American cutaneous leishmaniasis in horses from endemic areas in the north-central mesoregion of Paraná state, Brazil.

American cutaneous leishmaniasis (ACL) is an endemic disease in the north-west of Paraná state, Brazil, where it occurs in humans and dogs. Few studies have been undertaken on the occurrence of the disease in other domestic animals and horses. In this study we investigated the infection of horses by Leishmania in ACL-endemic rural areas. Direct agglutination test (DAT) and polymerase chain reaction (PCR) were employed. Fifty-five horses from rural areas in the municipalities of Doutor Camargo, Ourizona, São Jorge do Ivaí, Ivatuba and Santa Fé (Agua do O) were analysed. DAT-detected antibody titres varied from 10 to 640, and 42 (76.3%) animals presented titres > or =20. PCR analyses led to the detection of Leishmania (Viannia) DNA in the blood samples of three (7.1%) of 42 animals. The presence of anti-L. (V.) braziliensis antibodies and Leishmania (Viannia) DNA in horses from endemic areas suggests their participation in the ACL transmission cycle.

[Serologic survey of dogs in an endemic area of tegumentary leishmaniasis in Paraná State, southern Brazil] / Investigação sorológica em cães de área endêmica de leishmaniose tegumentar, no Estado do Paraná, Sul do Brasil.

Increased reporting of mucocutaneous leishmaniasis in the State of Paraná points to the need for information on both this disease's epidemiology and pertinent control measures. A serological survey was thus performed, with indirect immunofluorescence (IIF) for canine leishmaniasis in farm operations belonging to the Companhia Melhoramentos Norte do Paraná, in Jussara and Terra Boa counties, in northwestern Paraná. IIF was performed on 132 dogs, of which 24 (18.2%) had significant titers (>/=40). Imprints of six dogs with lesions were made and all were negative for Leishmania sp.