Differences of ventilatory muscle recruitment and work of breathing in COPD and interstitial lung disease during exercise: a comprehensive evaluation.

Introduction: COPD and interstitial lung disease (ILD) are significant chronic respiratory disorders, impacting quality of life. Respiratory muscle roles and differences remain not entirely clear. The objective of the present study was to evaluate the degree of recruitment of the respiratory muscles and the work of breathing in COPD and ILD during exercise. Methods: We compared the sensory-mechanical relationships in COPD, ILD and healthy controls (n=20 each). They performed pulmonary function, noninvasive and invasive respiratory muscle strength, surface electromyography and work-of-breathing assessments. Results: COPD and ILD did not show lower static muscle strength compared to controls, but did show poor performance in the exercise test with increased transdiaphragmatic pressure (P di). In ILD, there was a higher increase in oesophageal pressure and a lower gastric pressure (P ga) on inspiration; in COPD, there was a significant increase in P ga on inspiration. In ILD, there is greater recruitment of accessory inspiratory muscles, whereas in COPD, there is marked use of both inspiratory and expiratory muscles. The neuromechanical inefficiency (increased neural respiratory drive without the corresponding tidal volume) was found in both diseases. In COPD, there is a considerable increase in elastic work to overcome intrinsic positive end-expiratory pressure (PEEPi) and expiratory work of breathing, whereas in ILD, non-PEEPi elastic work of breathing is the highest part of the total work of breathing. Conclusions: Early and increased activity of the respiratory muscles and work-of-breathing components significantly contribute to dyspnoea, exercise intolerance and neuromechanical inefficiency of ventilation in COPD and ILD. The mechanisms of P di generation were different between diseases.

Unilateral diaphragm paralysis: a dysfunction restricted not just to one hemidiaphragm.

BACKGROUND: Most patients with unilateral diaphragm paralysis (UDP) have unexplained dyspnea, exercise limitations, and reduction in inspiratory muscle capacity. We aimed to evaluate the generation of pressure in each hemidiaphragm separately and its contribution to overall inspiratory strength. METHODS: Twenty-seven patients, 9 in right paralysis group (RP) and 18 in left paralysis group (LP), with forced vital capacity (FVC) < 80% pred, and 20 healthy controls (CG), with forced expiratory volume in 1 s (FEV1) > 80% pred and FVC > 80% pred, were evaluated for lung function, maximal inspiratory (MIP) and expiratory (MEP) pressure measurements, diaphragm ultrasound, and transdiaphragmatic pressure during magnetic phrenic nerve stimulation (PdiTw). RESULTS: RP and LP had significant inspiratory muscle weakness compared to controls, detected by MIP (- 57.4 ± 16.9 for RP; - 67.1 ± 28.5 for LP and - 103.1 ± 30.4 cmH2O for CG) and also by PdiTW (5.7 ± 4 for RP; 4.8 ± 2.3 for LP and 15.3 ± 5.7 cmH2O for CG). The PdiTw was reduced even when the non-paralyzed hemidiaphragm was stimulated, mainly due to the low contribution of gastric pressure (around 30%), regardless of whether the paralysis was in the right or left hemidiaphragm. On the other hand, in CG, esophagic and gastric pressures had similar contribution to the overall Pdi (around 50%). Comparing both paralyzed and non-paralyzed hemidiaphragms, the mobility during quiet and deep breathing, and thickness at functional residual capacity (FRC) and total lung capacity (TLC), were significantly reduced in paralyzed hemidiaphragm. In addition, thickness fraction was extremely diminished when contrasted with the non-paralyzed hemidiaphragm. CONCLUSIONS: In symptomatic patients with UDP, global inspiratory strength is reduced not only due to weakness in the paralyzed hemidiaphragm but also to impairment in the pressure generated by the non-paralyzed hemidiaphragm.

Dental Functionality: construction and validation of an oral health indicator for institutionalized elderly persons in the city of Natal, Rio Grande do Norte / Funcionalidade Dentária: construção e validação de um indicador de saúde bucal para idosos institucionalizados da cidade do Natal, RN

