Comparative characterisation of an ecto-5'-nucleotidase (CD73) in non-tumoral MCF10-A breast cells and triple-negative MDA-MB-231 breast cancer cells.

Ecto-5'-nucleotidase (CD73) hydrolyses 5'AMP to adenosine and inorganic phosphate. Breast cancer cells (MDA-MB-231) express high CD73 levels, and this enzyme has been found to play a tumour-promoting role in breast cancer. However, no studies have sought to investigate whether CD73 has differential affinity or substrate preferences between noncancerous and cancerous breast cells. In the present study, we aimed to biochemically characterise ecto-5'-nucleotidase in breast cancer cell lines and assess whether its catalytic function and tumour progression are correlated in breast cancer cells. The results showed that compared to nontumoral breast MCF-10A cells, triple-negative breast cancer MDA-MB-231 cells had a higher ecto-5'-nucleotidase expression level and enzymatic activity. Although ecto-5'-nucleotidase activity in the MDA-MB-231 cell line showed no selectivity among monophosphorylated substrates, 5'AMP was preferred by the MCF-10A cell line. Compared to the MCF-10A cell line, the MDA-MB-231 cell line has better hydrolytic ability, lower substrate affinity, and high inhibitory potential after treatment with a specific CD73 inhibitor α,ß­methylene ADP (APCP). Therefore, we demonstrated that a specific inhibitor of the ecto-5-nucleotidase significantly reduced the migratory and invasive capacity of MDA-MB-231 cells, suggesting that ecto-5-nucleotidase activity might play an important role in metastatic progression.

Polymyxin Resistance in Salmonella: Exploring Mutations and Genetic Determinants of Non-Human Isolates.

Until 2015, polymyxin resistance was primarily attributed to chromosomal mutations. However, with the first report of mobile colistin resistance (mcr-1) in commensal Escherichia coli from food animals in China, the landscape has changed. To evaluate the presence of polymyxin resistance in Salmonella spp., a drop screening test for colistin and polymyxin B was carried out on 1156 isolates of non-human origin (animals, food, and the environment), received in Brazil, between 2016 and 2021. Subsequently, 210 isolates with resistant results in the drop test were subjected to the gold-standard test (broth microdilution) for both colistin and polymyxin B. Whole-genome sequencing (WGS) of 102 resistant isolates was performed for a comprehensive analysis of associated genes. Surprisingly, none of the isolates resistant to colistin in the drop test harbored any of the mcr variants (mcr-1 to mcr-10). WGS identified that the most common mutations were found in pmrA (n= 22; T89S) and pmrB (n = 24; M15T, G73S, V74I, I83A, A111V). Other resistance determinants were also detected, such as the aac(6')-Iaa gene in 72 isolates, while others carried beta-lactamase genes (blaTEM-1blaCTX-M-2, blaCMY-2). Additionally, genes associated with fluoroquinolone resistance (qnrB19, qnrS1, oqxA/B) were detected in 11 isolates. Colistin and polymyxin B resistance were identified among Salmonella from non-human sources, but not associated with the mcr genes. Furthermore, the already-described mutations associated with polymyxin resistance were detected in only a small number of isolates, underscoring the need to explore and characterize unknown genes that contribute to resistance.

Detection and genomic characterization of a multidrug-resistant Salmonella Newport co-harbouring blaCMY-2, qnrB19 and mcr-9 from the diarrheic faeces of a foal.

OBJECTIVES: This study reports the genomic characterization of the multidrug resistant Salmonella Newport strain 195_20 recovered from the diarrheic faeces of a foal in Brazil and co-harbouring the mcr-9, blaCMY-2 and qnrB19 antibiotic resistance genes. METHODS: Bacterial isolate positive for mobile colistin resistance gene (mcr-9) was submitted to antimicrobial susceptibility testing by disk diffusion and broth microdilution for colistin and polymyxin B. The isolate was submitted to whole genome sequencing by Illumina technology and Nanopore Sequencing. Conjugation assays, plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinIon long reads sequencing. RESULTS: Isolate 195_20 was identified as sequence type ST45, resistant to penicillin and cephalosporins (ampicillin, ceftazidime, ceftriaxone and cefotaxime), aminoglycosides (streptomycin and gentamicin), phenicol (chloramphenicol), quinolones and fluoroquinolones (nalidixic acid, ciprofloxacin, and pefloxacin), folate pathway antagonists (sulfonamides and trimethoprim-sulfamethoxazole), and tetracycline. A transferable IncHI2/IncHI2A plasmid sized ca. 262kb was found to carry the mcr-9 gene in a module consisting of IS903-mcr-9-wbuC-IS26. In addition, an 174kb IncC and a 48kb IncN plasmid were also identified in the 195_20 isolate, carrying blaCMY-2 and qnrB19, respectively. CONCLUSIONS: Not surprisingly, isolate 195_20 was susceptible to polymyxins, possibly due to absence of qseBC regulatory operon. Presence of mobile colistin resistance (mcr-9), third-generation cephalosporins (blaCMY-2) and quinolone (qnrB19) resistance determinants in zoonotic pathogens from animals in close contact with humans alerts for the possible route of transmission between these different reservoirs.

