[Synthesis of some thiourea derivatives and their influence on biological and genetic effects of gamma rays]. / Sintez nekotorykh proizvodnykh tiomocheviny i ikh vliianie na biologicheskoe i geneticheskoe vozdeistvie gamma-luchei.

The synthesis of the new radioprotective compounds ATB (I, 2-allylthioureidobenzoic acid), PTB (II, 2-phenylthioureidobenzoic acid), A-2-PTU (III, N-allyl-N'-2-pyridylthiourea), and P-2-PTU (IV, N-phenyl-N'-2-pyridylthiourea) and their influence on biological and genetic effects of gamma rays was studied. In result of investigations it must be noted that PTB displayed radioprotective effect as a result of which more plants in M1 germination and survive in M2 of the induced mutations is increased. The cytological analysis reveals that the studied substance (PTB) decreases chromosome aberration in meristem cells of pea roots almost twice as a result of postirradiation treatment. The effect of A-2-PTU in the experiments with peas greatly depends on the dose of irradiation, i.e., on the degree of damaging of the processes of cell restoration and the possibility of their partial restoration after the treatment with the protector. The results obtained suggest that chemical compounds of N,N'-disubstituted thiourea group (A-2-PTU and P-2-PTU) exert strong radioprotective effect in the experiments with peas. This is of great importance to modern radiobiology and radiation mutagenesis and also to protect hereditary structures against radiation.

Enhanced inhibition of plant virus replication by pyridylthiourea compounds complexed with metal ions.

Inhibition of potato virus X replication can be enhanced by complexation of N-allyl-N'-2-pyridyl-thiourea and of N-phenyl-N'-2-pyridyl-thiourea with Cu+, Zn2+ and Cd2+. The antiphytoviral activity of some complexes with pyridylthiourea can be further augmented by adding small quantities of 2,4-dioxohexahydro-1,3,5-triazine.

Antirival activity of N-phenyl-N'-arylthiourea derivatives against some Rhinoviruses.

The effect of 12 derivatives of N-phenyl-N'-aryl- or alkylthiourea, inhibitors of human enteroviruses and foot-and-mouth disease virus, on reproduction of some rhinoviruses (H-17, B-632) in HeLa Bristol cells was studied. As screening methods both the multicylce growth test in roller tube cultures and two variants of plaque inhibition tests were employed. The compounds selected were tested in one-step growth cycle set-up. We established that N-phenyl-N'-4-hydroxyphenylthiourea (V-24) and N-phenyl-N'-2-carboxyphenylthiourea (V-17) have a distinct inhibitory effect on the growth of rhinovirus H-17, and N-phenyl-N'-2-hydroxy-5-nitrophenylthiourea (V-25) inhibited strongly the multiplication of rhinovirus B-632.