RESUMEN
The amino acid sequence of the known strain HXB-2 belonging to subtype B is used as a reference strain matrix to determine HIV genotypic resistance by the sequencing technique. However, numerous studies have ascertained that HIV non-B subtypes have been widely accepted in Russia. The authors' data show that the significance of low score mutations in the Russian populations of HIV-1 is yet to be explained, unquestionably, arouses great scientific interest, and calls for further study.
Asunto(s)
Fármacos Anti-VIH/farmacología , VIH-1/efectos de los fármacos , Adolescente , Adulto , Niño , Preescolar , Farmacorresistencia Viral , Femenino , VIH-1/genética , Humanos , Lactante , Masculino , Persona de Mediana Edad , Mutación , Siberia , Adulto JovenRESUMEN
In order to detect mutations in protease and reverse transcriptase regions determining the resistance of human immunodeficiency virus (HIV) to antiretroviral drugs, sequencing of pol gene was used. Itwas concluded that HIV epidemic in Yamal region is characterized by heterogeneity of HIV-1 subtype population with trend to domination of recombinant and ground variants and spreading of mutant Mut-77 strain. Obtained results demonstrate the possibility of identification of mutations as epidemiological markers which permits their use for epidemiologic investigations.
Asunto(s)
Farmacorresistencia Viral/genética , Infecciones por VIH/epidemiología , Infecciones por VIH/virología , VIH-1/genética , Productos del Gen pol del Virus de la Inmunodeficiencia Humana/genética , Adulto , Fármacos Anti-VIH/farmacología , Análisis Mutacional de ADN , Femenino , Variación Genética , Proteasa del VIH/genética , Transcriptasa Inversa del VIH/genética , VIH-1/clasificación , VIH-1/aislamiento & purificación , Humanos , Masculino , Mutación , Recombinación Genética , Factores de Riesgo , Siberia/epidemiología , Abuso de Sustancias por Vía IntravenosaRESUMEN
The comparative evaluation of the reactogenic and immunogenic properties of vaccine "Euvax B" in adolescents aged 16-18 years was carried out in factory housing estate Lvovsky, the Podolsk district, the Moscow region, and in the Yamalo-Nenets Autonomous District (AD): in towns Salekhard, Noyabrsk, Muravlenko, in housing estate Tarko-Sale. The evaluation demonstrated that the use of this vaccine in a dose of 20 mg/ml produced no postvaccinal complications and the frequency of postvaccinal reactions was insignificant. Postvaccinal anti-HBs were detected, on the average, in protective concentrations in 98.4 +/- 1.8% of the vaccines (99.0 +/- 1.9% in the Moscow region, 97.7 +/- 3.2% in the Yamalo-Nenets AD). In most of the vaccinated adolescents high concentrations of postvacinal anti-HBs were detected: in 51.4 +/- 9.7% of the vaccinees in the Moscow region and in 89.7 +/- 6.4% in the Yamalo-Nenets AD they exceeded 1,000 I.U./lit. The conclusion was made that in the territories located in subarctic latitudes vaccinal prophylaxis against hepatitis B was safe and highly effective and have no limitations connected with the conditions of the Far North.