Influence of different ultra-soft toothbrushes on erosive tooth wear.

OBJECTIVE: To investigate the influence of different ultra-soft toothbrushes on the progression of erosive tooth wear (ETW). METHODS: Bovine enamel and dentin specimens (n = 10) were submitted to a 5-day erosive-abrasive cycling model (0.3% citric acid for 5 min, artificial saliva for 60 min, 4x/day). Toothbrushing was carried out 2x/day for 15 s, with the different toothbrushes tested (A- Edel White: flexible handle, tapered bristles; B- Oral-B Gengiva Detox: regular handle, criss-cross tapered bristles; C- Colgate Gengiva Therapy: flexible handle, tapered bristles, high tuft density; d- Oral-B Expert Gengiva Sensi: regular handle, round end bristles, high tuft density; E- Oral-B Indicator Plus: soft brush, round end bristles (control). Surface loss (SL, in µm) was assessed by optical profilometry. The toothbrush characteristics were evaluated by a surgical microscope. Data were statistically analyzed (α=0.05). RESULTS: For enamel, toothbrush C showed the highest SL (means±SD: 9.86 ± 1.28) and it did not differ significantly from A (8.60 ± 0.50), both with flexible handles. The lowest SL was observed for the toothbrush Control E (6.76 ± 0.63), which differed significantly from A and C, but not from the other toothbrushes. For dentin, the highest SL was found for toothbrush D (6.97 ± 1.05) and it did not differ significantly from E (6.23 ± 0.71). The lowest SL was observed for B (4.61 ± 0.71) and C (4.85 + 0.83), without significant differences from A (5.01 ± 1.24). CONCLUSIONS: The ultra-soft toothbrushes had different impacts on the progression of ETW on the dental substrates. On enamel, higher ETW values were observed for the flexible handle toothbrushes, while for dentin, round-end bristles (ultra-soft and soft) caused more ETW. CLINICAL SIGNIFICANCE: Knowledge about the effect of different ultra-soft toothbrushes on ETW can help clinicians to recommend the most suitable types for their patients, bearing in mind that toothbrushes can impact enamel and dentin differently.

Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil.

This study analyzes the genomic findings of the first report of Salmonella isolate carrying the blaCTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the blaCTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric test. Whole genome sequencing was performed using Illumina technology. Conjugation assay was performed; plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinION long reads sequencing. Isolate 288_18 was identified as sequence type ST13, resistant to ampicillin, cefotaxime, ceftazidime, cefepime, ceftriaxone, and aztreonam. A transferable IncFII plasmid sized approximately 67 kb was found to carry the blaTEM-1 and blaCTX-M-55 in a module consisting of IS26-blaTEM-1B-WbuC-blaCTX-M-55-IS26. In addition, an 117 kb IncI1plasmid was also identified in the 288_18 isolate, but without additional resistance genes. To the best of our knowledge, this is the first report of blaCTX-M-55 in Salmonella isolated from human infection in Brazil. The occurrence of blaCTX-M-55 in the IncFII epidemic plasmid in a relevant clinical human isolate of Salmonella Agona underscores the urgent need for enhanced and effective continuous surveillance for controlling its dissemination.

Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil

This study analyzes the genomic findings of the first report of Salmonella isolate carrying the blaCTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the blaCTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric test. Whole genome sequencing was performed using Illumina technology. Conjugation assay was performed; plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinION long reads sequencing. Isolate 288_18 was identified as sequence type ST13, resistant to ampicillin, cefotaxime, ceftazidime, cefepime, ceftriaxone, and aztreonam. A transferable IncFII plasmid sized approximately 67 kb was found to carry the blaTEM-1 and blaCTX-M-55 in a module consisting of IS26-blaTEM-1B-WbuC-blaCTX-M-55-IS26. In addition, an 117 kb IncI1plasmid was also identified in the 288_18 isolate, but without additional resistance genes. To the best of our knowledge, this is the first report of blaCTX-M-55 in Salmonella isolated from human infection in Brazil. The occurrence of blaCTX-M-55 in the IncFII epidemic plasmid in a relevant clinical human isolate of Salmonella Agona underscores the urgent need for enhanced and effective continuous surveillance for controlling its dissemination.

New insights into perceptions of technology claims in greek-style yogurt: A view in the COVID-19 pandemic.

