adenocarcinoma cell line, GLC-A2, appeared to have a homozygous deletion in intron 5 of FHIT. RT-PCR analysis revealed a normal-sized PCR product in all of the cell lines: Including GLC-A2. A number of them had an additional aberrant product. Analysis of a great number of control cell lines and tissues showed that the majority of these also had aberrant PCR products in addition to a normal-sized PCR product. Different specimens of the same cell type showed variable additional RT-PCR products. Normal-sized PCR products had a sequence identical to the FHIT sequence. PCR products longer than normal had insertions of different sizes at different positions. With three exceptions, PCR products shorter than normal represented FHIT sequences missing one or more entire exons. Thus, the presence of aberrant transcripts is not cancer-specific. Conceivably, sequence responsible for the instability of the FRA3B region are being transcribed into FHIT pre-mRNA and may cause the abnormal splicing and processing of the transcripts.


Asunto(s)
Ácido Anhídrido Hidrolasas , Neoplasias Renales/genética , Neoplasias Pulmonares/genética , Proteínas de Neoplasias/genética , Proteínas/genética , Adenocarcinoma/genética , Carcinoma de Células Pequeñas/genética , Carcinoma de Células Escamosas/genética , Deleción Cromosómica , Mapeo Cromosómico , Cromosomas Artificiales de Levadura , Homocigoto , Humanos , Hibridación Fluorescente in Situ , Repeticiones de Microsatélite , Reacción en Cadena de la Polimerasa , ARN Neoplásico/aislamiento & purificación , Análisis de Secuencia de ARN , Transcripción Genética/genética , Células Tumorales Cultivadas
12.
Biochim Biophys Acta ; 1352(2): 123-8, 1997 May 30.
Artículo en Inglés | MEDLINE | ID: mdl-9199240

RESUMEN

We isolated and sequenced cDNA and genomic DNA fragments of the human CPT1B gene, encoding muscle type camitine palmitoyltransferase I. A recombinant P1 phage containing CPT1B was mapped to chromosome 22qter by fluorescent in situ hybridization. This finding supports the concept that 'liver type' and 'muscle type' isoforms of CPT I are encoded by different loci at separate chromosomal positions. Analysis of CPT1B cDNA sequences revealed the presence of an untranslated 5' exon and differential processing of introns 13 and 19. The alternative splicing of intron 13 causes an in-frame deletion leading to a 10 amino acid residues smaller protein. Using different splice acceptor sites, intron 19 is spliced in the majority of cases, but 4 out of 14 sequenced CPT1B 3' cDNA clones contain part of intron 19 in stead of exon 20. We found that differential polyadenylation is the mechanism behind the existence of these alternative 3' CPT1B mRNA forms.


Asunto(s)
Carnitina O-Palmitoiltransferasa/genética , Músculos/enzimología , Secuencia de Bases , Mapeo Cromosómico , ADN Complementario/aislamiento & purificación , Humanos , Hibridación in Situ , Intrones , Datos de Secuencia Molecular
13.
Cytogenet Cell Genet ; 72(2-3): 225-8, 1996.
Artículo en Inglés | MEDLINE | ID: mdl-8978783

RESUMEN

Starting from five markers, with a well-defined order from distal to proximal 3p21, nine other markers could be inserted in this 3p21 map. Five were precisely mapped genetically. The other markers were ordered by FISH and/or deletion hybrid mapping. The overall 3p21 order from distal to proximal is as follows: D3S1298-D3S1260-(D3S966, D3S1448 (= D3S1449)-D3S1029-D3S32-D3S643-D3F15S2 -D3S2968-D3S1235-D3S1289-D3S1447-D3S1295.


Asunto(s)
Cromosomas Humanos Par 3/genética , Marcadores Genéticos , Mapeo Cromosómico , Humanos , Polimorfismo Genético , Secuencias Repetitivas de Ácidos Nucleicos
14.
Hum Genet ; 96(1): 116-8, 1995 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-7607644

RESUMEN

Seven polymorphic cosmids previously assigned to 10cen-q11.2 were mapped between D10S34 and RBP3, and ordered by interphase in situ hybridization and yeast artificial chromosome analysis. Some of the presumed unique sequences from the centromeric region have homologies either within the same region or within the centromeric region of other chromosomes.


