RESUMEN
Previously, it was isolated from the fruiting bodies of the gilled mushroom Pholiota spumosa (Basidiomycetes, Strophariaceae), putrescine-1,4-dicinnamide, a phenylpropanoid derivative conjugated with polyamine putrescine never isolated before as a natural compound. Recently, polyamine analogs that are similar in structure to the natural polyamines but that cannot mimic their functions that are essential for cellular growth and differentiation, have shown antitumor activity in several types of human cancer cells. Therefore, we have now investigated the response of DU-145 cells, a well characterized androgen-independent human prostate cancer (PCA) cell line, to this phenylpropanoid derivative. The results presented here demonstrate that putrescine-1,4-dicinnamide, as suggested for polyamine analogs synthesized artificially, inhibits the cell growth of cancer cells inducing apoptosis cell death, mediated, at least in part, by the activation of caspase cascades, that at higher doses shift to necrosis, through the increase of reactive oxygen species (ROS) generation.
Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Basidiomycota , Proliferación Celular/efectos de los fármacos , Fitoterapia , Putrescina/análogos & derivados , Antineoplásicos Fitogénicos/administración & dosificación , Antineoplásicos Fitogénicos/uso terapéutico , Línea Celular Tumoral/efectos de los fármacos , Línea Celular Tumoral/metabolismo , Cuerpos Fructíferos de los Hongos , Humanos , Masculino , Neoplasias de la Próstata/tratamiento farmacológico , Neoplasias de la Próstata/patología , Putrescina/administración & dosificación , Putrescina/farmacología , Putrescina/uso terapéuticoRESUMEN
Three new lanostane triterpenes, hebelomic acids B[4], E[5], and F[6], were isolated from the inedible mushroom Hebeloma senescens. The latter two compounds are acyl derivatives of the new triterpene senescensol (12-deoxycrustulinol) [12]. The structures of compounds 4-6, including the absolute configuration of the 3-hydroxy-3-methylglutaric acid moiety, were established on the basis of spectral and chemical evidence.
Asunto(s)
Agaricales/química , Antiinfecciosos/química , Triterpenos/química , Animales , Antibacterianos , Antiinfecciosos/farmacología , Antiinfecciosos/toxicidad , Artemia , Bacterias/efectos de los fármacos , Candida albicans/efectos de los fármacos , Dosificación Letal Mediana , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Espectrofotometría Infrarroja , Triterpenos/farmacología , Triterpenos/toxicidadRESUMEN
2-Geranylgeranyl-1,4-dihydroxybenzene [1] and a mixture of fatty acid esters of this phenol were isolated from the fruiting bodies of Lactarius lignyotus. Compound 1 was highly active in the brine shrimp test and showed significant inhibitory activity on DNA, RNA, and protein synthesis in HeLa and HL-60 cell lines.
Asunto(s)
Basidiomycota/química , Diterpenos , Terpenos/aislamiento & purificación , ADN de Neoplasias/biosíntesis , Ensayos de Selección de Medicamentos Antitumorales , Células HeLa , Humanos , Leucina/metabolismo , Proteínas de Neoplasias/biosíntesis , ARN Neoplásico/biosíntesis , Terpenos/química , Terpenos/farmacología , Timidina/metabolismo , Células Tumorales Cultivadas , Uridina/metabolismoRESUMEN
This study of lipids of Lactarius uvidus has revealed the presence of new fatty acid esters of uvidin A [2a-d] and drimenol [6a-d]. Treatment of uvidin A [1] with base induced a Favorskii-like rearrangement leading to compounds with a new sesquiterpenoid skeleton. Uvidin A [1] showed insect antifeedant and cytotoxic activities.
Asunto(s)
Basidiomycota/química , Ácidos Grasos/aislamiento & purificación , Sesquiterpenos/aislamiento & purificación , Animales , Antineoplásicos/aislamiento & purificación , Antineoplásicos/farmacología , Artemia , Ensayos de Selección de Medicamentos Antitumorales , Ésteres , Ácidos Grasos/farmacología , Conducta Alimentaria/efectos de los fármacos , Humanos , Insectos , Sesquiterpenos/farmacología , Células Tumorales CultivadasRESUMEN
Six flavonol glycosides and ent-epicatechin were isolated from Dryas octopetala and their structures elucidated by chemical and spectroscopic methods. Two new flavonoids, 3-O-alpha-L-arabinofuranosyl-8-methoxyquercetin (2) and 3-O-beta-galactopyranosyl-8-methoxykaempferol (6), were identified along with 3-O-beta-D-galactopyranosylquercetin (hyperin) (3), 3-O-alpha-L-arabinofuranosylquercetin (avicularin) (5), 3-O-beta-L-arabinopyranosylquercetin (4), and 3-O-beta-D-galactopyranosyl-8-methoxyquercetin (1).
Asunto(s)
Flavonoides/aislamiento & purificación , Plantas Medicinales/análisis , Fenómenos Químicos , Química , Quercetina/análogos & derivados , Quercetina/aislamiento & purificaciónRESUMEN
The composition of the essential oil of Aframomum giganteum was investigated by GC-MS. Forty seven terpenes were identified, the oil contained a high percentage of linalool and linalyl acetate which are mainly responsible for the smell of the A. giganteum stems. Fatty acids, paraffins, n-octanol, n-nonanol and guaiacol were also identified.