The Covid-19 pandemic has strongly impacted people's lives and the food industry. In this sense, food products claiming nutritional and health-promoting benefits due to the presence of bioactive peptides and probiotics, such as Greek-style yogurt, have been in demand. The objective of this work was to investigate, through word association, the perception of the consumers regarding the seven concepts related to Greek-style yogurt (traditional, ultra-creamy, zero fat, high content proteins, zero lactose, light and with no added sugars), in the context of social isolation due to Covid-19. In this online survey, 346 participants completed a questionnaire. The participants were divided according to health concerns (increased, not changed, or decreased) and eating habits (improved, not changed, or worsened) during the Covid-19 pandemic. Chi-square and prototypical analysis were used as statistical tests. During the Covid-19 pandemic, based on self-report, around 66% of the participants had their eating habits and their concerns about health changed. The general associations were related to the categories pleasure, health, creamy, pleasant texture, food restriction, and loss of sensory quality. 'Health' and 'pleasure' were negatively associated with the conceptualization of Greek-style yogurt. For the zero-fat, light, and sugar-free Greek-style yogurts, the terms creamy and ultra-creamy are sensory appealing to the consumers. In general, the price and concerns about health are factors that strongly influence the purchase intention of Greek-style yogurts. The yogurts were associated with sensory and non-sensory characteristics, which can be useful for marketing strategies for of different product concepts.

Social support and depressive symptoms in older adulsts treated in an outpatient service / Apoyo social y síntomas depresivos en ancianos atendidos en un servicio ambulatorio / Apoio social e sintomas depressivos em idosos atendidos em um ambulatório

ABSTRACT Objective: to assess social support, to screen the scores indicative of depression, and to identify if social support is associated in any way with the scores and with the sociodemographic variables. Method: a cross-sectional and analytical study conducted at a Specialty Outpatient Service for Older Adults in São Paulo. A total of 133 aged individuals were selected from February 2019 to July 2021. During data collection, a structured questionnaire with diverse sociodemographic and clinical information was used, as well as having a caregiver or not; in addition, the following instruments were applied: Mini-Mental State Examination, Geriatric Depression Scale, Katz, Lawton and Social Support Scale. Results: the study participants' mean age was 74.2 years old, they were mostly women (72.9%), married (35.3%), white-skinned (70.7%) and retired (74.5%), they had four years of study (31.6%) and incomes up to one minimum wage (30.8%), and they were hypertensive (73.6%), diabetic (38.3%) and dyslipidemic (31.5%). It was observed that, in all the Social Support Scale domains, most of the interviewees perceived high support. It was evidenced that the highest frequency of aged people with normal psychological state corresponded to those with the highest frequency of high support perception in the Emotional and Positive social interaction domains. Conclusion: the study was able to evidence that aged people with a low perception of social support were the ones that presented more depressive symptoms. Therefore, by evidencing and knowing the sociodemographic profile of the service, it is possible to favor planning of the care provided by the multiprofessional team and propose strategic actions for comprehensive care.

RESUMEN Objetivo: evaluar el apoyo social, rastrear las puntuaciones que indican depresión e identificar si hay alguna asociación entre el apoyo social con las puntuaciones y las variables sociodemográficas. Método: estudio transversal y analítico realizado en el Servicio Ambulatorio de Especialidades para Ancianos de San Pablo. Se seleccionaron 133 adultos mayores entre febrero de 2019 y julio de 2021. En la recolección de datos se utilizó un cuestionario estructurado con diversa información sociodemográfica y clínica o tener cuidador o no, además de aplicarse los siguientes: Mini-Examen de Estado Mental, Escala de Depresión Geriátrica, Katz, Lawton y Escala de Apoyo Social. Resultados: la media de edad de los participantes del estudio fue de 74,2 años, la mayoría eran mujeres (72,9%), personas casadas (35,3%), de raza blanca (70,7%), jubilados (74,5%), con cuatro años de estudio (31,6%), ingresos de hasta un salario mínimo (30,8%), y con hipertensión (73,6%), diabetes (38,3%) y dislipidemia (31,5%). Se observó que en todos los dominios de la Escala de Apoyo Social, la mayoría de los entrevistados indicó una percepción de apoyo elevado. Se hizo evidente que la mayor frecuencia de personas mayores con estado psicológico normal correspondió con aquellas con mayor frecuencia de percepción de apoyo elevado en los dominios Emocional e Interacción social positiva. Conclusión: el estudio logró evidenciar que los ancianos que presentaron una percepción de apoyo social bajo fueron las que tuvieron más síntomas depresivos. En consecuencia, al poner de manifiesto y conocer el perfil sociodemográfico del servicio, fue posible favorecer la planificación de la asistencia prestada por el equipo multiprofesional y proponer acciones estratégicas para la integralidad de la atención.

