[Binding sites for A2 and A24 monoclonal antibodies on the alpha-latrotoxin molecule]. / Uchastki uznavaniia monoklonal'nykh antitel A4 i A24 v molekule alpha-latrotoksina.

alpha-Latrotoxin (alpha-LTX) binding sites to functionally active monoclonal antibodies (MA) A4 and A24 were localized using three approaches: hydrolysis of the toxin followed by the N-terminal sequencing of immunoreactive peptides; the study of antibody interaction with several recombinant alpha-LTX fragments; and Western immunoblotting of synthetic overlapping peptides (6-8 aa) whose structures correspond to that of the immunoreactive alpha-LTX fragment. It was shown that the MA A4 epitope is located within the F234-M294 protein fragment and that of MA A24 interacts with the fragment 347FDKDIT352.

[Molecular cloning and primary structure of cDNA fragment for alpha-latrocrustatoxin from black widow spider venom]. / Molekuliarnoe klonirovanie i opredelenie pervichnoi struktury fragmenta kDNK alpha-latrokrustotoksina iz iada pauka karakurta.

A fragment of the structural gene of alpha-latrocrustotoxin, a new representative of latrotoxins from black widow spider venom, was cloned. The fragment (1191 bp) was obtained by means of PCR based on the data obtained by sequencing tryptic peptides of the toxin. The fragment codes for a 397-aa sequence. The encoded polypeptide is the C-terminal fragment of the toxin central domain that presumably contains a site responsible for the toxin species specificity. The structural similarity of this fragment to the corresponding fragments of other latrotoxins was studied.