Asunto(s)
Centrómero/genética , Cromosomas Humanos Par 10 , Mapeo Cromosómico , Marcadores Genéticos , Humanos , Hibridación in Situ , Metafase , Polimorfismo Genético
15.
Prenat Diagn ; 15(5): 467-73, 1995 May.
Artículo en Inglés | MEDLINE | ID: mdl-7644437

RESUMEN

We report two cases of apparently balanced complex de novo chromosomal rearrangements (BCCR) detected prenatally at 17 weeks and 10 weeks of gestation, respectively. Chromosomes were studied using GTG-banding and fluorescent in situ hybridization (FISH). In one case four chromosomes and in the other case three chromosomes were involved in the rearrangements. One of the pregnancies was terminated and no external or internal showed no abnormalities. The child is now 3 years old and has neither congenital anomalies nor evidence of delayed psychomotor development.


Asunto(s)
Aberraciones Cromosómicas , Diagnóstico Prenatal , Adulto , Amniocentesis , Muestra de la Vellosidad Coriónica , Femenino , Estudios de Seguimiento , Humanos , Hibridación Fluorescente in Situ , Cariotipificación , Masculino , Edad Materna , Embarazo , Embarazo de Alto Riesgo
16.
Genes Chromosomes Cancer ; 12(3): 224-8, 1995 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-7536463

RESUMEN

In a family with a constitutional translocation t(3;6), the oldest member carrying the translocation had developed multiple nonpapillary renal cell carcinomas (RCCs). The translocation breakpoint was positioned between 3p13 and 3p14.1. This is close to the region in which a t(3;8) breakpoint has been reported in a family with hereditary RCC. We defined the location of the t(3;6) and t(3;8) breakpoints by fluorescence in situ hybridization (FISH) analysis with yeast artificial chromosomes (YACs) from the 3p14-13 region. Both interphase nuclei and metaphase cells from translocation-carrying members of both families have been used, allowing the definition of flanking YACs for each breakpoint. We could thereby clearly confirm that the breakpoints are different, the t(3;8) breakpoint being most distal. In addition, we have shown that both translocation breakpoints are located distal to the homozygously deleted region in the U2020 lung cancer cell line.


Asunto(s)
Carcinoma de Células Renales/genética , Cromosomas Humanos Par 3 , Cromosomas Humanos Par 6 , Neoplasias Renales/genética , Translocación Genética , Células Cultivadas , Mapeo Cromosómico , Cromosomas Artificiales de Levadura , Cromosomas Humanos Par 8 , Fibroblastos , Humanos , Hibridación Fluorescente in Situ
17.
Cancer Res ; 54(15): 4183-7, 1994 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-8033151

RESUMEN

All types of lung carcinoma are characterized by a high frequency of loss of sequences from the short arm of chromosome 3, the smallest region of overlap containing D3F15S2 in band p21. Here we characterize a 440-kilobase segment from this region, which we found homozygously deleted in one of our small cell lung cancer-derived cell lines. The homozygous deletion maps between UBE1L and ZnF16, just centromeric to D3F15S2. Yeast artificial chromosomes with inserts originating from the deleted region are very unstable and readily lose parts of their insert.


Asunto(s)
Carcinoma de Células Pequeñas/genética , Deleción Cromosómica , Cromosomas Artificiales de Levadura , Cromosomas Humanos Par 3 , Neoplasias Pulmonares/genética , Secuencia de Bases , Homocigoto , Humanos , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Células Tumorales Cultivadas
18.
Am J Med Genet ; 47(4): 559-62, 1993 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-7504882