RESUMO Objetivo: avaliar o apoio social, rastrear os escores indicativos de depressão e identificar se há associação do apoio social com os escores e as variáveis sociodemográficas. Método: estudo transversal e analítico, realizado no Ambulatório de Especialidades do Idoso em São Paulo. Foram selecionados 133 idosos no período de fevereiro de 2019 a julho de 2021. Na coleta de dados, utilizou-se um questionário estruturado, com informações sociodemográficas, clínicas, ter ou não cuidador e foram aplicados os instrumentos: Miniexame do estado mental, Escala de Depressão Geriátrica, Katz, Lawton, Escala de apoio Social. Resultados: a idade média dos participantes do estudo foi de 74,2 anos, mulher (72,9%), casada (35,3%), branca (70,7%), aposentadas (74,5%), quatro anos de estudos (31,6%), renda de até um salário-mínimo (30,8%) hipertensas (73,6%), diabéticas (38,3%) e dislipidêmicas (31,5%). Observou-se que em todos os domínios da escala de apoio social a maior parte dos entrevistados teve percepção de apoio alto. Evidenciou-se que a maior frequência de pessoas idosas com quadro psicológico normal eram aquelas com maior frequência de percepção de apoio alto nos domínios emocional e interação social positiva. Conclusão: o estudo pode evidenciar que pessoas idosas que apresentaram percepção de apoio social baixo foram as que apresentaram mais sintomas depressivos. Portanto, ao evidenciar e conhecer o perfil sociodemográfico do serviço pode-se favorecer o planejamento do cuidado prestado pela equipe multiprofissional e propor ações estratégicas para integralidade do cuidado.

Hydrogen Peroxide Generation as an Underlying Response to High Extracellular Inorganic Phosphate (Pi) in Breast Cancer Cells.

According to the growth rate hypothesis (GRH), tumour cells have high inorganic phosphate (Pi) demands due to accelerated proliferation. Compared to healthy individuals, cancer patients present with a nearly 2.5-fold higher Pi serum concentration. In this work, we show that an increasing concentration of Pi had the opposite effect on Pi-transporters only in MDA-MB-231 when compared to other breast cell lines: MCF-7 or MCF10-A (non-tumoural breast cell line). Here, we show for the first time that high extracellular Pi concentration mediates ROS production in TNBC (MDA-MB-231). After a short-time exposure (1 h), Pi hyperpolarizes the mitochondrial membrane, increases mitochondrial ROS generation, impairs oxygen (O2) consumption and increases PKC activity. However, after 24 h Pi-exposure, the source of H2O2 seems to shift from mitochondria to an NADPH oxidase enzyme (NOX), through activation of PKC by H2O2. Exogenous-added H2O2 modulated Pi-transporters the same way as extracellular high Pi, which could be reversed by the addition of the antioxidant N-acetylcysteine (NAC). NAC was also able to abolish Pi-induced Epithelial-mesenchymal transition (EMT), migration and adhesion of MDA-MB-231. We believe that Pi transporters support part of the energy required for the metastatic processes stimulated by Pi and trigger Pi-induced H2O2 production as a signalling response to promote cell migration and adhesion.

Fatores associados à qualidade de vida de idosos hospitalizados / Factores asociados a la calidad de vida de los ancianos hospitalizados / Factors associated with the quality of life of hospitalized elderly

Objetivo: avaliar a qualidade de vida de idosos hospitalizados e sua associação as variáveis sociodemográficas, econômica, tempo de internação e ter cuidador. Método: estudo transversal, no qual foram incluídos cem participantes. A coleta de dados deu-se entre setembro de 2018 e abril de 2019. Aplicou-se o 36-Item Short Form Health Survey. Utilizaram-se os testes estatísticos coeficiente de correlação de Spearman e de Mann-Whitney. Resultados: saúde mental e aspectos físicos foram, respectivamente, os domínios da qualidade de vida com maior e menor escore. Sexo masculino, empregado, sem cuidador, maior idade e renda familiar se associaram de forma positiva com qualidade de vida; e de forma negativa maior tempo de internação e ter cuidador. Conclusão: as variáveis associadas à qualidade de vida foram sexo, idade, ocupação, maior renda familiar, tempo de internação e cuidador. Os cuidados com idosos internados devem ser planejados, levando-se em consideração fatores que interferem na qualidade de vida.

Objective: to evaluate the quality of life of hospitalized elderly people and their association with sociodemographic, economic, length of stay and having a caregiver variables. Method: cross-sectional study, in which one hundred participants were included. Data collection took place between September 2018 and April 2019. The 36-Item Short Form Health Survey was applied. Spearman and Mann-Whitney correlation coefficient statistical tests were used. Results: mental health and physical aspects were, respectively, the domains of quality of life with the highest and lowest scores. Male, employed, without a caregiver, older age and family income were positively associated with quality of life; and negatively longer hospitalization and having a caregiver. Conclusion: the variables associated with quality of life were sex, age, occupation, higher family income, length of stay and caregiver. Care for hospitalized elderly should be planned, taking into account factors that interfere with quality of life.

