1.
Biochem Biophys Res Commun
; 224(1): 37-42, 1996 Jul 05.
Artículo
en Inglés
| MEDLINE
| ID: mdl-8694830
RESUMEN
Partly purified 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoyl-CoA oxidase from rabbit liver peroxisomes was found to convert the 25S- but not the 25R diastereoisomer of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-27-oyl-CoA into (24E)-3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholest-24-en-27-oic acid. In the presence of a peroxisomal THCA-CoA racemase, however, also the 25R isomer was oxidized. Since the mitochondrial steroid-27-hydroxylase, responsible for formation of THCA, is 25R specific a racemase seems to be obligatory for formation of cholic acid by the normal peroxisomal-dependent pathway.
Asunto(s)
Acilcoenzima A/metabolismo , Colestanoles/metabolismo , Hígado/enzimología , Microcuerpos/enzimología , Oxidorreductasas/metabolismo , Animales , Cromatografía en Gel , Cromatografía Líquida de Alta Presión , Cinética , Masculino , Mitocondrias Hepáticas/enzimología , Conejos , Estereoisomerismo , Especificidad por Sustrato
2.
Arch Dis Child
; 48(11): 850-5, 1973 Nov.
Artículo
en Inglés
| MEDLINE
| ID: mdl-4761053