Abstract Objective: to suggest a composite indicator that identifies the oral health condition of institutionalized elderly persons. Method: an observational and cross-sectional study was performed. A total of 315 elderly persons were investigated in long-stay care facilities for the elderly in the city of Natal, Rio Grande do Norte, Brazil. Such individuals underwent an epidemiological evaluation of their oral health conditions, based on the DMFT index, CPI and the PAL (periodontal attachment loss) index. Factor analysis was used to identify a relatively small number of common factors by principal component analysis. Results: five oral health variables were included in factor analysis, and using the Kaiser criterion, which considers the percentage of variance explained by the factors, a single factor which together explained 79.7% of the total variance of the variables included in the analysis model was selected. This factor was analyzed and interpreted according to the dimension to which it related, and was entitled the factor of Dental Functionality. Conclusion: this factor generated an objective indicator to characterize the oral health of the elderly in long-term care facilities for the elderly of Natal, Rio Grande do Norte, and represents a parameter for studies of the oral health outcomes of this elderly population. It also revealed a change in the dental profile of this population with more teeth present in the mouth and a reduction in edentulism. AU

Resumo Objetivo: sugerir um indicador composto que discrimine a condição de saúde bucal dos idosos institucionalizados. Método: o estudo se caracteriza por ser do tipo observacional e transversal. Foram investigados 315 idosos nas Instituições de Longa Permanência para Idosos (ILPI) da cidade do Natal, RN, Brasil. Esses idosos foram avaliados quanto às condições de saúde bucal, a partir dos índices CPO-d, CPI e PIP. Uma análise fatorial buscou identificar um número relativamente pequeno de fatores comuns, por meio da análise de componentes principais. Resultados: foram incluídas na análise fatorial cinco variáveis de saúde bucal e, a partir do critério de Kaiser que considera o percentual de variância explicado pelos fatores, foi selecionado um fator que, em conjunto, explicou 79,7% da variância total das variáveis incluídas no modelo de análise. Tal fator foi analisado e interpretado segundo a dimensão a qual se referia, denominando-se o fator de Funcionalidade Dentária. Conclusão: esse fator foi capaz de gerar um indicador objetivo que caracteriza a saúde bucal do idoso das ILPI de Natal, RN, servindo como parâmetro para estudos que tenham como desfecho a saúde bucal dessa população idosa, além de mostrar uma mudança no perfil dessa população com o aumento de dentes em boca e diminuição do edentulismo. AU

Inequality in the characteristics of the institutionalized elderly in the city of Natal, Brazil. / Desigualdade no perfil dos idosos institucionalizados na cidade de Natal, Brasil.

The aim of this study was to describe the differences in the profile of elderly residents in non-profit and for-profit Long-Term Residential Institutions for the Elderly (ILPI). We visited 12 ILPIs in the city of Natal, Rio Grande do Norte. Six of them were non-profits housing 260 elderly, and six were for-profit institutions, housing 127 elderly. The unequal characteristics of the elderly in these facilities are based on socioeconomic data and the reason for institutionalization. The data was submitted to Chi-squared or Student's t, with a significance level of 5%. Illiterate, single, black or brown, individuals with no retirement/pension or healthcare plan, no children and no visitors who purchase something outside the institution with their own money are associated with non-profit ILPIs. When analyzing the reasons for institutionalization, family conflict, abandonment and being homeless were associated with the elderly institutionalized in non-profit ILPIs. The main reason for the elderly institutionalized in for-profit ILPIs was "being ill". The conditions of the elderly in non-profit ILPIs were poorer, reflecting the social inequality these subjects experienced throughout their lifetime.

Desigualdade no perfil dos idosos institucionalizados na cidade de Natal, Brasil / Inequality in the characteristics of the institutionalized elderly in the city of Natal, Brazil

Resumo Objetivou-se verificar as desigualdades no perfil dos idosos residentes em Instituições de Longa Permanência para Idosos (ILPIs) com e sem fins lucrativos. Foram visitadas 12 ILPIs na cidade do Natal-RN, sendo 6 sem fins lucrativos com 260 idosos avaliados e 6 com fins lucrativos e 127 idosos. A caracterização das desigualdades no perfil dos idosos se deu a partir de dados socioeconômicos e motivo da institucionalização. Os dados foram submetidos aos testes Qui-quadrado ou t de Student com nível de significância de 5%. Como resultado, aqueles idosos analfabetos, solteiros, negros e pardos, não aposentados, sem plano de saúde, sem filhos, que não recebem visitas e que compram algo fora da instituição com o próprio dinheiro, estavam associados às ILPIs sem fins lucrativos. Ao analisar os motivos que levaram o idoso a ser institucionalizado, os conflitos familiares, o abandono e o fato de não possuir lugar para morar estiveram associados às ILPIs sem fins lucrativos. Apenas a condição “estar doente” prevaleceu como motivo principal de institucionalização em ILPIs com fins lucrativos. As condições mais desfavoráveis foram dos idosos residentes em ILPIs sem fins lucrativos, o que demonstram o reflexo da desigualdade social durante a vida desses idosos.