Objetivo: evaluar la calidad de vida de los ancianos hospitalizados y su asociación con variables sociodemográficas, económicas, de estancia y de tener un cuidador. Método: estudio transversal, en el que se incluyeron cien participantes. La recolección de datos se llevó a cabo entre septiembre de 2018 y abril de 2019. Se aplicó la encuesta del 36-Item Short Form Health Survey. Se utilizaron pruebas estadísticas de coeficiente de correlación de Spearman y Mann-Whitney. Resultados: la salud mental y los aspectos físicos fueron, respectivamente, los dominios de calidad de vida con mayor y menor puntaje. Hombre, empleado, sin cuidador, mayor edad e ingresos familiares se asociaron positivamente con la calidad de vida; y hospitalización negativamente más prolongada y tener un cuidador. Conclusión: las variables asociadas a la calidad de vida fueron sexo, edad, ocupación, mayor ingreso familiar, tiempo de estadía y cuidador. La atención a los ancianos hospitalizados debe planificarse teniendo en cuenta los factores que interfieren con la calidad de vida.

Gluteus Maximus Activation during Common Strength and Hypertrophy Exercises: A Systematic Review.

The gluteus maximus (GMax) is one of the primary hip extensors. Several exercises have been performed by strength and conditioning practitioners aiming to increase GMax strength and size. This systematic review aimed to describe the GMax activation levels during strength exercises that incorporate hip extension and use of external load. A search of the current literature was performed using PubMed/Medline, SportDiscuss, Scopus, Google Scholar, and Science Direct electronic databases. Sixteen articles met the inclusion criteria and reported muscle activation levels as a percentage of a maximal voluntary isometric contraction (MVIC). The exercises classified as very high level of GMax activation (>60% MVIC) were step-up, lateral step-up, diagonal step-up, cross over step-up, hex bar deadlift, rotational barbell hip thrust, traditional barbell hip thrust, American barbell hip thrust, belt squat, split squat, in-line lunge, traditional lunge, pull barbell hip thrust, modified single-leg squat, conventional deadlift, and band hip thrust. We concluded that several exercises could induce very high levels of GMax activation. The step-up exercise and its variations present the highest levels of GMax activation followed by several loaded exercises and its variations, such as deadlifts, hip thrusts, lunges, and squats. The results of this systematic review may assist practitioners in selecting exercised for strengthening GMax.

Evaluation of polymorphisms in repair and detoxification genes in alcohol drinkers and non-drinkers using capillary electrophoresis.

Alcohol use disorder (AUD) causes about 3.3 million deaths around the world each year. It is the primary risk factor for the global burden of diseases in American countries. Long-term abuse of alcohol induces numerous molecular and biochemical changes in tissues exposed to alcohol. The toxic effects of alcohol are mediated by DNA damage through various mechanisms, such as induction of oxidative damage, DNA adducts, crosslinks, and DNA strand breaks. The main aim of the current study was to compare the frequency of SNP polymorphisms in XRCC1 (rs7997782) and GSTP1 (rs1695) genes involved in DNA repair of single strand breaks (SSB) and xenobiotic detoxification between alcohol addicts and a control group comprised of non-drinkers. Genetic polymorphisms were identified following allelic specific PCR designed to generate the amplicons containing the variants. Then amplicons were sequenced, and sequences were aligned against the human genome reference deposited in GenBank using the CLC Sequence Viewer software (version 7.6.1). The GG homozygotes in rs1695 (GSTP1) were significantly (p = 0.023) 3.8x more frequent among those with AUD when compared to the control group. No SNP variation was observed in rs7997782 (XRCC1). rs1695 variant has been associated with susceptibility to various diseases, including those related to alcohol consumption.

Avaliação da retenção e reprodutibilidade dimensional de attachments em alinhadores ortodônticos de diferentes espessuras / Evaluation of retention and dimensional reproducibility of attachments in orthodontics aligners of different thicknesses