RESUMEN

We describe a 1-year-old boy with a rare de novo 46,XY/47,XY, + i(5p) mosaicism (ratios 28/3 in peripheral blood lymphocytes and 2/12 in skin fibroblasts). The boy, born after a pregnancy of 34 weeks, had lung hypoplasia, persistent hypotonia, and postnatal growth failure. Craniofacial anomalies were also present. His clinical manifestations correspond to those described in trisomy 5p patients. Prenatal diagnosis on maternal age indication had shown normal male chromosomes in 16 cells in the short term culture of a chorionic villus sampling. Retrospectively, 1 out of 217 cells in this culture showed the i(5p). Several mechanisms could have resulted in the formation of this 46/47, + i(5p) mosaic. Postzygotic local incorrect ligation during chromatid replication, followed by a second replication offers an attractive model on theoretical grounds since it needs only one step to explain both isochromosome formation and mosaicism. Differences between the various tissues in selection pressure on cells with the isochromosome might explain the different ratios of mosaicism found.


Asunto(s)
Aneuploidia , Aberraciones Cromosómicas/genética , Cromosomas Humanos Par 5 , Discapacidades del Desarrollo/genética , Mosaicismo , Bandeo Cromosómico , Trastornos de los Cromosomas , Cara/anomalías , Humanos , Recién Nacido , Cariotipificación , Pulmón/patología , Masculino , Cráneo/anomalías
19.
Hum Genet ; 91(5): 504-6, 1993 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-8314565

RESUMEN

D13S31 is the marker closest to the Wilson disease locus according to genetic analysis. Its physical localisation was refined by fluorescent in situ hybridisation to the junction to chromosomal bands 13q14.3 and 13q21.1. Using polymerase chain reaction analysis, D13S31 and D13S59 (the closest proximal and distal marker, respectively) were found to be located on the end of the der(13) consisting of 13pter-13q14.3: in the somatic cell hybrid ICD, and to be absent from the cell lines WC-H38B3B6 containing a del(13) (13pter-q13::13q21.1-qter) and KSF39 containing a del(13) (13pter-q14.1:).


Asunto(s)
Cromosomas Humanos Par 13 , Eliminación de Gen , Degeneración Hepatolenticular/genética , Animales , Secuencia de Bases , Mapeo Cromosómico/métodos , Cricetinae , Análisis Mutacional de ADN , Humanos , Células Híbridas , Hibridación Fluorescente in Situ , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa
20.
Genes Chromosomes Cancer ; 6(1): 10-6, 1993 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-7680216

RESUMEN

A method combining flow sorting and molecular cytogenetic techniques for the identification of unknown marker chromosomes is described. In this study, the bladder tumor cell line J82 was used, which was known to carry a marker chromosome of the size of chromosome 7 in every cell. From the cytogenetic analysis of Q-banded metaphase cells, it was shown to be composed of approximately 40% presumably the greater part of chromosome 20 and for the rest microscopically unidentifiable material. This marker chromosome was found using flow cytometric analysis to form an independent peak and hence was suitable for isolation using dual-parameter sorting after staining with Hoechst 33258 and chromomycin A3. Subsequently, the marker was isolated by dual-parameter sorting. DNA amplification of 300 isolated chromosomes by polymerase chain reaction (PCR) using the Alu-primer Bk33 and the LINES-primer LH5 was carried out. After purification of the amplified product, a yield of 5 microns of DNA was obtained. The DNA was labelled using Bio-11-dUTP and applied to human lymphocyte metaphase cells in a suppressive in situ hybridization procedure. Fluorescence was visible over chromosome 20 and over the distal one-half of 6p. Together the fluorescent regions accounted for only approximately 60% of the marker length, indicating a possible duplication of chromosome 20 material. This was confirmed by applying bicolor in situ hybridization using chromosome 6- and 20-specific DNA libraries to metaphase cells of the J82 cells.


Asunto(s)
Carcinoma de Células Transicionales/genética , Cromosomas Humanos Par 13 , Cromosomas Humanos Par 20 , Translocación Genética , Neoplasias de la Vejiga Urinaria/genética , Aneuploidia , ADN de Neoplasias/análisis , Citometría de Flujo , Marcadores Genéticos , Humanos , Hibridación Fluorescente in Situ , Reacción en Cadena de la Polimerasa , Células Tumorales Cultivadas
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