Abstract The aim of this study was to describe the differences in the profile of elderly residents in non-profit and for-profit Long-Term Residential Institutions for the Elderly (ILPI). We visited 12 ILPIs in the city of Natal, Rio Grande do Norte. Six of them were non-profits housing 260 elderly, and six were for-profit institutions, housing 127 elderly. The unequal characteristics of the elderly in these facilities are based on socioeconomic data and the reason for institutionalization. The data was submitted to Chi-squared or Student’s t, with a significance level of 5%. Illiterate, single, black or brown, individuals with no retirement/pension or healthcare plan, no children and no visitors who purchase something outside the institution with their own money are associated with non-profit ILPIs. When analyzing the reasons for institutionalization, family conflict, abandonment and being homeless were associated with the elderly institutionalized in non-profit ILPIs. The main reason for the elderly institutionalized in for-profit ILPIs was “being ill”. The conditions of the elderly in non-profit ILPIs were poorer, reflecting the social inequality these subjects experienced throughout their lifetime.

Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry.

BACKGROUND: With the emergence of strains resistant to conventional antibiotics, it is important to carry studies using alternative methods to control these microorganisms causing important infections, such as the use of products of plant origin that has demonstrated effective antimicrobial activity besides biocompatibility. Therefore, this study aimed to evaluate the antimicrobial activity of plant extracts of Equisetum arvense L., Glycyrrhiza glabra L., Punica granatum L. and Stryphnodendron barbatimam Mart. against Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Candida albicans, Candida tropicalis, and Candida glabrata, and to analyze the cytotoxicity of these extracts in cultured murine macrophages (RAW 264.7). METHODS: Antimicrobial activity of plant extracts was evaluated by microdilution method based on Clinical and Laboratory Standards Institute (CLSI), M7-A6 and M27-A2 standards. The cytotoxicity of concentrations that eliminated the microorganisms was evaluated by MTT colorimetric method and by quantification of proinflammatory cytokines (IL-1ß and TNF-α) using ELISA. RESULTS: In determining the minimum microbicidal concentration, E. arvense L., P. granatum L., and S. barbatimam Mart. extracts at a concentration of 50 mg/mL and G. glabra L. extract at a concentration of 100 mg/mL, were effective against all microorganisms tested. Regarding cell viability, values were 48% for E. arvense L., 76% for P. granatum L, 86% for S. barbatimam Mart. and 79% for G. glabra L. at the same concentrations. About cytokine production after stimulation with the most effective concentrations of the extracts, there was a significant increase of IL-1ß in macrophage cultures treated with S. barbatimam Mart. (3.98 pg/mL) and P. granatum L. (7.72 pg/mL) compared to control (2.20 pg/mL) and a significant decrease of TNF-α was observed in cultures treated with G. glabra L. (4.92 pg/mL), S. barbatimam Mart. (0.85 pg/mL), E. arvense L. (0.83 pg/mL), and P. granatum L. (0.00 pg/mL) when compared to control (41.96 pg/mL). CONCLUSIONS: All plant extracts were effective against the microorganisms tested. The G. glabra L. extract exhibited least cytotoxicity and the E. arvense L. extract was the most cytotoxic.

Upper limb exercises using varied workloads and their association with dynamic hyperinflation in patients with COPD.

BACKGROUND: Increased ventilation during upper limb exercises (ULE) in patients with COPD is associated with dynamic hyperinflation (DH) and a decrease in inspiratory capacity (IC). The best level of ULE load training is still unknown. Our objective was to evaluate the dynamic hyperinflation development during ULE using three constant workloads. METHODS: This was a prospective, randomized protocol involving 24 patients with severe COPD (FEV(1) < 50%) performing an endurance symptom-limited arm exercise of up to 20 min in an arm cycloergometer with different workloads (50%, 65%, and 80% of the maximal load). Ventilation, metabolic, and lung function variables (static IC pre-exercise and postexercise) were measured. RESULTS: DH was observed during exercises with 65% (-0.23 L) and 80% (-0.29 L) workloads (P < .0001). Total time of exercise with 80% workload (7.6 min) was shorter than with 50% (12.5 min) (P < .0005) and with 65% (10.1 min; not significant). Oxygen consumption percent predicted (VO(2)) (P < .01) was lower with 50% workload than with 80%. Eighty percent workload showed lower work efficiency (VO(2) [mL/kg]/exercise time) than the other two workloads (P < .0001). CONCLUSION: Different workloads during upper limb exercises showed a direct influence over dynamic hyperinflation and the endurance exercise duration.