Este estudo teve como objetivo avaliar a capacidade de retenção que os attachments proporcionam aos alinhadores ortodônticos, bem como analisar a reprodutibilidade dos attachments a partir do planejamento digital até a sua reprodução clínica. A amostra foi composta por dezesseis modelos, divididos em 2 grupos, sendo oito no grupo controle impresso (GCI), onde os attachments eram impressos no modelo e outros oito modelos no grupo experimental de resina (GER), cujos attachments foram colados manualmente a partir de uma guia de transferência. Cada grupo foi dividido em outros 4 subgrupos, de acordo com o posicionamento dos attachments em determinados dentes e orientação do tal acessório no formato retangular: canino a 2º pré-molar horizontal (HPM), canino a 1º molar horizontal (HM), canino a 2º pré-molar vertical (VPM) e canino a 1º molar vertical (VM). Placas de PETG foram termoformados para um total de 80 alinhadores, sendo 40 para o grupo GCI e outros 40 para o grupo GER nas espessuras de 0.6 mm e 0.8 mm. Os modelos dos grupos GCI e GER foram escaneados e analisados por comparação digital para avaliação da morfologia dos attachments. Em seguida, os alinhadores foram submetidos a ensaio mecânico de tração. A análise qualitativa mostrou que as bases dos attachments colados possuem excesso de resina, dificultando a criação de ângulos vivos. Ao analisar qualitativamente a morfologia do grupo GER foi possível verificar que os attachments colados tiveram suas bases em dimensões maiores que o planejado. O grupo GER apresentou melhores retenções que os grupo GCI. Não há diferença estatística na orientação do acessório e no número de dentes que possuem os dispositivos. Entretanto, há diferença estatística quanto à espessura de PETG onde as placas de 0.8 mm forneceram maior retenção. Neste caso, os attachments horizontais com colagem até os molares apresentam a maior média de retenção estatisticamente significante (p < 0.01) quando comparados aos outros attachments no grupo experimental. Conclui-se que as placas de 0.8 mm conferem retenção superior do que as de espessura 0.6 mm, e também que a combinação de attachments na horizontal com colagem de canino a molar proporcionam maior retenção quando comparados à outra orientação e ao número de dentes envolvidos. (AU)

This study aimed to evaluate the retention capacity that attachments provide to orthodontic aligners, as well as to analyze the reproducibility of attachments made from guides made for this purpose. The sample consisted on sixteen models which were divided into 2 groups, eight in the printed control group (PCG), whose attachments were printed onto the model and eight in the experimental resin group (ERG), whose attachments were bonded manually from transfer guide. Each group was subdivided into 4, according to the positioning and orientation of the attachments: horizontal premolar (HPM), horizontal molar (HM), vertical premolar (VPM) and vertical molar (VM). PETG acetate aligners were thermoformed for a total of 80 aligners, 40 for the PCG group and another 40 for the ERG group in thicknesses of 0.6 mm and 0.8 mm. The models of the PCG and ERG groups were scanned and analyzed by digital comparison to assess the morphology of the attachments. Then, the aligners were subjected to mechanical tensile testing. The qualitative analysis showed that the bases of the bonded attachments have excess resin, making it difficult to create sharp angles. When analyzing the morphology of the ERG group, it was possible to verify they had the bases in larger dimensions than planned. The ERG group had better retentions than the PCG group. There is no statistical difference in the orientation of the accessory and in the number of teeth that the devices have when the attachments are grouped. However, a statistical difference occurs regarding the observed thickness of acetate whose 0.8 mm aligners provided greater retention. In this case, the horizontal attachments with bonding up to the molars have the highest statistically significant retention average (p <0.01) when compared to the other attachments in the experimental group. It is concluded that the 0.8 mm aligners provide superior retention than those of 0.6 mm thickness, and also that the combination of horizontal attachments with molar canine bonding provides greater retention when compared to the other orientation and the number of teeth involved. (AU)

Reliability measure of the rib cage deformity by a postural assessment software in patients with adolescent idiopathic scoliosis / Medida da Confiabilidade da Deformidade da Caixa Torácica por um Software de Avaliação Postural em Pacientes com Escoliose Idiopática do Adolescente

Abstract Adolescent idiopathic scoliosis (AIS) is a three-dimensional deformity of the spine that generates changes in the biomechanics of the rib cage. Digital photogrammetry enables the recording of subtle changes and the interrelationship between parts of the human body that are difficult to measure by other means. The aim of this study was to create angles and thoracic distances and to evaluate the interobserver and intraobserver reliability of these measurements using the Software de Avaliação Postural (SAPO) in patients with AIS. This cross-sectional study evaluated 30 individuals aged between 11 and 18 years with AIS. We used SAPO with the thoracic markers in the form of angles (A) and distances (D) with involves structures like acromion, manubrium, xiphoid process, lower angle of the scapula, last false rib, anterior iliac spine process. Two experienced observers (A and B) analyzed the photos and all followed the same routine of analysis. Intraobserver and interobserver reproducibility was assessed by the Bland-Altman plot and intraclass correlation coefficient (ICC), while intraobserver and interobserver reliability was assessed by the T-Test and Wilcoxon's Test. A high repeatability index was obtained among the evaluations, with twelve of the sixteen variables considered as reliable in all statistical tests. The interobserver analyzes presented excellent correlation coefficients (ICC), showing good reliability for six of the sixteen variables proposed. The SAPO method presented good reproducibility and reliability for most of the thoracic markers created, showing that photogrammetry may be a complementary tool in the evaluation of thoracic alterations in patients with AIS.

Resumo A escoliose idiopática do adolescente (EIA) é uma deformidade tridimensional da coluna que gera alterações na biomecânica da caixa torácica. A fotogrametria digital permite o registro de mudanças sutis e a inter-relação entre partes do corpo humano que são difíceis de medir por outros meios. Objetivou-se criar ângulos e distâncias torácicas e avaliar a confiabilidade interobservador e intraobservador dessas medidas utilizando o Software de Avaliação Postural (SAPO) em pacientes com EIA. Este estudo transversal avaliou 30 indivíduos com idades entre 11 e 18 anos com EIA. Utilizamos o SAPO com os marcadores torácicos na forma de ângulos (A) e distâncias (D), envolvendo estruturas como: acrômio, manúbrio, processo xifóide, ângulo inferior da escápula, última costela falsa, processo da espinha ilíaca anterior. Dois observadores experientes (A e B) analisaram as fotos e todos seguiram a mesma rotina de análise. A reprodutibilidade intraobservador e interobservador foi avaliada pelo gráfico de Bland-Altman e coeficiente de correlação intraclasse (CCI), enquanto a confiabilidade intraobservador e interobservador foi avaliada pelo Teste T e Teste de Wilcoxon. Um alto índice de repetibilidade foi obtido entre as avaliações, com doze das dezesseis variáveis ​​consideradas confiáveis ​​em todos os testes estatísticos. As análises interobservadores apresentaram excelentes coeficientes de correlação (ICC), mostrando boa confiabilidade para seis das dezesseis variáveis ​​propostas. O método SAPO apresentou boa reprodutibilidade e confiabilidade para a maioria dos marcadores torácicos criados, mostrando que a fotogrametria pode ser uma ferramenta complementar na avaliação de alterações torácicas em pacientes com EIA.

Sundown syndrome in patients with Alzheimer's disease dementia.

The sundown syndrome is a complex neurobehavioral disorder in patients with dementia associated with high financial cost and significant caregiver burden. It is a multifactorial phenomenon with unclear pathophysiology, characterized by the presence of neuropsychiatric symptoms in the evening period. OBJECTIVE: To analyze the main neuropsychiatric symptoms, their correlation with one another, with comorbidities, and with time of day of greatest symptom intensity in patients with Alzheimer's disease dementia. METHODS: This is a cross-sectional, observational and explanatory study in which caregivers/relatives of elderly patients with dementia were interviewed using a structured tool called the Neuropsychiatric Inventory (NPI). RESULTS: The sample studied was composed of 38 patients, 60.5% female and 39.5% male, with mean age of 81±6 (67-94) years. A high frequency of neuropsychiatric symptoms in the evening period was observed, predominantly irritability (55.3%), nocturnal behavior (47.4%), and aggressiveness (42.1%). Only 36.8% of the family caregivers used non-pharmacological strategies. CONCLUSION: The frequency of neuropsychiatric symptoms was exacerbated in the evening among patients with Alzheimer's disease, especially for those behavioral symptoms that had a positive correlation with one another.

A síndrome do entardecer ou "pôr do sol" - "sundown syndrome" - é uma alteração neurocomportamental complexa em pacientes portadores de demência que geram custos financeiros excessivos e desgaste importante a seus cuidadores. Trata-se de um fenômeno multifatorial com fisiopatologia incerta, que caracteriza-se pela presença de sintomas neuropsiquiátricos no período vespertino. OBJETIVO: Analisar os principais sintomas neuropsiquiátricos, sua correlação entre si, com comorbidades e o horário do dia de maior intensidade destes em pacientes portadores de demência da doença de Alzheimer. MÉTODOS: Trata-se de um estudo analítico transversal, observacional e explanatório no qual foram entrevistados cuidadores/familiares de pacientes idosos portadores de demência utilizando uma ferramenta estruturada denominada Neuropsychiatric Inventory (NPI). RESULTADOS: A amostra estudada foi de 38 pacientes, 60,5% feminino e 39,5% masculino, com 81±6 (67-94) anos de idade. Foi observada uma alta frequência de sintomas neuropsiquiátricos no período vespertino, principalmente irritabilidade (55,3%), comportamento noturno (47,4%), agressividade (42,1%). Apenas 36,8% dos cuidadores familiares empregavam estratégias não farmacológicas. CONCLUSÃO: A frequência dos sintomas neuropsiquiátricos são exacerbados ao entardecer em pacientes com doença de Alzheimer; principalmente os sintomas comportamentais com correlação positiva entre si.

Avaliação microbiológica de coxa e sobrecoxa de frango comercializadas a granel em Sinop-MT / Microbiological evaluation of thigh and drumstick chicken sold in bulk in Sinop-MT

O consumo de carne de frango tem aumentado nos últimos anos, favorecido por diferentes fatores. No entanto, quando esse alimento é manipulado sem cuidados higiênicos pode ser fonte de agentes etiológicos causadores de enfermidades. O objetivo deste estudo foi avaliar a qualidade microbiológica de 60 amostras de carnes de frango industrial resfriadas in natura, coletadas de 20 estabelecimentos comerciais do município de Sinop Mato Grosso. As avaliações microbiológicas foram compostas por: enumeração de coliformes totais e termotolerantes, pesquisa de Escherichia coli e identificação de Salmonella spp. Em 100% das amostras foram encontrados coliformes totais. Seis amostras apresentaram valores de coliformes termotolerantes > 3NMP/g, porém não extrapolaram o padrão, 104NMP/g, exigido pela legislação brasileira. Das 60 amostras avaliadas, 8,3% apresentaram contaminação por E. coli e 8,3% estavam contaminadas por Salmonella spp. Em 30% dos mercados foram obtidas amostras contaminadas por E. coli (10%) e Salmonella spp. (20%), sendo consideradas impróprias para o consumo. Os sorovares de Salmonella spp. encontrados foram: Hadar, Minnesota, Heidelberg e Kentucky. Dessa forma, há a necessidade de que sejam aplicadas boas práticas de fabricação, higiene e manipulação de alimentos nos estabelecimentos a fim de reduzir a contaminação por microrganismos no produto final e os riscos para a saúde coletiva.(AU)

The chicken meat consumption has increased in recent years favored by different factors. However, when this food is manipulated without hygienic care, can be a source of etiological agents that cause diseases. The objective of this study was to evaluate the microbiological quality of 60 samples of fresh chilled chicken meat cooled in nature, collected from 20 commercial establishments in the municipalyti of Sinop Mato Grosso. The microbiological assessments were composed of: enumeration of total and thermotolerant coliforms, Escherichia coli research and identification of Salmonella spp. In 100% of the samples total coliforms were found. Six samples presented thermotolerant coliform values >3 NMP / g, but did not extrapolate the standard 104NMP / g required by brazilian legislation. Of the 60 samples evaluated, 8.3% presented contamination by E. coli and 8.3% were contaminated by Salmonella spp.. In 30% of markets were obtained contaminated samples by E. coli (10%) and Salmonella spp. (20%), being considered unsuitable for consumption.Theserovars of Salmonella spp. found were: Hadar, Minnesota, Heidelberg and Kentucky.In this way, there is a need for good manufacturing practices applied, hygiene and food handling in establishments in order to reduce contamination by microorganisms in the final product and the risks to collective health.(AU)

Sundown syndrome in patients with Alzheimer's disease dementia / Síndrome do entardecer em pacientes portadores de demência da doença de alzheimer

ABSTRACT The sundown syndrome is a complex neurobehavioral disorder in patients with dementia associated with high financial cost and significant caregiver burden. It is a multifactorial phenomenon with unclear pathophysiology, characterized by the presence of neuropsychiatric symptoms in the evening period. Objective: To analyze the main neuropsychiatric symptoms, their correlation with one another, with comorbidities, and with time of day of greatest symptom intensity in patients with Alzheimer's disease dementia. Methods: This is a cross-sectional, observational and explanatory study in which caregivers/relatives of elderly patients with dementia were interviewed using a structured tool called the Neuropsychiatric Inventory (NPI). Results: The sample studied was composed of 38 patients, 60.5% female and 39.5% male, with mean age of 81±6 (67-94) years. A high frequency of neuropsychiatric symptoms in the evening period was observed, predominantly irritability (55.3%), nocturnal behavior (47.4%), and aggressiveness (42.1%). Only 36.8% of the family caregivers used non-pharmacological strategies. Conclusion: The frequency of neuropsychiatric symptoms was exacerbated in the evening among patients with Alzheimer's disease, especially for those behavioral symptoms that had a positive correlation with one another.

RESUMO A síndrome do entardecer ou "pôr do sol" - "sundown syndrome" - é uma alteração neurocomportamental complexa em pacientes portadores de demência que geram custos financeiros excessivos e desgaste importante a seus cuidadores. Trata-se de um fenômeno multifatorial com fisiopatologia incerta, que caracteriza-se pela presença de sintomas neuropsiquiátricos no período vespertino. Objetivo: Analisar os principais sintomas neuropsiquiátricos, sua correlação entre si, com comorbidades e o horário do dia de maior intensidade destes em pacientes portadores de demência da doença de Alzheimer. Métodos: Trata-se de um estudo analítico transversal, observacional e explanatório no qual foram entrevistados cuidadores/familiares de pacientes idosos portadores de demência utilizando uma ferramenta estruturada denominada Neuropsychiatric Inventory (NPI). Resultados: A amostra estudada foi de 38 pacientes, 60,5% feminino e 39,5% masculino, com 81±6 (67-94) anos de idade. Foi observada uma alta frequência de sintomas neuropsiquiátricos no período vespertino, principalmente irritabilidade (55,3%), comportamento noturno (47,4%), agressividade (42,1%). Apenas 36,8% dos cuidadores familiares empregavam estratégias não farmacológicas. Conclusão: A frequência dos sintomas neuropsiquiátricos são exacerbados ao entardecer em pacientes com doença de Alzheimer; principalmente os sintomas comportamentais com correlação positiva entre si.